Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,793,756 C→A 12.8% intergenic (+65/+62) PP_2445 → / ← PP_2446 membrane protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,793,7560CA12.8% 126.0 / 10.3 47intergenic (+65/+62)PP_2445/PP_2446membrane protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (18/23);  new base A (3/3);  total (21/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.33e-01

ATGGCGTTCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGGTGTGCATGAC  >  NC_002947/2793611‑2793900
                                                                                                                                                 |                                                                                                                                                
aTGGCGTTCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTgg                                                                                                                                               <  8:233890/149‑1 (MQ=255)
  ggCGTTCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGc                                                                                                                                              >  5:52830/1‑148 (MQ=255)
   gCGTTCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCaa                                                                                                                                            >  7:15461/1‑149 (MQ=255)
   gCGTTCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCaa                                                                                                                                            <  1:194170/149‑1 (MQ=255)
      ttCTCGACGTCTATCGAGGTCCTCAACATCCTGGCCCGGCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAACGGGGCGACTCGCGCCCCTCTTGCTTACAAGCCCGCTGCACAGGTATTCGCGTGGCACTTGCAAcgc                                                                                                                                         <  4:277320/149‑1 (MQ=255)
          cGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCATTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGaa                                                                                                                                      >  5:262315/1‑148 (MQ=255)
          agACGGGTATCGATGTCCTCAACATCCTTGCCAGTCGAGCGCGTTTGAAGCGTGTGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGaa                                                                                                                                      <  7:140570/147‑1 (MQ=255)
               gCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCtg                                                                                                                                <  6:52830/149‑1 (MQ=255)
                cTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCtgt                                                                                                                               <  3:26610/149‑1 (MQ=255)
                     gAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAg                                                                                                                           <  2:65910/148‑1 (MQ=255)
                        gTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCggg                                                                                                                       <  7:296867/149‑1 (MQ=255)
                             cAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCACCGCGAATACCTGTGCAGCGGGCTTgt                                                                                                                  >  6:98288/1‑149 (MQ=255)
                             cAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTgt                                                                                                                  >  5:135116/1‑149 (MQ=255)
                                       gCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCCCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTgg                                                                                                        >  2:30433/1‑149 (MQ=255)
                                            tCGAGCGCGTTTGACGCGTGAGGCGGCTGAAGGTTGATGTAAAACGGGGCCCCTCGCGCCCCTCTTGCGGACACGCCCGCTGCCCAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTc                                                                                                   <  3:11327/149‑1 (MQ=39)
                                              gAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCgg                                                                                                 <  8:15461/149‑1 (MQ=255)
                                               aGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGGGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGGTTGTCAGTCAGTGGGCTTCgg                                                                                                 >  1:143081/1‑148 (MQ=255)
                                               aGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGTGCCACGCGAAt                                                                                                                                     >  3:236795/1‑112 (MQ=25)
                                               aGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGTGCCACGCGAAt                                                                                                                                     <  4:236795/112‑1 (MQ=25)
                                                         acgCGTGAGGCGGCTTAAGGTTTATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAg                                                                                      <  7:283935/147‑1 (MQ=255)
                                                                    ggcTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCCGTGGGCTTCGGGAAATCCTGAGGt                                                                                    <  1:291810/140‑1 (MQ=255)
                                                                      cTGAAGGTTGATGTAAAAAGGGGCGCCCCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCAcccc                                                                         <  1:30433/149‑1 (MQ=255)
                                                                        gAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGTGCCACGCGAATACCtgt                                                                                                                               >  7:121036/1‑93 (MQ=18)
                                                                        gAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGTGCCACGCGAATACCtgt                                                                                                                               <  8:121036/93‑1 (MQ=18)
                                                                        gAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGTGCCACGCGAATACCt                                                                                                                                 <  6:70629/91‑1 (MQ=18)
                                                                        gAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGTGCCACGCGAATACCt                                                                                                                                 >  5:70629/1‑91 (MQ=18)
                                                                         aaGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCgg                                                                       >  2:286911/1‑148 (MQ=255)
                                                                            gTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATg                                                                   >  3:261223/1‑149 (MQ=255)
                                                                             ttGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATgc                                                                  >  2:55238/1‑149 (MQ=255)
                                                                                  cgcaagaGGGGCGCGAGGCGCCACTCTTGCGGACAAGCCCGCAGCACACGTATTAGAGGGGCACAGGCAACGCGAATACCCGTGCAGCGGGCATGTAAGTCAGTGGGCTTCGGGAAATCCTGAAGTTAGCTAAACCCAGCATgccgccc                                                             >  6:211067/7‑148 (MQ=255)
                                                                                         ggggCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGt                                                      <  5:98288/149‑1 (MQ=255)
                                                                                          gggCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTc                                                     <  6:135116/149‑1 (MQ=255)
                                                                                               ccTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACt                                                >  1:174518/1‑149 (MQ=255)
                                                                                                   ccgcCCCTCTTGCGCACAAGCCCCCTGCACAGGTATTCGCGGGCCACTGGCACCGCTAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTgg                                              <  1:149822/146‑1 (MQ=38)
                                                                                                               cGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCgg                                <  4:261223/149‑1 (MQ=255)
                                                                                                                    aaGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGcc                           >  2:169618/1‑149 (MQ=255)
                                                                                                                     aGCCCGCTGCACCGGTATTCGCGTGGCACTGGCAACGCTAATACGTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCt                          <  5:211067/149‑1 (MQ=255)
                                                                                                                       cccGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGc                        >  3:275320/1‑149 (MQ=255)
                                                                                                                           cTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATc                                                                                           <  2:114873/78‑1 (MQ=255)
                                                                                                                           cTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATc                                                                                           >  1:114873/1‑78 (MQ=255)
                                                                                                                               acaGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAg                <  6:262315/149‑1 (MQ=255)
                                                                                                                               acaGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAg                >  5:11294/1‑149 (MQ=255)
                                                                                                                                caGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAgg               <  1:286911/149‑1 (MQ=255)
                                                                                                                                 aGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAggt              >  5:226488/1‑149 (MQ=255)
                                                                                                                                     aTTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGgtggtg          >  4:177668/1‑149 (MQ=255)
                                                                                                                                         gcgTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGGTGTGc       <  7:179031/148‑1 (MQ=255)
                                                                                                                                             ggCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGGTGTGCATGAc  <  2:174518/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ATGGCGTTCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGGTGTGCATGAC  >  NC_002947/2793611‑2793900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑