Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,951,341	A→C	19.8%	intergenic (‑120/+41)	oguA ← / ← PP_2585	8‑oxoguanine deaminase/alpha‑ketoglutaric semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,951,341	0	A	C	19.8%	74.7 / 14.2	40	intergenic (‑120/+41)	oguA/PP_2585	8‑oxoguanine deaminase/alpha‑ketoglutaric semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (14/18);  new base C (5/3);  total (19/21)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

TTTTTGCAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAGGGTAGTTCTGGAA  >  NC_002947/2951197‑2951486                                                                                                                                                 |                                                                                                                                                   tttttGCAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGc                                                                                                                                                >  8:22911/1‑149 (MQ=255)     tGCAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCcgc                                                                                                                                             <  3:98189/148‑1 (MQ=255)       cAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTATTCGCGGCTCTAGCcgcca                                                                                                                                           >  2:306967/1‑146 (MQ=255)        aGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCcgcg                                                                                                                                            >  7:21609/1‑146 (MQ=255)        aGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAag                                                                                                                                         <  4:139232/149‑1 (MQ=255)         ggggTTGTAGATGACATGGTGCAAGCATGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTCGCGGCTCTAGCCGCCAaga                                                                                                                                        <  6:268741/149‑1 (MQ=39)         gggTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAaga                                                                                                                                        <  4:265924/149‑1 (MQ=255)            tttGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTCGCGGCTCTAGCCGCCAAGAGGc                                                                                                                                     <  5:4398/149‑1 (MQ=255)              tGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTCGCGGCTCTAGCCGCCAAGAGGcc                                                                                                                                    <  1:306967/148‑1 (MQ=255)                       cATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGtt                                                                                                                          >  5:79853/1‑149 (MQ=255)                        aTGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTg                                                                                                                         >  2:45775/1‑149 (MQ=255)                        aTGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTg                                                                                                                         <  6:79853/149‑1 (MQ=255)                           gTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACCGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑Ca                                                                                                                      <  7:120/148‑1 (MQ=255)                                   cTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGaaa                                                                                                             <  7:22911/149‑1 (MQ=255)                                      cgcAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGa                                                                                                           <  1:45775/148‑1 (MQ=255)                                                    gCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTcgcg                                                                                            >  1:80610/1‑149 (MQ=255)                                                    gCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTcgcg                                                                                            <  1:200976/149‑1 (MQ=255)                                                      gATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGgc                                                                                          <  6:190382/149‑1 (MQ=255)                                                               tGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCacac                                                                                 >  2:48728/1‑149 (MQ=255)                                                                 tCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACAcc                                                                                >  5:40515/1‑148 (MQ=255)                                                                   aTAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑c                                                                                                                       >  5:72179/1‑107 (MQ=255)                                                                   aTAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑c                                                                                                                       <  6:72179/107‑1 (MQ=255)                                                                         gcTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCaa                                                                       >  2:93952/1‑149 (MQ=255)                                                                                    tAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATc                                                             <  1:93952/148‑1 (MQ=255)                                                                                    tAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATc                                                             <  6:40515/148‑1 (MQ=255)                                                                                     aagcaagcTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGACAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATc                                                             <  1:253718/148‑1 (MQ=255)                                                                                             tttttACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGACGCGAAGAGGCAGGCACGGGTTG‑CAGCCATGAAAGATAACAACGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGt                                                   >  7:22257/1‑149 (MQ=255)                                                                                                      aTTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGtttt                                          >  2:308255/1‑149 (MQ=255)                                                                                                          tataCAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCaa                                      >  8:175336/1‑149 (MQ=255)                                                                                                            taCAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAAc                                     <  8:21609/148‑1 (MQ=255)                                                                                                                 cTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATcc                               <  7:175336/149‑1 (MQ=255)                                                                                                                    gAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCgg                             >  5:176977/1‑148 (MQ=255)                                                                                                                     aaGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCgg                             >  3:107571/1‑147 (MQ=255)                                                                                                                                ccGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGTCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCTGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTc                 <  8:22257/148‑1 (MQ=255)                                                                                                                                   gCCTCTTCGCGGCTCTAGCCGCCAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAggg             >  5:122186/1‑149 (MQ=255)                                                                                                                                   gCCTCTTCGCGGCTCTAGCCGCCAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTGTTCAACGCATCCGGCAGCAGTGCGTCAggg             >  3:197903/1‑149 (MQ=39)                                                                                                                                     ctctTCGCGGCTCTAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAggg             >  5:150992/1‑147 (MQ=255)                                                                                                                                     ctctTCGCGGCTCTAGCCGCCAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCACCGCATCCGGCAGCAGTGCGTCAggg             >  8:180241/1‑147 (MQ=39)                                                                                                                                          ggcggcTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAGGGTAGtt        <  6:176977/147‑1 (MQ=255)                                                                                                                                              gcTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAGGGTAGTTCTGGaa  <  2:80610/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                   TTTTTGCAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAGGGTAGTTCTGGAA  >  NC_002947/2951197‑2951486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑