Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,006,479 C→A 10.2% D1020E (GAC→GAA)  PP_2627 → membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,006,4790CA10.2% 146.8 / 7.0 49D1020E (GAC→GAA) PP_2627membrane protein
Reads supporting (aligned to +/- strand):  ref base C (25/19);  new base A (3/2);  total (28/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

TTTTTTCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCAGCATCGAGCCCTTG  >  NC_002947/3006335‑3006626
                                                                                                                                                |                                                                                                                                                    
ttttttCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAAcc                                                                                                                                                  <  5:73460/149‑1 (MQ=255)
     tCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAg                                                                                                                                             >  3:187700/1‑149 (MQ=255)
        aGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTGTCCTTCAGGAAAAcc                                                                                                                                                  >  3:294750/1‑141 (MQ=255)
        aGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTGTCCTTCAGGAAAAcc                                                                                                                                                  <  4:294750/141‑1 (MQ=255)
                  ggCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCt                                                                                                                                                 >  7:143462/1‑132 (MQ=255)
                  ggCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCt                                                                                                                                                 <  8:143462/132‑1 (MQ=255)
                  ggCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCg                                                                                                                                >  3:309768/1‑149 (MQ=255)
                     aGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTc                                                                                                                             <  5:211581/149‑1 (MQ=255)
                              ccccTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACggg                                                                                                                     <  4:187700/148‑1 (MQ=255)
                                 cTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AAt                                                                                                                >  2:120563/1‑149 (MQ=255)
                                 cTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AAt                                                                                                                >  7:238551/1‑149 (MQ=255)
                                           aaGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCg                                                                                                      <  8:238551/149‑1 (MQ=255)
                                                  ccGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGAc                                                                                               >  1:274078/1‑149 (MQ=255)
                                                        cttcttTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATc                                                                                         <  7:61171/149‑1 (MQ=255)
                                                        cttcttTGGATGACTTCGAGGCGTTATTCGGGCAGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATc                                                                                         >  2:64981/1‑149 (MQ=255)
                                                         ttcttTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATc                                                                                         >  2:170724/1‑148 (MQ=255)
                                                              tGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGagc                                                                                     <  4:309768/147‑1 (MQ=255)
                                                                gATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGAGCAGCt                                                                                 <  1:120563/149‑1 (MQ=255)
                                                                 aTGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGAGCAGCTc                                                                                <  4:301822/149‑1 (MQ=255)
                                                                          aGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCt                                                                                                            >  2:275662/1‑112 (MQ=255)
                                                                          aGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCt                                                                                                            <  1:275662/112‑1 (MQ=255)
                                                                            gCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCg                                                                       >  5:75603/1‑147 (MQ=255)
                                                                               ttcttcGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAgcg                                                                  >  2:204550/1‑149 (MQ=255)
                                                                                     gggCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATcc                                                             <  2:274078/148‑1 (MQ=255)
                                                                                      ggCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCcgc                                                           >  4:110104/1‑149 (MQ=255)
                                                                                        ccGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCcgcg                                                          >  2:246531/1‑148 (MQ=255)
                                                                                                 cgcCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACcttctt                                                <  7:8133/149‑1 (MQ=255)
                                                                                                          cagTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCg                                       >  6:137448/1‑149 (MQ=255)
                                                                                                            gTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTg                                     >  1:291224/1‑149 (MQ=255)
                                                                                                             tttAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTgg                                    <  6:75603/149‑1 (MQ=255)
                                                                                                                    aTCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTa                             <  3:110104/149‑1 (MQ=255)
                                                                                                                     tCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTaa                            <  5:137448/149‑1 (MQ=255)
                                                                                                                           tctcAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGc                      >  6:90085/1‑149 (MQ=255)
                                                                                                                             tcAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAg                    >  8:1624/1‑149 (MQ=255)
                                                                                                                             tcAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAg                    >  5:286064/1‑149 (MQ=255)
                                                                                                                             tcAAGGTTGTCCTTCAGGAAAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAg                    >  3:229711/1‑149 (MQ=255)
                                                                                                                              cAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGt                   >  5:299574/1‑149 (MQ=255)
                                                                                                                               aaGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCa                                  <  1:129843/133‑1 (MQ=255)
                                                                                                                               aaGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCa                                  >  2:129843/1‑133 (MQ=255)
                                                                                                                               aaGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCa                     <  1:204550/146‑1 (MQ=255)
                                                                                                                               aaGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGtt                  >  3:143480/1‑149 (MQ=255)
                                                                                                                                 ggTTGTCCTTCAGGAAAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCa                                  <  7:36267/131‑1 (MQ=255)
                                                                                                                                 ggTTGTCCTTCAGGAAAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCa                                  >  8:36267/1‑131 (MQ=255)
                                                                                                                                       ccTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCAGCATc           <  4:143480/148‑1 (MQ=255)
                                                                                                                                         tGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCAGCATCGAg        >  2:168992/1‑149 (MQ=255)
                                                                                                                                              gACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCAGCATCGAGCCCtt   >  5:267710/1‑149 (MQ=255)
                                                                                                                                              gACAACCTTGAGGCCTTGCAGTCCGCTCAGCAAGCGC‑AATCCCTGATACGTGCCGACGTGATCGAGCAGCACGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCAGCATCTAGCACtt   >  3:219257/1‑149 (MQ=255)
                                                                                                                                               aCAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAAATCCCTGATTCTTGCCGACTTGATCGACCAGATCGAGATGGCGGAGCGCATCCGCGAAACCTAGTACGATCAGCGTGGCAAGCTAAGTGTGCa                     >  1:53415/1‑131 (MQ=255)
                                                                                                                                               aCAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCAGCATCGAGCCCTTg  <  1:170724/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TTTTTTCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGC‑AATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCAGCATCGAGCCCTTG  >  NC_002947/3006335‑3006626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑