Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,740,779 T→C 8.1% L1053P (CTG→CCG)  PP_2395 → leucine‑rich repeat‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,740,7790TC 8.1% 138.2 / 2.4 49L1053P (CTG→CCG) PP_2395leucine‑rich repeat‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (24/21);  new base C (2/2);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

CACGTCATGTTCTGCAATTTCAGATCGAGCGGCTTGCCCAACATACCCCCACAGGTCAGCTTCAGAGGTGTGACCAGCCTGTCGCTGCTGCACCTTGACCTTCTGGAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTTGCTGCAGATAGCGCAGCTGCGGCATCTGAGCCTGACCAACAACCGGATCGTCCTG‑AACATCGCACAAACCGCCACACTGGCCAGTTGCACGAGCCT  >  NC_002947/2740637‑2740926
                                                                                                                                              |                                                                                                                                                    
cACGTCATGTTCTGCAATTTCAGATCGAGCGGCTTGCCCAACATACCCCCACAGGTCAGCTTCAGAGGTGTGACCAGCCTGTCGCTGCTGCACCTTGACCTTCTGGAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAAc                                                                                                                                                >  3:132899/1‑149 (MQ=255)
   gTCATGTTCTGCAATTTCAGATCGAGCGGCTTGCCCAACATACCCCCACAGGTCAGCTTCAGAGGTGTGACCAGCCTGTCGCTGCTGCACCTTGACCTTCTGGAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAACCCt                                                                                                                                             <  3:212460/149‑1 (MQ=255)
   gTCATGTTCTGCAATTTCAGATCGAGCGGCTTGCCCAACATACCCCCACAGGTCAGCTTCAGAGGTGTGACCAGCCTGTCGCTGCTGCACCTAGACCTTCTGTAGGTGCCCAGCAGCATATTGCTGGCTTTCCCTAACCTGCAAAcac                                                                                                                                              >  6:256907/1‑146 (MQ=255)
        gTTCTGCAATTTCAGATCGAGCGGCTTGCCCAACATACCCCCACAGGTCAGCTTCAGAGGTGTGACCAGCCTGTCGCTGCTGCACCTTGACCTTCTGGAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCCGCAAACCCTCGATc                                                                                                                                        >  8:24891/1‑149 (MQ=255)
           cTGCAATTTCAGATCGAGCGGCTTGCCCAACATACCCCCACAGGTCAGCTTCAGAGGTGTGACCAGCCTGTCGCTGCTGCACCTTGACCTTCTGGAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTg                                                                                                                                      <  6:150156/148‑1 (MQ=255)
            tGCAATTTCAGATCGAGCGGCTTGCCCAACATACCCCCACAGGTCAGCTTCAGAGGTGTGACCAGCCTGTCGCTGCTGCACCTTGACCTTCTGGAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTgg                                                                                                                                     >  4:72686/1‑148 (MQ=255)
             gCAATTTCAGATCGAGCGGCTTGCCCAACATACCCCCACAGGTCAGCTTCAGAGGTGTGACCAGCCTGTCGCTGCTGCACCTTGACCTTCTGGAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAAc                                                                                                                                                <  6:26166/136‑1 (MQ=255)
             gCAATTTCAGATCGAGCGGCTTGCCCAACATACCCCCACAGGTCAGCTTCAGAGGTGTGACCAGCCTGTCGCTGCTGCACCTTGACCTTCTGGAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAAc                                                                                                                                                >  5:26166/1‑136 (MQ=255)
              cAATTTCAGATCGAGCGGCTTGCCCAACATACCCCCACAGGTCAGCTTCAGAGGTGTGACCAGCCTGTCGCTGCTGCACCTTGACCTTCTGGAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCCGCAAACCCTCGATCTgg                                                                                                                                     <  7:24891/146‑1 (MQ=255)
                     aGATCGAGCGGCTTGCCCAACAAAACCCCAAAGGTAAGCTTCAGAGGTGTGACAAGCATGTCGCTGCTGCACCTTGACCTTATGGAGGTGCCCAGCAGCCTATTGATGCATTTCCCTAACCTGAAAACCCACGATCTGGGAGGGAAcat                                                                                                                           >  7:202036/1‑147 (MQ=255)
                       aTCGAGCGGCTTGCCCAACATACCCCCACAGGTCAGCCTCAGAGGTGTGACCAGCCTGTCGCTGCTGCACCTTGACCTTCTGGAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGt                                                                                                                         <  8:43317/149‑1 (MQ=255)
                        tCGAGCGGCTTGCCCAACATACGCCCACAGGTCAGCTTCAGAGGTGTGACCAGCCTGTCGCTGCTGCACCTTGACCTTCTGGAGGTGCCGAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGtt                                                                                                                        <  5:101701/149‑1 (MQ=255)
                             cGGCTTGCCCAACATACCCCCACAGGTCAGCTTCAGAGGTGTGACCAGCCTGTCGCTGCTGCACCTTGACCTTCTGGAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGTTGAcgc                                                                                                                   <  8:175413/149‑1 (MQ=255)
                             cGGCTTGCCCAACATACCCCCACAGGTCAGCTTCAGAGGTGTGACCAGCCTGTCGCTGCTGCACCTTGACCTTCTGGAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGTTGAcgc                                                                                                                   <  3:72686/149‑1 (MQ=255)
                               gCTTGCCCAACATACCCCCACAGGTCAGCTTCAGAGGTGTGACCAGCCTGTCGCTGCTGCACCTTGACCTTCTGGAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGtt                                                                                                                        <  5:186411/142‑1 (MQ=255)
                               gCTTGCCCAACATACCCCCACAGGTCAGCTTCAGAGGTGTGACCAGCCTGTCGCTGCTGCACCTTGACCTTCTGGAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGtt                                                                                                                        >  6:186411/1‑142 (MQ=255)
                                             cccccACAGGTCAGCTTCAGAGGTGTGACCAGCCTGTCGCTGCTGCACCTTGACCTTCTGGAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGtt                                                                                                   <  2:86937/149‑1 (MQ=255)
                                                ccACAGGTCAGCTTCAGATGTGTGACCAGCCTGTCGCTGCTGCACCTTGACCTTCTTGAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTGGGAGGTAACCTGTTGACGCGCCTTCCGCAGCCGTtgct                                                                                                <  5:256907/149‑1 (MQ=255)
                                                ccACAGGTCAGCTTCAGAGGTGTGACCAGCCTGTCGCTGCTGCACCTTGACCTTCTGGAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTtgct                                                                                                >  3:221530/1‑149 (MQ=255)
                                                 cacaGGTCAGCTTCAGAGGTGTGACCAGCCTGTCGCTGCTGCACCTTGACCTTCTGGAGGTGCCCAGCAGCCCATTGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTtgctg                                                                                               >  1:47526/1‑149 (MQ=255)
                                                       tCAGCTTCAGAGGTGTGACCAGCCTGTCGCTGCTGCACCTTGACCTTCTGGAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTtgctg                                                                                               >  6:221460/1‑143 (MQ=255)
                                                       tCAGCTTCAGAGGTGTGACCAGCCTGTCGCTGCTGCACCTTGACCTTCTGGAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTtgctg                                                                                               <  5:221460/143‑1 (MQ=255)
                                                               agagGTGTGACCAGCCTGTCGCTGCTGCACCTTGACCTTCTGGAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTTGCTGCAGATAGCGCAGCt                                                                                 <  4:132899/149‑1 (MQ=255)
                                                                   gtgtGACCAGCCTGTCGCTGCTGCACCTTGACCTTCTGGAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTTGCTGCAGATAGCGCAGCTGCgg                                                                             >  1:49357/1‑149 (MQ=255)
                                                                       gACCAGCCTGTCGCTGCTGCACCTTGACCTTCTGGAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTTGCTGCAGATAGCGCAGCTGCGGCATc                                                                         >  5:7159/1‑149 (MQ=255)
                                                                          cAGCCTGTCGCTGCTGCACCTTGACCTTCTGGAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTTGCTGCAGATAGCGCAGCTGCGGCATCTGa                                                                      <  1:119636/149‑1 (MQ=255)
                                                                                 tCGCTGCTGCACCTTGACCTTCTGGAGGTGCCCAGCAGCCCATTGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTTGCTGCAGATAGCGCAGCTGCGGCATCTGAGCCTGa                                                                <  2:47526/148‑1 (MQ=255)
                                                                                         gcACCTTGACCTTCTGGAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCCGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTTGCTGCAGATAGCGCAGCTGCGGCATCTGAGCCTGACCAACAAcc                                                       >  2:201097/1‑149 (MQ=255)
                                                                                          cACCTTGACCTTCTGGAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTTGCTGCAGATAGCGCAGCTGCGGCATCTGAGCCTGACCAACAAcc                                                       <  2:49357/148‑1 (MQ=255)
                                                                                             cTTGACCTTCTGGAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTTGCTGCAGATAGCGCAGCTGCGGCATCTGAGCCTGACCAACAACCGGAt                                                   >  1:320812/1‑149 (MQ=255)
                                                                                             cTTGACCTTCTGGAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTTGCTGCAGATAGCGCAGCTGCGGCATCTGAGCCTGACAAAAAACCGGAt                                                   >  2:235173/1‑149 (MQ=255)
                                                                                                    ttCTGGAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTTGCTGCAGATAGCGCAGCTGCGGCATCTGAGCCTGACCAACAACCGGATCGTCCt                                             >  4:85962/1‑148 (MQ=255)
                                                                                                         gAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTTGCTGCAGATAGCGCAGCTGCGGCATCTGAGCCTGACCAACAACCGGATCGTCCTG‑aa                                         <  4:221530/146‑1 (MQ=255)
                                                                                                         gAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTTGCTGCAGATAGCGCAGCTGCGGCATCTGAGCCTGACCAACAACCGGATCGTCCTG‑AACAt                                      >  7:32538/1‑149 (MQ=255)
                                                                                                              gCCCAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTTGCTGCAGATAGCGCAGCTGCGGCATCTGAGCCTGACCAACAACCGGATCGTCCTG‑AACATCg                                    >  7:121566/1‑146 (MQ=255)
                                                                                                              gCCCAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTTGCTGCAGATAGCGCAGCTGCGGCATCTGAGCCTGACCAACAACCGGATCGTCCTG‑AACATCg                                    <  8:121566/146‑1 (MQ=255)
                                                                                                                     agcCTATTGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTTGCTGCAGATAGCGCAGCTGCGGCATCTGAGCCTGACCAACAACCGGATCGTCCTG‑AACATCGCACAAACCGc                          <  2:320812/149‑1 (MQ=255)
                                                                                                                            tGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTTGCTGCAGATAGCGCAGCTGCGGCATCTGAGCCTGACCAACAACCGGATCGTCCTg                                            >  5:268656/1‑125 (MQ=255)
                                                                                                                            tGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTTGCTGCAGATAGCGCAGCTGCGGCATCTGAGCCTGACCAACAACCGGATCGTCCTg                                            <  6:268656/125‑1 (MQ=255)
                                                                                                                                   tttCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTTGCTGCAGATAGCGCAGCTGCGGCATCTGAGCCTGACCAACAACCGGATCGTCCTG‑AACATc                                     <  4:263157/124‑1 (MQ=255)
                                                                                                                                   tttCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTTGCTGCAGATAGCGCAGCTGCGGCATCTGAGCCTGACCAACAACCGGATCGTCCTG‑AACATc                                     >  3:263157/1‑124 (MQ=255)
                                                                                                                                   tttCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTTGCTGCAGATAGCGCAGCTGCGGCATCTGAGCCTGACCAACAACCGGATCGTCCTG‑AACATCGCACAAACCGCCACACTGGCCAGtt            >  3:194227/1‑149 (MQ=255)
                                                                                                                                       ccTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTTGCTGCAGATAGCGCAGCTGCGGCATCTGAGCCTGACCAACAACCGGATCGTCCTG‑AACATCGCACAAACCGCCAGACTCGCCAGTTGc          >  8:276687/1‑147 (MQ=255)
                                                                                                                                       ccTAACCCGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTTGCTGCAGATAGCGCAGCTGCGGCATCTGAGCCTGACCAACAACCGGATCGTCCTGAAACATCGCACAAACCGCCACACTGGCCAGTTGCa         <  1:201097/149‑1 (MQ=255)
                                                                                                                                        cTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTTGCTGCAGATAGCGCAGCTGCGGCATCTGAGCCTGACCAACAACCGGATCGTCCTG‑AACATCGCACAAACCGCCACACTGGCCAGTTGCAc        >  7:110465/1‑148 (MQ=255)
                                                                                                                                            ccTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTTGCTGCAGATAGCGCAGCTGCGGCATCTGAGCCTGACCAACAACCGGATCGTCCTG‑AACAt                                      <  7:275725/114‑1 (MQ=255)
                                                                                                                                            ccTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTTGCTGCAGATAGCGCAGCTGCGGCATCTGAGCCTGACCAACAACCGGATCGTCCTG‑AACAt                                      >  8:275725/1‑114 (MQ=255)
                                                                                                                                            ccTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTTGCTGCAGATAGCGCAGCTGCGGCATCTGAGCCTGACCAACAACCGGATCGTCCTG‑AACATCGCACAAACCGCCACACTGGCCAGTTGCACGAGcc   >  4:140147/1‑149 (MQ=255)
                                                                                                                                             cTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTTGCTGCAGATAGCGCAGCTGCGGCATCTGAGCCTGACCAACAACCGGATCGTCCTG‑AACATCGCACAAACCGCCACACTGGCCAGTTGCACGAGCCt  >  6:220510/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
CACGTCATGTTCTGCAATTTCAGATCGAGCGGCTTGCCCAACATACCCCCACAGGTCAGCTTCAGAGGTGTGACCAGCCTGTCGCTGCTGCACCTTGACCTTCTGGAGGTGCCCAGCAGCCTATTGCTGGCTTTCCCTAACCTGCAAACCCTCGATCTGGGAGGGAACCTGTTGACGCGCCTTCCGCAGCCGTTGCTGCAGATAGCGCAGCTGCGGCATCTGAGCCTGACCAACAACCGGATCGTCCTG‑AACATCGCACAAACCGCCACACTGGCCAGTTGCACGAGCCT  >  NC_002947/2740637‑2740926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑