Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,936,413 C→G 7.6% Y149* (TAC→TAG)  PP_2569 → MHS family metabolite MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,936,4130CG 7.6% 148.0 / 4.4 53Y149* (TAC→TAG) PP_2569MHS family metabolite MFS transporter
Reads supporting (aligned to +/- strand):  ref base C (24/24);  new base G (2/2);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.35e-01

CCATTGTCATCATGGGCCTGTCCACCGCAGTGGTGGGGCTGCTACCGGGCTACGCCACGATCGGTGTGGCCGCGCCGATCATCCTGATCACCCTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCACCCTGGGGCTGTTCCTGTCGCTGCTGGTGATCATGGCCTGTCGCACGGCCATGGGGACGGAAGCGTTCGAGGCCTGGGGCT  >  NC_002947/2936265‑2936558
                                                                                                                                                    |                                                                                                                                                 
ccATTGTCCTCATCGGCCTGTCCACCGCAGTGGGGGGGCTGCTACCGGGCTACGCCACGATCGGTGTGGCCGCGCCGATCATCCAGATCACCCTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTAc                                                                                                                                                   <  3:251517/149‑1 (MQ=255)
 cATTGTCATCATGGGCCTGTCCACCGCAGTGGTGGGGCTGCTACCGGGCTACGCCACGATCGGTGTGGCCGCGCCGATCATCCTGATCACCCTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACg                                                                                                                                                  >  3:92857/1‑149 (MQ=255)
  aTTGTCATCATGGGCCTGTCCACCGCAGTGGTGGGGCTGCTACCGGGCTACGCCACGATCGGTGTGGCCGCGCCGATCATCCTGATCACCCTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGt                                                                                                                                                 <  1:321288/149‑1 (MQ=255)
        atcatGGGCCTGTCCACCGCAGTGGTGGGGCTGCTACCGGGCTACGCCACGATCGGTGTGGCCGCGCCGATCATCCTGATCACCCTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGa                                                                                                                                           >  5:41064/1‑149 (MQ=255)
         tcatGGGCCTGTCCACCGCAGTGGTGGGGCTGCTACCGGGCTACGCCACGATCGGTGTGGCCGCGCCGATCATCCTGATCACCCTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACGTAGGTGGCCgcg                                                                                                                                          <  4:248234/149‑3 (MQ=255)
           atGGGCCTGTCCACCGCAGTGGTGGGGCTGCTACCGGGCTACGCCACGATCGGTGTGGCCGCGCCGATCATCCTGATCACCCTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGc                                                                                                                                         <  6:321039/148‑1 (MQ=255)
             gggCCTGTCCACCGCAGTGGTGGGGCTGCTACCGGGCTACGCCACGATCGGTGTGGCCGCGCCGATCATCCTGATCACCCTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATg                                                                                                                                      >  2:288820/1‑149 (MQ=255)
                      cACCGCAGTGGTGGGGCTGCTACCGGGCTACGCCACGATCGGTGTGGCCGCGCCGATCATCCTGATCACCCTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATGCGCCCAAgg                                                                                                                             <  4:142264/149‑1 (MQ=255)
                       aCCGCAGTGGTGGGGCTGCTACCGGGCTACGCCACGATCGGTGTGGCCGCGCCGATCATCCTGATCACCCTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATGCGCCCAAggg                                                                                                                            <  4:92857/149‑1 (MQ=255)
                             gtggtgGGGCTGCTACCGGGCTACGCCACGATCGGTGTGGCCGCGCCGATCATCCTGATCACCCTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCCCCGACGTGGCCGAGCAAgcgc                                                                                                                                   >  2:114083/1‑136 (MQ=255)
                             gtggtgGGGCTGCTACCGGGCTACGCCACGATCGGTGTGGCCGCGCCGATCATCCTGATCACCCTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATgcgc                                                                                                                                   <  1:114083/136‑1 (MQ=255)
                             gtggtgGGGCTGCTACCGGGCTACGCCACGATCGGTGTGGCCGCGCCGATCATCCTGATCACCCTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATGCGCCCAAGGGccgccg                                                                                                                      <  1:288820/149‑1 (MQ=255)
                                   gggCTGCTACCGGGCTACGCCACGATCGGTGTGGCCGCGCCGATCATCCTGATCACCCTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCtt                                                                                                                >  8:226243/1‑149 (MQ=255)
                                    ggCTGCTACCGGGCTACGCCACGATCGGTGTGGCCGCGCCGATCATCCTGATCACCCTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCttt                                                                                                               <  7:226243/149‑1 (MQ=255)
                                      ctgctACCGGGCTACGCCACGATCGGTGTGGCCGCGCCGATCATCCTGATCACCCTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCttttt                                                                                                             <  4:63004/149‑1 (MQ=255)
                                         ctACCGGGCTACGCCACGATCGGTGTGGCCGCGCCGATCATCCTGATCACCCTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCAc                                                                                                          >  7:230646/1‑149 (MQ=255)
                                            ccGGGCTACGCCACGATCGGTGTGGCCGCGCCGATCATCCTGATCACCCTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCGTTTTCACTgg                                                                                                       >  3:134626/1‑148 (MQ=255)
                                                    cGCCACGATCGGTGTGGCCGCGCCGATCATCCTGATCACCCTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATc                                                                                                <  6:41064/148‑1 (MQ=255)
                                                     gCCACGATCGGTGTGGCCGCGCCGATCATCCTGATCACCCTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCCCCTAACTTGCCCAGCAATCGCCCAAGGGCCGCCCCGGCGTTTTGACTGCCAGGAtac                                                                                               >  4:16962/1‑146 (MQ=255)
                                                        aCGATCGGTGTGGCCGCGCCGATCATCCTGATCACCCTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAgac                                                                                           <  7:181718/149‑1 (MQ=255)
                                                           aTCGGTGTGGCCGCGCCGATCATCCTGATCACCCTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAgacgac                                                                                        <  3:16962/149‑1 (MQ=255)
                                                                   ggCCGCGCCGATCATCCTGATCACCCTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCAccc                                                                                >  4:240671/1‑149 (MQ=255)
                                                                    gCCGCGCCGATCATCCTGATCACCCTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCACCCt                                                                               >  2:246785/1‑149 (MQ=255)
                                                                    gCCGCGCCGATCATCCTGATCACCCTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCACCCt                                                                               >  1:31144/1‑149 (MQ=255)
                                                                      cgcgCCGATCATCCTGATCACCCTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCACCCTgg                                                                             >  6:66748/1‑149 (MQ=255)
                                                                        cgcCGAGCATCCTGCTCACCCTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGGGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCAGGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCACCCTgggg                                                                           <  5:93676/149‑1 (MQ=255)
                                                                          ccGATCATCCTGATCACCGTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCACCCTGGGGCt                                                                         <  5:319444/149‑1 (MQ=255)
                                                                            gATCATCCTGATCACCCTGCGCTTGCTGGAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCACCCTGGGGCTGt                                                                       >  1:180647/1‑149 (MQ=255)
                                                                            gATCATCCTGATCACCCTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCACCCTGGGGCTGt                                                                       <  2:180647/149‑1 (MQ=255)
                                                                            gATCATCCTGATCACCCTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCACCCTGGGGCTGt                                                                       <  2:31144/149‑1 (MQ=255)
                                                                                                    gctgcAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCACCCTGGGGCTGTTCCTGTCGCTGCTGGTGATCATgg                                               >  7:10900/1‑149 (MQ=255)
                                                                                                     ctgcAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCACCCTGGGGCTGTTCCTGTCGCTGCTGGTGATCATGGc                                              <  7:279313/149‑1 (MQ=255)
                                                                                                      tgcAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCACCCTGGGGCTGTTCCTGTCGCTGCTGGTGa                                                     >  4:190947/1‑141 (MQ=255)
                                                                                                      tgcAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCACCCTGGGGCTGTTCCTGTCGCTGCTGGTGa                                                     <  3:190947/141‑1 (MQ=255)
                                                                                                           gggCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCCGACGACTGCCACCCTGGGGCTGTTCCTGTCGCTGCTGGTGATCATGGCCTGTCg                                        <  1:246785/149‑1 (MQ=255)
                                                                                                                ggCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCCCCCTGGGGCTg                                                                        >  6:264892/1‑112 (MQ=255)
                                                                                                                  cgcTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGCCGACTGCCACCCTGGGGCTGTTCCTGTCGCTGCTGGTGATCATGGCCTGTCGCACGGc                                  >  5:126773/1‑148 (MQ=255)
                                                                                                                      gggCGGTGAGTACGGCGGCGCGGCCACCAACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCATTTTCACTGCCTGGATCCAGACGACTGCCACCCTGGGGCTGTTCCTGTCGCTGCTGGTGATCATGGCCTGTCGCACGGCCATgg                             >  6:127459/1‑149 (MQ=255)
                                                                                                                       ggCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCACCCTGGGGCTGTTCCTGTCGCTGCTGGTGATCATGGCCTGTCGCACGGCCATggg                            <  5:51169/149‑1 (MQ=255)
                                                                                                                         cGGTGAGTACGGCGGCGCGGGCCCCGACGTGGCCGCGCCTGCGCCCCAGGGCCGCCGCGGGGTTTTGCCTGCCTGGATGCAGACGCCGGGCCCCCGGGGGGGGGTGCTGTGGGTGGTGGTGATCATGGCCTGTAGCCCGGGCATggggc                          >  8:125755/1‑148 (MQ=255)
                                                                                                                              aGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCACCCTGGGGCTGTTCCTGTCGCTGCTGGTGATCATGGCCTGTCGCACGGCCATGGGGACGGaa                     <  6:126773/149‑1 (MQ=255)
                                                                                                                                     atgctcggcCACGTAGGTGGCCGCGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCACCCTGGGGCTGTTCCTGTCGCTGCTGGTGATCATgg                                               >  6:28718/6‑116 (MQ=255)
                                                                                                                                     atgctcggcCACGTAGGTGGCCGCGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCACCCTGGGGCTGTTCCTGTCGCTGCTGGTGATCATgg                                               <  5:28718/111‑1 (MQ=255)
                                                                                                                                      ggcgcggcCACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCACCCTGGGGCTGTTCCTGTCGctgctg                                                         <  1:175569/105‑1 (MQ=255)
                                                                                                                                      ggcgcggcCACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCACCCTGGGGCTGTTCCTGTCGctgctg                                                         >  2:175569/1‑105 (MQ=255)
                                                                                                                                       gctcggcCACGTAGGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCACCCTGGGGCTGTTCCTGTCGCTGCTGGTGATCATGGCCTGTCGCACGGCCATGGGGACGGAAGCGTTCGAg            >  3:36859/4‑149 (MQ=255)
                                                                                                                                              cACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCACCCTGGGGCTGTTCCTGTCGCTGCTGGTGATCATGGCCTGTCGCACgg                                   >  6:24976/1‑119 (MQ=255)
                                                                                                                                              cACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCACCCTGGGGCTGTTCCTGTCGCTGCTGGTGATCATGGCCTGTCGCACgg                                   <  5:24976/119‑1 (MQ=255)
                                                                                                                                              cACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCACCCTGGGGCTGTTCCTGTCGCTGCTGGTGATCATGGCCTGTCGCACGGCCATGGGGACGGAAGCGTTCGAGGCCTggg     >  7:186937/1‑149 (MQ=255)
                                                                                                                                                 cTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCACCCTGGGGCTGTTCCTGTCGCTGCTGGTGATCATGGCCTGTCGCACGGCCATGTGGACGGAAGCGTTCGAGGCCTGGGGCt  <  6:240392/149‑1 (MQ=255)
                                                                                                                                                 cTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCACCCTGGGGCTGTTCCTGTCGCTGCTGGTGATCATGGCCTGTCGCACGGCCATGGGGACGGAAGCGTTCGAGGCCTGGGGCt  >  1:238971/1‑149 (MQ=255)
                                                                                                                                                 cTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCACCCTGGGGCTGTTCCTGTCGCTGCTGGTGATCATGGCCTGTCGCACGGCCATGGGGACGGAAGCGTTCGAGGCCTGGGGCt  >  5:77785/1‑149 (MQ=255)
                                                                                                                                                   aCGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCACCCTGGGGCTGTTCCTGTCGCTGCTGGTGa                                                     <  5:318211/96‑1 (MQ=255)
                                                                                                                                                   aCGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCACCCTGGGGCTGTTCCTGTCGCTGCTGGTGa                                                     >  6:318211/1‑96 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CCATTGTCATCATGGGCCTGTCCACCGCAGTGGTGGGGCTGCTACCGGGCTACGCCACGATCGGTGTGGCCGCGCCGATCATCCTGATCACCCTGCGCTTGCTGCAGGGGCTGGCGCTGGGCGGTGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCATGCGCCCAAGGGCCGCCGCGGCTTTTTCACTGCCTGGATCCAGACGACTGCCACCCTGGGGCTGTTCCTGTCGCTGCTGGTGATCATGGCCTGTCGCACGGCCATGGGGACGGAAGCGTTCGAGGCCTGGGGCT  >  NC_002947/2936265‑2936558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑