Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,628,386 A→C 10.8% A16A (GCA→GCC)  clpP → ATP‑dependent Clp protease proteolytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,628,3860AC10.8% 177.9 / 13.6 65A16A (GCA→GCC) clpPATP‑dependent Clp protease proteolytic subunit
Reads supporting (aligned to +/- strand):  ref base A (29/29);  new base C (4/3);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01

AGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCTCGTCGTTGCGCAA  >  NC_002947/2628243‑2628527
                                                                                                                                               |                                                                                                                                             
aGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAggcg                                                                                                                                           <  5:12122/148‑1 (MQ=255)
    aCGGTCTTCTCTCGTATGAAACCTCCACAAGCCAGGCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGTCTATTTGTGAGTGAGCTCAGGACATGTCCAGCAATTCTTATATTCAGGAGAGCTCTGACATCCAGGCCGCAGGCGGCCTg                                                                                                                                      <  4:237615/149‑1 (MQ=255)
     cGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCCTGCGGCCTgg                                                                                                                                     <  7:29415/149‑1 (MQ=255)
       gTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCCTGCGGCCTggat                                                                                                                                   <  5:158213/149‑3 (MQ=255)
       gTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTc                                                                                                                                   <  6:153618/149‑1 (MQ=255)
            ctctcGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGAt                                                                                                                              >  8:86332/1‑149 (MQ=255)
            ctctcGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGAt                                                                                                                              >  2:85684/1‑149 (MQ=255)
               tcGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATg                                                                                                                             <  7:86332/147‑1 (MQ=255)
                 gTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTa                                                                                                                         >  5:9159/1‑149 (MQ=255)
                  tAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTAt                                                                                                                        <  8:44483/149‑1 (MQ=255)
                      aaaCCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAg                                                                                                                    <  7:46558/149‑1 (MQ=255)
                           tCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTc                                                                                                               >  7:213732/1‑149 (MQ=255)
                             cacaAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTcc                                                                                                              <  3:103865/148‑1 (MQ=255)
                                aaGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGccc                                                                                                          >  5:172841/1‑149 (MQ=255)
                                     agccTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCa                                                                                                                  >  7:13126/1‑136 (MQ=255)
                                     agccTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCa                                                                                                                  <  8:13126/136‑1 (MQ=255)
                                     agccTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGc                                                                                                     >  7:194592/1‑149 (MQ=255)
                                           cGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAAc                                                                                                 <  4:122142/147‑1 (MQ=255)
                                                    ttGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGa                                                                                       >  5:185048/1‑148 (MQ=255)
                                                      gtgCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACa                                                                                     >  5:264504/1‑148 (MQ=255)
                                                      gtgCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACa                                                                                     <  3:62970/148‑1 (MQ=255)
                                                          gTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTa                                                                                 >  4:1443/1‑148 (MQ=255)
                                                            aTTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTc                                                                              >  7:70875/1‑149 (MQ=255)
                                                            aTTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTc                                                                              >  7:284386/1‑149 (MQ=255)
                                                                aaGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGc                                                                                                     >  7:31727/1‑122 (MQ=255)
                                                                aaGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGc                                                                                                     <  8:31727/122‑1 (MQ=255)
                                                                   ccATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTg                                                                       >  6:305852/1‑149 (MQ=255)
                                                                         cTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGaa                                                                  <  3:1443/148‑1 (MQ=255)
                                                                         cTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAg                                                                 <  8:213732/149‑1 (MQ=255)
                                                                         cTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAg                                                                 <  6:264504/149‑1 (MQ=255)
                                                                                      aGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCt                                                    >  5:137173/1‑149 (MQ=255)
                                                                                          cAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCt                                                >  6:51890/1‑149 (MQ=255)
                                                                                           aGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTg                                               >  4:248592/1‑149 (MQ=255)
                                                                                                 tGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGc                                         <  6:9159/149‑1 (MQ=255)
                                                                                                  gTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGcc                                        >  5:246742/1‑149 (MQ=255)
                                                                                                   tCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGccc                                       >  1:323963/1‑149 (MQ=255)
                                                                                                   tCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGccc                                       <  1:197900/149‑1 (MQ=255)
                                                                                                     ccGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCgg                                     <  2:260478/149‑1 (MQ=255)
                                                                                                     ccGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGACTGGTCCCGATGGTAATCGAGCAGTCCGCCCGTGGCGAAAGCGATTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCAAGTTTGGcaccg                                     >  4:156795/1‑145 (MQ=255)
                                                                                                      cGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTc                                                  <  6:168027/135‑1 (MQ=255)
                                                                                                      cGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTc                                                  >  5:168027/1‑135 (MQ=255)
                                                                                                       gCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTa                                   <  8:284386/149‑1 (MQ=255)
                                                                                                       gCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTa                                   <  6:137173/149‑1 (MQ=255)
                                                                                                         aaTTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTaga                                 <  3:156795/149‑1 (MQ=255)
                                                                                                          aTTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACAt                                                                                    >  2:251965/1‑97 (MQ=255)
                                                                                                          aTTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACAt                                                                                    <  1:251965/97‑1 (MQ=255)
                                                                                                          aTTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTagag                                <  5:305852/149‑1 (MQ=255)
                                                                                                             cTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGa                              <  6:172841/148‑1 (MQ=255)
                                                                                                                atatTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACt                            <  5:51890/147‑1 (MQ=255)
                                                                                                                atatTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACt                            <  6:185048/147‑1 (MQ=255)
                                                                                                                   ttCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATg                       >  3:191593/1‑149 (MQ=255)
                                                                                                                      agcagAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTa                                   <  6:324014/134‑1 (MQ=255)
                                                                                                                      agcagAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTa                                   >  5:324014/1‑134 (MQ=255)
                                                                                                                        cagAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCaa                  >  2:141235/1‑149 (MQ=255)
                                                                                                                            gCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCtc              <  6:246742/149‑1 (MQ=255)
                                                                                                                              tctGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCtcgt            >  6:242760/1‑149 (MQ=255)
                                                                                                                                tcgggaccAGGCCGCCTGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCtcgtcg          >  7:151196/7‑149 (MQ=255)
                                                                                                                                  aCATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCTCGTCGtt        >  8:201401/1‑149 (MQ=255)
                                                                                                                                    gaccAGGCCGCCTGCGGCCTGGTCCCGATGGTTTTCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCTCGTCGTTgc      >  1:188793/3‑149 (MQ=255)
                                                                                                                                    aTCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCTCGTCGTTgc      >  2:276587/1‑149 (MQ=255)
                                                                                                                                     tCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCTCGTCGTTgcg     <  8:70875/149‑1 (MQ=255)
                                                                                                                                       cAGGCCGCCTGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGc                                                             >  7:5145/1‑91 (MQ=255)
                                                                                                                                       cAGGCCGCCTGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGc                                                             <  8:5145/91‑1 (MQ=255)
                                                                                                                                        aGGCCGCCTGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACAACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCTCGTCGTTGCGCaa  >  4:301731/1‑149 (MQ=255)
                                                                                                                                        aGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCTCGTCGTTGCGCa   >  3:186249/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                             
AGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCTCGTCGTTGCGCAA  >  NC_002947/2628243‑2628527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑