Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,396,385	C→T	6.7%	R1091H (CGC→CAC)	PP_2100 ←	two‑component system sensor protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,396,385	0	C	T	6.7%	177.5 / 2.4	61	R1091H (CGC→CAC)	PP_2100	two‑component system sensor protein
Reads supporting (aligned to +/- strand):  ref base C (30/26);  new base T (2/2);  total (33/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

GCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACTTCCGGCTGTGCGTTGGCGGTATAGCCGA  >  NC_002947/2396243‑2396532                                                                                                                                                |                                                                                                                                                      gccgcTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCggg                                                                                                                                                 >  8:295121/1‑149 (MQ=255) gccgcTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCggg                                                                                                                                                 <  7:148101/149‑1 (MQ=255)   cgcTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCggggg                                                                                                                                               >  8:34229/1‑149 (MQ=255)      tGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGt                                                                                                                                            <  6:319507/149‑1 (MQ=255)           tgtTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTg                                                                                                                                       >  4:275848/1‑149 (MQ=255)                    tGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCgg                                                                                                                            >  7:272021/1‑149 (MQ=255)                      aaGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGc                                                                                                                          <  5:66907/149‑1 (MQ=255)                       aGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGTGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGc                                                                                                                          >  6:1700/1‑148 (MQ=255)                       aGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGTGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGc                                                                                                                          >  2:49373/1‑148 (MQ=255)                           ccATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATg                                                                                                                     >  4:80512/1‑149 (MQ=255)                             aTCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATgc                                                                                                                    >  2:92863/1‑148 (MQ=255)                                aGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATgccagc                                                                                                                <  7:295121/149‑1 (MQ=255)                                  gCTCGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCATGCGTCGGGTGCGTTGCTG‑GCG‑GCCCGGGCGAATgccacc                                                                                                                >  8:98471/1‑145 (MQ=255)                                              ggCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGTGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGa                                                                                                  <  5:1700/149‑1 (MQ=255)                                              ggCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGCTCAGGCCCGACAACCGCGGCCACGCCGTGCACCT‑GGTACACCTTGGGTAGGGGGCGTTGCTG‑GCG‑GCGCCGGCGAATGCAAGCCACGCcctggct                                                                                                    >  1:242474/1‑147 (MQ=255)                                               gCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGTGTCGGGGGCGTTGCTGTGCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGa                                                                                                  <  1:49373/149‑1 (MQ=255)                                               gCCTGAAGGCTCTGGTGCAAGGTTTCCAGCAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTCCTTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGa                                                                                                  <  1:92863/149‑1 (MQ=255)                                                 cTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAggg                                                                                               >  3:43881/1‑149 (MQ=255)                                                 cTGAAGGCTCGGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGCGcctggctggcat                                                                                               <  7:121229/149‑5 (MQ=255)                                                    aaGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTg‑gcg                                                                                                                                   >  6:323478/1‑111 (MQ=255)                                                    aaGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTg‑gcg                                                                                                                                   <  5:323478/111‑1 (MQ=255)                                                    aaGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGa                                                                                                  <  8:82653/143‑1 (MQ=255)                                                    aaGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGa                                                                                                  >  7:82653/1‑143 (MQ=255)                                                         tctGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAgg                                                                                       >  3:251087/1‑149 (MQ=255)                                                         tctGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAgg                                                                                       >  3:48903/1‑149 (MQ=255)                                                               gCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATg                                                                                 >  7:233505/1‑149 (MQ=255)                                                                cAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAgg                                                                                                >  6:131593/1‑133 (MQ=255)                                                                cAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAgg                                                                                                <  5:131593/133‑1 (MQ=255)                                                                 aaGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATggg                                                                               >  3:172068/1‑149 (MQ=255)                                                                  aGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCt                                                                                     >  8:263556/1‑142 (MQ=255)                                                                  aGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCt                                                                                     <  7:263556/142‑1 (MQ=255)                                                                    gTTTCCAGGAAGCGCTGACGGGCAACCGGGGCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCtt                                                                            <  1:288761/149‑1 (MQ=255)                                                                     ttgccATGAAGCGCTGACGGTCAACCGGGTCAGGCCGGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGTGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCTCTGGCTGAGGGTGCTCAGGCTGATGGGCtt                                                                            <  7:98471/145‑1 (MQ=255)                                                                       tCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGAGCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGa                                                                          <  4:48903/148‑1 (MQ=255)                                                                        ccAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAg                                                                         >  5:105948/1‑148 (MQ=255)                                                                         cAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCGCGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAgg                                                                                                <  1:206518/125‑1 (MQ=255)                                                                         cAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAgg                                                                                                >  2:206518/1‑124 (MQ=255)                                                                                cgcTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAgtc                                                                <  8:233505/149‑1 (MQ=255)                                                                                 gcTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAgtcg                                                               >  6:282425/1‑149 (MQ=255)                                                                                  cTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAgtcgt                                                              >  4:237313/1‑149 (MQ=255)                                                                                       ggTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGc                                                                                            >  1:8241/1‑114 (MQ=255)                                                                                       ggTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGc                                                                                            <  2:8241/114‑1 (MQ=255)                                                                                       ggTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAgtcgtc                                                             <  8:189430/145‑1 (MQ=255)                                                                                       ggTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAgtcgtc                                                             >  7:189430/1‑145 (MQ=255)                                                                                        gTCAACCGTGTCAGGCCCGACCACGGCGGCCAGGGCGTCCAGCT‑GGTACTGGTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGGGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGc                                                        <  4:96353/149‑1 (MQ=255)                                                                                           aaCCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCcgg                                                     >  6:33002/1‑149 (MQ=255)                                                                                           aaCCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCcg                                                      <  4:43881/148‑1 (MQ=255)                                                                                               gggTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATgcca                                                                                                                  >  4:194885/1‑84 (MQ=255)                                                                                               gggTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATgcca                                                                                                                  <  3:194885/84‑1 (MQ=255)                                                                                                ggTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGt                                                 >  4:130870/1‑148 (MQ=255)                                                                                                        ccGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGa                                                                          <  1:319476/115‑1 (MQ=255)                                                                                                        ccGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGa                                                                          >  2:319476/1‑115 (MQ=255)                                                                                                          gACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTgc                                      <  4:172068/149‑1 (MQ=255)                                                                                                           aCCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTgcg                                     <  3:80512/149‑1 (MQ=255)                                                                                                             cACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTgcggc                                   >  1:316041/1‑149 (MQ=255)                                                                                                               cggcggCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGt                                 <  8:272021/149‑1 (MQ=255)                                                                                                                ggcggcCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTa                                >  8:28250/1‑149 (MQ=255)                                                                                                                       aGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACTTCCgg                         <  5:282425/149‑1 (MQ=255)                                                                                                                       aGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACTTCCgg                         <  4:251087/149‑1 (MQ=255)                                                                                                                                  cT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACTTCCGGCTGTGCGTTgg              >  5:275014/1‑149 (MQ=255)                                                                                                                                            cTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACTTCCGGCTGTGCGTTGGCGGTATAGc     >  5:68289/1‑149 (MQ=255)                                                                                                                                                cGTCGGGGTGGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACTTCCGGCTGTGCGTTGGCGGTATAGCCGa  <  2:242474/148‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                      GCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCT‑GGTACAGCTTGCGTCGGGGGCGTTGCTG‑GCG‑GCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGGTGCTCAGGCTGATGGGCTTGAGCAGACAGTCGTCCATGCCGGCGGTCAGGCACTTGCGGCGTACTTCCGGCTGTGCGTTGGCGGTATAGCCGA  >  NC_002947/2396243‑2396532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑