Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,079,641 G→C 10.6% intergenic (‑376/+58) PP_2688 ← / ← PP_2689 hypothetical protein/endoribonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,079,6410GC10.6% 128.9 / 7.7 47intergenic (‑376/+58)PP_2688/PP_2689hypothetical protein/endoribonuclease
Reads supporting (aligned to +/- strand):  ref base G (21/21);  new base C (3/2);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.06e-01

CGCAAGGGCCGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATTTCTTCCCGGGCGAGGCCAGCCACCACTACGGCCGCGCGGTTGGGGTAGGGTG  >  NC_002947/3079493‑3079787
                                                                                                                                                    |                                                                                                                                                   
cGCAAGGGCCGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGATATGGGGCGCAGGCCGGTGCCTGGCGTGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCcgcg                                                                                                                                                     <  3:289560/149‑1 (MQ=255)
     gggCCGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATGCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCgcgggc                                                                                                                                                <  7:86973/149‑1 (MQ=255)
         cGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCa                                                                                                                                            >  8:4315/1‑149 (MQ=255)
         cGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCa                                                                                                                                            >  5:269129/1‑149 (MQ=255)
              cATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGa                                                                                                                                       <  7:194621/149‑1 (MQ=255)
               aTCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACt                                                                                                                                          <  4:131342/145‑1 (MQ=255)
               aTCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACt                                                                                                                                          >  3:131342/1‑145 (MQ=255)
               aTCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGAt                                                                                                                                      >  1:38364/1‑149 (MQ=255)
               aTCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGAt                                                                                                                                      >  2:86898/1‑149 (MQ=255)
                 cAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTg                                                                                                                                    >  1:130115/1‑149 (MQ=255)
                 cAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTg                                                                                                                                    >  8:42667/1‑149 (MQ=255)
                   aaaaaGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTgcg                                                                                                                                  >  5:27161/1‑149 (MQ=255)
                    aaaaGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTgcgc                                                                                                                                 <  6:269129/149‑1 (MQ=255)
                                   aCCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGATTATCg                                                                                                                  >  1:280142/1‑149 (MQ=255)
                                   aCCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCg                                                                                                                  <  7:290266/149‑1 (MQ=255)
                                    cccGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGt                                                                                                                 >  5:59614/1‑149 (MQ=255)
                                    cccGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGt                                                                                                                 <  3:56418/149‑1 (MQ=255)
                                    cccGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGt                                                                                                                 <  5:124831/149‑1 (MQ=255)
                                     ccGGGCGCGGCTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTg                                                                                                                <  8:152355/149‑1 (MQ=255)
                                      cGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTga                                                                                                               <  7:42667/149‑1 (MQ=255)
                                                        cGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGc                                                                                            <  6:27161/149‑1 (MQ=255)
                                                              ggggCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCgcgg                                                                                                                                                  <  1:84272/90‑1 (MQ=255)
                                                              ggggCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCgcgg                                                                                                                                                  >  2:84272/1‑90 (MQ=255)
                                                               gggCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCaga                                                                                      <  1:131749/148‑1 (MQ=255)
                                                                   gcAGGCCGGTGCCTGGAGGGAGAAAAAGCGAGTATTCCGGGTGCATGACGTGCGGAGGAGGGGGCATGCATCTAGCCCCGCGAGGGCGGCACACGATTGCGCGAACGCTCAATATCTTGAGA‑CGGGCGCCTTCAGCGCGTCAGaggcag                                                                                 >  7:294660/1‑149 (MQ=255)
                                                                     aGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGaggcaggc                                                                               <  4:247864/149‑1 (MQ=255)
                                                                     aGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGaggcaggc                                                                               <  2:192161/149‑1 (MQ=255)
                                                                     aGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGaggcaggc                                                                               >  4:103713/1‑149 (MQ=255)
                                                                              ccTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGaccac                                                                      <  1:86898/149‑1 (MQ=255)
                                                                                      gagaAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTcca                                                              >  2:21745/1‑149 (MQ=255)
                                                                                           aaaGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGc                                                         <  6:59614/149‑1 (MQ=255)
                                                                                               ggAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAat                                                                                                                      <  6:109671/85‑1 (MQ=255)
                                                                                               ggAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAat                                                                                                                      >  5:109671/1‑85 (MQ=255)
                                                                                                gAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATttc                                                    <  1:238643/149‑1 (MQ=255)
                                                                                                    aTTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATTTCTTcc                                                >  6:6548/1‑149 (MQ=255)
                                                                                                           gTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGt                                                                                                                 >  4:212271/1‑78 (MQ=255)
                                                                                                                 gACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATTTCTTCCCGGGCGAGgccag                                   >  8:199847/1‑149 (MQ=255)
                                                                                                                    gTGGGGCGGAGGGGGCATGCATCGAGTGCCGCCCGCGCGGCGCt                                                                                                                                          <  5:158120/44‑1 (MQ=17)
                                                                                                                    gTGGGGCGGAGGGGGCATGCATCGAGTGCCGCCCGCGCGGCGCt                                                                                                                                          >  6:158120/1‑44 (MQ=17)
                                                                                                                       gggCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATTTCTTCCCGGGCGAGGCCAGccacc                              <  2:130115/148‑1 (MQ=255)
                                                                                                                           ggAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATTTCTTCCCGGGCGAGGCCAGCCACCACTAc                         >  8:31844/1‑149 (MQ=255)
                                                                                                                               ggggCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGGCAGAGGCAGTCGTAGACCACCAGTTCCACCAGCATTTCTTCCCGGGCGACGCCAGCCACCACTACGGcc                     <  1:78216/149‑1 (MQ=255)
                                                                                                                                  gcatgcatCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATTTCTTCCCGGGCGAGGCCAGCCACCACTACGGCcgcg                  <  7:31844/149‑1 (MQ=255)
                                                                                                                                    cgcaatCGAGTGCCGCCCGCGCGGCGCTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATTTCTTCCCGGGCGAGGCCAGCCACCACTACGGCcgcgcg                >  2:115122/5‑149 (MQ=255)
                                                                                                                                    atgcatCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGCCCACCAGTTCCACCAGCATTTCTTCCCGGGCGAGGCCAGCCACCCCTACGGCcgcgcg                >  7:18498/1‑149 (MQ=255)
                                                                                                                                    atgcatCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATTTCTTCCCGGGCGAGGCCAGCCACCACTACGGCcgcgcg                <  1:21745/149‑1 (MQ=255)
                                                                                                                                       aatCGAGTGCCGCCCGCGCGGCGCTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATTTCTTCCCGGGCGAg                                        >  7:229075/2‑122 (MQ=255)
                                                                                                                                       aatCGAGTGCCGCCCGCGCGGCGCTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATTTCTTCCCGGGCGAg                                        <  8:229075/121‑1 (MQ=255)
                                                                                                                                          cGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGAGCGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATTTCTTCCCGGGCGAGGCCAGCCACCACTACGGCCGCGCGGTTgg           >  1:241725/1‑149 (MQ=255)
                                                                                                                                           gAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATTTCTTCCCGGGCGAGGCCAGCCACCACTACGGCCGCGCGGTTggg          <  2:38364/148‑1 (MQ=255)
                                                                                                                                            aGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATTTCTTCCCGGGCGAGGCCAGCCACCACTACGGCCGCGCGGTTgggg         >  1:63530/1‑148 (MQ=255)
                                                                                                                                                  gcgcgGGCGGCACTCGATTGCGCGATCGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATTTCTTCCCGGGCGAGGCCAGCCACCACTACGGCCGCGCGGTTGGGGTAGGGTg  >  3:148736/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGCAAGGGCCGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTGAACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGA‑CGGGCGCCTTCAGGGCGTCAGAGGCAGGCGTAGACCACCAGTTCCACCAGCATTTCTTCCCGGGCGAGGCCAGCCACCACTACGGCCGCGCGGTTGGGGTAGGGTG  >  NC_002947/3079493‑3079787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑