Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,939,825 A→G 7.5% L106P (CTG→CCG) ‡ PP_2572 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,939,8250AG 7.5% 151.0 / 3.0 53L106P (CTG→CCG) ‡PP_2572hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (25/24);  new base G (2/2);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

ACCTGGTAACGCGCCATCAGGTCACCCAGGGTCTTGGGCGTGAAGTGCTGCAGGATGCGCCAGGTGTACAGCATCAGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAGGGCAGGTTGTCGACACCGGCCAGCGGCAGCACGATGCCTTCGCGCACCGCCTGGTCGCCGCTGTCACCGTGGGTGAACGCACCGGCCAGGTCGTCGGGCAG  >  NC_002947/2939681‑2939967
                                                                                                                                                |                                                                                                                                              
aCCTGGTAACGCGCCATCAGGTCACCCAGGGTCATGGGCGTGAAGTGCTGCAGGATGCGCCACGTGTACAGCATCAGCGCACCGTGCACGCCCCGCAGTACATAGCCATTCCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGc                                                                                                                                            >  6:72979/1‑149 (MQ=255)
     gTAACGCGCCATCAGGTCACCCAGGGTCGTGGGCGTGAAGTGCTGCAGGATGCGCCAGGTGTACAGCATCAGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCggg                                                                                                                                        <  3:64580/148‑1 (MQ=255)
        aCGCGCCATCAGGTCACCCAGGGTCTTGGGCGTGAAGTGCTGCAGGATGCGCCAGGTGTACAGCATCAGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCggg                                                                                                                                        >  8:85121/1‑145 (MQ=255)
        aCGCGCCATCAGGTCACCCAGGGTCTTGGGCGTGAAGTGCTGCAGGATGCGCCAGGTGTACAGCATCAGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCggg                                                                                                                                        <  7:85121/145‑1 (MQ=255)
         cgcgCCATCAGGTCACCCAGGGTCTTGGGCGTGAAGTGCTGCAGGATGCGCCAGGTGTACAGCATCAGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGtgtg                                                                                                                                   <  1:69842/149‑1 (MQ=255)
         cgcgCCATCAGGTCACCCAGGGTCTTGGGCGTGAAGTGCTGCAGGATGCGCCAGGTGTACAGCATCAGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGtgtg                                                                                                                                   <  4:195477/149‑1 (MQ=255)
                    gTCACCCAGGGTCTTGGGCGTGAAGTGCTGCAGGATGCGCCAGGTGTACAGCATCAGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAggg                                                                                                                        <  5:141111/149‑1 (MQ=255)
                     tCACCCAGGGTCTTGGGCGTGAAGTGCTGCAGGATGCGCCAGGTGTACAGCATCAGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGt                                                                                                                       >  8:220815/1‑149 (MQ=255)
                       aCCCAGGGTCTTGGGCGTGAAGTGCTGCAGGATGCGCCAGGTGTACAGCATCAGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTg                                                                                                                      >  2:229571/1‑148 (MQ=255)
                               tCTTGGGCGTGAAGTGCTGCAGGATGCGCCAGGTGTACAGCATCAGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCggg                                                                                                                                        >  8:267718/1‑122 (MQ=255)
                               tCTTGGGCGTGAAGTGCTGCAGGATGCGCCAGGTGTACAGCATCAGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCggg                                                                                                                                        <  7:267718/122‑1 (MQ=255)
                                 ttGGGCGTGAAGTGCTGCAGGATGCGCCAGGTGTACAGCATCAGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGtt                                                                                                                                >  6:15881/1‑128 (MQ=255)
                                 ttGGGCGTGAAGTGCTGCAGGATGCGCCAGGTGTACAGCATCAGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGtt                                                                                                                                <  5:15881/128‑1 (MQ=255)
                                  tGGGCGTGAAGTGCTGCAGGATGCGCCAGGTGTACAGCATCAGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCgg                                                                                                          >  7:251298/1‑149 (MQ=255)
                                    ggCGTGAAGTGCTGCAGGATGCGCCAGGTGTACAGCATCAGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTa                                                                                                        <  1:20562/149‑1 (MQ=255)
                                    ggCGTGAAGTGCTGCAGGATGCGCCAGGTGTACAGCATCAGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTa                                                                                                        <  4:94932/149‑1 (MQ=255)
                                      cGTGAAGTGCTGCAGGATGCACCAGGTGTACAGCATCAGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAgg                                                                                                      <  6:29132/149‑1 (MQ=255)
                                       gTGAAGTGCTGCAGTATTCGCCAGGTGTACAGCATCAGCTCACCGTGCTCGACCCGCAGTACATAGCCTTTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGATCAGGGCCGGGGTGTGGTTATCGAGGTTGAACAGGATGCGGTAggg                                                                                                     <  2:145729/149‑1 (MQ=255)
                                        tGAAGTGCTGCAGGATGCGCCAGGTGTACAGCATCAGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAggg                                                                                                     >  4:146136/1‑148 (MQ=255)
                                             tgctgcAGGATGCGCCAGGTGTACAGCATCAGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAg                                                                                                       <  1:293177/141‑1 (MQ=255)
                                             tgctgcAGGATGCGCCAGGTGTACAGCATCAGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAg                                                                                                       >  2:293177/1‑141 (MQ=255)
                                                tgcAGGATGCGCCAGGTGTACAGCATCAGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAGGGCAGGTTGTc                                                                                            >  6:294200/1‑149 (MQ=255)
                                                 gcAGGATGCGCCAGGTGTACAGCATCAGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAGGGCAGGTTGTc                                                                                            >  6:230059/1‑148 (MQ=255)
                                                 gcAGGATGCGCCAGGTGTACAGCATCAGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAGGGCAGGTTGTc                                                                                            <  5:230059/148‑1 (MQ=255)
                                                  cAGGATGCGCCAGGTGTACAGCATCAGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAGGGCAGGTTGTc                                                                                            <  7:220815/147‑1 (MQ=255)
                                                                    ctgCATCAGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAGGGCAGGTTGTCGACACCGGCCAGCGgcagca                                                                        <  2:118553/147‑1 (MQ=255)
                                                                       cATCAGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAgg                                                                                                      >  8:297772/1‑116 (MQ=255)
                                                                       cATCAGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAGGGCAGGTTg                                                                                              <  2:319889/124‑1 (MQ=255)
                                                                       cATCAGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAGGGCAGGTTg                                                                                              >  1:319889/1‑124 (MQ=255)
                                                                          cAGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAGGGCAGGTTGTCGACACCGGCCAGCGGCAGCACGATGc                                                                  >  1:130977/1‑149 (MQ=255)
                                                                           aGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGACGCGGTAGGGCAGGTTGTCGACACCGGCCAGCGGCAGCACGATGc                                                                  <  1:229571/148‑1 (MQ=255)
                                                                            gcgcACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAGGGCAGGTTGTCGACACCGGCCAGCGGCAGCACCATGCCt                                                                >  3:83112/1‑149 (MQ=255)
                                                                             cgcACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAGGGCAGGTTGTCGACACCGGCCAGCGgcagca                                                                        >  1:273418/1‑140 (MQ=255)
                                                                             cgcACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAGGGCAGGTTGTCGACACCGGCCAGCGgcagca                                                                        <  2:273418/140‑1 (MQ=255)
                                                                                        cGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGATCAGGATGCGGTAGGGCAGGTTGTCGACACCGGCCAGCGGCAGCACGATGCCTTCGCGCACCGcc                                                    >  6:161802/1‑149 (MQ=255)
                                                                                         gACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAGGGCAGGTTGTCGACACCGGCCAGCGGCAGCACGATGCCTTCGCGCACCGcc                                                    <  2:130977/148‑1 (MQ=255)
                                                                                            ccGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAGGGCAGGTTGTCGACACCGGCCAGCGGCAGCACGATGCCTTCGCGCACCGc                                                     <  8:75031/144‑1 (MQ=255)
                                                                                            ccGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAGGGCAGGTTGTCGACACCGGCCAGCGGCAGCACGATGCCTTCGCGCACCGc                                                     >  7:75031/1‑144 (MQ=255)
                                                                                                           aTTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAGGGCAGGtt                                                                                               >  4:213059/1‑87 (MQ=255)
                                                                                                            ttGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAGGGCAGGTTGTCGACACCGGCCAGCGGCAGCACGATGCCTTCGCGCACCGCCTGGTCGCCGCTGTCACCGt                                 <  7:238223/148‑1 (MQ=255)
                                                                                                            ttGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAGGGCAGGTTGTCGACACCGGCCAGCGGCAGCACGATGCCTTCGCGCACCGCCTGGTCGCCGCTGTCACCGt                                 >  8:238223/1‑148 (MQ=255)
                                                                                                               cgcCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAGGGCAGGTTGTCGACACCGGCCAGCGGCAGCACGATGCCTTCGCGCACCGCCTGGTCGCCGCTGTCACCGTGGGt                             >  7:181816/1‑149 (MQ=255)
                                                                                                                   ggTGCTTCAGTCGGCTGCCGTCCTCGCGCAGGGACGGGGTGTGGTTATCGAGGGTGAACAGTATGAGGTAGGTCAGGTTGTCGACACCGGCCATCGGCAGCACGATGCCTTCGCGGACCGCCTGGTGGCAGCTGTCACCGTGGGTGAAc                         <  4:83112/149‑1 (MQ=255)
                                                                                                                              cacaccccGGCCCTGCGCGAGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAGGGCAGGTTGTCGACACCGGCCAGCGGCAGCACGATGCCTTCGCGCACCGCCTGGTCGCCGCTGTCACCGTGGGTGAACGCACCGGCCAg              >  8:82577/7‑149 (MQ=255)
                                                                                                                                caccccGGCCCTGCGCGAGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAGGGCAGGTTGTCGACACCGGCCAGCGGCAGCACGATGCCTTCGCGCACCGCCTGGTCGCCGCTGTCACCGTggg                              <  1:97543/127‑1 (MQ=255)
                                                                                                                                caccccGGCCCTGCGCGAGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAGGGCAGGTTGTCGACACCGGCCAGCGGCAGCACGATGCCTTCGCGCACCGCCTGGTCGCCGCTGTCACCGTggg                              >  2:97543/5‑131 (MQ=255)
                                                                                                                                 accccGGCCCTGCGCGAGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAGGGCAGGTTGTCGACACCGGCCAGCGGCAGCACGATGCCTTCGCGCACCGCCTGGTCGCCGCTGTCACCGTGGGTGAACGCACCGGCCAGgtc           <  7:82577/146‑1 (MQ=255)
                                                                                                                                    ccGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAGGGCAGGTTGTCGACACCGGCCAGCGGCAGCACGATGCCTTCGCGCACCGCCTGGTCGCCGCTGTCACCGTGGGTGAACGCACCGGCCAGgtcgtc        <  8:251298/149‑1 (MQ=255)
                                                                                                                                     cGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAGGGCAGGTTGTCGACACCGGCCAGCGGCAGCACGATGCCTTCGCGCACCGCCTGGTCGCCGCTGTCACCGTGGGTGAACGCACCGGCCAGGtcgtcg       >  3:18634/1‑149 (MQ=255)
                                                                                                                                      ggCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAGGGCAGGTTGTCGACACCGGCCAGCGGCAGCACGATGCCTTCGCGCACCGCCTGGTCGCCGCTGTCACCGTGGGTGAACGCACCGGCCAGGTCGTCgg      >  2:206561/1‑149 (MQ=255)
                                                                                                                                          tCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAGGGCAGGTTGTCGACACCGGCCAGCGGCAGCACGATGCCTTCGCGCACCGCCTGGTCGCCGCTGTCACCGTGGGTGAAc                         >  1:323718/1‑126 (MQ=255)
                                                                                                                                          tCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAGGGCAGGTTGTCGACACCGGCCAGCGGCAGCACGATGCCTTCGCGCACCGCCTGGTCGCCGCTGTCACCGTGGGTGAAc                         <  2:323718/126‑1 (MQ=255)
                                                                                                                                          tCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAGGGCAGGTTGTCGACACCGGCCAGCGGCAGCACGATGCCTTCGCGCACCGCCTGGTCGCCGCTGTCACCGTGGGTGAACGCACCGGCCAGGTCGTCGGGCAg  >  4:278431/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                              
ACCTGGTAACGCGCCATCAGGTCACCCAGGGTCTTGGGCGTGAAGTGCTGCAGGATGCGCCAGGTGTACAGCATCAGCGCACCGTGCTCGACCCGCAGTACATAGCCATTGCGCCGGTGCTTCAGGCGGCTGCCGGCCTCGCGCAGGGCCGGGGTGTGGTTATCGAGGGTGAACAGGATGCGGTAGGGCAGGTTGTCGACACCGGCCAGCGGCAGCACGATGCCTTCGCGCACCGCCTGGTCGCCGCTGTCACCGTGGGTGAACGCACCGGCCAGGTCGTCGGGCAG  >  NC_002947/2939681‑2939967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑