Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,826,830 G→C 13.1% W81C (TGG→TGC)  PP_2479 → cytochrome c family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,826,8300GC13.1% 119.2 / 10.5 46W81C (TGG→TGC) PP_2479cytochrome c family protein
Reads supporting (aligned to +/- strand):  ref base G (18/22);  new base C (4/2);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 4.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

TACCTGACGCGCGCTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGC  >  NC_002947/2826684‑2826968
                                                                                                                                                  |                                                                                                                                           
tACCTGACGCGCGCTGCCAACTGTGGCGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTCCATGCAc                                                                                                                                           <  8:293865/149‑1 (MQ=255)
   cTGACGCGCGCTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGa                                                                                                                                        >  1:277179/1‑149 (MQ=255)
     gACGCGCGCTGCCAGCTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGAt                                                                                                                                       >  7:273639/1‑148 (MQ=255)
     gACGCGCGCTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGAt                                                                                                                                       >  7:243305/1‑148 (MQ=255)
          gcgcTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAg                                                                                                                                 >  2:259260/1‑149 (MQ=255)
           cgcTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGt                                                                                                                                <  3:266828/149‑1 (MQ=255)
             cTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTc                                                                                                                              <  1:259260/149‑1 (MQ=255)
             cTGCCAACTGTGTGGCGTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTc                                                                                                                              <  4:71524/149‑1 (MQ=255)
              tGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCg                                                                                                                             >  4:262993/1‑149 (MQ=255)
                ccAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGt                                                                                                                            >  5:165979/1‑148 (MQ=255)
                 cAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGCCGAGTTCGTCa                                                                                                                          <  8:235756/149‑1 (MQ=255)
                 cAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCa                                                                                                                          <  3:90946/149‑1 (MQ=255)
                                ccATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCg                                                                                                           >  6:265827/1‑149 (MQ=255)
                                 cATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTgcgc                                                                                                                    <  6:296401/139‑1 (MQ=255)
                                 cATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTgcgc                                                                                                                    >  5:296401/1‑139 (MQ=255)
                                     ccGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGt                                                                                                                            >  1:273369/1‑127 (MQ=255)
                                     ccGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGt                                                                                                                            <  2:273369/127‑1 (MQ=255)
                                                    aaCCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAg                                                                                      <  2:277179/149‑1 (MQ=255)
                                                    aaCCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTCCATGCACCGATgc                                                                                                                                     <  6:245667/103‑2 (MQ=255)
                                                    aaCCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTCCATGCACCGATgc                                                                                                                                     >  5:245667/1‑102 (MQ=255)
                                                       ccTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGTCCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCac                                                                                   <  8:243305/149‑1 (MQ=255)
                                                           cGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCacta                                                                                 <  8:273639/147‑1 (MQ=255)
                                                           cGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCacta                                                                                 <  2:323968/147‑1 (MQ=255)
                                                                gtggtgTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCg                                                                          <  6:165979/149‑1 (MQ=255)
                                                                      ttGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGttt                                                                    >  1:182322/1‑149 (MQ=255)
                                                                      ttGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGttt                                                                    >  2:268609/1‑149 (MQ=255)
                                                                         aGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTccc                                                                 <  5:265827/149‑1 (MQ=255)
                                                                         aGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTcc                                                                  <  3:262993/148‑1 (MQ=255)
                                                                                    ccGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTa                                                      <  6:55529/149‑1 (MQ=255)
                                                                                     cGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTAc                                                     <  3:190094/149‑1 (MQ=255)
                                                                                           gCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCaaa                                               <  3:222775/149‑1 (MQ=255)
                                                                                                      tCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATATCGCGCGa                                    >  1:110579/1‑149 (MQ=255)
                                                                                                        gCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGAt                                   >  6:300391/1‑148 (MQ=255)
                                                                                                          ccAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGa                                 >  7:243154/1‑148 (MQ=255)
                                                                                                          ccAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGAc                                <  2:182322/149‑1 (MQ=255)
                                                                                                           cAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTc                                          >  4:225513/1‑138 (MQ=255)
                                                                                                           cAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTc                                          <  3:225513/138‑1 (MQ=255)
                                                                                                            aaCATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACAt                              <  2:110579/149‑1 (MQ=255)
                                                                                                                          cccGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAgg                <  5:300391/149‑1 (MQ=255)
                                                                                                                             gCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGt              >  8:229456/1‑148 (MQ=255)
                                                                                                                             gCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGt              >  1:181746/1‑148 (MQ=255)
                                                                                                                             gCAAACCGGCATCGGTGCATGGACCGATGACGAGATCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTACCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGt              >  1:44402/1‑148 (MQ=255)
                                                                                                                                   cgtcATCGGTCCATGCACCGATGACGAGTTCGTCAGTGCGCTGCAGACCGAGCATTGGCCGTGACGGCAAGCACTACTACCCGGGGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTAtctc        >  1:80527/4‑149 (MQ=255)
                                                                                                                                    gtcATCGGTCCATGCACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGa      >  5:195076/3‑149 (MQ=255)
                                                                                                                                       aTCGGTCCATGCACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAg   >  6:332497/1‑149 (MQ=255)
                                                                                                                                        tCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGc  <  2:181746/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                           
TACCTGACGCGCGCTGCCAACTGTGTGGCTTGCCATACCGTGCCTGGCGGTCAACCCTTCGCCGGTGGTGTTGAGTTCAAGCTGCCGTTCGGCTCGCTGTTTTCGCCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGCAGACCG‑GCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGC  >  NC_002947/2826684‑2826968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑