Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,755,086 G→C 8.3% G471A (GGG→GCG) ‡ czcA‑II → cation efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,755,0860GC 8.3% 152.5 / 4.1 48G471A (GGG→GCG) ‡czcA‑IIcation efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (21/23);  new base C (2/2);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

CCCATGCCCAGCAGCGCCACGGTCGCATGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGTGATGC  >  NC_002947/2754939‑2755232
                                                                                                                                                    |                                                                                                                                                   
cccATGCCCAGCAGCGCCACGGTCGCATGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCggg                                                                                                                                                    >  1:79607/1‑149 (MQ=255)
 ccATGCCCAGCAGCGCCACGGTCGCATGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCgggg                                                                                                                                                   >  4:207849/1‑149 (MQ=255)
     gCCCAGCAGCGCCACGGTCGCATGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTc                                                                                                                                                 >  3:128730/1‑147 (MQ=255)
            agcGCCACGGTCGCATGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTCGACCCCGGTC‑AGGGC‑aaa                                                                                                                                       <  5:266920/149‑2 (MQ=25)
               gcCACGGTCGCATGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTCGACCCCGGTC‑AGGGC‑aaagat                                                                                                                                    <  5:50481/149‑5 (MQ=25)
               gcCACGGTCGCATGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTCGACCCCGGTC‑AGGGC‑a                                                                                                                                         >  6:50481/1‑144 (MQ=25)
                    ggTCGCATGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTTGAGGGC‑AAGATGTTCCa                                                                                                                               <  8:156250/149‑1 (MQ=255)
                    ggTCGCATGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCa                                                                                                                               >  2:34723/1‑149 (MQ=255)
                     gTCGCATGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCAc                                                                                                                              <  2:269938/149‑1 (MQ=255)
                        gCATGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCAccc                                                                                                                            >  4:83028/1‑148 (MQ=255)
                         cATGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTa                                                                                                                          >  3:183738/1‑149 (MQ=255)
                              tGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGcc                                                                                                                     >  5:12000/1‑149 (MQ=255)
                                aCCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCtt                                                                                                                   >  4:55599/1‑149 (MQ=255)
                                  ccGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTa                                                                                                                 >  8:182117/1‑149 (MQ=255)
                                     cggcgAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCg                                                                                                              <  3:55599/149‑1 (MQ=255)
                                         gAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAggg                                                                                                                                            <  5:49877/116‑1 (MQ=255)
                                         gAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAggg                                                                                                                                            >  6:49877/1‑116 (MQ=255)
                                          aaCGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTc                                                                                                         <  3:207849/149‑1 (MQ=255)
                                            cGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCAt                                                                                                       >  4:92981/1‑149 (MQ=255)
                                              gTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATgg                                                                                                     <  7:182117/149‑1 (MQ=255)
                                               ttCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATg                                                                                                                                    <  7:50144/117‑1 (MQ=255)
                                               ttCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATg                                                                                                                                    >  8:50144/1‑117 (MQ=255)
                                               ttCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGc                                                                                                    >  8:76899/1‑149 (MQ=255)
                                                   aTGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTg                                                                                                <  5:261288/149‑1 (MQ=255)
                                                      aGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTg                                                                                             <  2:30887/149‑1 (MQ=255)
                                                           tttGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGgcgc                                                                                        >  3:301847/1‑149 (MQ=255)
                                                           tttGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGgcgc                                                                                        <  7:76899/149‑1 (MQ=255)
                                                               ccGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCgatg                                                                                    <  5:168379/149‑1 (MQ=255)
                                                                cGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCgatga                                                                                   <  3:83028/149‑1 (MQ=255)
                                                                  cTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATc                                                                                 <  4:183738/149‑1 (MQ=255)
                                                                  cTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATc                                                                                 <  3:92981/149‑1 (MQ=255)
                                                                        gcgAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCg                                                                           <  2:79607/149‑1 (MQ=255)
                                                                         cgAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCgg                                                                          >  2:40187/1‑149 (MQ=255)
                                                                         cgAAGCACGCCGACCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCgg                                                                          >  1:175547/1‑149 (MQ=255)
                                                                                                 agggCAGTTGATCATCATGGTCGACTACCAGCCGAACTTTGACCT‑GACCGGGGTCAAGGCC‑AAGATGTTCCACACTATCGCCTTTACCGTGGTCATGGCCCAGTTGGGCTCTAAGATCCTTACGGTCACTTTCATACCGGCAGCCAtt                                                   >  8:122201/2‑148 (MQ=255)
                                                                                                      aGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTg                                             >  1:190769/1‑149 (MQ=255)
                                                                                                           atcatcATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACgttcgttc                                                              <  8:178626/127‑1 (MQ=255)
                                                                                                           atcatcATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACgttcgttc                                                              >  7:178626/1‑127 (MQ=255)
                                                                                                              atcatGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCAc                                     <  2:190769/149‑1 (MQ=255)
                                                                                                               tcatGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCAcc                                    >  1:180873/1‑149 (MQ=255)
                                                                                                                    gtggttTACCTGCCTATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCaa                               <  7:122201/149‑1 (MQ=255)
                                                                                                                      ggtgTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAgg                             <  2:180873/149‑1 (MQ=255)
                                                                                                                          tACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCaa                         <  1:34723/149‑1 (MQ=255)
                                                                                                                             cTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAagg                       >  6:260126/1‑148 (MQ=255)
                                                                                                                                 cGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGcc                                                >  2:272133/1‑119 (MQ=255)
                                                                                                                                    atcttGCCCTCGACCCCGGTC‑AGGGCAAAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAaggaagagg                 >  4:260392/4‑148 (MQ=255)
                                                                                                                                     cTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGccc                                               >  6:297675/1‑116 (MQ=255)
                                                                                                                                     cTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGccc                                               <  5:297675/116‑1 (MQ=255)
                                                                                                                                        tGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAgg             <  6:12000/147‑1 (MQ=255)
                                                                                                                                                  cGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGTGATGc  <  4:303651/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCCATGCCCAGCAGCGCCACGGTCGCATGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGC‑AAGATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGTGATGC  >  NC_002947/2754939‑2755232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑