Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,555,170	A→G	6.1%	Q384R (CAG→CGG)	PP_2240 →	ABC transporter permease/ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,555,170	0	A	G	6.1%	193.3 / 2.7	65	Q384R (CAG→CGG)	PP_2240	ABC transporter permease/ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (29/32);  new base G (2/2);  total (31/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

TTGTGTTTCCGTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCCGGACGGACATGCCCTTATC  >  NC_002947/2555029‑2555318                                                                                                                                               |                                                                                                                                                     ttgtGTTTCCGTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACcgc                                                                                                                                               >  8:179519/1‑149 (MQ=255)   gtgtTTCCGTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTCCcgcgc                                                                                                                                             >  3:259951/1‑149 (MQ=255)   gtgtTTCCGTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACcgcg                                                                                                                                              <  1:139380/148‑1 (MQ=255)     gtTTCCGTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCGGTACtgcgcg                                                                                                                                            >  2:40696/1‑148 (MQ=255)      tttCCGTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCGGTACTGCGCGaa                                                                                                                                          >  4:111762/1‑148 (MQ=255)      tttCCGTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGAc                                                                                                                                          >  5:245439/1‑149 (MQ=255)          cGTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCGGTACTGCGCGaacgca                                                                                                                                      <  3:111762/149‑6 (MQ=255)          cGTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCtt                                                                                                                                      >  4:38551/1‑149 (MQ=255)           gTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCGGTACTGCGCGaacgcat                                                                                                                                     <  1:40696/149‑7 (MQ=255)                 tcttcAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGtc                                                                                                                               >  1:283426/1‑149 (MQ=255)                  ctcccgccgccgcGCTGCTTCCGTGCGGCGATCAAGCTGGCCGCCGTCACCCACCCTTCCCAGCCCTTTCGGCAGGT‑GCAGGATGCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGtct                                                                                                                              <  4:259951/142‑1 (MQ=255)                     cAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCAc                                                                                                                           >  3:250892/1‑149 (MQ=255)                          gccgcGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCg                                                                                                                                 >  3:262538/1‑138 (MQ=255)                          gccgcGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCg                                                                                                                                 <  4:262538/138‑1 (MQ=255)                           ccgcGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCtt                                                                                                                     >  7:92489/1‑149 (MQ=255)                            cgcgCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCa                                                                                                                            >  6:175718/1‑141 (MQ=255)                            cgcgCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCa                                                                                                                            <  5:175718/141‑1 (MQ=255)                            cgcgCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCtt                                                                                                                     >  3:177197/1‑148 (MQ=255)                                         tGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAAcc                                                                                                       <  7:56576/149‑1 (MQ=255)                                           gggCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGAt                                                                                                              <  1:283937/140‑1 (MQ=255)                                           gggCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGAt                                                                                                              >  2:283937/1‑140 (MQ=255)                                            ggCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACcagc                                                                                                    <  3:135947/149‑1 (MQ=255)                                            ggCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACcagc                                                                                                    <  6:245439/149‑1 (MQ=255)                                               gATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGTGGCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACcagcag                                                                                                  >  1:75674/1‑149 (MQ=255)                                                aTCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGc                                                                                                <  4:177197/149‑1 (MQ=255)                                                   aaGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTCCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAg                                                                                             >  6:293927/1‑149 (MQ=255)                                                     gCTGGGCGACGTCATGCAAACTT‑CCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGAccc                                                                                                                                        >  3:129080/1‑103 (MQ=255)                                                     gCTGGGCGACGTCATGCAAACTT‑CCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGAccc                                                                                                                                        <  4:129080/103‑1 (MQ=255)                                                       tGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGc                                                                                                >  6:269353/1‑142 (MQ=255)                                                       tGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGc                                                                                                <  5:269353/142‑1 (MQ=255)                                                            gACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACcgcgc                                                                                                                                             >  3:180259/1‑92 (MQ=255)                                                            gACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACcgcgc                                                                                                                                             <  4:180259/92‑1 (MQ=255)                                                             aCGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCcg                                                                                   <  3:38551/149‑1 (MQ=255)                                                                         cTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCaccacc                                                                       <  4:250892/149‑1 (MQ=255)                                                                         cTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCaccacc                                                                       <  7:179519/149‑1 (MQ=255)                                                                            cccAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACAGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCg                                                                      >  6:52608/1‑147 (MQ=255)                                                                             ccAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAg                                                                   <  1:210194/149‑1 (MQ=255)                                                                              cAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGa                                                                  >  4:87340/1‑149 (MQ=255)                                                                              cAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGa                                                                  >  6:157765/1‑149 (MQ=255)                                                                                    tttGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGgc                                                            >  4:99387/1‑149 (MQ=255)                                                                                      tGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGgc                                                            <  7:227896/147‑1 (MQ=255)                                                                                                gCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGc                                                 <  3:87340/149‑1 (MQ=255)                                                                                                 cAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTg                                                  >  6:183443/1‑147 (MQ=255)                                                                                                  aGGATTCTCTGTCGTTCTCCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAg                                               <  5:293927/149‑1 (MQ=255)                                                                                                           tGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACgg                                        <  5:157765/147‑1 (MQ=255)                                                                                                           tGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGa                                      >  4:28455/1‑149 (MQ=255)                                                                                                            gTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGAt                                     >  1:319231/1‑149 (MQ=255)                                                                                                             tCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGAtt                                    <  2:241468/149‑1 (MQ=255)                                                                                                                   ttcCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGt                              >  2:84404/1‑149 (MQ=255)                                                                                                                     ccGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAgg                               <  5:52608/146‑1 (MQ=255)                                                                                                                       gTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAAGCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTg                                                  <  8:101870/125‑1 (MQ=255)                                                                                                                       gTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTg                                                  >  7:101870/1‑125 (MQ=255)                                                                                                                        tGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGtgctgc                         <  5:291324/149‑1 (MQ=255)                                                                                                                        tGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGtgctgc                         <  2:75674/149‑1 (MQ=255)                                                                                                                          aGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTgcg                        <  2:88699/148‑1 (MQ=255)                                                                                                                              gTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGc                                                 <  4:226518/119‑1 (MQ=255)                                                                                                                              gTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGc                                                 >  3:226518/1‑119 (MQ=255)                                                                                                                               tACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACgg                                        >  5:41801/1‑127 (MQ=255)                                                                                                                               tACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACgg                                        <  6:41801/127‑1 (MQ=255)                                                                                                                               tACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCcgga                  <  5:183443/149‑1 (MQ=255)                                                                                                                                  gATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGTTGCGCCcggacgg               <  8:92489/149‑1 (MQ=255)                                                                                                                                  gATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCcggacgg               <  3:28455/149‑1 (MQ=255)                                                                                                                                  gATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGACcggacgg               >  5:319590/1‑149 (MQ=255)                                                                                                                                    tGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCCggacggac             <  2:319231/149‑1 (MQ=255)                                                                                                                                             gcAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCCTAGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCCGGACGGACATCCCCtt     >  7:91451/1‑148 (MQ=255)                                                                                                                                               aGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCCGGACGGACATGCCCTTATc  >  7:266384/1‑149 (MQ=255)                                                                                                                                               |                                                                                                                                                     TTGTGTTTCCGTTCATTCTTCAGGCGCCGCGCTTCTTCAGTGGGGCGATCAAGCTGGGCGACGTCATGCAAACTTCCCAGGCCTTTGGGCAGGT‑GCAGGATTCTCTGTCGTTCTTCCGTGAGTCGTACGATGCGTTCGCGCAGTACCGCGCGACCCTTGACCGTCTCACCGGCTTTCTCGATGCCAACCAGCAGGCAAGTGCGTTGCCGCGCGTCACCACCGAAGACCAGGCGCATGCGCTGCAGATCACGGGATTGCAGGTGCTGCGCCCGGACGGACATGCCCTTATC  >  NC_002947/2555029‑2555318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑