Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,181,065 T→C 7.3% W88R (TGG→CGG)  PP_2791 → aminoglycoside phosphotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,181,0650TC 7.3% 159.1 / 3.0 55W88R (TGG→CGG) PP_2791aminoglycoside phosphotransferase
Reads supporting (aligned to +/- strand):  ref base T (25/26);  new base C (2/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

GCCATGACTGACGATCACCGCCTGGCCGAGTTCATCCGCGAGCAGGCCTCGGCAACGCGGGTGGTCATCCAGGCGCGCAAGCGCCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGTGAACAGGAGTTCGCCGTGCTGCAGGTGGTTTACCAGGCCGGCGTGAAAGTGCCACGCCCGCTCTGGCTGT  >  NC_002947/3180921‑3181203
                                                                                                                                                |                                                                                                                                           
gCCATGACTGACGATCACCGCCTGGCCGAGTTCATCCGCGAGCAGGCCTCGGCAACGCGGGTGGTCATCCTGGCGCGCAAGCGCCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGc                                                                                                                                         <  2:95142/149‑1 (MQ=255)
 ccATGACTGACGATCACCGCCTGGCCGAGTTCATCCGCGAGCAGGCCTCGGCAACGCGGGTGGTCATCCAGGCGCGCAAGCGCCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGcc                                                                                                                                        <  5:136504/149‑1 (MQ=255)
  cATGACTGACGATCACCGCCTGGCCGAGTTCATCCGCGAGCAGGCCGCGGCAACGCGGGTGGTCATCCAGGCGCGCAAGCGCCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCg                                                                                                                                       <  4:304794/149‑1 (MQ=255)
         gacGATCACCGCCTGGCCGAGTTCATCCGCGAGCAGGCCTCGGCAACGCGGGTGGTCATCCAGGCGCGCAAGCGCCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTcc                                                                                                                                >  6:71464/1‑148 (MQ=255)
         gacGATCACCGCCTGGCCGAGTTCATCCGCGAGCAGGCCTCGGCAACGCGGGTGGTCATCCAGGCGCGCAAGCGCCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTcc                                                                                                                                <  5:71464/148‑1 (MQ=255)
          acGATCACCGCCTGGCCGAGTTCATCCGCGAGCAGGCCTCGGCAACGCGGGTGGTCATCCAGGCGCGCAAGCGCCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTcc                                                                                                                                <  7:147527/147‑1 (MQ=255)
               cACCGCCTGGCCGAGTTCATCCGCGAGCAGGCCTCGGCAACGCGGGTGGTCATCCAGGCGCGCAAGCGCCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTg                                                                                                                         >  2:63904/1‑149 (MQ=255)
                  cGCCTGGCCGAGTTCATCCGCGAGCAGGCCTCGGCAACGCGGGTGGTCATCCAGGCGCGCAAGCGCCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTggg                                                                                                                       >  5:58402/1‑148 (MQ=255)
                   gCCTGGCCGAGTTCATCCGCGAGCAGGCCTCGGCAACGCGGGTGGTCATCCAGGCGCGCAAGCGCCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTa                                                                                                                     <  6:58402/149‑1 (MQ=255)
                   gCCTGGCCGAGTTCATCCGCGAGCAGGCCTCGGCAACGCGGGTGGTCATCCAGGCGCGCAAGCGCCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTa                                                                                                                     <  1:164768/149‑1 (MQ=255)
                         ccGAGTTCATCCGCGAGCAGGCCTCGGCAACGCGGGTGGTCATCCAGGCGCGCAAGCGCCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGc                                                                                                                             <  3:303437/135‑1 (MQ=255)
                         ccGAGTTCATCCGCGAGCAGGCCTCGGCAACGCGGGTGGTCATCCAGGCGCGCAAGCGCCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGc                                                                                                                             >  4:303437/1‑135 (MQ=255)
                          cGAGTTCATCCGCGAGCAGGCCTCGGCAACGCGGGTGGTCATCCAGGCGCGCAAGCGCCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTgg                                                                                                                        <  2:132241/139‑1 (MQ=255)
                          cGAGTTCATCCGCGAGCAGGCCTCGGCAACGCGGGTGGTCATCCAGGCGCGCAAGCGCCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTgg                                                                                                                        >  1:132241/1‑139 (MQ=255)
                           gAGTTCATCCGCGAGCTGGCCTCGGCAACGGGGGTGGTAATCCAGGCGCGCAAGCGCCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGcc                                                                                                                                        <  2:149944/123‑1 (MQ=255)
                           gAGTTCATCCGCGAGCAGGCCTCGGCAACGCGGGTGGTCATCCAGGCGCGCAAGCGCCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGcc                                                                                                                                        >  1:149944/1‑123 (MQ=255)
                           gAGTTCATCCGCGAGCAGGCCTCGGCAACGCGGGTGGTCATCCAGGCGCGCAAGCGCCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAg                                                                                                             >  1:134395/1‑149 (MQ=255)
                                          cAGGCCTCGGCAACGCGGGTGGTCATCCAGGCGCGCAAGCGCCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAgc                                                                                              >  4:61374/1‑149 (MQ=255)
                                          cAGGCCTCGGCAACGCGGGTGGTCATCCAGGCGCGCAAGCGCCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAgc                                                                                              >  4:187253/1‑149 (MQ=255)
                                            ggCCTCGGCAACGCGGGTGGTCATCCAGGCGCGCAAGCGCCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAgcgc                                                                                            >  1:82390/1‑149 (MQ=255)
                                               cTCGGCAACGCGGGTGGTCATCCAGGCGCGCAAGCGCCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTAc                                                                                         >  5:298463/1‑149 (MQ=255)
                                                        gcgGGTGGTCATCCAGGCGCGCAAGCGCCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGcc                                                                                <  7:246371/149‑1 (MQ=255)
                                                             tggtCATCCAGGCGCGCAAGCGCCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGAc                                                                           <  2:192537/149‑1 (MQ=255)
                                                               gtCATCCAGGCGCGCAAGCGCCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGAcc                                                                          <  2:134395/148‑1 (MQ=255)
                                                                tCATCCAGGCGCGCAAGCGCCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGt                                                                        >  8:171180/1‑149 (MQ=255)
                                                                      aGGCGCGCAAGCGCCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGTGAACAg                                                                  >  3:267080/1‑149 (MQ=255)
                                                                             cAAGCGCCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTAGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGTGAACAGGAGTTCg                                                           >  3:76470/1‑149 (MQ=255)
                                                                                gcgcCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGTGAACAGGAGTACTCCg                                                        >  8:187502/1‑149 (MQ=255)
                                                                                  gcCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGTGAACAGGAGTTCGCCGtg                                                      >  8:19897/1‑149 (MQ=255)
                                                                                   ccTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGTGAACAGGAGTTCGCCGtgc                                                     >  4:93071/1‑149 (MQ=255)
                                                                                   ccTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGTGAACAGGAGTTCGCCGtgc                                                     <  4:141047/149‑1 (MQ=255)
                                                                                     tGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGTGAACAGGAGTTCGCCGtgctg                                                   >  3:1086/1‑149 (MQ=255)
                                                                                          ggcggcgCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGTGAACAGGAGTTCGCCGTGCTGCAggt                                              <  7:171180/149‑1 (MQ=255)
                                                                                                      cAGGAATACTGGCTGCTGGACCTGCGGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGTGAACAGGAGTTCGCCGTGCTGCAGGTGGTTTACCAg                                    >  5:333447/1‑147 (MQ=255)
                                                                                                      cAGGAAAACTGGCTGCTTGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGTGAACAGGAGTTCGCCGTGCTGCAGGGGGTTTACAAg                                    <  6:333447/147‑1 (MQ=255)
                                                                                                      cAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTggg                                                                                                                       <  4:85164/64‑1 (MQ=255)
                                                                                                      cAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTggg                                                                                                                       >  3:85164/1‑64 (MQ=255)
                                                                                                      cAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGTGAACAGGAGTTCGCCGTGCTGCAGGTGGTTTACCAgg                                   <  3:61374/148‑1 (MQ=255)
                                                                                                      cAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGTGAACAGGAGTTCGCCGTGCTGCAGGTGGTTTACCAgg                                   <  3:187253/148‑1 (MQ=255)
                                                                                                         gAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGTGAACAGGAGTTCGCCGTGCTGCAGGTGGTTTACCAGGCCgg                               <  4:1086/149‑1 (MQ=255)
                                                                                                           aaaCTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGGCTGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGTGAACAGGa                                                                >  1:229078/1‑115 (MQ=255)
                                                                                                           aaaCTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGTGAACAGGa                                                                <  2:229078/114‑1 (MQ=255)
                                                                                                                     ctgGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGTGAACAGGAGTTCGCCGTGCTGCAGGTGGTTTACCAGGCCGGCGTGAAAGTGc                    >  6:36245/1‑148 (MQ=255)
                                                                                                                          ccTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGTGAACAGGAGTTCGCCGTGCTGCAGGTGGTTTACCAGGCCGGCGTGAAAGTGCCACGcc              <  5:36245/149‑1 (MQ=255)
                                                                                                                          ccTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGTGAACAGGAGTTCGCCGTGCTGCAGGTGGTTTACCAGGCCGGCGTGAAAGTGCCACGcc              <  1:63904/149‑1 (MQ=255)
                                                                                                                             gctgATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGTGAACAGGAGTTCGCCGTGCTGCAGGTGGTTTACCAGGCCGGCGTGAAAGTGCCACGCCCGc           >  1:98473/1‑149 (MQ=255)
                                                                                                                                  tCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGTGAACAGGAGTTCGCCGTGCTGCAGGTGGTTTACCAGGCCGGCGTGAAAGTGCCACGCCCGCTCTgg      >  3:95431/1‑149 (MQ=255)
                                                                                                                                    gacaccggcCCACGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGTGAACAGGAGTTCGCCGTGCTGCAGGTGGTTTACCAGGCCGGCGTGAAAGTGCCAc                 >  5:12216/6‑136 (MQ=255)
                                                                                                                                    gacaccggcCCACGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGTGAACAGGAGTTCGCCGTGCTGCAGGTGGTTTACCAGGCCGGCGTGAAAGTGCCAc                 <  6:12216/131‑1 (MQ=255)
                                                                                                                                    gacaccggcCCACGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGTGAACAGGAGTTCGCCGTGCTGCAGGTGGTTTACCAGGCCGGCGTGAAAGTGCCAc                 >  1:49263/6‑136 (MQ=255)
                                                                                                                                    gacaccggcCCACGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGTGAACAGGAGTTCGCCGTGCTGCAGGTGGTTTACCAGGCCGGCGTGAAAGTGCCAc                 <  2:49263/131‑1 (MQ=255)
                                                                                                                                    gAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGTGAACAGGAGTTCGCCGTGCTGCAGGTGGTTTACCAGGCCGGCGTGAAAGTGCCACGCCCGCTCTGGc     <  4:76470/148‑1 (MQ=255)
                                                                                                                                     aaGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGTGAACAGGAGTTCGCCGTGCTGCAGGTGGTTTACCAGGCCGGCg                             <  3:120224/123‑1 (MQ=255)
                                                                                                                                     aaGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGTGAACAGGAGTTCGCCGTGCTGCAGGTGGTTTACCAGGCCGGCg                             >  4:120224/1‑123 (MQ=255)
                                                                                                                                      aGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGTGAACAGGAGTTCGCCGTGCTGCAGGTGGTTTACCAGGCCGGCGTGAAAGTGCCACGCCCGCTCTGGCtgt  <  6:298463/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                           
GCCATGACTGACGATCACCGCCTGGCCGAGTTCATCCGCGAGCAGGCCTCGGCAACGCGGGTGGTCATCCAGGCGCGCAAGCGCCTGAGCGGCGGCGCTATCCAGGAAAACTGGCTGCTGGACCTGCTGATCGAAGGCGGCCCGTGGGCCGG‑TGTCCGGCGTTGGGTACTGCGCAGCGATGCGCTTTCAGCGCTACCCGCCAGCCTTGACCGTGAACAGGAGTTCGCCGTGCTGCAGGTGGTTTACCAGGCCGGCGTGAAAGTGCCACGCCCGCTCTGGCTGT  >  NC_002947/3180921‑3181203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑