Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,640,007 A→T 7.4% V217D (GTC→GAC)  PP_2310 ← methyl‑accepting chemotaxis transducer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,640,0070AT 7.4% 163.1 / 3.4 54V217D (GTC→GAC) PP_2310methyl‑accepting chemotaxis transducer
Reads supporting (aligned to +/- strand):  ref base A (27/23);  new base T (2/2);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

TGTGGTGCACGGTCGCCGACATTTCGTTGATGGCAGTGGCGGCCTGGTCGGTTTCACTCTGCTGGCCGAGCATGCCATGGCGTACGTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCACTGGCTAGCGGTACGCGGGCCGACAGGTCGCCGGTTTTTTCCACGTGCAGCATCACGTCCTTGAGGGTGTTGA  >  NC_002947/2639861‑2640154
                                                                                                                                                  |                                                                                                                                                   
tgtgGTGCACGGTCGCCGACATTTCGTTGATGGCAGTGGCGGCCTGGTCGGTTTCACTCTGCTGGCCGAGCATGCCATGGCGTACGTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACg                                                                                                                                                   <  1:214461/149‑1 (MQ=255)
      gCACGGTCGCCGACATTTCGTTGATGGCAGTGGCGGCCTGGTCGGTTTCACTCTGCTGGCCGAGCATGCCATGGCGTACGTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGTCGGTGCt                                                                                                                                              >  4:147571/1‑148 (MQ=255)
      gCACGGTCGCCGACATTTCGTTGATGGCAGTGGCGGCCTGGTCGGTTTCACTCTGCTGGCCGAGCATGCCATGGCGTACGTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGTCGGTGCt                                                                                                                                              >  4:142707/1‑148 (MQ=255)
      gCACGGTCGCCGACATTTCGTTGATGGCAGTGGCGGCCTGGTCGGTTTCACTCTGCTGGCCGAGCATGCCATGGCGTACGTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCt                                                                                                                                              >  3:255677/1‑148 (MQ=255)
        aCGGTCGCCGACATTTCGTTGATGGCAGTGGCGGCCTGGTCGGTTTCACTCTGCTGGCCGAGCATGCCATGGCGTACGTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGAc                                                                                                                                           >  8:27345/1‑149 (MQ=255)
           gTCGCCGACATTTCGTTGATGGCAGTGGCGGCCTGGTCGGTTTCACTCTGCTGGCCGAGCATGCCATGGCGTACGTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCAc                                                                                                                                        <  4:235018/149‑1 (MQ=255)
            tCGCCGACATTTCGTTGATGGCAGTGGCGGCCTGGTCGGTTTCACTCTGCTGGCCGAGCATGCCATGGCGTACGTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCAcg                                                                                                                                       >  4:22395/1‑149 (MQ=255)
                  aCATTTCGTTGATGGCAGTGGCGGCCTGGTCGGTTTCACTCTGCTGGCCGAGCATGCCATGGCGTACGTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGa                                                                                                                                 >  4:226274/1‑149 (MQ=255)
                    aTTTCGTTGATGGCAGTGGCGGCCTGGTCGGTTTCACTCTGCTGGCCGAGCATGCCATGGCGTACGTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATa                                                                                                                               <  4:255677/149‑1 (MQ=255)
                        cGTTGATGGCAGTGGCGGCCTGGTCGGTTTCACTCTGCTGGCCGAGCATGCCATGGCGTACGTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGt                                                                                                                            <  2:107990/148‑1 (MQ=255)
                         gTTGATGGCAGTGGCGGCCTGGTCGGTTTCACTCTGCTGGCCGAGCATGCCATGGCGTACGTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTgg                                                                                                                          <  7:27345/149‑1 (MQ=255)
                         gTTGATGGCAGTGGCGGCCTGGTCGGTTTCACTCTGCTGGCCGAGCATGCCATGGCGTACGTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTg                                                                                                                           >  5:268791/1‑148 (MQ=255)
                          ttGATGGCAGTGGCGGCCTGGTCGGTTTCACTCTGCTGGCCGAGCATGCCATGGCGTACGTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGa                                                                                                                         <  8:178822/149‑1 (MQ=255)
                                gCAGTGGCGGCCTGGTCGGTTTCACTCTGCTGGCCGAGCATGCCATGGCGTACGTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCa                                                                                                                    <  7:126872/148‑1 (MQ=255)
                                  aGTGGCGGCCTGGTCGGTTTCACTCTGCTGGCCGAGCATGCCATGGCGTACGTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATgg                                                                                                                 >  5:273624/1‑149 (MQ=255)
                                            tGGTCGGTTTCACTCTGCTGGCCGAGCATGCCATGGCGTACGTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGc                                                                                                       >  6:211561/1‑149 (MQ=255)
                                              gTCGGTTTCACTCTGCTGGCCGAGCATGCCATGGCGTACGTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCt                                                                                                      <  5:211561/148‑1 (MQ=255)
                                               tCGGTTTCACTCTGCTGGCCGAGCATGCCATGGCGTACGTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTcc                                                                                                    >  2:217823/1‑149 (MQ=255)
                                                         tctGCTGGCCGAGCATGCCATGGCGTACGTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCt                                                                                                                                              <  7:90033/97‑1 (MQ=255)
                                                         tctGCTGGCCGAGCATGCCATGGCGTACGTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCt                                                                                                                                              >  8:90033/1‑97 (MQ=255)
                                                           tgctgGCCGAGCATGCCATGGCGTACGTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGcc                                                                                        >  7:233083/1‑149 (MQ=255)
                                                                      cATGCCATGGCGTACGTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGc                                                                             >  5:22292/1‑149 (MQ=255)
                                                                       aTGCCATGGCGTACGTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGcc                                                                            >  7:120500/1‑149 (MQ=255)
                                                                         gCCATGGCGTACGTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCAc                                                                          >  4:239837/1‑149 (MQ=255)
                                                                         gCCATGGCGTACGTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCAc                                                                          >  8:271245/1‑149 (MQ=255)
                                                                         gCCATGGCGTACGTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCAc                                                                          >  5:121621/1‑149 (MQ=255)
                                                                                 gTACGTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCACTGGCTAGc                                                                  >  4:110521/1‑149 (MQ=255)
                                                                                  tACGTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCACTGGCTAGCg                                                                 >  5:284478/1‑149 (MQ=255)
                                                                                     gTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCACTGGCTAGCGGTa                                                              <  7:271245/149‑1 (MQ=255)
                                                                                       cGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCACTGGCTAGCGGTAcg                                                            >  5:139574/1‑149 (MQ=255)
                                                                                          tCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGTGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCACTGGCTAGCGGTACGCgg                                                         >  5:55658/1‑149 (MQ=255)
                                                                                          tCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCACTGGCTAGCGGTACGCgg                                                         >  4:280409/1‑149 (MQ=255)
                                                                                          tCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCACTGGCTAGCGGTACGCgg                                                         >  4:22935/1‑149 (MQ=255)
                                                                                                  gcggcCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGTCGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCACTGGCTAGCGGTACGCGGGCCGACa                                                  <  3:142707/148‑1 (MQ=255)
                                                                                                  gcggcCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGTCGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCACTGGCTAGCGGTACGCGGGCCGACa                                                  <  3:147571/148‑1 (MQ=255)
                                                                                                     gcCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCACTGGCTAGCGGTACGCGGGCCGACAGGTc                                              >  4:47615/1‑149 (MQ=255)
                                                                                                       cAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCACTGGCTAGCGGTACGCGGGCCGACAGGTCGc                                            <  3:280409/149‑1 (MQ=255)
                                                                                                          gCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCACTGGCTAGCGGTACGCGGGCCGACAGGTCGCCgg                                         >  1:121405/1‑149 (MQ=255)
                                                                                                            gTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAg                                                                                                         >  8:186769/1‑83 (MQ=255)
                                                                                                            gTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCACTGGCTAGCGGTACGCGGGCCGACAGGTCGCCGGtt                                       <  3:22395/149‑1 (MQ=255)
                                                                                                               cAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGTGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCACTGGCTAGCGGTACGCGGGCCGACAGGTCGCCGGttttt                                    <  6:55658/149‑1 (MQ=255)
                                                                                                                aGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCACTGGCTAGCGGTACGCGGGCCGACAGGTCGCCGGtttttt                                   <  6:284478/149‑1 (MQ=255)
                                                                                                                    cccGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCACTGGCTAGCGGTACGCGGGCCGACAGGTCGCCGGTTTTTTCCAc                               >  7:61910/1‑149 (MQ=255)
                                                                                                                    cccGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCACTGGCTAGCGGTACGCGGGCCGACAGGTCGCCGGTTTTTTCCAc                               <  8:233083/149‑1 (MQ=255)
                                                                                                                    cccGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCACTGGCTAGCGGTACGCGGGCCGACAGGTCGCCGGTTTTTTCCAc                               <  1:217823/149‑1 (MQ=255)
                                                                                                                        aGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCACTGGCTAGCGGTACGCGGGCCGACAGGTCGCCGGTTTTTTCCACGTgc                           <  3:226274/149‑1 (MQ=255)
                                                                                                                            cAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCACTGGCTAGCGGTACGCGGGCCGACAGGTCGCCGGTTTTTTCCACGTgcagc                        <  6:273624/148‑1 (MQ=255)
                                                                                                                                      gcgcTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCACTGGCTAGCGGTACGCGGGCCGACAGGTCGCCGGTTTTTTCCACGTGCAGCATCACGTCCtt             >  7:6466/1‑149 (MQ=255)
                                                                                                                                         cTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCACTGGCTAGCGGTACGCGGGCCGACAGGTCGCCGGTTTTTTCCACGTGCAGCATCACGTCCTTGAg          <  6:17349/149‑1 (MQ=255)
                                                                                                                                          tgtgGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCACTGGCTAGCGGTACGCGGGCCGACAGGTCGCCGGTTTTTTCCACGTGCAGCATCACGTCCTTGAgg         >  1:6631/1‑149 (MQ=255)
                                                                                                                                              ggCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCACTGGCTAGCGGTACGCGGGCCGACAGGTCGCCGGTTTTTTCCACGTGCAGCATCACGTCCTTGAGGgtg      <  8:6466/148‑1 (MQ=255)
                                                                                                                                               gCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCACTGGCTAGCGGTACGCGGGCCGACAGGTCGCCGGTTTTTTCCACGTGCAGCATCACGTCCTTGAGGGTGtt    <  6:22292/149‑1 (MQ=255)
                                                                                                                                               gCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCACTGGCTAGCGGTACGCGGGCCGACAGGTCGCCGGTTTTTTCCACGTGCAGCATCACGTCCTTGAGGGTGtt    <  8:61910/149‑1 (MQ=255)
                                                                                                                                                 gACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCACTGGCTAGCGGTACGCTGGCCGACAGGTCGCCGGTTTTTTCCACGTGCAGCATCACGTCCTTGAGGGTGTTGa  <  3:22935/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TGTGGTGCACGGTCGCCGACATTTCGTTGATGGCAGTGGCGGCCTGGTCGGTTTCACTCTGCTGGCCGAGCATGCCATGGCGTACGTCGCTCATGCTGGCGGCCAGGCGTGCAGCGCCCGAGTCCAGCTGTGCTGCGCTGTGGGCGACGGTGCTGACCACGCGGTGATAGGTGGACTGCATGGCGTTGAAGGCTCCGGCCATCTGGCCGACCTCGTCGCCACTGGCTAGCGGTACGCGGGCCGACAGGTCGCCGGTTTTTTCCACGTGCAGCATCACGTCCTTGAGGGTGTTGA  >  NC_002947/2639861‑2640154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑