Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,189,987	G→A	13.6%	F221F (TTC→TTT) ‡	PP_2799 ←	class III aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,189,987	0	G	A	13.6%	106.4 / 8.1	44	F221F (TTC→TTT) ‡	PP_2799	class III aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (18/20);  new base A (4/2);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.45e-01

GAACATTGTGCCAAGCCTGCCAAAGCCGGTGATGATTTCGTCGATCACCACGAGGATGTCGTAGCGTTTGCACACCGCCTGGATCGCCGCCCAGTAGCCTGTGGGAGGAGGGATCACG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCCAGACGGACCGTAAAGGCCTCTTGCGATTCTCCTGGCCGAGCAAATCGGTAGAAGTTCGGGCAGGTCAGGTGATGAACCGGGATGGCC  >  NC_002947/3189840‑3190131                                                                                                                                                      |                                                                                                                                                 gAACATTGTGCCAAGCCTGCCAAAGCCGGTGATGATTTCGTCGATCACCACGAGGATGTCGTAGCGTTTGCACACCGCCTGGATCGCCGCCCAGTAGCCTGTGGGAGGAGGGATCACG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATg                                                                                                                                                  >  7:271114/1‑148 (MQ=255)     aTTGTGCCAAGCCTGCCAAAGCCGGTGATGATTTCGTCGATCACCACGAGGATGTCGTAGCGTTTGCACACCGCCTGGATCGCCGCCCAGTAGCCTGTGGGAGGAGGGATCACG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAAcgc                                                                                                                                             <  7:43165/149‑1 (MQ=255)                    ccAAAGCCGGTGATGATTTCGTCGATCACCACGAGGATGTCGTAGCGTTTGCACACCGCCTGGATCGCCGCCCAGTAGCCTGTGGGAGGAGGGATCACG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAACGCGCCGCTGGTTTCCgg                                                                                                                              >  8:112261/1‑149 (MQ=255)                           cGGTGATGATTTCGTCGATCACCACGAGGATGTCGTAGCGTTTGCACACCGCCTGGATCGCCGCCCAGTAGCCTGTGGGAGGAGGGATCACG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCg                                                                                                                       >  2:193459/1‑149 (MQ=255)                                atgatTTCGTCGATCACCACGAGGATGTCGTAGCGTTTGCACACCGCCTGGATCGCCGCCCAGTAGCCTGTGGGAGGAGGGATCACG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAg                                                                                                                  >  5:6053/1‑149 (MQ=255)                                 tgatTTCGTCGATCACCACGAGGATGTCGTAGCGTTTGCACACCGCCTGGATCGCCGCCCAGTAGCCTGTGGGAGGAGGGATCACGCCCCACCTGCCGCGATTACCGGCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCaa                                                                                                                   <  1:92644/149‑1 (MQ=255)                                  gatTTCGTCGATCACCACGAGGATGTCGTAGCGTTTGCACACCGCCTGGATCGCCGCCCAGTAGCCTGTGGGAGGAGGGATCACG‑‑CCACCTGCCGCGTTCATCGGCGAGCCGGTAATCgcggcagg                                                                                                                                       >  6:65518/1‑123 (MQ=37)                                  gatTTCGTCGATCACCACGAGGATGTCGTAGCGTTTGCACACCGCCTGGATCGCCGCCCAGTAGCCTGTGGGAGGAGGGATCACG‑‑CCACCTGCCGCGTTCATCGGCGAGCCGGTAATCgcggcagg                                                                                                                                       <  5:65518/126‑4 (MQ=37)                                           gATCACCACGAGGATGTCGTAGCGTTTGCACACCGCCTGGATCGCCGCCCAGTAGCCTGTGGGAGGAGGGATCACG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGtt                                                                                                       >  5:290956/1‑149 (MQ=255)                                                ccacGAGGATGTCGTAGCGTTTGCACACCGCCTGGATCGCCGCCCAGTAGCCTGTGGGAGGAGGGATCACG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAgt                                                                                                  <  4:305709/149‑1 (MQ=255)                                                       gATGTCGTAGCGTTTGCACACCGCCTGGATCGCCGCCCAGTAGCCTGTGGGAGGAGGGATCACG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGttcgttcg                                                                                           >  4:103833/1‑149 (MQ=255)                                                         tGTCGTAGCGTTTGCACACCGCCTGGATCGCCGCCCAGTAGCCTGTGGGAGGAGGGATCACG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGcc                                                                                         <  1:193459/149‑1 (MQ=255)                                                                  gTTTGCACACCGCCTGGATCGCCGCCCAGTAGCCTGTGGGAGGAGGGATCACG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCCAGACGGAcc                                                                                >  5:163378/1‑149 (MQ=255)                                                                   tttGCACACCGCCTGGATCGCCGCCCAGTAGCCTGTGGGAGGAGGGATCACG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCCAGACGGACCg                                                                               <  6:6053/149‑1 (MQ=255)                                                                     tGCACACCGCCTGGATCGCCGCCCAGTAGCCTGTGGGAGGAGGGATCACG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAATGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCAAGACGGACCGt                                                                              >  1:147758/1‑148 (MQ=255)                                                                     tGCACACCGCCTGGATCGCCGCCCAGTAGCCTGTGGGAGGAGGGATCACG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAACGCGGCGATAGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCCAGACGGACCGt                                                                              >  4:114995/1‑148 (MQ=255)                                                                        acacCGCCTGGATCGCCGCCCAGTAGCCTGTGGGAGGAGGGATCACG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCCAGACGGACCGTAAAg                                                                          <  8:271114/149‑1 (MQ=255)                                                                          acCGCCTGGATCGCCGCCCAGTAGCCTGTGGGAGGAGTGATCACG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAACGCGGCGATGTTTTCCGGCCCTTCGGCAAGTATATAGCGTTCCAGTTCGTTCGCCAGACGTACCGTAAAGGc                                                                        <  2:17175/149‑1 (MQ=255)                                                                          acCGCCTGGATCGCCGCCCAGTAGCCTGTGGGAGGAGGGATCACG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAgttc                                                                                                <  4:209717/125‑1 (MQ=255)                                                                          acCGCCTGGATCGCCGCCCAGTAGCCTGTGGGAGGAGGGATCACG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAgttc                                                                                                >  3:209717/1‑125 (MQ=255)                                                                                     cgccgcCCAGTAGCCTGTGGGAGGAGGGATCACG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCCAGACGGACCGTAAAGGCCTCTTGCGAt                                                              >  4:16269/1‑148 (MQ=255)                                                                                           ccAGTAGCCTGTGGGAGGAGGGATCACG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCCAGACGGACCGTAAAGGCCTCTTGCGATTCTCCTg                                                       <  2:222319/149‑1 (MQ=255)                                                                                           ccAGTAGCCTGTGGGAGGAGGGATCACG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCCAGACGGACCGTAAAGGCCTCTTGCGATTCTCCTg                                                       <  2:50258/149‑1 (MQ=255)                                                                                                    tgtgGGAGGAGGGATCACG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCCAGACGGACCGTAAAg                                                                          <  8:43619/121‑1 (MQ=255)                                                                                                    tgtgGGAGGAGGGATCACG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCCAGACGGACCGTAAAg                                                                          >  7:43619/1‑121 (MQ=255)                                                                                                    tgtgGGAGGAGGGATCACG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCCAGACGGACCGTAAAGGCCTCTTGCGATTCTCCTGGCCGAGCa                                               >  2:258059/1‑148 (MQ=255)                                                                                                              tggATCACG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAACGCGGCTATGGTTTCCGGCCCTTCGGCAATGATATAGCGTTCCAGTTCGTTCGCCAGACGGACCGTAAAGGCCTCTTGCGATTCTCCTGGCCGAGCAAATCTGTAGaa                                    <  7:182241/148‑1 (MQ=255)                                                                                                               ggATCACG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCCAGACGGACCGTAAAGGCCTCTTGCGATTCTCCTGGCCGAGCAAATCGGTAGAAg                                   >  6:276803/1‑149 (MQ=255)                                                                                                                gATCACG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCCAGACGGACCGTAAAGGCCTCTTGCGATTCTCCTGGCCGAGCAAATCGGTAGAAGt                                  >  2:176829/1‑149 (MQ=255)                                                                                                                    aCG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCCAGACGGACCGTAAAGGCCTCTTGCGATTCTCCTGGCCGAGCAAATCGGTAGAAGTTCgg                              >  8:112638/1‑149 (MQ=255)                                                                                                                    aCG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCCAGACGGACCGTAAAGGCCTCTTGCGATTCTCCTGGCCGAGCAAATCGGTAGAAGTTCg                               <  4:242641/148‑1 (MQ=255)                                                                                                                     cG‑‑CCACCTGCCGCGATTACCTGCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCCAGACGGACCGTAAAGGCCTCTTGCg                                                                <  5:563/114‑1 (MQ=255)                                                                                                                     cG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCCAGACGGACCGTAAAGGCCTCTTGCg                                                                >  6:563/1‑114 (MQ=255)                                                                                                                     cG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCCAGACGGACCGTAAAGGCCTCTTGCGATTCTCCTGGCCGAGCAAATCGGTAGAAGTACggg                             <  3:16269/149‑1 (MQ=255)                                                                                                                          ccatcgCCGCGTTCATCGGCGAGCCGGTAATCGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCCAGACGGACCGTAAAGGCCTCTTGCGATTCTCCTGGCCGAGCAAATCGGTAGAAGTTCGGGcag                          >  3:163606/6‑149 (MQ=38)                                                                                                                           catcgCCGCGTTCATCGGCGAGCCGGTAATCGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCCAGACGGACCGTAAAGGCCTCTTGCGATTCTCCTGGCCGAGCAAATCGGTAGAAGTTCGGGc                            <  3:247031/142‑1 (MQ=38)                                                                                                                           catcgCCGCGTTCATCGGCGAGCCGGTAATCGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCCAGACGGACCGTAAAGGCCTCTTGCGATTCTCCTGGCCGAGCAAATCGGTAGAAGTTCGGGc                            >  4:247031/5‑146 (MQ=38)                                                                                                                            atcgCCGCGTTCATCGGCGAGCCGGTAATCGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCCAGACGGACCGTAAAGGCCTCTTGCGATTCTCCTGGCCGAGCAAATCGGTAGAAGTTCGGGcaggt                        >  3:144539/4‑149 (MQ=38)                                                                                                                                ccGCGATTACCGGCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCCAGACGGACCGTAAAGGCCTCTTGCGATTCTCCTGGCCGAGCAAATCGGTAGAAGTTCGGGcaggtcag                     <  7:112638/148‑1 (MQ=255)                                                                                                                                       tACCGGCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCCAGACGGACCGTAAAGGCCTCtt                                                                   <  3:154388/95‑1 (MQ=255)                                                                                                                                       tACCGGCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCCAGACGGACCGTAAAGGCCTCtt                                                                   >  4:154388/1‑95 (MQ=255)                                                                                                                                            gCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCCAGACGGACCGTAAAGGCCTCTTGCGATTCTCCTGGCCGAGCAAATCGGTAGAAGTTCgg                              <  2:47611/127‑1 (MQ=255)                                                                                                                                            gCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCCAGACGGACCGTAAAGGCCTCTTGCGATTCTCCTGGCCGAGCAAATCGGTAGAAGTTCgg                              >  1:47611/1‑127 (MQ=255)                                                                                                                                                gCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCCAGACGGACCGTAAAGGCCTCTTGCGATTCTCCTGGCCGAGCAAATCGGTAGAAGTTCGGGCAGGTCAGGTGATGAACCGGGATg     <  5:276803/148‑1 (MQ=255)                                                                                                                                                  cGATGAATGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCCAGAGGGACCGTAAAGGGCTCTAGCGATTCTCCTGGCCGAGCAAAGCGGTAGAAGTTCGGGCAGGTCAGGTGATGAACAGGGATGGcc  <  2:147758/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                 GAACATTGTGCCAAGCCTGCCAAAGCCGGTGATGATTTCGTCGATCACCACGAGGATGTCGTAGCGTTTGCACACCGCCTGGATCGCCGCCCAGTAGCCTGTGGGAGGAGGGATCACG‑‑CCACCTGCCGCGATTACCGGCTCGCCGATGAACGCGGCGATGGTTTCCGGCCCTTCGGCAAGGATATAGCGTTCCAGTTCGTTCGCCAGACGGACCGTAAAGGCCTCTTGCGATTCTCCTGGCCGAGCAAATCGGTAGAAGTTCGGGCAGGTCAGGTGATGAACCGGGATGGCC  >  NC_002947/3189840‑3190131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑