Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,034,258 T→C 8.0% T311A (ACG→GCG)  PP_2647 ← MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,034,2580TC 8.0% 175.2 / 5.0 62T311A (ACG→GCG) PP_2647MFS transporter
Reads supporting (aligned to +/- strand):  ref base T (23/34);  new base C (2/3);  total (25/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01

ACGGCAATGATCACCTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGGCAGCAAAGCCGGCGAAGATGTTGCCCATGAAGCCGGCCACGCCATACAGCAGCAACAAT  >  NC_002947/3034110‑3034406
                                                                                                                                                    |                                                                                                                                                    
aCGGCAATGATCACCTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGt                                                                                                                                                      >  8:102292/1‑149 (MQ=255)
     aaTGATCACCTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGc                                                                                                                                                 <  5:44918/149‑1 (MQ=255)
         aTCACCTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGACGGGTGCCGCCATGctga                                                                                                                                              <  7:252138/148‑4 (MQ=255)
          tCACCTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTg                                                                                                                                            >  8:242976/1‑149 (MQ=255)
          tCACCTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTg                                                                                                                                            <  3:107019/149‑1 (MQ=255)
          tCACCTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTg                                                                                                                                            >  3:140994/1‑149 (MQ=255)
          tCACCTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTg                                                                                                                                            <  2:179400/149‑1 (MQ=255)
          tCACCTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTg                                                                                                                                            <  6:31971/149‑1 (MQ=255)
          tCACCTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTg                                                                                                                                            <  8:139649/149‑1 (MQ=255)
            aCCTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGc                                                                                                                                          >  1:76822/1‑149 (MQ=255)
             ccTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCg                                                                                                                                         <  2:49621/149‑1 (MQ=255)
              cTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGACGGGTGCCGCCATGct                                                                                                                                                >  6:172334/1‑140 (MQ=255)
              cTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGACGGGTGCCGCCATGct                                                                                                                                                <  5:172334/141‑2 (MQ=255)
                ggAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTgg                                                                                                                                           >  2:193904/1‑144 (MQ=255)
                ggAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTgg                                                                                                                                           <  1:193904/144‑1 (MQ=255)
                 gAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGaa                                                                                                                                       <  3:10673/147‑1 (MQ=255)
                 gAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGt                                                                                                                                     >  4:52928/1‑149 (MQ=255)
                     aTGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCgg                                                                                                                                 >  2:213036/1‑149 (MQ=255)
                      tGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGACGGGTGCCGCCATGct                                                                                                                                                <  4:305574/133‑2 (MQ=255)
                      tGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGACGGGTGCCGCCATGct                                                                                                                                                >  3:305574/1‑132 (MQ=255)
                       ggCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCggg                                                                                                                                >  5:288364/1‑148 (MQ=255)
                                cAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCgg                                                                                                                      <  8:7809/149‑1 (MQ=255)
                                   cGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGc                                                                                                                   <  1:171535/149‑1 (MQ=255)
                                       aTGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGAc                                                                                                               <  5:311742/149‑1 (MQ=255)
                                          ccACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGc                                                                                                            >  8:123088/1‑149 (MQ=255)
                                              gTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGAt                                                                                                         >  6:223938/1‑148 (MQ=255)
                                                  gACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTg                                                                                                                                            <  2:202117/109‑1 (MQ=255)
                                                  gACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTg                                                                                                                                            >  1:202117/1‑109 (MQ=255)
                                                    cATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAgc                                                                                                  >  3:98915/1‑149 (MQ=255)
                                                     aTCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAgcg                                                                                                 >  2:96046/1‑149 (MQ=255)
                                                          tGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAgcgc                                                                                                <  7:102292/145‑1 (MQ=255)
                                                             gcgcCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGaa                                                                                                                                       >  2:323166/1‑103 (MQ=255)
                                                             gcgcCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGaa                                                                                                                                       <  1:323166/103‑1 (MQ=255)
                                                             gcgcCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGc                                                                                         <  4:140994/149‑1 (MQ=255)
                                                             gcgcCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGc                                                                                         <  4:98915/149‑1 (MQ=255)
                                                               gcCACGACAAACATCCAGATATTGGCGCTAGCCGGGAACGCTGCGAAGGCAAAGCCCCATAGTGCTATCAGCATGTCGGCAACCGTCATGCCGGTGGCTAAGTGCTGGAACAGTGCTGTGCTGAGTGCTATCATGTGCGCGACCAGCAt                                                                                       <  8:158231/149‑1 (MQ=255)
                                                                     aCAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAggg                                                                                  >  5:66656/1‑148 (MQ=255)
                                                                     aCAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGt                                                                                 <  3:52928/149‑1 (MQ=255)
                                                                       aaaCATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAgg                                                                                   >  6:90500/1‑145 (MQ=255)
                                                                       aaaCATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAgg                                                                                   <  5:90500/145‑1 (MQ=255)
                                                                        aaCATCCAGATTTTGGCGCAAGCCGGGAACGCACCGTAGGCATAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTTCGTGAACAGGGCGGTGGTGACTGGGATCATGAGCGCGACCAGCATCAGGGTATg                                                                              <  4:202491/149‑1 (MQ=255)
                                                                             ccAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCa                                                                         >  1:154288/1‑149 (MQ=255)
                                                                              cAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCAc                                                                        >  7:234880/1‑149 (MQ=255)
                                                                                           aaGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGgca                                                           <  6:288364/149‑1 (MQ=255)
                                                                                                gggAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGGCAGCaaa                                                      <  3:45226/149‑1 (MQ=255)
                                                                                                        aacgcaGGAAAAGCCCCATAGTGAGATCAGCATGGCGGCACCCGTCATGCCGGTGCCGAAGTGCGGGAACAGGGCGGTGCTCACTGCGATCATGAGCGCGCCCCGCATCAGGGTATGCCGCACCCTGCGGTTGGCAGAAAAGCCGGCg                                               >  2:153652/6‑148 (MQ=255)
                                                                                                          cGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGGCAGCAAAGCCGGCg                                               >  6:107715/1‑146 (MQ=255)
                                                                                                            aaGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGGCAGCAAAGCCGGCGAAGAt                                          <  2:76822/149‑1 (MQ=255)
                                                                                                                 aaaGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGGCAGCAAAGCCGGCGAAGATGTTGc                                     <  1:213036/149‑1 (MQ=255)
                                                                                                                      cccATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGGCAGCAAAGCCGGCGAAGAAGTTGCCCAt                                 >  3:82915/1‑148 (MQ=255)
                                                                                                                       ccATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGt                                                                >  5:43320/1‑116 (MQ=255)
                                                                                                                       ccATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGt                                                                <  6:43320/116‑1 (MQ=255)
                                                                                                                       ccATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGGCAGCAAAGCCGGCGAAGATGTTGCCCATGa                               <  7:242976/149‑1 (MQ=255)
                                                                                                                        cATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGGCAGCAAAGCCGGCGAAGATGTTGCCCATGaa                              <  7:139974/149‑1 (MQ=255)
                                                                                                                            gTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGTATCAGGGTATGCCGCACGCTGCGGTTGGCAGCAAAGCCGGCGAAGATGTTGCCCATGAAGcc                           <  1:96046/148‑1 (MQ=255)
                                                                                                                               cGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGGCAGCAAAGCCGGCGAAGATGTTGCCCATGAAGCCGGcc                       <  5:107715/149‑1 (MQ=255)
                                                                                                                               cGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGGCAGCAAAGCCGGCGAAGATGTTGCCCATGAAGCCGGc                        <  6:66656/148‑1 (MQ=255)
                                                                                                                               cGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGGTGGCAGCAAAGCCGGCGAAGATGTTGCCCATGAAGCCGGc                        <  8:234880/148‑1 (MQ=255)
                                                                                                                                       aTGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGGCAGCAAAGCCGGCGAAGATGTTGCCCATGAAGCCGGCCACGCCATa               >  1:263621/1‑149 (MQ=255)
                                                                                                                                           cggcACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGGCAGCAAAGCCTGCGAAGATGTTGCCCATGAAGCCGGCCACGCCATAcagc           <  5:223938/149‑1 (MQ=255)
                                                                                                                                           cggcACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGGCAGCAAAGCCGGCGAAGATGTTGCCCATGAAGCCGGCCACGCCATAcagc           <  2:263621/149‑1 (MQ=255)
                                                                                                                                               aCCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGGCAGCAAAGCCGGCGAAGATGTTGccc                                   >  2:253105/1‑121 (MQ=255)
                                                                                                                                               aCCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGGCAGCAAAGCCGGCGAAGATGTTGccc                                   <  1:253105/121‑1 (MQ=255)
                                                                                                                                                    tCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGGCAGCAAAGCCGGCGAAGATGTTGCCCATGAAGCCGGCCACGCCATACAGCAGCAACAAt  >  2:88506/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ACGGCAATGATCACCTGGAACATGGCCACGAACAGCGGCATGCCACGTTCGACATCTTTGGGCGCCACGACAAACATCCAGATATTGGCGCAAGCCGGGAACGCACCGAAGGCAAAGCCCCATAGTGCGATCAGCATGGCGGCACCCGTCATGCCGGTGGCGAAGTGCGGGAACAGGGCGGTGCTGACTGCGATCATGAGCGCGACCAGCATCAGGGTATGCCGCACGCTGCGGTTGGCAGCAAAGCCGGCGAAGATGTTGCCCATGAAGCCGGCCACGCCATACAGCAGCAACAAT  >  NC_002947/3034110‑3034406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑