Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,951,349 G→C 18.0% intergenic (‑128/+33) oguA ← / ← PP_2585 8‑oxoguanine deaminase/alpha‑ketoglutaric semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,951,3490GC18.0% 86.8 / 14.0 39intergenic (‑128/+33)oguA/PP_25858‑oxoguanine deaminase/alpha‑ketoglutaric semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (14/18);  new base C (4/3);  total (18/21)
Fisher's exact test for biased strand distribution p-value = 6.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.31e-01

AGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAGGGTAGTTCTGGAAGCACACCGGGC  >  NC_002947/2951204‑2951497
                                                                                                                                                 |                                                                                                                                                     
aGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCcgcg                                                                                                                                                       >  7:21609/1‑146 (MQ=255)
aGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAag                                                                                                                                                    <  4:139232/149‑1 (MQ=255)
 ggggTTGTAGATGACATGGTGCAAGCATGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTCGCGGCTCTAGCCGCCAaga                                                                                                                                                   <  6:268741/149‑1 (MQ=39)
 gggTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAaga                                                                                                                                                   <  4:265924/149‑1 (MQ=255)
    tttGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTCGCGGCTCTAGCCGCCAAGAGGc                                                                                                                                                <  5:4398/149‑1 (MQ=255)
      tGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTCGCGGCTCTAGCCGCCAAGAGGcc                                                                                                                                               <  1:306967/148‑1 (MQ=255)
               cATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGtt                                                                                                                                     >  5:79853/1‑149 (MQ=255)
                aTGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTg                                                                                                                                    <  6:79853/149‑1 (MQ=255)
                aTGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTg                                                                                                                                    >  2:45775/1‑149 (MQ=255)
                   gTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACCGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑Ca                                                                                                                                 <  7:120/148‑1 (MQ=255)
                           cTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGaaa                                                                                                                        <  7:22911/149‑1 (MQ=255)
                              cgcAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGa                                                                                                                      <  1:45775/148‑1 (MQ=255)
                                            gCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTcgcg                                                                                                       <  1:200976/149‑1 (MQ=255)
                                            gCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTcgcg                                                                                                       >  1:80610/1‑149 (MQ=255)
                                              gATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGgc                                                                                                     <  6:190382/149‑1 (MQ=255)
                                                       tGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCacac                                                                                            >  2:48728/1‑149 (MQ=255)
                                                         tCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACAcc                                                                                           >  5:40515/1‑148 (MQ=255)
                                                           aTAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑c                                                                                                                                  >  5:72179/1‑107 (MQ=255)
                                                           aTAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑c                                                                                                                                  <  6:72179/107‑1 (MQ=255)
                                                                 gcTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCaa                                                                                  >  2:93952/1‑149 (MQ=255)
                                                                            tAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATc                                                                        <  1:93952/148‑1 (MQ=255)
                                                                            tAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATc                                                                        <  6:40515/148‑1 (MQ=255)
                                                                             aagcaagcTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGACAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATc                                                                        <  1:253718/148‑1 (MQ=255)
                                                                                     tttttACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGACGCGAAGAGGCAGGCACGGGTTG‑CAGCCATGAAAGATAACAACGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGt                                                              >  7:22257/1‑149 (MQ=255)
                                                                                              aTTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGtttt                                                     >  2:308255/1‑149 (MQ=255)
                                                                                                  tataCAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCaa                                                 >  8:175336/1‑149 (MQ=255)
                                                                                                    taCAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAAc                                                <  8:21609/148‑1 (MQ=255)
                                                                                                         cTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATcc                                          <  7:175336/149‑1 (MQ=255)
                                                                                                            gAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCgg                                        >  5:176977/1‑148 (MQ=255)
                                                                                                             aaGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCgg                                        >  3:107571/1‑147 (MQ=255)
                                                                                                                        ccGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGTCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCTGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTc                            <  8:22257/148‑1 (MQ=255)
                                                                                                                           gCCTCTTCGCGGCTCTAGCCGCCAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAggg                        >  5:122186/1‑149 (MQ=255)
                                                                                                                           gCCTCTTCGCGGCTCTAGCCGCCAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTGTTCAACGCATCCGGCAGCAGTGCGTCAggg                        >  3:197903/1‑149 (MQ=39)
                                                                                                                             ctctTCGCGGCTCTAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAggg                        >  5:150992/1‑147 (MQ=255)
                                                                                                                             ctctTCGCGGCTCTAGCCGCCAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCACCGCATCCGGCAGCAGTGCGTCAggg                        >  8:180241/1‑147 (MQ=39)
                                                                                                                                  ggcggcTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAGGGTAGtt                   <  6:176977/147‑1 (MQ=255)
                                                                                                                                      gcTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAGGGTAGTTCTGGaa             <  2:80610/149‑1 (MQ=255)
                                                                                                                                         ctagCCGCCAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAGGGTAGTTCTGGAAGca          >  7:87198/3‑149 (MQ=255)
                                                                                                                                          gagCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGgca                                      >  2:235782/1‑120 (MQ=255)
                                                                                                                                          gagCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGgca                                      <  1:235782/120‑1 (MQ=255)
                                                                                                                                                 gAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAGGGTAGTTCTGGAAGCACACCGGgc  <  6:150992/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
AGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTG‑CAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAGGGTAGTTCTGGAAGCACACCGGGC  >  NC_002947/2951204‑2951497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑