Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,062,728 A→G 9.3% V245A (GTG→GCG)  qedH‑I ← quinoprotein ethanol dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,062,7280AG 9.3% 115.2 / 3.3 43V245A (GTG→GCG) qedH‑Iquinoprotein ethanol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (17/22);  new base G (2/2);  total (19/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

CGCGCTTTGCCAAGGGGCACCGCCGCCGTGGCTCCAGGCTTCGACCTTGCCGGTTTCGGTGGTAGGGTCGTCTGGCCAGGACGGCGCCTTGACGTCACCGGTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACACTTCTTCACCGGTGTCCGGGTCGCGCGCGAACAGCTGGCCGACCACGCCGAATTCATCGCCGGAGCTGCCGTGGATCAGCAGCACCTTGCCGCTGGTCTTGTCCTTGATCAGCACTGGCGCACCGGTCAT  >  NC_002947/3062583‑3062876
                                                                                                                                                 |                                                                                                                                                    
cgcgCTTTGCCAAGGGGCACCGCCGCCGTGGCTCCAGGCTTCGACCTTGCCGGTTTCGGTGGTAGGGTCGTCTGGCCAGGACGGCGCCTTGACGTCACCGGTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACg                                                                                                                                                    >  2:186742/1‑148 (MQ=255)
  cgcTTTGCCAAGGGGCACCGCCGCCGTGGCTCCAGGCTTCGACCTTGCCGGTTTCGGTGGTAGGGTCGTCTGGCCAGGACGGCGCCTTGACGTCACCGGTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAg                                                                                                                                                 <  6:35339/149‑1 (MQ=255)
               ggCACCGCCGCCGTGGCTCCAGGCTTCGACCTTGCCGGTTTCGGTGGTAGGGTCGTCTGGCCAGGACGGCGCCTTGACGTCACCGGTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTTCGTGGAGGGCCACAt                                                                                                                                         <  1:186055/144‑1 (MQ=255)
               ggCACCGCCGCCGTGGCTCCAGGCTTCGACCTTGCCGGTTTCGGTGGTAGGGTCGTCTGGCCAGGACGGCGCCTTGACGTCACCGGTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTTCGTGGAGGGCCACAt                                                                                                                                         >  2:186055/1‑144 (MQ=255)
                 caacgccgccgTGGCTCCAGGCTTCGACCTTGCCGGTTTCGGTGGTAGGGTCGTCTGGCCAGGACGGCGCCTTGACGTCACCGGTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACAct                                                                                                                                  >  5:327604/4‑149 (MQ=255)
                    cgccgccgTGGCTCCAGGCTTCGACCTTGCCGGTTTCGGTGGTAGGGTCGTCTGGCCAGGACGGCGCCTTGACGTCACCGGTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACActtct                                                                                                                               <  3:129467/149‑1 (MQ=255)
                    cgccgccgTGGCTCCAGGCTTCGACCTTGCCGGTTTCGGTGGTAGGGTCGTCTGGCCAGGACGGCGCCTTGACGTCACCGGTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACActtct                                                                                                                               >  3:193053/1‑149 (MQ=255)
                     gccgccgTGGCTCCAGGCTTCGACCTTGCCGGTTTCGGTGGTAGGGTCGTCTGGCCAGGACGGCGCCTTGACGTCACCGGTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACActtctt                                                                                                                              <  7:166575/149‑1 (MQ=255)
                     gccgccgTGGCTCCAGGCTTCGACCTTGCCGGTTTCGGTGGTAGGGTCGTCTGGCCAGGACGGCGCCTTGACGTCACCGGTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACActtctt                                                                                                                              <  1:186742/149‑1 (MQ=255)
                          cgTGGCTCCAGGCTTCGACCTTGCCGGTTTCGGTGGTAGGGTCGTCTGGCCAGGACGGCGCCTTGACGTCACCGGTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACACTTCTTCACCg                                                                                                                         <  3:215436/149‑1 (MQ=255)
                              gCTCCAGGCTTCGACCTTGCCGGTTTCGGTGGTAGGGTCGTCTGGCCAGGACGGCGCCTTGACGTCACCGGTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCGCGAAGGGCCGCATCCACActt                                                                                                                                 <  1:314298/137‑1 (MQ=255)
                              gCTCCAGGCTTCGACCTTGCCGGTTTCGGTGGTAGGGTCGTCTGGCCAGGACGGCGCCTTGACGTCACCGGTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCGCGAAGGGCCGCATCCACActt                                                                                                                                 >  2:314298/1‑137 (MQ=255)
                                 ccAGGCTTCGACCTTGCCGGTTTCGGTGGTAGGGTCGTCTGGCCAGGACGGCGCCTTGACGTCACCGGTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACACTTCTTCACCGGTGTCCg                                                                                                                  >  3:101484/1‑149 (MQ=255)
                                  cAGGCTTCGACCTTGCCGGTTTCGGTGGTAGGGTCGTCTGGCCAGGACGGCGCCTTGACGTCACCGGTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACACTTCTTCACCGGTGTCCgg                                                                                                                 <  7:18367/149‑1 (MQ=255)
                                       ttCGACCTTGCCGGTTTCGGTGGTAGGGTCGTCTGGCCAGGACGGCGCCTTGACGTCACCGGTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACACTTCTTCACCGGTGTCCGGGTcgc                                                                                                            <  4:25841/149‑1 (MQ=255)
                                            ccTTGCCGGTTTCGGTGGTAGGGTCGTCTGGCCAGGACGGCGCCTTGACGTCACCGGTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACACTTCTTCACCGGTGTCCGGGTcgcgcgc                                                                                                        >  7:122381/1‑148 (MQ=255)
                                                         ggtggtAGGGTCGTCTGGCCAGGACGGCGCCTTGACGTCACCGGTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACACTTCTTCACCGGTGTCCGGGTCGCGCGCGAACAGCTGGCCGa                                                                                          >  4:215792/1‑149 (MQ=255)
                                                          gtggtAGGGTCGTCTGGCCAGGACGGCGCCTTGACGTCACCGGTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACACTTCTTCACCGGTGTCCGGGTCGCGCGCGAACAGCTGGCCGAc                                                                                         <  8:122381/149‑1 (MQ=255)
                                                                     gtcTGGCCAGGACGGCGCCTTGACGTCACCGGTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACActtct                                                                                                                               <  6:329694/100‑1 (MQ=255)
                                                                     gtcTGGCCAGGACGGCGCCTTGACGTCACCGGTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACAcatct                                                                                                                               >  5:329694/1‑100 (MQ=255)
                                                                          gCCAGGACGGCGCCTTGACGTCACCGGTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACACTTCTTCACCGGTGTCCGGGTCGCGCGCGAACAGCTGGCCGACCACGCCGAATTCATCg                                                                         >  2:6189/1‑149 (MQ=255)
                                                                                     gcCTTGACGTCACCGGTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACACTTCTTCACCGGTGTCCGGGTCGCGCGCGAACAGCTGGCCGAcc                                                                                        <  3:312940/123‑1 (MQ=255)
                                                                                     gcCTTGACGTCACCGGTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACACTTCTTCACCGGTGTCCGGGTCGCGCGCGAACAGCTGGCCGAcc                                                                                        >  4:312940/1‑123 (MQ=255)
                                                                                       cTTGACGTCACCGGTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACACTTCTTCACCGGTGTCCGGGTCGCGCGCGAACAGCTGGCCGACCACGCCGAATTCATCGCCGGAGCTGCCGt                                                            <  8:270539/149‑1 (MQ=255)
                                                                                           aCGTCACCGGTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACACTTCTTCACCGGTGTCCGGGTCGCGCGCGAACAGCTGGCCGACCACGCCGAATTCATCGCCGGAGCTGCCGTGGa                                                         <  5:191442/148‑1 (MQ=255)
                                                                                               cACCGGTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACActt                                                                                                                                 <  6:268816/72‑1 (MQ=255)
                                                                                               cACCGGTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACActt                                                                                                                                 >  5:268816/1‑72 (MQ=255)
                                                                                                   ggTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACACTTCTTCACCGGTGTCCGGGTCGCGCGCGAACAGCTGGCCGACCACg                                                                                     <  3:21456/112‑1 (MQ=255)
                                                                                                   ggTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACACTTCTTCACCGGTGTCCGGGTCGCGCGCGAACAGCTGGCCGACCACg                                                                                     >  4:21456/1‑112 (MQ=255)
                                                                                                                tCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACACTTCTTCACCGGTGTCCGGGTCGCGCGCGAACAGCTGGCCGACCACGCCGAATTCATCGCCGGAGCTGCCGTGGATCAGCAGCACCTTGCCGCTgg                                    >  8:48935/1‑148 (MQ=255)
                                                                                                                tCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACACTTCTTCACCGGTGTCCGGGTCGCGCGCGAACAGCTGGCCGACCACGCCGAATTCATCGCCGGAGCTGCCGTGCATCAGCAGCACCTTGCCGCTgg                                    <  7:48935/148‑1 (MQ=255)
                                                                                                                  cTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACACTTCTTCACCGGTGTCCGGGTCGCGCGCGAACAGCTGGCCGACCACGCCGAATTCATCGCCGGAGCTGCCGTGGATCAGCAGCACCTTGCCGCTGGTc                                  <  2:152409/148‑1 (MQ=255)
                                                                                                                  cTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACACTTCTTCACCGGTGTCCGGGTCGCGCGCGAACAGCTGGCCGACCACGCCGAATTCATCGCCGGAGCTGCCGTGGATCAGCAGCACCTTGCCGCTGGTCt                                 >  3:39088/1‑149 (MQ=255)
                                                                                                                  cTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACACTTCTTCACCGGTGTCCGGGTCGCGCGCGAACAGCTGGCCGACCACGCCGAATTCATCGCCGGAGCTGCCGTGGATCAGCAGCACCTTGCCGCTGGTCt                                 >  1:109597/1‑149 (MQ=255)
                                                                                                                     gCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACACTTCTTCACCGGTGTCCGGGTCGCGCGCGAACAGCTGGCCGACCACGCCGAATTCATCGCCGGAGCTGCCGTGGATCAGCAGCACCTTGCCGCTGGTCTTg                               <  4:39088/148‑1 (MQ=255)
                                                                                                                     gCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACACTTCTTCACCGGTGTCCGGGTCGCGCGCGAACAGCTGGCCGACCACGCCGAATTCATCGCCGGAGCTGCCGTGGATCAGCAGCACCTTGCCGCTGGTCTTg                               <  2:109597/148‑1 (MQ=255)
                                                                                                                         ttCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACACTTCTTCACCGGTGTCCGGGTCGCGCGCGAACAGCTGGCCGACCACGCCGAATTCATCGCCGGAGCTGCCGTGGATCAGCAGCACCTTGCCGCTGGTCTTGTCCtt                          <  1:6189/149‑1 (MQ=255)
                                                                                                                           cAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACACTTCTTCACCGGTGTCCGGGTCGCGCGCGAACAGCTGGCCGACCACGCCGAATTCATCGCCGGAGCTGCCGTGGATCAGCAGCACCTTGCCGCTGGTCTTGTCCTTg                         <  5:275924/148‑1 (MQ=255)
                                                                                                                              gcggcCCATGTGGCCCTCCACGAAGGGCCGCATCCACACTTCTTCACCGGTGTCCGGGTcgcgcgc                                                                                                        >  2:111562/1‑66 (MQ=255)
                                                                                                                              gcggcCCATGTGGCCCTCCACGAAGGGCCGCATCCACACTTCTTCACCGGTGTCCGGGTcgcgcgc                                                                                                        <  1:111562/66‑1 (MQ=255)
                                                                                                                                 gcCCATGTGGCCCTCCACGAAGGGCCGCATCCACACTTCTTCACCGGTGTCCGGGTCGCGCGCGAACAGCTGGCCGACCACGCCGAATTCATCGCCGGAGCTGCCGTGGATCAGCAGCACCTTGCCGCTGGTCTTGTCCTTGATCAGCa                  >  2:119006/1‑149 (MQ=255)
                                                                                                                                             cTCCACGAAGGGCCGCATCCACACTTCTTCACCGGTGTCCGGGTCGCGCGCGAACAGCTGGCCGACCACGCCGAATTCATCGCCGGAGCTGCCGTGGATCAGCAGCACCTTGCCGCTGGTCTTGTCCTTGATc                      <  7:166899/133‑1 (MQ=255)
                                                                                                                                             cTCCACGAAGGGCCGCATCCACACTTCTTCACCGGTGTCCGGGTCGCGCGCGAACAGCTGGCCGACCACGCCGAATTCATCGCCGGAGCTGCCGTGGATCAGCAGCACCTTGCCGCTGGTCTTGTCCTTGATc                      >  8:166899/1‑133 (MQ=255)
                                                                                                                                                 aCGAAGGGCCGCATCCACACTTCTTCACCGGTGTCCGGGTCGCGCGCGAACAGCTGGCCGACCACGCCGAATTCATCGCCGGAGCTGCCGTGGATCAGCAGCACCTTGCCGCTGGTCTTGTCCTTGATCAGCACTGGCGCACCGGTCAt  >  7:240275/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CGCGCTTTGCCAAGGGGCACCGCCGCCGTGGCTCCAGGCTTCGACCTTGCCGGTTTCGGTGGTAGGGTCGTCTGGCCAGGACGGCGCCTTGACGTCACCGGTCGGGGTGCTGTCCTTGCCGTTCAGGCGGCCCATGTGGCCCTCCACGAAGGGCCGCATCCACACTTCTTCACCGGTGTCCGGGTCGCGCGCGAACAGCTGGCCGACCACGCCGAATTCATCGCCGGAGCTGCCGTGGATCAGCAGCACCTTGCCGCTGGTCTTGTCCTTGATCAGCACTGGCGCACCGGTCAT  >  NC_002947/3062583‑3062876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑