Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,034,248 G→A 8.3% A314V (GCC→GTC)  PP_2647 ← MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,034,2480GA 8.3% 171.9 / 4.2 61A314V (GCC→GTC) PP_2647MFS transporter
Reads supporting (aligned to +/- strand):  ref base G (25/30);  new base A (2/3);  total (27/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01

GAACGAGCCCACGGCAATGATCACCTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGGCAGCAAAGCCGGCGAAGATGTTGCCCATGAAGCCGGCCACGCCATA  >  NC_002947/3034100‑3034393
                                                                                                                                                    |                                                                                                                                                 
gaaCGAGCCCACGGCAATGATCACCTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGAGCAAGCCGGGAACGCACCGAAGGCAAAGACCCATAGTGCGATCAGCATgg                                                                                                                                                   >  7:158231/1‑149 (MQ=255)
 aaCGAGCCCACGGCAATGATCACCTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATggc                                                                                                                                                  >  6:311742/1‑149 (MQ=255)
  aCGAGCCCACGGCAATGATCACCTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATggcg                                                                                                                                                 >  4:10673/1‑149 (MQ=255)
     aGCCCACGGCAATGATCACCTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCa                                                                                                                                              >  4:107019/1‑149 (MQ=255)
      gCCCACGGCAATGATCACCTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGAGCCACGACAAACATACAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCAc                                                                                                                                             >  3:202491/1‑149 (MQ=255)
          aCGGCAATGATCACCTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGt                                                                                                                                         >  8:102292/1‑149 (MQ=255)
               aaTGATCACCTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGc                                                                                                                                    <  5:44918/149‑1 (MQ=255)
                   aTCACCTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGACGGGTGCCGCCATGctga                                                                                                                                 <  7:252138/148‑4 (MQ=255)
                    tCACCTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTg                                                                                                                               >  8:242976/1‑149 (MQ=255)
                    tCACCTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTg                                                                                                                               >  3:140994/1‑149 (MQ=255)
                    tCACCTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTg                                                                                                                               <  3:107019/149‑1 (MQ=255)
                    tCACCTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTg                                                                                                                               <  2:179400/149‑1 (MQ=255)
                    tCACCTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTg                                                                                                                               <  6:31971/149‑1 (MQ=255)
                    tCACCTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTg                                                                                                                               <  8:139649/149‑1 (MQ=255)
                      aCCTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGc                                                                                                                             >  1:76822/1‑149 (MQ=255)
                       ccTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCg                                                                                                                            <  2:49621/149‑1 (MQ=255)
                        cTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGACGGGTGCCGCCATGct                                                                                                                                   >  6:172334/1‑140 (MQ=255)
                        cTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGACGGGTGCCGCCATGct                                                                                                                                   <  5:172334/141‑2 (MQ=255)
                          ggAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTgg                                                                                                                              >  2:193904/1‑144 (MQ=255)
                          ggAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTgg                                                                                                                              <  1:193904/144‑1 (MQ=255)
                           gAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGaa                                                                                                                          <  3:10673/147‑1 (MQ=255)
                           gAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGt                                                                                                                        >  4:52928/1‑149 (MQ=255)
                               aTGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCgg                                                                                                                    >  2:213036/1‑149 (MQ=255)
                                tGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGACGGGTGCCGCCATGct                                                                                                                                   >  3:305574/1‑132 (MQ=255)
                                tGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGACGGGTGCCGCCATGct                                                                                                                                   <  4:305574/133‑2 (MQ=255)
                                 ggCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCggg                                                                                                                   >  5:288364/1‑148 (MQ=255)
                                          cAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCgg                                                                                                         <  8:7809/149‑1 (MQ=255)
                                             cGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGc                                                                                                      <  1:171535/149‑1 (MQ=255)
                                                 aTGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGAc                                                                                                  <  5:311742/149‑1 (MQ=255)
                                                    ccACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGc                                                                                               >  8:123088/1‑149 (MQ=255)
                                                     cACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATgg                                                                                                                                                   >  4:178548/1‑96 (MQ=255)
                                                     cACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATgg                                                                                                                                                   <  3:178548/96‑1 (MQ=255)
                                                        gTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGAt                                                                                            >  6:223938/1‑148 (MQ=255)
                                                            gACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTg                                                                                                                               <  2:202117/109‑1 (MQ=255)
                                                            gACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTg                                                                                                                               >  1:202117/1‑109 (MQ=255)
                                                              cATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAgc                                                                                     >  3:98915/1‑149 (MQ=255)
                                                               aTCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAgcg                                                                                    >  2:96046/1‑149 (MQ=255)
                                                                    tGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAgcgc                                                                                   <  7:102292/145‑1 (MQ=255)
                                                                       gcgcCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGaa                                                                                                                          >  2:323166/1‑103 (MQ=255)
                                                                       gcgcCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGaa                                                                                                                          <  1:323166/103‑1 (MQ=255)
                                                                       gcgcCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGc                                                                            <  4:98915/149‑1 (MQ=255)
                                                                       gcgcCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGc                                                                            <  4:140994/149‑1 (MQ=255)
                                                                         gcCACGACAAACATCCAGATATTGGCGCTAGCCGGGAACGCTGCGAAGGCAAAGCCCCATAGTGCTATCAGCATGTCGGCAACCGTCATGCCGGTGGCTAAGTGCTGGAACAGTGCTGTGCTGAGTGCTATCATGTGCGCGACCAGCAt                                                                          <  8:158231/149‑1 (MQ=255)
                                                                               aCAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAggg                                                                     >  5:66656/1‑148 (MQ=255)
                                                                               aCAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGt                                                                    <  3:52928/149‑1 (MQ=255)
                                                                                 aaaCATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAgg                                                                      <  5:90500/145‑1 (MQ=255)
                                                                                 aaaCATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAgg                                                                      >  6:90500/1‑145 (MQ=255)
                                                                                  aaCATCCAGATTTTGGCGCAAGCCGGGAACGCACCGTAGGCATAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTTCGTGAACAGGGCGGTGGTGACTGGGATCATGAGCGCGACCAGCATCAGGGTATg                                                                 <  4:202491/149‑1 (MQ=255)
                                                                                       ccAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCa                                                            >  1:154288/1‑149 (MQ=255)
                                                                                        cAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCAc                                                           >  7:234880/1‑149 (MQ=255)
                                                                                                     aaGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGgca                                              <  6:288364/149‑1 (MQ=255)
                                                                                                          gggAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGGCAGCaaa                                         <  3:45226/149‑1 (MQ=255)
                                                                                                                  aacgcaGGAAAAGCCCCATAGTGAGATCAGCATGGCGGCACCCGTCATGCCGGTGCCGAAGTGCGGGAACAGGGCGGTGCTCACTGCGATCATGAGCGCGCCCCGCATCAGGGTATGCCGCACCCTGCGGTTGGCAGAAAAGCCGGCg                                  >  2:153652/6‑148 (MQ=255)
                                                                                                                    cGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGGCAGCAAAGCCGGCg                                  >  6:107715/1‑146 (MQ=255)
                                                                                                                      aaGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGGCAGCAAAGCCGGCGAAGAt                             <  2:76822/149‑1 (MQ=255)
                                                                                                                           aaaGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGGCAGCAAAGCCGGCGAAGATGTTGc                        <  1:213036/149‑1 (MQ=255)
                                                                                                                                cccATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGGCAGCAAAGCCGGCGAAGAAGTTGCCCAt                    >  3:82915/1‑148 (MQ=255)
                                                                                                                                 ccATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGt                                                   <  6:43320/116‑1 (MQ=255)
                                                                                                                                 ccATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGt                                                   >  5:43320/1‑116 (MQ=255)
                                                                                                                                 ccATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGGCAGCAAAGCCGGCGAAGATGTTGCCCATGa                  <  7:242976/149‑1 (MQ=255)
                                                                                                                                  cATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGGCAGCAAAGCCGGCGAAGATGTTGCCCATGaa                 <  7:139974/149‑1 (MQ=255)
                                                                                                                                      gTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGTATCAGGGTATGCCGCACGCTGCGGTTGGCAGCAAAGCCGGCGAAGATGTTGCCCATGAAGcc              <  1:96046/148‑1 (MQ=255)
                                                                                                                                         cGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGGCAGCAAAGCCGGCGAAGATGTTGCCCATGAAGCCGGcc          <  5:107715/149‑1 (MQ=255)
                                                                                                                                         cGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGGCAGCAAAGCCGGCGAAGATGTTGCCCATGAAGCCGGc           <  6:66656/148‑1 (MQ=255)
                                                                                                                                         cGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGGTGGCAGCAAAGCCGGCGAAGATGTTGCCCATGAAGCCGGc           <  8:234880/148‑1 (MQ=255)
                                                                                                                                                 aTGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGGCAGCAAAGCCGGCGAAGATGTTGCCCATGAAGCCGGCCACGCCATa  >  1:263621/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GAACGAGCCCACGGCAATGATCACCTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGGCAGCAAAGCCGGCGAAGATGTTGCCCATGAAGCCGGCCACGCCATA  >  NC_002947/3034100‑3034393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑