Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,448,070 T→C 7.7% K1005E (AAG→GAG)  PP_2146 ← SNF2/RAD54 family helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,448,0700TC 7.7% 146.0 / 2.8 53K1005E (AAG→GAG) PP_2146SNF2/RAD54 family helicase
Reads supporting (aligned to +/- strand):  ref base T (23/24);  new base C (2/2);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.63e-01

TAATCAGCTTGAACACGAACACCGGTTTGTCCTGGCCGATGCGGTAGGCACGGTCGGTAGCCTGGTTTTCGCTCGCCGGGTTCCACCAGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTGCGACGGTCGCGAGTGTCGCCGGTCAGCAGGCTGTAGCGGATCTTGCGCTTCTCGAGCTCCTGCTCGATCAGCGCCAGCATCGAGGTGA  >  NC_002947/2447923‑2448213
                                                                                                                                                   |                                                                                                                                                
tAATCAGCTTGAACACGAACACCGGTTTGTCCTGGCCGATGCGGTAGGCACGGTCGGTAGCCGGGTTTTCGCTCGACGGGTTCCACCAGGGGTCGAAGTGTATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTg                                                                                                                                                 <  1:211775/149‑1 (MQ=255)
  aTCAGCTTGAACACGAACACCGGTTTGTCCTGGCCGATGCGGTAGGCACGGTCGGTAGCCTGGTTTTCGCTCGCCGGGTTCCACCAGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAg                                                                                                                                               <  8:17627/149‑1 (MQ=255)
           aacacgaacacCGGTTTGTCCTGGCCGATGCGGTAGGCACGGTCGGTAGCCTGGTTTTCGCTCGCCGGGTTCCACCAGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTACAGCCTACGCCCCCACCCTAGAGGCTGAT‑CAg                                                                                                                                     >  8:120659/1‑149 (MQ=255)
             cacgaacacCGGTTTGTCCTGGCCGATGCGGTAGGCACGGTCGGTAGCCTGGTTTTCGCTCGCCGGGTTCCACCAGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAgg                                                                                                                                    >  8:178254/1‑148 (MQ=255)
             cacgaacacCGGTTTGTCCTGGCCGATGCGGTAGGCACGGTCGGTAGCCTGGTTTTCGCTCGCCGGGTTCCACCAGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTCAAGGCTgggggcgt                                                                                                                                    <  6:235720/149‑8 (MQ=255)
                gaacacCGGTTTGTCCTGGCCGATGCGGTAGGCACGGTCGGTAGCCTGGTTTTCGCTCGCCGGGTTCCACCAGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGa                                                                                                                                   >  1:108695/1‑146 (MQ=255)
                gaacacCGGTTTGTCCTGGCCGATGCGGTAGGCACGGTCGGTAGCCTGGTTTTCGCTCGCCGGGTTCCACCAGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGa                                                                                                                                   <  2:108695/146‑1 (MQ=255)
                gaacacCGGTTTGTCCTGGCCGATGCGGTAGGCACGGTCGGTAGCCTGGTTTTCGCTCGCCGGGTTCCACCAGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAaca                                                                                                                                >  2:8204/1‑149 (MQ=255)
                  acacCGGTTTGTCCTGGCCGATGCGGTAGGCACGGTCGGTAGCCTGGTTTTCGCTCGCCGGGTTCCACCAGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACt                                                                                                                              >  4:6542/1‑149 (MQ=255)
                       ggTTTGTCCTGGCCGATGCGGTAGGCACGGTCGGTAGCCTGGTTTTCGCTCGCCGGGTTCCACCAGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCt                                                                                                                         <  5:239360/149‑1 (MQ=255)
                        gTTTGTCCTGGCCGATGCGGTAGGCACGGTCGGTAGCCTGGTTTTCGCTCGCCGGGTTCCACCAGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTCAAGGCTggg                                                                                                                                         <  2:197625/133‑3 (MQ=255)
                        gTTTGTCCTGGCCGATGCGGTAGGCACGGTCAGTAGCCTGGTTTTCGCTCGCCGGGTTCCACCAGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTCAAGGCTggg                                                                                                                                         >  1:197625/1‑131 (MQ=255)
                         gttGTCCTGGCCGATGCGGTAGGCACGGTCGGTAGCCTGGTTTTCGCTCGCCGGGTTCCACCAGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCttt                                                                                                                       <  8:179793/148‑1 (MQ=255)
                            gTCCTGGCCGATGCGGTAGGCACGGTCGGTAGCCTGGTTTTCGCTCGCCGGGTTCCACCAGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGa                                                                                                                                                >  4:259965/1‑122 (MQ=255)
                            gTCCTGGCCGATGCGGTAGGCACGGTCGGTAGCCTGGTTTTCGCTCGCCGGGTTCCACCAGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGa                                                                                                                                                <  3:259965/122‑1 (MQ=255)
                                tGGCCGATGCGGTAGGCACGGTCGGTAGCCTGGTTTTCGCTCGCCGGGTTCCACCAGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTg                                                                                                                >  6:161367/1‑149 (MQ=255)
                                    cGATGCGGTAGGCACGGTCGGTAGCCTGGTTTTCGCTCGCCGGGTTCCACCAGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTgg                                                                                                            <  7:120659/149‑1 (MQ=255)
                                     gATGCGGTAGGCACGGTCGGTAGCCTGGTTTTCGCTCGCCGGGTTCCACCAGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTgg                                                                                                            >  6:293288/1‑148 (MQ=255)
                                           gTAGGCACGGTCGGTAGCCTGGTTTTCGCTCGCCGGGTTCCACCAGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAActgct                                                                                                     <  4:13571/149‑1 (MQ=255)
                                               gCACGGTCGGTAGCCTGGTTTTCGCTCGCCGGGTTCCACCAGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCAc                                                                                                 >  5:149394/1‑149 (MQ=255)
                                                       ggtAGCCTGGTTTTCGCTCGCCGGGTTCCACCAGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTg                                                                                          <  5:293288/148‑1 (MQ=255)
                                                           gCCTGGTTTTCGCTCGCCGGGTTCCTCCAGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTGCGACg                                                                                     <  6:149394/149‑1 (MQ=255)
                                                            ccTGGTTTTCGCTCGCCGGGTTCCACCAGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTGCGACgg                                                                                    >  5:9889/1‑149 (MQ=255)
                                                            ccTGGTTTTCGCTCGCCGGGTTCCACCAGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACACCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTGCGACgg                                                                                    >  1:180781/1‑149 (MQ=255)
                                                             gtGGGTTTCGCTCGCCGGGTTCCACGAGGGGGCGAAGTGGATCCCGGTGTCGGCCGCCGTCAGGTTCAAGCGTACGCCCCCCGGGTGGAGGCTGAT‑CAGGAACAGTTGGCGTTCACCTTGCTGGAGCTGCGGCACTGGCGTGCGTCGGt                                                                                   >  4:97627/2‑149 (MQ=255)
                                                                          gcCGGGTTCCACCAGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTGCGACGGTCGCGAGTGTCGcc                                                                      <  7:178254/149‑1 (MQ=255)
                                                                             gggTTCCACCAGGGGTCGAAGTGGAGCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTGCGACGGTCGCGAGTGTCGCCGGt                                                                   <  8:23270/149‑1 (MQ=255)
                                                                                ttCCACCAGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTGCGACGGTCGCGAGTGTCGCCGGTcag                                                                >  1:109570/1‑149 (MQ=255)
                                                                                ttCCACCAGGGGTCGAAGTGGATCACGGTGTACGCCGAAGTCAGGTTCAAGCATACGCCCCCAGACTTGAGGCTGAT‑CAGGAACACTTCGATTTAACCTTTATGGAACTGCTGCACTGGCGTGCTACGGTCGCGATTGTAGCCGGTcag                                                                >  1:113213/1‑149 (MQ=255)
                                                                                    accaGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTGCGACGGTCGCGAGTGTCGCCGGTCAGCAgg                                                            <  5:161367/149‑1 (MQ=255)
                                                                                    accaGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTGCGACGGTCGCGAGTGTCGCCGGTCAGCAgg                                                            <  7:100813/149‑1 (MQ=255)
                                                                                    accaGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTGCGACGGTCGCGAGTGTCGCCGGTCAGCAgg                                                            >  3:22421/1‑149 (MQ=255)
                                                                                      caGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTGCGACGGTcgc                                                                                >  1:177/1‑127 (MQ=255)
                                                                                      caGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTGCGACGGTcgc                                                                                <  2:177/127‑1 (MQ=255)
                                                                                      caGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTGCGACGGTCGCGAGTGTCGCCGGTCAGCAGGCt                                                          <  6:9889/149‑1 (MQ=255)
                                                                                           gTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTGCGACGGTCGCGAGTGTCGCCGGTCAGCAGGCTGTAGc                                                     >  2:290406/1‑149 (MQ=255)
                                                                                                     aTCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTGCGACGGTCGCGAGTGTCGCCGGTCAGCAGGCTGTAGCGGATCTTgc                                            <  4:22421/148‑1 (MQ=255)
                                                                                                       cACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTGCGACGGTCGCGAGTGTCGCCGGTCAGCAGGCTGTAGCGGATCTTGCGCt                                         >  7:235117/1‑149 (MQ=255)
                                                                                                              tCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTGCGACGGTCGCGAGTGTCGCCGGTCAGCAGGCTGTAGCGGATCTTGCGCTTCTCGa                                   <  3:21611/148‑1 (MQ=255)
                                                                                                              tCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTGCGACGGTCGCGAGTGTCGCCGGTCAGCAGGCTGTAGCGGATCTTGCGCTTCTCGAg                                  >  6:258050/1‑149 (MQ=255)
                                                                                                                  ccgccgTCAGGTTCAAGCCTACGCCCCCAGCCTCGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTGCGACGGTCGCGAGTGTCGCCGGTCAGCAGGCTGTAGCGGATCTTGCGCTTCTCGAGCTcc                              >  2:314860/1‑149 (MQ=255)
                                                                                                                        tCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTGCGACGGTCGCGAGTGTCGCCGGTCAGCAGGCTGTAGCGGATCTTGCGCTTCTCGAGCTCCTGCTc                         >  6:44602/1‑148 (MQ=255)
                                                                                                                        tCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTGCGACGGTCGCGAGTGTCGCCGGTCAGCAGGCTGTAGCGGATCTTGCGCTTCTCGAGCTCCTGCTc                         >  7:92868/1‑148 (MQ=255)
                                                                                                                                aaGCCTACGCCCCCAGCCTTGAGGCTGATGCAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTGCGACGGTCGCGAGTGTCGCCGGTCAGCAGGCGGTAGCGGATCTTGCGCTTCTCGAGCTCCTGCTCGATCAgcg                 >  1:24320/1‑149 (MQ=255)
                                                                                                                                  gCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTGCGACGGTCGCGAGTGTCGCCGGTCAGCAGGCTGTAGCGGATCTTGCGCTTCTCGAGCTCCTGCTCGATCAgcgc                >  2:231152/1‑147 (MQ=255)
                                                                                                                                       cGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCAGGAACTGCTGCACTGGCGTGCGACGGTCGCGAGTGTCGCCGGTCAGCAGGCTGTAGCGGATCTTGCGCTTGTCGAGCTCCTGCTCGATCAGCGCCAGCATc         <  3:6542/149‑1 (MQ=255)
                                                                                                                                         cccccAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTGCGACGGGCGCGAGTGTCGCCGGTCAGCAGGCTGTAGCGGATCTTGCGCTTCTCGAGCTCCTGCTCGATCAGCGCCAGCATCGa       <  7:147113/149‑1 (MQ=255)
                                                                                                                                          ccccAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTGCGACGGTCGCGAGTGTCGCCGGTCAGCAGGCTGTAGCGGATCTTGCGCTTCTCGAGCTCCTGCTCGATCAGCGCCAGCATCGAg      <  2:109570/149‑1 (MQ=255)
                                                                                                                                          ccacAGCCTTGAGGCTGAT‑CATTAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTTGAGTGCGACTTTCGCGAGTGTCGCCGGTAAGCAGGCTGTATAGTATCTTGCGCTTCTCGAGATCCTGCTCGATCAGCGCCAGCATCGAg      <  2:113213/146‑1 (MQ=255)
                                                                                                                                           cccAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTGCGACGGTCGCGAGTGTCGCCGGTCAGCAGGCTGTAGCGGATCTTGCGCTTCTCGAGCTCCTGCTCGATCAGCGCCAGCATCGAgg     >  2:172456/1‑149 (MQ=255)
                                                                                                                                              aGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTGCGACGGTCGCGAGTGTCGCCGGTCAGCAGGCTGTAGCGGATCTTGCGCTTCTCGAGCTCCTGCTCGATCAGCGCCAGCATCGAGGTGa  <  8:92868/149‑1 (MQ=255)
                                                                                                                                                 cTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTGCGACGGTCGCGAGTGTCGCCGGTCAGCAGGCTGTAGCGGATCTTGCGCTTCTCGAGCTCCTGCt                          <  6:203570/122‑1 (MQ=255)
                                                                                                                                                 cTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTGCGACGGTCGCCAGTGTCGCCGGTCAGCAGGCTGTAGCGGATCTTGCGCTTCTCGAGCTCCTGCt                          >  5:203570/1‑122 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TAATCAGCTTGAACACGAACACCGGTTTGTCCTGGCCGATGCGGTAGGCACGGTCGGTAGCCTGGTTTTCGCTCGCCGGGTTCCACCAGGGGTCGAAGTGGATCACGGTGTCCGCCGCCGTCAGGTTCAAGCCTACGCCCCCAGCCTTGAGGCTGAT‑CAGGAACACTTCGCTTTCACCTTGCTGGAACTGCTGCACTGGCGTGCGACGGTCGCGAGTGTCGCCGGTCAGCAGGCTGTAGCGGATCTTGCGCTTCTCGAGCTCCTGCTCGATCAGCGCCAGCATCGAGGTGA  >  NC_002947/2447923‑2448213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑