Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,456,991 T→A 9.1% L21Q (CTG→CAG)  PP_2150 → thiamine biosynthesis lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,456,9910TA 9.1% 150.9 / 3.9 54L21Q (CTG→CAG) PP_2150thiamine biosynthesis lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (27/22);  new base A (3/2);  total (30/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

CCCGCTCCTACAGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCG  >  NC_002947/2456846‑2457132
                                                                                                                                                  |                                                                                                                                             
cccGCTCCTACAGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTg                                                                                                                                              >  5:146339/1‑147 (MQ=255)
 ccGCTCCTACAGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAg                                                                                                                                           <  6:157029/149‑1 (MQ=255)
 ccGCTCCTACAGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAg                                                                                                                                           >  2:93445/1‑149 (MQ=255)
     tCCTACAGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGAGAACCGGCACCCGAGAACCCCTCCTGTGCGCACAGCCGTACTGTTCATTCTGCTCATACTCACCGCATGCAACAAAGCCCCCACCATGGAccgc                                                                                                                                        >  1:246275/1‑149 (MQ=37)
         aCAGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTggg                                                                                                                                   >  8:169373/1‑149 (MQ=255)
          ccggATTATCGCTCCCCCTTTAGGAGGGGGTTAACCCGCGAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTggg                                                                                                                                   <  5:195302/146‑1 (MQ=255)
          cAGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTggg                                                                                                                                   <  5:99637/148‑1 (MQ=255)
           aGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCAGGCGCTCCAGggtg                                                                                                                                 <  1:40780/149‑1 (MQ=255)
             gATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTgg                                                                                                                                    <  8:139341/144‑1 (MQ=255)
             gATTATCGATGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCACACCCTGGAGCGCCTgg                                                                                                                                    >  7:139341/1‑144 (MQ=255)
                         cccTGTAGGAGCGGGTTTACCCGCGAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTgg                                                                                                                                    >  4:207313/1‑132 (MQ=255)
                         cccTGTAGGAGCGGGTTTACCCGCGAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTgg                                                                                                                                    <  3:207313/132‑1 (MQ=255)
                                             ccGCGAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGggt                                                                                                                                  <  2:124538/114‑1 (MQ=255)
                                             ccGCGAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGggt                                                                                                                                  >  1:124538/1‑114 (MQ=255)
                                              cgcgAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATggg                                                                                                                    >  3:56178/1‑127 (MQ=255)
                                              cgcgAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATggg                                                                                                                    <  4:56178/127‑1 (MQ=255)
                                                     agGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCcgc                                                                                        >  2:147324/1‑148 (MQ=255)
                                                       gCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAg                                                                                     >  3:309423/1‑149 (MQ=255)
                                                       gCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGACCGCGAg                                                                                     >  2:243642/1‑149 (MQ=255)
                                                           aGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGACTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCt                                                                                  >  5:269653/1‑149 (MQ=255)
                                                             cACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCtgg                                                                                <  1:31333/149‑1 (MQ=255)
                                                                cGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCTCCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCtggtgg                                                                             <  1:243642/149‑1 (MQ=255)
                                                                       cGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTg                                                                      >  4:175959/1‑149 (MQ=255)
                                                                           aaaCCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCgg                                                                  <  3:175959/149‑1 (MQ=255)
                                                                               ccTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGcc                                                                >  3:226047/1‑147 (MQ=255)
                                                                                  aCTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTAc                                                                                                          >  1:46994/1‑102 (MQ=255)
                                                                                  aCTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTAc                                                                                                          <  2:46994/102‑1 (MQ=255)
                                                                                   cTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTg                                                          <  6:19448/149‑1 (MQ=255)
                                                                                   cTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATCGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAgg                                                            <  1:93445/147‑1 (MQ=255)
                                                                                      tgCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCa                                                        <  7:169373/148‑1 (MQ=255)
                                                                                       gcgcACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGt                                                                                                >  6:247107/1‑107 (MQ=255)
                                                                                       gcgcACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGt                                                                                                <  5:247107/107‑1 (MQ=255)
                                                                                            caGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCg                                                 <  4:309423/149‑1 (MQ=255)
                                                                                                 cTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCCCCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGCTGGCGGTgg                                             >  4:287453/1‑148 (MQ=255)
                                                                                                   aCTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGaaa                                          >  1:36916/1‑149 (MQ=255)
                                                                                                    cTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGaaa                                          <  6:146339/148‑1 (MQ=255)
                                                                                                    cTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAAc                                         >  6:133700/1‑149 (MQ=255)
                                                                                                             ctgctgCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGc                                 <  1:147324/148‑1 (MQ=255)
                                                                                                                   ctACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACAt                          >  7:234023/1‑149 (MQ=255)
                                                                                                                       tCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACa                           >  4:183296/1‑144 (MQ=255)
                                                                                                                       tCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACa                           <  3:183296/144‑1 (MQ=255)
                                                                                                                         aCCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGacca                    >  7:201404/1‑149 (MQ=255)
                                                                                                                         aCCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGacc                     <  3:292959/148‑1 (MQ=255)
                                                                                                                                gCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGGAGATGGCGGt                                               <  4:298149/115‑1 (MQ=255)
                                                                                                                                gCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGt                                               >  3:298149/1‑115 (MQ=255)
                                                                                                                                  aaCCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTc           >  4:81407/1‑149 (MQ=255)
                                                                                                                                        ggCACCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCtc     >  3:287573/1‑148 (MQ=255)
                                                                                                                                         ggaccACCCAGGCGCTCCAGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTAc    >  1:16211/4‑149 (MQ=255)
                                                                                                                                           cccACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACcg  >  6:300515/1‑149 (MQ=255)
                                                                                                                                           cccACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACcg  <  3:81407/149‑1 (MQ=255)
                                                                                                                                           cccACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGAGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACcg  >  3:235916/1‑149 (MQ=255)
                                                                                                                                           accACCCAGGCGCTCCAGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCt                                   >  2:35859/2‑116 (MQ=255)
                                                                                                                                           accACCCAGGCGCTCCAGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCt                                   <  1:35859/115‑1 (MQ=255)
                                                                                                                                           accACCCAGGCGCTCCAGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACcg  >  4:59601/2‑149 (MQ=255)
                                                                                                                                                  tGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATc                         <  1:61465/119‑1 (MQ=255)
                                                                                                                                                  tGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATc                         >  2:61465/1‑119 (MQ=255)
                                                                                                                                                  |                                                                                                                                             
CCCGCTCCTACAGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCG  >  NC_002947/2456846‑2457132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑