| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,414,748 | C→A | 10.8% | intergenic (+290/‑55) | PP_2114 → / → PP_2115 | transposase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,414,748 | 0 | C | A | 10.8% | 82.6 / 4.6 | 37 | intergenic (+290/‑55) | PP_2114/PP_2115 | transposase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (14/19); new base A (2/2); total (16/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.88e-01 | |||||||||||
ATTCGCGATGGCGACACTGACTCACCTTTTCGCCCTTACGGCGAGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGCGCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAG > NC_002947/2414600‑2414892 | aTTCGCGATGGCGACACTGACTCACCTTTTCGCCCTTACGGCGAGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGCGCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTccc > 7:73031/1‑149 (MQ=38) gATGGCGACACTGACTCACCTTTTCGCCCTTACGGCGAGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGCGCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAg < 1:140245/148‑1 (MQ=34) gATGGCGACACTGACTCACCTTTTCGCCCTTACGGCGAGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGCGCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAg > 3:215458/1‑148 (MQ=34) gATGGCGACACTGACTCACCTTTTCGCCCTTACGGCGAGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGCGCTCCCATCATCCGGCCCCAGCGCTGCGCACCGGGGTCCCCTCACAACCGCCTTGCTCCCGGCAg > 2:140245/1‑148 (MQ=11) gCGACACTGACTCTCCTTTTCGCCCTTACGGCGAGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGCGCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACcg > 6:167268/1‑149 (MQ=21) aCTCACCTTTTCGCCCTTACGGCGAGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGCGCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAgg > 8:181662/1‑149 (MQ=32) aCTCACCTTTTCGCCCTTACGGCGAGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGCGCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAg < 1:300889/148‑1 (MQ=32) cTCACCTTTTCGCCCTTACGGCGAGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGCGCTCCCATCATCCGGCCCCTGTGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGc < 1:13074/149‑1 (MQ=17) aCCTTTTCGCCCTTACGGCGAGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGCGCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGcccc < 8:271023/149‑1 (MQ=32) aCCTTTTCGCCCTTACGGCGAGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGCGCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGcc < 7:44548/147‑1 (MQ=32) cTTTTCGCCCTTACGGCGAGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGCGCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCa < 5:119298/128‑1 (MQ=32) cTTTTCGCCCTTACGGCGAGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGCGCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCa > 6:119298/1‑128 (MQ=32) tCGCCCTTACGGCGAGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGCGCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCt < 8:162605/148‑1 (MQ=31) ctattgctgtagctattgctgttgctgttgctgttgatcttgatcttgatcttgatcttgatcttgatcttgatcttcgcgacttcaggaggccgaacgcaggccttgcTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCt > 7:303448‑M2/110‑148 (MQ=255) ctgttgatcttgatcttgatcttgatcttgatcttgatcttgatcttcgcgacttcaggaggccgaacgcaggccttgcTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCAcc < 8:303448‑M2/70‑1 (MQ=255) tcttgatcttgatcttgatcttgatcttgatcttcgcgacttcaggaggccgaacgcaggccttgc‑GAAGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCAc < 2:211419‑M2/68‑1 (MQ=255) tcttgatcttgatcttgatcttgatcttgatcttcgcgacttcaggaggccgaacgcaggccttgc‑GAAGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCAc < 8:211186‑M2/68‑1 (MQ=255) tcttgatcttgatcttgatcttgatcttgatcttcgcgacttcaggaggccgaacgcaggccttgc‑GAAGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCAc > 1:211419‑M2/67‑134 (MQ=255) tcttgatcttgatcttgatcttgatcttgatcttcgcgacttcaggaggccgaacgcaggccttgc‑GAAGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCAc > 7:211186‑M2/67‑134 (MQ=255) aaaCGCTGCGCTTCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTTCGCAAGGc < 7:176980/149‑1 (MQ=17) catcatCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGcctc < 5:65522/149‑1 (MQ=32) ccccTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTcgc < 8:73031/149‑1 (MQ=32) cccTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCGCAGCGCTTGACCGGCCTCCAGGCCCGTCCCCTGCCGTCGCCAGGCCGGCGTTCGGCCGCGGGAAGTcgcg > 7:240339/1‑149 (MQ=11) ctCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaagatc < 5:167268/149‑1 (MQ=32) cGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaag < 5:163660/149‑1 (MQ=32) cGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaa < 8:271203/148‑1 (MQ=32) gggTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTg < 6:312479/124‑1 (MQ=32) gggTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTg > 5:312479/1‑124 (MQ=32) tCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGTGCATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaa < 5:101962/137‑1 (MQ=17) cccTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatc > 5:6410/1‑145 (MQ=32) cccTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatc > 5:137733/1‑145 (MQ=32) cTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatc < 3:224241/149‑1 (MQ=32) cGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGcctcc > 7:21132/1‑108 (MQ=32) cGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGcctcc < 8:21132/108‑1 (MQ=32) cgttctgcTCCCGGCAGGACCGCGCCTAAGGCCCCATCCTGGGGCCTAAGCGCTTGACGGGCATCCATGCCCGTCACTTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTGGCTatgatcaagatcaagatcaagatcaagatcaa < 1:19671‑M2/141‑1 (MQ=255) gccttgcTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCGGAAGTCGCGaagatcaagatcaagatcaagatcaagatc > 1:131481‑M2/8‑146 (MQ=255) tgcTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCAGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGaagatcaagatcaagatcaagatcaagatcaagatc > 6:94318‑M2/4‑148 (MQ=255) cTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAACtcgcgaagatcaagatcaagatcaagatcaagatcaagagaacg > 2:19671‑M2/2‑144 (MQ=255) | ATTCGCGATGGCGACACTGACTCACCTTTTCGCCCTTACGGCGAGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGCGCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTCCCCTCACTCCGGCCTTGCTCCCGGCAGGACCGCGCCGAAGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCACCTGCCTTCGCAAGGCCTGCGTTCGGCCTCCTGAAGTCGCGAAGATCAAGATCAAGATCAAGATCAAGATCAAGATCAAG > NC_002947/2414600‑2414892 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |