Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,971,392 A→C 5.9% E577D (GAA→GAC) ‡ PP_2599 → chemotaxis sensory transducer family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,971,3920AC 5.9% 218.1 / 4.3 68E577D (GAA→GAC) ‡PP_2599chemotaxis sensory transducer family protein
Reads supporting (aligned to +/- strand):  ref base A (31/33);  new base C (2/2);  total (33/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

TGCAGGACGACTTCGCTTTCGTCGCCCAGGCCAACATCGGCGGCGAGTTCATGCAGGACACCGATATCCAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTGCGTGCCCTGGGGCACCCGCACGCCGCCGGTGGTGAAGGACGACCCGCGTAACATCTGGGGGTTCGACATGCATCTG  >  NC_002947/2971244‑2971539
                                                                                                                                                    |                                                                                                                                                   
tGCAGGACGACTTCGCTTTCGTCGCCCAGGCCAACATCGGCGGCGAGTTCATGCAGGACACCGATATCCAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGaa                                                                                                                                                     >  2:245145/1‑149 (MQ=255)
  cAGGACGACTTCGCTTTCGTCGCCCAGGCCAACATCGGCGGCGAGTTCATGCAGGACACCGATATCCAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACg                                                                                                                                                   >  4:183612/1‑149 (MQ=255)
   aGGACGACTTCGCTTTCGTCGCCCAGGCCAACATCGGCGGCGAGTTCATGCAGGACACCGATATCCAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGc                                                                                                                                                  <  3:183612/149‑1 (MQ=255)
     gacgacTTCGCTTTCGTCGCCCAGGCCAACATCGGCGGCGAGTTCATGCAGGACACCGATATCCAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCt                                                                                                                                                >  4:104432/1‑149 (MQ=255)
      acgacTTCGCTTTCGTCGCCCAGGCCAACATCGGCGGCGAGTTCATGCAGGACACCGATATCCAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCtt                                                                                                                                               <  5:84557/149‑1 (MQ=255)
           ttCGCTTTCGTCGCCCAGGCCAACATCGGCGGCGAGTTCATGCAGGACACCGATATCCAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCgg                                                                                                                                          >  1:81146/1‑149 (MQ=255)
             cGCTTTCGTCGCCCAGGCCAACATCGGCGGCGAGTTCATGCAGGACACCGATATCCAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTc                                                                                                                                            <  6:99416/145‑1 (MQ=255)
             cGCTTTCGTCGCCCAGGCCAACATCGGCGGCGAGTTCATGCAGGACACCGATATCCAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTc                                                                                                                                            >  5:99416/1‑145 (MQ=255)
               cTTTCGTCGCCCAGGCCAACATCGGCGGCGAGTTCATGCAGGACACCGATATCCAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTg                                                                                                                                      <  2:170860/149‑1 (MQ=255)
                         ccAGGCCAACATCGGCGGCGAGTTCATGCAGGACACCGATATCCAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACAAGGCGTTCCGCCTCGTcac                                                                                                                                          <  3:63547/135‑3 (MQ=255)
                         ccAGGCCAACATCGGCGGCGAGTTCATGCAGGACACCGATATCCAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACAAGGCGTTCCGCCTCGTcac                                                                                                                                          >  4:63547/1‑133 (MQ=255)
                                aaCATCGGCGGCGAGTTCATGCAGGACACCGATATCCAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTAcc                                                                                                                     >  4:179404/1‑149 (MQ=255)
                                        cggcgAGTTCATGCAGGACACCGATATCCAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGAc                                                                                                                  <  6:19556/144‑1 (MQ=255)
                                        cggcgAGTTCATGCAGGACACCGATATCCAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGAc                                                                                                                  >  5:19556/1‑144 (MQ=255)
                                        cggcgAGTTCATGCAGGACACCGATATCCAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAgagg                                                                                                             <  3:104432/149‑1 (MQ=255)
                                         ggcgAGTTCATGCAGGACACCGATATCCAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAgagga                                                                                                            <  7:1663/149‑1 (MQ=255)
                                              gTTCATGCAGGACACCGATATCCAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTAcc                                                                                                                     >  4:272484/1‑135 (MQ=255)
                                              gTTCATGCAGGACACCGATATCCAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTAcc                                                                                                                     <  3:272484/135‑1 (MQ=255)
                                                  aTGCAGGACACCGATATCCAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGc                                                                                                    <  3:179404/148‑1 (MQ=255)
                                                  aTGCAGGACACCGATATCCAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCt                                                                                                   >  6:133960/1‑149 (MQ=255)
                                                   tGCAGGACACCGATATCCAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTg                                                                                                  <  8:221183/149‑1 (MQ=255)
                                                   tGCAGGACACCGATATCCAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTg                                                                                                  >  4:61067/1‑149 (MQ=255)
                                                      aGGACACCGATATCCAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCACCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGcc                                                                                               <  8:291550/149‑1 (MQ=255)
                                                             cGATATCCAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGcc                                                                                        >  8:175884/1‑149 (MQ=255)
                                                             cGATATCCAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGcc                                                                                        >  1:146991/1‑149 (MQ=255)
                                                               atatCCAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGcccc                                                                                      <  8:203934/149‑1 (MQ=255)
                                                               atatCCAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACAAGGCGTTCCGCCTCGTcacg                                                                                                                                         <  3:277937/98‑4 (MQ=255)
                                                               atatCCAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACAAGGCGTTCCGCCTCGTcacg                                                                                                                                         >  4:277937/1‑95 (MQ=255)
                                                                    cgtcgcTTGCCGATCACCTGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAg                                                                                                                                <  1:122744/99‑1 (MQ=38)
                                                                    cgtcgcTTGCCGATCACCTGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAg                                                                                                                                >  2:122744/4‑102 (MQ=38)
                                                                    cAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCa                                                                                 >  4:52724/1‑149 (MQ=255)
                                                                     gtcgcTTGCCGATCACCTGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCAc                                                                                >  2:186122/3‑149 (MQ=255)
                                                                       cgcTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTg                                                                              <  7:175884/149‑1 (MQ=255)
                                                                            gCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTGCGTg                                                                          <  3:52724/148‑1 (MQ=255)
                                                                               ggTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTGCGTGCCCt                                                                      >  3:2034/1‑149 (MQ=255)
                                                                               ggTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTGCGTGCCCt                                                                      >  8:266289/1‑149 (MQ=255)
                                                                                 tGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTGCGTg                                                                          >  2:117136/1‑143 (MQ=255)
                                                                                 tGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTGCGTg                                                                          <  1:117136/143‑1 (MQ=255)
                                                                                 tGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTGCGTGCCCTgg                                                                    <  2:81146/149‑1 (MQ=255)
                                                                                  gATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTGCGTGCCCTggg                                                                   >  4:269726/1‑149 (MQ=255)
                                                                                  gATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTGCGTGCCCTggg                                                                   >  5:291309/1‑149 (MQ=255)
                                                                                   acctgCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTGCGTGCCCTggg                                                                   <  1:186122/144‑1 (MQ=255)
                                                                                   aTCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTGCGTGCCCTggg                                                                   <  4:2034/148‑1 (MQ=255)
                                                                                     cGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTGCGTGCCCTGGGGCa                                                                >  8:160340/1‑149 (MQ=255)
                                                                                      ggCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGcg                                                                                                   >  5:308515/1‑112 (MQ=255)
                                                                                      ggCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCt                                                                                                   <  6:308515/113‑1 (MQ=255)
                                                                                      ggCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTGCGTGCCCTgg                                                                    <  5:240526/144‑1 (MQ=255)
                                                                                      ggCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGAAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTGCGTGCCCTgg                                                                    >  6:240526/1‑144 (MQ=255)
                                                                                       gCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTGCGTGCCCTGGGGCAcc                                                              <  5:244128/149‑1 (MQ=255)
                                                                                          aGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTGCGTGCCCTGGGGCACCCGc                                                           <  7:160340/149‑1 (MQ=255)
                                                                                              aCGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTGCGTGCCCTGGGGCACCCGCAcgc                                                       <  7:266289/149‑1 (MQ=255)
                                                                                                 ctggctgCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTGCGTGCCCTGGGGCACCCGCAcgccgc                                                    <  5:133960/149‑1 (MQ=255)
                                                                                                     ctgCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGccc                                                                                       >  6:190331/1‑110 (MQ=255)
                                                                                                     ctgCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGccc                                                                                       <  5:190331/110‑1 (MQ=255)
                                                                                                            aTTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGa                                                                                                                   <  2:286778/75‑1 (MQ=255)
                                                                                                            aTTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGa                                                                                                                   >  1:286778/1‑75 (MQ=255)
                                                                                                            aTTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTGCGTGCCCTGGGGCACCCGCACGCCGCCGgtggtg                                            >  5:57119/1‑146 (MQ=255)
                                                                                                            aTTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTGCGTGCCCTGGGGCACCCGCACGCCGCCGGTGGTGaa                                          <  6:57119/148‑1 (MQ=255)
                                                                                                               tCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTGCGTGCCCTGGGGCACCCGCACGCCGCCGGTGGTGAAGga                                       <  4:24400/148‑1 (MQ=255)
                                                                                                                  aTAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTGCGTGCCCTGGGGCACCCGCACGCCGCCGGTGGTGAAGgacgac                                   >  1:267877/1‑149 (MQ=255)
                                                                                                                     aCCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTGCGTGCCCTGGGGCACCCGCACGCCGCCGGTGGTGAAg                                         <  8:125397/140‑1 (MQ=255)
                                                                                                                     aCCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTGCGTGCCCTGGGGCACCCGCACGCCGCCGGGGGAGAAg                                         >  7:125397/1‑140 (MQ=255)
                                                                                                                            gggCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTGCGTGCCCTGGGGCACCCGCACGCCGCCGGTGGTGAAGGACGACCCGCGTAAc                          >  2:38915/1‑148 (MQ=255)
                                                                                                                                     ccGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTGCGTGCCCTGGGGCACCCGCACGCCGCCGGTGGTGAAGGACGACCCGCGTAACATCTgg                    <  3:61067/145‑1 (MQ=255)
                                                                                                                                     ccGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGAACTGCGTGCCCTGGGGCACCCCCACGCCGCCGGTGGTGAAGGACGACCCGCGTAACATCTggggct                >  4:36932/1‑147 (MQ=255)
                                                                                                                                       gTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTGCGTGCCCTGGGGCACCCGCACGCCGCCGGTGGTGAAGGACGACCCGCGTAACATCTGGGGGTTc              >  3:250604/1‑149 (MQ=255)
                                                                                                                                         gACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGa                                                                                    <  7:217252/77‑1 (MQ=255)
                                                                                                                                         gACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGa                                                                                    >  8:217252/1‑77 (MQ=255)
                                                                                                                                          aCGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTGCGTGCCCTGGGGCACCCGCACGCCGCCGGTGGTGAAGGACGACCCGCGTAACATCTGGGGGTTCGAc           <  8:171949/149‑1 (MQ=255)
                                                                                                                                                   aaCGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTGCGTGCCCTGGGGCACCCGCACGCCGCCGGTGGTGAAGGACGACCCGCGTAACATCTGGGGGTTCGACATGCATCTg  <  1:245145/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGCAGGACGACTTCGCTTTCGTCGCCCAGGCCAACATCGGCGGCGAGTTCATGCAGGACACCGATATCCAGCGCTTGCAGGTGATCGGCAAGCGACGCTGGCTGCGCCATTTCGATAACCGCCTGGGCATCTCCCGTGACGAGGCGGAACGCCTTGTCGGCGTGCCGCAGCCTGAACTACCGACAGAGGAGCCATTGCTGGCCGACCGCCCCGAGCACTGCGTGCCCTGGGGCACCCGCACGCCGCCGGTGGTGAAGGACGACCCGCGTAACATCTGGGGGTTCGACATGCATCTG  >  NC_002947/2971244‑2971539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑