Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,793,761 A→C 13.4% intergenic (+70/+57) PP_2445 → / ← PP_2446 membrane protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,793,7610AC13.4% 109.6 / 11.3 45intergenic (+70/+57)PP_2445/PP_2446membrane protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (17/22);  new base C (3/3);  total (20/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.71e-01

GCGTTCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGGTGTGCATGAC  >  NC_002947/2793614‑2793900
                                                                                                                                                   |                                                                                                                                           
gCGTTCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCaa                                                                                                                                            >  7:15461/1‑149 (MQ=255)
gCGTTCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCaa                                                                                                                                            <  1:194170/149‑1 (MQ=255)
   ttCTCGACGTCTATCGAGGTCCTCAACATCCTGGCCCGGCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAACGGGGCGACTCGCGCCCCTCTTGCTTACAAGCCCGCTGCACAGGTATTCGCGTGGCACTTGCAAcgc                                                                                                                                         <  4:277320/149‑1 (MQ=255)
       cGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCATTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGaa                                                                                                                                      >  5:262315/1‑148 (MQ=255)
       agACGGGTATCGATGTCCTCAACATCCTTGCCAGTCGAGCGCGTTTGAAGCGTGTGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGaa                                                                                                                                      <  7:140570/147‑1 (MQ=255)
            gCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCtg                                                                                                                                <  6:52830/149‑1 (MQ=255)
             cTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCtgt                                                                                                                               <  3:26610/149‑1 (MQ=255)
                  gAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAg                                                                                                                           <  2:65910/148‑1 (MQ=255)
                     gTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCggg                                                                                                                       <  7:296867/149‑1 (MQ=255)
                          cAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCACCGCGAATACCTGTGCAGCGGGCTTgt                                                                                                                  >  6:98288/1‑149 (MQ=255)
                          cAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTgt                                                                                                                  >  5:135116/1‑149 (MQ=255)
                                    gCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCCCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTgg                                                                                                        >  2:30433/1‑149 (MQ=255)
                                         tCGAGCGCGTTTGACGCGTGAGGCGGCTGAAGGTTGATGTAAAACGGGGCCCCTCGCGCCCCTCTTGCGGACACGCCCGCTGCCCAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTc                                                                                                   <  3:11327/149‑1 (MQ=39)
                                           gAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCgg                                                                                                 <  8:15461/149‑1 (MQ=255)
                                            aGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGGGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGGTTGTCAGTCAGTGGGCTTCgg                                                                                                 >  1:143081/1‑148 (MQ=255)
                                            aGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGTGCCACGCGAAt                                                                                                                                     >  3:236795/1‑112 (MQ=25)
                                            aGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGTGCCACGCGAAt                                                                                                                                     <  4:236795/112‑1 (MQ=25)
                                                      acgCGTGAGGCGGCTTAAGGTTTATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAg                                                                                      <  7:283935/147‑1 (MQ=255)
                                                                 ggcTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCCGTGGGCTTCGGGAAATCCTGAGGt                                                                                    <  1:291810/140‑1 (MQ=255)
                                                                   cTGAAGGTTGATGTAAAAAGGGGCGCCCCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCAcccc                                                                         <  1:30433/149‑1 (MQ=255)
                                                                     gAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGTGCCACGCGAATACCtgt                                                                                                                               <  8:121036/93‑1 (MQ=18)
                                                                     gAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGTGCCACGCGAATACCtgt                                                                                                                               >  7:121036/1‑93 (MQ=18)
                                                                     gAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGTGCCACGCGAATACCt                                                                                                                                 <  6:70629/91‑1 (MQ=18)
                                                                     gAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGTGCCACGCGAATACCt                                                                                                                                 >  5:70629/1‑91 (MQ=18)
                                                                      aaGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCgg                                                                       >  2:286911/1‑148 (MQ=255)
                                                                         gTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATg                                                                   >  3:261223/1‑149 (MQ=255)
                                                                          ttGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATgc                                                                  >  2:55238/1‑149 (MQ=255)
                                                                               cgcaagaGGGGCGCGAGGCGCCACTCTTGCGGACAAGCCCGCAGCACACGTATTAGAGGGGCACAGGCAACGCGAATACCCGTGCAGCGGGCATGTAAGTCAGTGGGCTTCGGGAAATCCTGAAGTTAGCTAAACCCAGCATgccgccc                                                             >  6:211067/7‑148 (MQ=255)
                                                                                      ggggCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGt                                                      <  5:98288/149‑1 (MQ=255)
                                                                                       gggCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTc                                                     <  6:135116/149‑1 (MQ=255)
                                                                                            ccTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACt                                                >  1:174518/1‑149 (MQ=255)
                                                                                                ccgcCCCTCTTGCGCACAAGCCCCCTGCACAGGTATTCGCGGGCCACTGGCACCGCTAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTgg                                              <  1:149822/146‑1 (MQ=38)
                                                                                                            cGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCgg                                <  4:261223/149‑1 (MQ=255)
                                                                                                                 aaGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGcc                           >  2:169618/1‑149 (MQ=255)
                                                                                                                  aGCCCGCTGCACCGGTATTCGCGTGGCACTGGCAACGCTAATACGTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCt                          <  5:211067/149‑1 (MQ=255)
                                                                                                                    cccGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGc                        >  3:275320/1‑149 (MQ=255)
                                                                                                                        cTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATc                                                                                           <  2:114873/78‑1 (MQ=255)
                                                                                                                        cTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATc                                                                                           >  1:114873/1‑78 (MQ=255)
                                                                                                                            acaGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAg                <  6:262315/149‑1 (MQ=255)
                                                                                                                            acaGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAg                >  5:11294/1‑149 (MQ=255)
                                                                                                                             caGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAgg               <  1:286911/149‑1 (MQ=255)
                                                                                                                              aGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAggt              >  5:226488/1‑149 (MQ=255)
                                                                                                                                  aTTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGgtggtg          >  4:177668/1‑149 (MQ=255)
                                                                                                                                      gcgTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGGTGTGc       <  7:179031/148‑1 (MQ=255)
                                                                                                                                          ggCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGGTGTGCATGAc  <  2:174518/149‑1 (MQ=255)
                                                                                                                                                 gCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTgg                   >  7:294461/1‑125 (MQ=255)
                                                                                                                                                 gCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTgg                   <  8:294461/125‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                           
GCGTTCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGGTGTGCATGAC  >  NC_002947/2793614‑2793900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑