Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,744,544	T→G	8.2%	V28G (GTC→GGC)	PP_2399 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,744,544	0	T	G	8.2%	168.4 / 7.4	62	V28G (GTC→GGC)	PP_2399	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (25/30);  new base G (3/2);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 6.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.46e-01

CGAACTGTTGGCCGAGACGGCGCGTATGCGTGCCGTGCAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGAGCAGCG‑CTGGCATGCCCGAGTACGTGATTTCTACGCCAGTTACCG  >  NC_002947/2744400‑2744688                                                                                                                                                  |                                                                                                                                                  cGAACTGTTGGCCGAGACGGCGCGTATGCGTGCCGTGCAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAg                                                                                                                                               >  3:263936/1‑149 (MQ=255) cGAACTGTTGGCCGAGACGGCGCGTATGCGTGCCGTGCAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAg                                                                                                                                               <  4:143331/149‑1 (MQ=255)        ttGGCCGAGACGGCGCGTATGCGTGCCGTGCAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGgc                                                                                                                                        >  5:49404/1‑149 (MQ=255)            ccGAGACGGGGCGTATGCGTGGCGTGCAATGCGCTTGCGGTGGGCAATCAGTTGAATGAGACCCTGCGTGCGCAGGAGTATGCCTTGGCCCGTCCCCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGCCGGGCCTCGACGccg                                                                                                                                    <  8:50281/149‑2 (MQ=255)               agaCGGCGCGTATGCGTGCCGTGCAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGaa                                                                                                                                 <  6:49404/149‑1 (MQ=255)                gaCGGCGCGTATGCGTGCCGTGCAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAg                                                                                                                                <  5:230956/149‑1 (MQ=255)                gaCGGCGCGTATGCGTGCCGTGCAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAg                                                                                                                                <  8:107648/149‑1 (MQ=255)                gaCGGCGCGTATGCGTGCCGTGCAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAg                                                                                                                                <  7:265290/149‑1 (MQ=255)                              gtgccgtgcAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCg                                                                                                                                                 <  8:117967/118‑1 (MQ=255)                              gtgacgtgcAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCg                                                                                                                                                 >  7:117967/1‑118 (MQ=255)                                 ccgtgcAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAgg                                                                                                                               <  1:285791/133‑1 (MQ=255)                                 ccgtgcAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAgttgtt                                                                                                               <  3:291303/149‑1 (MQ=255)                                 cagtgcAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTACGCGACACACACCGCC‑ATCCCCCGCCGCCAGGCGTCGAGGCCCCGCCCCTGaacg                                                                                                                               >  2:285791/3‑131 (MQ=255)                                     gcAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTAt                                                                                                           >  2:240144/1‑149 (MQ=255)                                      cAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATg                                                                                                          <  2:240358/149‑1 (MQ=255)                                        aTGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGa                                                                                                                     >  4:52721/1‑136 (MQ=255)                                        aTGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGa                                                                                                                     <  3:52721/136‑1 (MQ=255)                                           cgcTGCCGGAGGGCAAGCAGATGAATGACACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGgcgc                                                                                                      <  4:241783/148‑1 (MQ=255)                                                ccGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCg                                                                                                 >  3:230262/1‑148 (MQ=255)                                                   gAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAgg                                                                                             <  1:240144/149‑1 (MQ=255)                                                    aGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGc                                                                                            >  5:141532/1‑149 (MQ=255)                                                      ggCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCtt                                                                                          >  5:71005/1‑149 (MQ=255)                                                         aaGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTgct                                                                                       <  6:257935/149‑1 (MQ=255)                                                             aGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTgctggct                                                                                   <  4:230262/149‑1 (MQ=255)                                                                 gaatgaGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAgttgtt                                                                                                               >  1:309041/1‑117 (MQ=255)                                                                 gaatgaGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAgttgtt                                                                                                               <  2:309041/117‑1 (MQ=255)                                                                    tgaGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCt                                                                            <  4:111335/149‑1 (MQ=255)                                                                       gaCCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTgctgg                                                                                     <  4:296576/137‑1 (MQ=255)                                                                        aCCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTccc                                                                        >  6:133797/1‑149 (MQ=255)                                                                          ccTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCgg                                                                      >  5:41587/1‑149 (MQ=255)                                                                            tgcgttcgCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGACGAGGCCCCGCGCCAGAAGGGTTACCGTGAGGAGTTCTATGCCGCCATCGAAGGCTTGCAGGCTGGCAGATTCCGGGt                                                                     >  8:289930/1‑148 (MQ=255)                                                                             gcgtgcgCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGa                                                                   >  1:290709/1‑149 (MQ=255)                                                                               gtgcgCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCACCGCCGCACGGAGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCAGGCTGGCAGCTGCCCGGTGATg                                                                 >  1:292694/1‑149 (MQ=255)                                                                                    cagcagTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCa                                                            <  4:109189/149‑1 (MQ=255)                                                                                            tGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTGTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTgg                                                    <  5:160299/149‑1 (MQ=255)                                                                                              ccTTGGCCCGTCACCTGCGCGACCCACAGCGCCAATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTggg                                                   >  1:205352/1‑149 (MQ=255)                                                                                              ccTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATc                                                                                                  <  4:130522/101‑1 (MQ=255)                                                                                              ccTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTTATGCTCCAGGCGTTTGGc                                                  >  4:72611/1‑149 (MQ=255)                                                                                                     ccGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGa                                                <  8:54121/144‑1 (MQ=255)                                                                                                     ccGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGa                                                >  7:54121/1‑144 (MQ=255)                                                                                                        tCACGTTCGCGACCCTCAGCGAG‑ATGCCCCGCCGCCCGGCGTCGAGGGCCTGCGCCTGAATGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTTCTGGCTGGCAGCTTCCCGGTTATTCGCCAGGCGTTGGGCGAGCAGCG‑Ct                                       <  2:292694/149‑1 (MQ=255)                                                                                                          aCCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATg                                                                 <  6:203770/122‑1 (MQ=255)                                                                                                          aCCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATg                                                                 >  5:203770/1‑122 (MQ=255)                                                                                                            cTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCACCTTCCCGGTGATGCGCCAGGCGTTGGGCGAGCAGCG‑CTGGCa                                   >  1:113417/1‑149 (MQ=255)                                                                                                              gcgcAACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGAGCAGCG‑CTGGc                                    >  1:199920/1‑146 (MQ=255)                                                                                                              gcgcAACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGAGCAGCG‑CTGGc                                    <  2:199920/146‑1 (MQ=255)                                                                                                                 cgACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATgc                                                                <  3:2335/116‑1 (MQ=255)                                                                                                                 cgACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATgc                                                                >  4:2335/1‑116 (MQ=255)                                                                                                                      cacaGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGAGCAGCG‑CTGGCATGCCCGAGTa                         <  6:41587/149‑1 (MQ=255)                                                                                                                      cacaGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCA‑GCCTTTGGCCAGCAACGCCTGGCTGGCCCGAgaa                         >  2:221336/1‑147 (MQ=255)                                                                                                                       acaGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGAGCAGCG‑CTGGCATGCCCGAGTAc                        >  5:57072/1‑149 (MQ=255)                                                                                                                          gcgcC‑GGGCCTCGACGCCGGGCGGCGGGGCATGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCAGTGATGCGCCAGGCGTTg                                                     <  7:218689/118‑1 (MQ=25)                                                                                                                          gcgcC‑GGGCCTCGACGCCGGGCGGCGGGGCATGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCAGTGATGCGCCAGGCGTTg                                                     >  8:218689/1‑118 (MQ=25)                                                                                                                             gccgggCCTCGACGCCGGGCGGCGGGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGag                                               <  6:265837/117‑1 (MQ=255)                                                                                                                             gccgggCCTCGACGCCGGGCGGCGGGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGag                                               >  5:265837/6‑122 (MQ=255)                                                                                                                             gccgggCCTCGACGCCGGGCGGCGGGGCATGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGAGCAGCG‑CTGGCATGCCCGAGTACGTGAt                   >  8:164799/6‑149 (MQ=38)                                                                                                                              c‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGAGCAGCG‑CTGGCATGCCCGa                            <  4:178477/138‑1 (MQ=255)                                                                                                                              c‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGAGCAGCG‑CTGGCATGCCCGa                            >  3:178477/1‑138 (MQ=255)                                                                                                                              c‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGAGCAGCG‑CTGGCATGCCCGAGTACGTGAtt                  <  7:36854/148‑1 (MQ=255)                                                                                                                              c‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGAGCAGCG‑CTGGCATGCCCGAGTACGTGAtt                  >  8:36854/1‑148 (MQ=255)                                                                                                                                     ccgccgCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGAGCAGCG‑CTGGc                                    <  6:173390/124‑1 (MQ=255)                                                                                                                                              ggCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGAGCAGCG‑CTGGCATGCCCGAGTACGTGATTTCTACGCCAGTTACCg  <  6:57072/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                  CGAACTGTTGGCCGAGACGGCGCGTATGCGTGCCGTGCAATGCGCTGCCGGAGGGCAAGCAGATGAATGAGACCCTGCGTGCGCAGCAGTATGCCTTGGCCCGTCACCTGCGCGACCCACAGCGCC‑ATGCCCCGCCGCCCGGCGTCGAGGCCCGGCGCCTGAAGGTTTACCGTGAGTTGTTCTATGGCGCCATCGAAGGCTTGCTGGCTGGCAGCTTCCCGGTGATGCGCCAGGCGTTGGGCGAGCAGCG‑CTGGCATGCCCGAGTACGTGATTTCTACGCCAGTTACCG  >  NC_002947/2744400‑2744688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑