Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,079,635 C→T 9.8% intergenic (‑370/+64) PP_2688 ← / ← PP_2689 hypothetical protein/endoribonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,079,6350CT 9.8% 137.3 / 4.9 51intergenic (‑370/+64)PP_2688/PP_2689hypothetical protein/endoribonuclease
Reads supporting (aligned to +/- strand):  ref base C (22/24);  new base T (3/2);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.97e-01

CCTGGCCGCAAGGGCCGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATTTCTTCCCGGGCGAGGCCAGCCACCACTACGGCCGCGCGGTTGGGG  >  NC_002947/3079487‑3079780
                                                                                                                                                    |                                                                                                                                                  
ccTGGCCGCAAGGGCCGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAgc                                                                                                                                                    <  2:206502/149‑1 (MQ=255)
 cTGGCCGCAAGGGCCGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGAAATGGAGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAgcg                                                                                                                                                   >  2:78216/1‑149 (MQ=255)
      cGCAAGGGCCGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGATATGGGGCGCAGGCCGGTGCCTGGCGTGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCcgcg                                                                                                                                              <  3:289560/149‑1 (MQ=255)
           gggCCGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATGCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCgcgggc                                                                                                                                         <  7:86973/149‑1 (MQ=255)
               cGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCa                                                                                                                                     >  8:4315/1‑149 (MQ=255)
               cGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCa                                                                                                                                     >  5:269129/1‑149 (MQ=255)
                    cATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGa                                                                                                                                <  7:194621/149‑1 (MQ=255)
                     aTCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACt                                                                                                                                   <  4:131342/145‑1 (MQ=255)
                     aTCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACt                                                                                                                                   >  3:131342/1‑145 (MQ=255)
                     aTCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGAt                                                                                                                               >  1:38364/1‑149 (MQ=255)
                     aTCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGAt                                                                                                                               >  2:86898/1‑149 (MQ=255)
                       cAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTg                                                                                                                             >  1:130115/1‑149 (MQ=255)
                       cAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTg                                                                                                                             >  8:42667/1‑149 (MQ=255)
                         aaaaaGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTgcg                                                                                                                           >  5:27161/1‑149 (MQ=255)
                          aaaaGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTgcgc                                                                                                                          <  6:269129/149‑1 (MQ=255)
                                         aCCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGATTATCg                                                                                                           >  1:280142/1‑149 (MQ=255)
                                         aCCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCg                                                                                                           <  7:290266/149‑1 (MQ=255)
                                          cccGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGt                                                                                                          >  5:59614/1‑149 (MQ=255)
                                          cccGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGt                                                                                                          <  3:56418/149‑1 (MQ=255)
                                          cccGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGt                                                                                                          <  5:124831/149‑1 (MQ=255)
                                           ccGGGCGCGGCTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTg                                                                                                         <  8:152355/149‑1 (MQ=255)
                                            cGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTga                                                                                                        <  7:42667/149‑1 (MQ=255)
                                                              cGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGc                                                                                     <  6:27161/149‑1 (MQ=255)
                                                                    ggggCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCgcgg                                                                                                                                           >  2:84272/1‑90 (MQ=255)
                                                                    ggggCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCgcgg                                                                                                                                           <  1:84272/90‑1 (MQ=255)
                                                                     gggCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCaga                                                                               <  1:131749/148‑1 (MQ=255)
                                                                         gcAGGCCGGTGCCTGGAGGGAGAAAAAGCGAGTATTCCGGGTGCATGACGTGCGGAGGAGGGGGCATGCATCTAGCCCCGCGAGGGCGGCACACGATTGCGCGAACGCTCAATATCTTGAGA‑CGGGCGCCTTCAGCGCGTCAGaggcag                                                                          >  7:294660/1‑149 (MQ=255)
                                                                           aGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGaggcaggc                                                                        >  4:103713/1‑149 (MQ=255)
                                                                           aGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGaggcaggc                                                                        <  2:192161/149‑1 (MQ=255)
                                                                           aGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGaggcaggc                                                                        <  4:247864/149‑1 (MQ=255)
                                                                                    ccTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGaccac                                                               <  1:86898/149‑1 (MQ=255)
                                                                                            gagaAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTcca                                                       >  2:21745/1‑149 (MQ=255)
                                                                                                 aaaGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGc                                                  <  6:59614/149‑1 (MQ=255)
                                                                                                     ggAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAat                                                                                                               >  5:109671/1‑85 (MQ=255)
                                                                                                     ggAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAat                                                                                                               <  6:109671/85‑1 (MQ=255)
                                                                                                      gAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATttc                                             <  1:238643/149‑1 (MQ=255)
                                                                                                          aTTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATTTCTTcc                                         >  6:6548/1‑149 (MQ=255)
                                                                                                                 gTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGt                                                                                                          >  4:212271/1‑78 (MQ=255)
                                                                                                                       gACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATTTCTTCCCGGGCGAGgccag                            >  8:199847/1‑149 (MQ=255)
                                                                                                                          gTGGGGCGGAGGGGGCATGCATCGAGTGCCGCCCGCGCGGCGCt                                                                                                                                   >  6:158120/1‑44 (MQ=17)
                                                                                                                          gTGGGGCGGAGGGGGCATGCATCGAGTGCCGCCCGCGCGGCGCt                                                                                                                                   <  5:158120/44‑1 (MQ=17)
                                                                                                                             gggCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATTTCTTCCCGGGCGAGGCCAGccacc                       <  2:130115/148‑1 (MQ=255)
                                                                                                                                 ggAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATTTCTTCCCGGGCGAGGCCAGCCACCACTAc                  >  8:31844/1‑149 (MQ=255)
                                                                                                                                     ggggCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGGCAGAGGCAGTCGTAGACCACCAGTTCCACCAGCATTTCTTCCCGGGCGACGCCAGCCACCACTACGGcc              <  1:78216/149‑1 (MQ=255)
                                                                                                                                        gcatgcatCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATTTCTTCCCGGGCGAGGCCAGCCACCACTACGGCcgcg           <  7:31844/149‑1 (MQ=255)
                                                                                                                                          cgcaatCGAGTGCCGCCCGCGCGGCGCTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATTTCTTCCCGGGCGAGGCCAGCCACCACTACGGCcgcgcg         >  2:115122/5‑149 (MQ=255)
                                                                                                                                          atgcatCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGCCCACCAGTTCCACCAGCATTTCTTCCCGGGCGAGGCCAGCCACCCCTACGGCcgcgcg         >  7:18498/1‑149 (MQ=255)
                                                                                                                                          atgcatCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATTTCTTCCCGGGCGAGGCCAGCCACCACTACGGCcgcgcg         <  1:21745/149‑1 (MQ=255)
                                                                                                                                             aatCGAGTGCCGCCCGCGCGGCGCTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATTTCTTCCCGGGCGAg                                 <  8:229075/121‑1 (MQ=255)
                                                                                                                                             aatCGAGTGCCGCCCGCGCGGCGCTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATTTCTTCCCGGGCGAg                                 >  7:229075/2‑122 (MQ=255)
                                                                                                                                                cGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGAGCGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATTTCTTCCCGGGCGAGGCCAGCCACCACTACGGCCGCGCGGTTgg    >  1:241725/1‑149 (MQ=255)
                                                                                                                                                 gAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATTTCTTCCCGGGCGAGGCCAGCCACCACTACGGCCGCGCGGTTggg   <  2:38364/148‑1 (MQ=255)
                                                                                                                                                  aGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATTTCTTCCCGGGCGAGGCCAGCCACCACTACGGCCGCGCGGTTgggg  >  1:63530/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CCTGGCCGCAAGGGCCGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATTTCTTCCCGGGCGAGGCCAGCCACCACTACGGCCGCGCGGTTGGGG  >  NC_002947/3079487‑3079780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑