| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,730,895 | C→G | 7.3% | P78A (CCG→GCG) | polB → | DNA polymerase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,730,895 | 0 | C | G | 7.3% | 169.2 / 4.5 | 55 | P78A (CCG→GCG) | polB | DNA polymerase II |
| Reads supporting (aligned to +/- strand): ref base C (26/25); new base G (2/2); total (28/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.57e-01 | |||||||||||
CGATGCCGGGCCGCGACTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGGCCGCCGGAGCGTTACCTCATGGAGCGCTTCATCACGGCACCGGTG > NC_002947/2730747‑2731038 | cGATGCCGGGCCGCGACTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑Gcc > 6:330439/1‑149 (MQ=255)cGATGCCGGGCCGCGACTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑Gcc < 6:75604/149‑1 (MQ=255) ggCCGCGACTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTc > 2:33239/1‑149 (MQ=255) gCCGCGACTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GGGCGATGCTCg < 3:243510/149‑1 (MQ=255) gCCGCGACTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCg < 5:330439/149‑1 (MQ=255) cgACTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCct > 5:252145/1‑149 (MQ=255) cTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCta < 6:27965/149‑1 (MQ=255) gTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCtata > 7:91676/1‑149 (MQ=255) cGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACACCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCg > 8:292278/1‑149 (MQ=255) ggcgCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATc < 4:45040/149‑1 (MQ=255) ggcgCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATc < 3:86298/149‑1 (MQ=255) gcgcAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCg < 3:216897/149‑1 (MQ=255) ggTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTTCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCGTGCCTCTATACCCGCCAGCATCGCCAATTGAt < 1:33239/148‑1 (MQ=255) tGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCaa < 6:252145/149‑1 (MQ=255) tttCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTg > 8:148172/1‑148 (MQ=255) ttCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAAt < 8:174510/136‑1 (MQ=255) tCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATCCCCGCCAGCATCGCCAATTGAT‑GCAACTGGag > 3:172869/1‑149 (MQ=255) tCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTg > 1:315877/1‑146 (MQ=255) tCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGa < 2:58597/148‑1 (MQ=255) tCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGa < 7:148172/148‑1 (MQ=255) tCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGa < 2:315877/148‑1 (MQ=255) aTTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGag > 8:221817/1‑147 (MQ=255) cTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTACACCCGCCAGCATCGCCAATTGAT‑GCAACTGGAGCAGCGt > 6:201662/1‑149 (MQ=255) tCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGAGCAGCGTc > 8:130375/1‑149 (MQ=255) gCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGAGCAGCGTCTGCGCACGGc > 5:113285/1‑149 (MQ=255) cGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGAGCAGCGTCTGCGCACGGCCg < 2:321072/149‑1 (MQ=255) cGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTACACCCGCCAGCATCGCCAATTGAT‑GCAACTGGAGCAGCGTCTGCGCACGGCCg < 5:201662/149‑1 (MQ=255) ctcACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGAGCAGCGTCTGCGCACGGCCGGt < 5:103097/147‑1 (MQ=255) cACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGAGCAGCGTCTGCGCACGGCCGGTAt > 3:50533/1‑149 (MQ=255) ccGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GGGCGATGCTCgaa < 5:127952/82‑3 (MQ=255) ccGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GGGCGATGCTCgaa > 6:127952/1‑80 (MQ=255) ccGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACg > 5:231060/1‑149 (MQ=255) ccGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCAATGCGCCTGAAGGACTTCGAGCATC‑GCCCGAAGCTCG‑GCATCTATACACGCCAGCATCGCCAATTTAT‑GAAACAGGAGCAGCGTCTGCGCACGGCCGGTATCGACg > 4:68448/1‑149 (MQ=255) gtgtGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACgtg < 7:130375/149‑1 (MQ=255) tgctgcTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGAGCAGCGTCTgcgc > 8:206429/1‑128 (MQ=255) tgctgcTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGAGCAGCGTCTgcgc < 7:206429/128‑1 (MQ=255) tgGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCTGCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑g < 1:229034/102‑1 (MQ=255) tgGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑g > 2:229034/1‑101 (MQ=255) gCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAAGTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGa > 8:245571/1‑149 (MQ=255) gCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGa > 6:24896/1‑149 (MQ=255) gAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATcag > 3:156217/1‑147 (MQ=255) gAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCg < 6:231060/149‑1 (MQ=255) aaaCCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCgg < 7:292278/149‑1 (MQ=255) cGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGgccgc > 2:151686/1‑149 (MQ=255) atgCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGGCCGCCGGAGCGTTAcc < 6:204142/146‑1 (MQ=255) tGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGGCCGCCGGAGCGTTACCTCATGGAg > 1:65126/1‑149 (MQ=255) gcCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGATGCCGATATCCGGCCGCCGGAGCGTTACCTCATGGAgcgc < 5:319732/149‑1 (MQ=255) cTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGag > 5:98469/1‑72 (MQ=255) cTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGag < 6:98469/72‑1 (MQ=255) cTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGAGCAGCGGCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGGCCGCCGGAGCGTTACCTCATGGAGCGCtt < 4:172869/149‑1 (MQ=255) tGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGATAAGAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGGCCGCCGGAGCGTTACCTCATGGAGCGCtt > 1:253595/1‑149 (MQ=255) gAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGGCCGCCGGAGCGTTACCTCATGGAGCGCTtca > 4:224092/1‑149 (MQ=255) gACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGGCCGCCGGAGCGTTACCTCATGGAGCGCTTCATCAc < 1:66171/149‑1 (MQ=255) cGAGCATC‑GGGCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGGCCGCCGGAGCGTTACCTCATGGAGCGCTTCATCACGGCAc > 4:110194/1‑149 (MQ=255) aTC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAg > 2:35209/1‑92 (MQ=255) aTC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAg < 1:35209/92‑1 (MQ=255) aTC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGGCCGCCGGAGCGTTACCTCATGGAGCGCTTCATCACGGCACCGGTg > 6:183600/1‑149 (MQ=255) | CGATGCCGGGCCGCGACTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATC‑GCCCGATGCTCG‑GCCTCTATACCCGCCAGCATCGCCAATTGAT‑GCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGGCCGCCGGAGCGTTACCTCATGGAGCGCTTCATCACGGCACCGGTG > NC_002947/2730747‑2731038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |