Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,826,825 G→C 12.8% A80P (GCA→CCA)  PP_2479 → cytochrome c family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,826,8250GC12.8% 126.7 / 10.6 47A80P (GCA→CCA) PP_2479cytochrome c family protein
Reads supporting (aligned to +/- strand):  ref base G (18/23);  new base C (4/2);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 3.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01

GCGAATACCTGACGCGCGCTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGC  >  NC_002947/2826679‑2826968
                                                                                                                                                  |                                                                                                                                                
gCGAATACCTGACGCGCGCTGCCAACTGTGTGGCTTGCCATTCCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCa                                                                                                                                                <  5:281151/149‑1 (MQ=255)
     tACCTGACGCGCGCTGCCAACTGTGGCGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTCCATGCAc                                                                                                                                           <  8:293865/149‑1 (MQ=255)
        cTGACGCGCGCTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGa                                                                                                                                        >  1:277179/1‑149 (MQ=255)
          gACGCGCGCTGCCAGCTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGAt                                                                                                                                       >  7:273639/1‑148 (MQ=255)
          gACGCGCGCTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGAt                                                                                                                                       >  7:243305/1‑148 (MQ=255)
               gcgcTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAg                                                                                                                                 >  2:259260/1‑149 (MQ=255)
                cgcTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGt                                                                                                                                <  3:266828/149‑1 (MQ=255)
                  cTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTc                                                                                                                              <  1:259260/149‑1 (MQ=255)
                  cTGCCAACTGTGTGGCGTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTc                                                                                                                              <  4:71524/149‑1 (MQ=255)
                   tGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCg                                                                                                                             >  4:262993/1‑149 (MQ=255)
                     ccAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGt                                                                                                                            >  5:165979/1‑148 (MQ=255)
                      cAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGCCGAGTTCGTCa                                                                                                                          <  8:235756/149‑1 (MQ=255)
                      cAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCa                                                                                                                          <  3:90946/149‑1 (MQ=255)
                                     ccATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCg                                                                                                           >  6:265827/1‑149 (MQ=255)
                                      cATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTgcgc                                                                                                                    <  6:296401/139‑1 (MQ=255)
                                      cATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTgcgc                                                                                                                    >  5:296401/1‑139 (MQ=255)
                                          ccGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGt                                                                                                                            >  1:273369/1‑127 (MQ=255)
                                          ccGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGt                                                                                                                            <  2:273369/127‑1 (MQ=255)
                                                         aaCCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAg                                                                                      <  2:277179/149‑1 (MQ=255)
                                                         aaCCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTCCATGCACCGATgc                                                                                                                                     <  6:245667/103‑2 (MQ=255)
                                                         aaCCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTCCATGCACCGATgc                                                                                                                                     >  5:245667/1‑102 (MQ=255)
                                                            ccTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGTCCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCac                                                                                   <  8:243305/149‑1 (MQ=255)
                                                                cGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCacta                                                                                 <  2:323968/147‑1 (MQ=255)
                                                                cGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCacta                                                                                 <  8:273639/147‑1 (MQ=255)
                                                                     gtggtgTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCg                                                                          <  6:165979/149‑1 (MQ=255)
                                                                           ttGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGttt                                                                    >  1:182322/1‑149 (MQ=255)
                                                                           ttGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGttt                                                                    >  2:268609/1‑149 (MQ=255)
                                                                              aGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTccc                                                                 <  5:265827/149‑1 (MQ=255)
                                                                              aGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTcc                                                                  <  3:262993/148‑1 (MQ=255)
                                                                                         ccGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTa                                                      <  6:55529/149‑1 (MQ=255)
                                                                                          cGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTAc                                                     <  3:190094/149‑1 (MQ=255)
                                                                                                gCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCaaa                                               <  3:222775/149‑1 (MQ=255)
                                                                                                           tCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATATCGCGCGa                                    >  1:110579/1‑149 (MQ=255)
                                                                                                             gCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGAt                                   >  6:300391/1‑148 (MQ=255)
                                                                                                               ccAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGa                                 >  7:243154/1‑148 (MQ=255)
                                                                                                               ccAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGAc                                <  2:182322/149‑1 (MQ=255)
                                                                                                                cAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTc                                          >  4:225513/1‑138 (MQ=255)
                                                                                                                cAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTc                                          <  3:225513/138‑1 (MQ=255)
                                                                                                                 aaCATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACAt                              <  2:110579/149‑1 (MQ=255)
                                                                                                                               cccGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAgg                <  5:300391/149‑1 (MQ=255)
                                                                                                                                  gCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGt              >  8:229456/1‑148 (MQ=255)
                                                                                                                                  gCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGt              >  1:181746/1‑148 (MQ=255)
                                                                                                                                  gCAAACCGGCATCGGTGCATGGACCGATGACGAGATCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTACCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGt              >  1:44402/1‑148 (MQ=255)
                                                                                                                                        cgtcATCGGTCCATGCACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGAGCATTGGCCGTGACGGCAAGCACTACTACCCGGGGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTAtctc        >  1:80527/4‑149 (MQ=255)
                                                                                                                                         gtcATCGGTCCATGCACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGa      >  5:195076/3‑149 (MQ=255)
                                                                                                                                            aTCGGTCCATGCACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAg   >  6:332497/1‑149 (MQ=255)
                                                                                                                                             tCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGc  <  2:181746/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GCGAATACCTGACGCGCGCTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGC  >  NC_002947/2826679‑2826968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑