Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,873,758 C→G 8.5% intergenic (+64/+18) cigR → / ← PP_2527 membrane protein/membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,873,7580CG 8.5% 134.2 / 4.0 47intergenic (+64/+18)cigR/PP_2527membrane protein/membrane protein
Reads supporting (aligned to +/- strand):  ref base C (21/22);  new base G (2/2);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01

GTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACACCGCGGCCAGCCCAAGGGTCAGCACCACAGCGCACAGCATGGCCAGCCCGATGGTCACGCCAACGCCCCACGTTTCCAGCAGCCCTGCCAATACCAGATAGAACGCAATCACC  >  NC_002947/2873610‑2873905
                                                                                                                                                    |                                                                                                                                                   
gTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTgcgc                                                                                                                                                     <  5:165433/149‑1 (MQ=255)
        aggCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGcc                                                                                                                                              <  8:133864/146‑1 (MQ=255)
        aaGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCgcagca                                                                                                                                                 <  4:90972/145‑1 (MQ=255)
        aaGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCgcagca                                                                                                                                                 >  3:90972/1‑145 (MQ=255)
        aaGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGccc                                                                                                                                             >  7:133864/1‑149 (MQ=255)
                gcAGACCTGATTCTGGTCGCCGTGGCGACCGGGATGATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCt                                                                                                                                     <  7:295555/149‑1 (MQ=255)
                gcAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGcc                                                                                                                                      >  5:168986/1‑148 (MQ=255)
                 cAGACCTTATTCTGGTCGCGGTGGCTACCGGTATCATGTATGAGGTGTTTAACGGGGCATTCGATTAAGGCCGCAGGTGCGAGCAGTCCGCGCGGTCCCTGCTGGCGCTGGCGTGCCTGCTATGGGCTGCGCAGCAGCCCAACGGCCTa                                                                                                                                    <  5:178524/149‑1 (MQ=255)
                   gACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATc                                                                                                                                  <  3:176743/149‑1 (MQ=255)
                     ccTGATTCTGGTCGCCGTGGAGACCGTGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGAAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGTCGTGCCTGCGATTGGCTGCGCAGCAGCCCAACGGCCTATCaa                                                                                                                                <  2:242147/149‑1 (MQ=255)
                      cTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAAc                                                                                                                               >  6:137507/1‑149 (MQ=255)
                              ggTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACg                                                                                                                                         >  8:260901/1‑131 (MQ=255)
                              ggTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACg                                                                                                                                         <  7:260901/131‑1 (MQ=255)
                                tCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGACCAACGGCCTATCAACGCTGCCGTaa                                                                                                                     >  6:4888/1‑149 (MQ=255)
                                    cGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACAcc                                                                                                                 >  1:151305/1‑149 (MQ=255)
                                                   catcTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACACCGCGGCCAGCCCAAgg                                                                                                  >  7:296196/1‑149 (MQ=255)
                                                    atcTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACACCGCGGCCAGCCCAAgg                                                                                                  <  6:168986/148‑1 (MQ=255)
                                                     tcTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCaa                                                                                                                                >  3:101907/1‑117 (MQ=255)
                                                     tcTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCaa                                                                                                                                <  4:101907/117‑1 (MQ=255)
                                                        aTGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCagcagc                                                                                                                                               >  7:220472/1‑99 (MQ=255)
                                                        aTGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCagcagc                                                                                                                                               <  8:220472/99‑1 (MQ=255)
                                                         tGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACgg                                                                                                                                        >  6:236465/1‑105 (MQ=255)
                                                         tGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACgg                                                                                                                                        <  5:236465/105‑1 (MQ=255)
                                                             gtgtTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACACCGCGGCCAGCCCAAGGGTCAGcacca                                                                                        >  3:152004/1‑149 (MQ=255)
                                                                           aTTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGGCCAACGGCCTATCAACGCTGCCGTAACACCGCGGCCAGCCCAAgg                                                                                                  <  1:226102/125‑1 (MQ=255)
                                                                           aTTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACACCGCGGCCAGCCCAAgg                                                                                                  >  2:226102/1‑125 (MQ=255)
                                                                             tCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACACCGCGGCCAGCCCAAGGGTCAGCACCACAGCGCACAGCATGGc                                                                        >  1:185164/1‑149 (MQ=255)
                                                                              cGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACACCGCGGCCAGCCCAAGGGTCAGCACCACAGCGCACAGCATGGc                                                                        <  5:137507/148‑1 (MQ=255)
                                                                               gACTATGGCCGCTGGTGCGAGCAGGCGGCTCGGTCCCTGCAGGGGGTGGCGTGCCTGCTATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACACCGCGGCCAGCCCAAGGGTCAGCACCACAGCGCACAGCATGGcc                                                                       <  4:152004/148‑1 (MQ=255)
                                                                               gACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACACCGCGGc                                                                                                            <  4:65433/111‑1 (MQ=255)
                                                                               gACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACACCGCGGc                                                                                                            >  3:65433/1‑111 (MQ=255)
                                                                                aCTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAAc                                                                                                                                          <  6:214347/80‑1 (MQ=255)
                                                                                             gTGCTAGCTGTCCGCTGGGTCCCTGCTGGCGCGGGGGTGCCTGCTTTGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGt                                                                                                                       <  4:13883/86‑1 (MQ=255)
                                                                                             gTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGt                                                                                                                       >  3:13883/1‑86 (MQ=255)
                                                                                             gTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACACCGCGGCCAGCCCAAGGGTCAGCACCACAGCGCACAGCATGGCCAGCCCGATGGTCACg                                                        >  1:184382/1‑149 (MQ=255)
                                                                                                 gAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACACCGCGGCCAGCCCAAGGGTCAGCACCACAGCGCACAGCATGGCCAGCCCGATGGTCACGCCaa                                                    >  4:92882/1‑149 (MQ=255)
                                                                                                    cAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACACCGCGGCCAGCCCAAGGGTCAGCACCACAGCGCACAGCATGGCCAGCCCGATGGTCACGCCAACGc                                                 <  3:92882/149‑1 (MQ=255)
                                                                                                         cGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACACCGCGGCCAGCCCAAGGGTCAGCACCACAGCGCACAGCATGGCCAGCCCGATGGTCACGCCAACGCCCCAc                                            <  2:185164/149‑1 (MQ=255)
                                                                                                           cTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACACCGCGGCCAGCCCAAGGGTCAGCACCACAGCGCACAGCATGGCCAGCCCGATGGTCACGCCAACGCCCCACGt                                          >  4:28272/1‑149 (MQ=255)
                                                                                                                tCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACACCGCGGCCAGCCCAAGGGTCAGCACCACAGCGCACAGCATGGCCAGCCCGATGGTCACGCCAACGCCCCACGTTTCca                                     <  8:296196/149‑1 (MQ=255)
                                                                                                                  ccTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACACCGCGGCCAGCCCAAGGGTCAGCACCACAGCGCACAGCATGGCCAGCCCGATGGTCACGCCAACACCCCACGTTTCcag                                    >  4:155041/1‑148 (MQ=255)
                                                                                                                                   gCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACACCGCGGCCAGCCCAAGGGTCAGCACCACAGCGCACAGCATGGCCAGCCCGATGGTCACGCCAACGCCCCACGTTTCCAGCAGCCCTGCCAATAc                     >  7:137236/1‑146 (MQ=255)
                                                                                                                                    cgggctaTGGGCGGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACACCGCGGCCAGCCCAAGGGTCAGCACCACAGCGCACAGCATGGCCAGCCCGATGGTCACGCCAACGCCCCACGTTTCCAGCAGCCCTGCCAATACCAGa                 <  1:154273/143‑1 (MQ=255)
                                                                                                                                      tGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACACCGCGGCCAGCCCAAGGGTCAGCACCACAGCGCACAGCATGGCCAGCCCGATGGTCACGCCAACGCCCCACGTTTCCAGCAGCCCTGCCAATACCAGATa               <  8:137236/149‑1 (MQ=255)
                                                                                                                                        cgttGGGCTGCTGCGCAGCCCATCGGCCTATCAACGCTGCCGTAACACCGCGGCCAGCCCAAGGGTCAGCACCACAGCGCACAGCATGGCCAGCCCGATGGTCACGCCAACGCCCCACGTTTCCAGCAGCCCTGCCAATACCa                   >  6:146546/4‑143 (MQ=255)
                                                                                                                                        cgttGGGCTGCTGCGCAGCCCATCGGCCTATCAACGCTGCCGTAACACCGCGGCCAGCCCAAGGGTCAGCACCACAGCGCACAGCATGGCCAGCCCGATGGTCACGCCAACGCCCCACGTTTCCAGCAGCCCTGCCAATACCa                   <  5:146546/140‑1 (MQ=255)
                                                                                                                                        cgttGGGCTGCTGCGCAGCCCATCGGCCTATCAACGCTGCCGTAACACCGCGGCCAGCCCAAGGGTCAGCACCACAGCGCACAGCATGGCCAGCCCGATGGTCACGCCAACGCCCCACGTTTCCAGCAGCCCTGCCAATACCAGATAg              >  8:162054/4‑148 (MQ=255)
                                                                                                                                        cGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACACCGCGGCCAGCCCAAGGGTCAGCACCACAGCGCACAGCATGGCCAGCCCGATGGTCACGCCAACACCCCACGTTTCCAGCAGCCCTGCCAATACCAGATa               <  3:155041/147‑1 (MQ=255)
                                                                                                                                          ttGGGCTGCTGCGCAGCCCATCGGCCTATCAACGCTGCCGTAACACCGCGGCCAGCCCAAGGGTCAGCACCACAGCGCACAGCATGGCCAGCCCGATGGTCACGCCAACGCCCCACGTTTCCAGCAGCCCTGCCAATACCAGATAGAAc           <  7:162054/148‑1 (MQ=255)
                                                                                                                                                tGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACACCGCGGCCAGCCCAAGGGTCAGCACCACAGCGCACAGCATGGCCAGCCCGATGGTCACGCCAACGCCCCACGTTTCCAGCAGCCCTGCCAATACCAGATAGAACGCAAt      >  2:164220/1‑148 (MQ=255)
                                                                                                                                                   gcagcaGCCCAACGGCCTATCAACGCTGCCGTAACACCGCGGCCAGCCCAAGGGTCAGCACCACAGCGCACAGCATGGCCAGCCCGATGGTCACGCCAACGCCCCACGTTTCCAGCAGCCCTGCCAATACCAGATAGAACGCAATCAcc  <  3:28272/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACACCGCGGCCAGCCCAAGGGTCAGCACCACAGCGCACAGCATGGCCAGCCCGATGGTCACGCCAACGCCCCACGTTTCCAGCAGCCCTGCCAATACCAGATAGAACGCAATCACC  >  NC_002947/2873610‑2873905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑