Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,456,997 G→T 9.4% R23L (CGC→CTC)  PP_2150 → thiamine biosynthesis lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,456,9970GT 9.4% 156.9 / 6.9 53R23L (CGC→CTC) PP_2150thiamine biosynthesis lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (25/23);  new base T (3/2);  total (28/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.63e-01

TCCTACAGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCGCGGCGATT  >  NC_002947/2456851‑2457140
                                                                                                                                                   |                                                                                                                                               
tCCTACAGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGAGAACCGGCACCCGAGAACCCCTCCTGTGCGCACAGCCGTACTGTTCATTCTGCTCATACTCACCGCATGCAACAAAGCCCCCACCATGGAccgc                                                                                                                                                >  1:246275/1‑149 (MQ=37)
    aCAGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTggg                                                                                                                                           >  8:169373/1‑149 (MQ=255)
     ccggATTATCGCTCCCCCTTTAGGAGGGGGTTAACCCGCGAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTggg                                                                                                                                           <  5:195302/146‑1 (MQ=255)
     cAGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTggg                                                                                                                                           <  5:99637/148‑1 (MQ=255)
      aGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCAGGCGCTCCAGggtg                                                                                                                                         <  1:40780/149‑1 (MQ=255)
        gATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTgg                                                                                                                                            <  8:139341/144‑1 (MQ=255)
        gATTATCGATGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCACACCCTGGAGCGCCTgg                                                                                                                                            >  7:139341/1‑144 (MQ=255)
                    cccTGTAGGAGCGGGTTTACCCGCGAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTgg                                                                                                                                            >  4:207313/1‑132 (MQ=255)
                    cccTGTAGGAGCGGGTTTACCCGCGAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTgg                                                                                                                                            <  3:207313/132‑1 (MQ=255)
                                        ccGCGAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGggt                                                                                                                                          >  1:124538/1‑114 (MQ=255)
                                        ccGCGAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGggt                                                                                                                                          <  2:124538/114‑1 (MQ=255)
                                         cgcgAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATggg                                                                                                                            >  3:56178/1‑127 (MQ=255)
                                         cgcgAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATggg                                                                                                                            <  4:56178/127‑1 (MQ=255)
                                                agGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCcgc                                                                                                >  2:147324/1‑148 (MQ=255)
                                                  gCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAg                                                                                             >  3:309423/1‑149 (MQ=255)
                                                  gCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGACCGCGAg                                                                                             >  2:243642/1‑149 (MQ=255)
                                                      aGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGACTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCt                                                                                          >  5:269653/1‑149 (MQ=255)
                                                        cACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCtgg                                                                                        <  1:31333/149‑1 (MQ=255)
                                                           cGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCTCCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCtggtgg                                                                                     <  1:243642/149‑1 (MQ=255)
                                                                  cGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTg                                                                              >  4:175959/1‑149 (MQ=255)
                                                                      aaaCCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCgg                                                                          <  3:175959/149‑1 (MQ=255)
                                                                          ccTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGcc                                                                        >  3:226047/1‑147 (MQ=255)
                                                                             aCTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTAc                                                                                                                  >  1:46994/1‑102 (MQ=255)
                                                                             aCTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTAc                                                                                                                  <  2:46994/102‑1 (MQ=255)
                                                                              cTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTg                                                                  <  6:19448/149‑1 (MQ=255)
                                                                              cTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATCGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAgg                                                                    <  1:93445/147‑1 (MQ=255)
                                                                                 tgCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCa                                                                <  7:169373/148‑1 (MQ=255)
                                                                                  gcgcACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGt                                                                                                        >  6:247107/1‑107 (MQ=255)
                                                                                  gcgcACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGt                                                                                                        <  5:247107/107‑1 (MQ=255)
                                                                                       caGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCg                                                         <  4:309423/149‑1 (MQ=255)
                                                                                            cTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCCCCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGCTGGCGGTgg                                                     >  4:287453/1‑148 (MQ=255)
                                                                                              aCTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGaaa                                                  >  1:36916/1‑149 (MQ=255)
                                                                                               cTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGaaa                                                  <  6:146339/148‑1 (MQ=255)
                                                                                               cTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAAc                                                 >  6:133700/1‑149 (MQ=255)
                                                                                                        ctgctgCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGc                                         <  1:147324/148‑1 (MQ=255)
                                                                                                              ctACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACAt                                  >  7:234023/1‑149 (MQ=255)
                                                                                                                  tCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACa                                   >  4:183296/1‑144 (MQ=255)
                                                                                                                  tCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACa                                   <  3:183296/144‑1 (MQ=255)
                                                                                                                    aCCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGacca                            >  7:201404/1‑149 (MQ=255)
                                                                                                                    aCCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGacc                             <  3:292959/148‑1 (MQ=255)
                                                                                                                           gCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGGAGATGGCGGt                                                       <  4:298149/115‑1 (MQ=255)
                                                                                                                           gCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGt                                                       >  3:298149/1‑115 (MQ=255)
                                                                                                                             aaCCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTc                   >  4:81407/1‑149 (MQ=255)
                                                                                                                                   ggCACCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCtc             >  3:287573/1‑148 (MQ=255)
                                                                                                                                    ggaccACCCAGGCGCTCCAGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTAc            >  1:16211/4‑149 (MQ=255)
                                                                                                                                      cccACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACcg          <  3:81407/149‑1 (MQ=255)
                                                                                                                                      cccACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACcg          >  6:300515/1‑149 (MQ=255)
                                                                                                                                      cccACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGAGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACcg          >  3:235916/1‑149 (MQ=255)
                                                                                                                                      accACCCAGGCGCTCCAGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCt                                           >  2:35859/2‑116 (MQ=255)
                                                                                                                                      accACCCAGGCGCTCCAGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCt                                           <  1:35859/115‑1 (MQ=255)
                                                                                                                                      accACCCAGGCGCTCCAGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACcg          >  4:59601/2‑149 (MQ=255)
                                                                                                                                             tGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATc                                 >  2:61465/1‑119 (MQ=255)
                                                                                                                                             tGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATc                                 <  1:61465/119‑1 (MQ=255)
                                                                                                                                               gAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCGCGGCGAtt  <  6:269653/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
TCCTACAGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCC‑AGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCGCGGCGATT  >  NC_002947/2456851‑2457140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑