Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,405,232 G→T 10.0% V77L (GTG→TTG)  PP_2108 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,405,2320GT10.0% 152.3 / 6.8 50V77L (GTG→TTG) PP_2108hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (21/24);  new base T (2/3);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.98e-01

CTCGAAGCCAGGGCCAACCTGCAATTACTGTGTGGTGCGCTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACG  >  NC_002947/2405088‑2405375
                                                                                                                                                 |                                                                                                                                               
cTCGAAGCCAGGGCCAACCTGCAATTACTGTGTGGTGCGCTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGc                                                                                                                                              >  2:76387/1‑148 (MQ=255)
      gCCAGGGCCAACCTGCAATTACTGTGTGGTGCGCTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGAGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGc                                                                                                                                        <  1:76387/149‑1 (MQ=255)
      gCCAGGGCCAACCTGCAATTACTGTGTGGTGCGCTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCt                                                                                                                                       >  7:39510/1‑149 (MQ=255)
             ccAACCTGCAATTACTGTGTGGTGCGCAGCAACAGGTACGTGCGTTGTTTGACTCGCCCATCAACATCAGCAGCGGGTATCTCAGCGAAAGG‑GTCAATCGCTTGATCGGTGCAGCCTCCGATACCCAGCACGTGCAAGGTCTGgcggcg                                                                                                                                >  8:169020/1‑149 (MQ=255)
              cAACCTGCAATTACTGTGTGGTGCGCTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCCCTTGCACGTGCTggctatcg                                                                                                                               <  5:70301/149‑6 (MQ=255)
              cAACCTGCAATTACTGTGTGGTGCGCTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGgcggcg                                                                                                                                >  8:246069/1‑148 (MQ=255)
                                   tgCGCTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCa                                                                                                          <  7:246069/149‑1 (MQ=255)
                                    gcgcTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAg                                                                                                         >  6:151646/1‑149 (MQ=255)
                                          gAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGt                                                                                                            <  6:27293/140‑1 (MQ=255)
                                          gAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGt                                                                                                            >  5:27293/1‑140 (MQ=255)
                                               ggTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCgaga                                                                                              <  5:151646/149‑1 (MQ=255)
                                                     tGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTcc                                                                                        >  6:184777/1‑149 (MQ=255)
                                                      gCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCg                                                                                       >  5:75164/1‑149 (MQ=255)
                                                        gttgttTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAgg                                                                                                             <  7:2264/125‑1 (MQ=255)
                                                        gttgttTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAgg                                                                                                             >  8:2264/1‑125 (MQ=255)
                                                         ttgttTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGc                                                                                     >  5:236423/1‑148 (MQ=255)
                                                                       catcatcatCAGCAGCGGGTATCGCAGCGAAAGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTg                                                                      <  2:209798/149‑1 (MQ=255)
                                                                           atcctcaGCTGCGGGTATCGCAGCTAAAGG‑GTCAAGAGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCACGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGt                                                                  <  8:268182/149‑1 (MQ=255)
                                                                              atcaGCAGCGGGTATCGCAGCGAAAGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAg                                                                        <  6:52291/140‑1 (MQ=255)
                                                                              atcaGCAGCGGGTATCGCAGCGAAAGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAg                                                                        >  5:52291/1‑140 (MQ=255)
                                                                               tcaGCAGCGGGTATCGCAGCGAAAGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGccc                                                              >  4:265581/1‑149 (MQ=255)
                                                                                 agcagcGGGTATCGCAGCGAAAGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCtt                                                            <  2:71537/149‑1 (MQ=255)
                                                                                 agcagcGGGTATCGCAGCGAAAGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCt                                                             <  7:265007/148‑1 (MQ=255)
                                                                                   cagcGGGTATCGCAGCGAAAGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTc                                                           >  7:290612/1‑148 (MQ=255)
                                                                                   cagcGGGTATCGCAGCGAAAGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCg                                                          <  3:169882/149‑1 (MQ=255)
                                                                                     gcGGGTATCGCAGCGAAAGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGAt                                                        <  6:75164/149‑1 (MQ=255)
                                                                                      cGGGTATCGCAGCGAAAGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCga                                                                                                <  3:61157/108‑1 (MQ=255)
                                                                                      cGGGTATCGCAGCGAAAGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCga                                                                                                >  4:61157/1‑108 (MQ=255)
                                                                                         gTATCGCAGCGAAAGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCg                                                                    <  6:246630/133‑1 (MQ=255)
                                                                                         gTATCGCAGCGAAAGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCg                                                                    >  5:246630/1‑133 (MQ=255)
                                                                                         gTATCGCAGCGAAAGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGc                                                    <  6:236423/149‑1 (MQ=255)
                                                                                                      aGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAAtt                                       <  8:39510/149‑1 (MQ=255)
                                                                                                       gg‑gTCAATCGGTTGTTCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACCTCACGGTGATCGAGGTCAGCCCTAGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTc                                      <  7:169020/149‑1 (MQ=255)
                                                                                                        g‑gTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCg                                     <  3:265581/149‑1 (MQ=255)
                                                                                                          gTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCg                                     >  7:106231/1‑148 (MQ=255)
                                                                                                           tCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGAc                                   <  5:184777/149‑1 (MQ=255)
                                                                                                               tCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCg                                                                                                                >  8:104236/1‑68 (MQ=255)
                                                                                                               tCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCg                                                                                                                <  7:104236/68‑1 (MQ=255)
                                                                                                                  gTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTc                                      >  6:156889/1‑139 (MQ=255)
                                                                                                                  gTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTc                                      <  5:156889/139‑1 (MQ=255)
                                                                                                                       tCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGc                                                                                                        >  4:287703/1‑68 (MQ=255)
                                                                                                                       tCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGc                                                                                                        <  3:287703/68‑1 (MQ=255)
                                                                                                                          gTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTg                    <  8:290612/149‑1 (MQ=255)
                                                                                                                                      gccgCCAGCCCTTGCACGTGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGAcgcgc        >  7:6636/4‑149 (MQ=255)
                                                                                                                                      aTAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGAcgcgc        >  2:90052/1‑149 (MQ=255)
                                                                                                                                         gCCAGCCCTTGCACGTGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGc     <  8:6636/149‑1 (MQ=255)
                                                                                                                                         gCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCgg      <  1:90052/148‑1 (MQ=255)
                                                                                                                                          ccAGCCCTTGCACGTGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCa    >  8:58267/1‑149 (MQ=255)
                                                                                                                                           cAGCCCTTGCACGTGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCAc   <  7:58267/149‑1 (MQ=255)
                                                                                                                                            aGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCAcg  >  1:176192/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CTCGAAGCCAGGGCCAACCTGCAATTACTGTGTGGTGCGCTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGG‑GTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACG  >  NC_002947/2405088‑2405375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑