Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 308,738 C→T 9.4% intergenic (+1753/‑116) hslO → / → PP_0254 molecular chaperone HslO/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947308,7380CT 9.4% 146.4 / 4.7 53intergenic (+1753/‑116)hslO/PP_0254molecular chaperone HslO/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (23/25);  new base T (2/3);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.00e-01

GGCTGACAAAGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCAC  >  NC_002947/308591‑308876
                                                                                                                                                   |                                                                                                                                          
ggCTGACAAAGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGTAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGGCGccccc                                                                                                                                            <  8:268841/148‑1 (MQ=255)
      cAAAGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGggcg                                                                                                                                     >  6:333269/1‑149 (MQ=255)
        aaGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGggcggc                                                                                                                                   >  4:15029/1‑149 (MQ=255)
              ccTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCTGGGGGCGGCtttctc                                                                                                                             <  2:6158/149‑4 (MQ=255)
               cTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTtg                                                                                                                            >  2:274737/1‑149 (MQ=255)
                tACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCGGTAAGCCGAGCTCAG‑CACAACGAAAAAGCCGCCCCTGGGGGCGGCtttctcgtt                                                                                                                          <  8:272413/149‑7 (MQ=255)
                  cgacgcGGCTGCCAAAGGTCTGGCAAATCGGTTCATCGAAAACTTCAAGAATTTCGAAGTTTCCGACGCCATCAATGCCGCTGGCCCGCAGCTTTAAGCCGAGCTCAG‑AACAACTAAAAAGCCGCACCTGGGGGCGGCtttctcgtt                                                                                                                          <  8:276665/147‑7 (MQ=255)
                  cgacgaGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTgg                                                                                                                         <  1:274737/149‑1 (MQ=255)
                  cgacgaGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTgg                                                                                                                         <  8:238621/149‑1 (MQ=255)
                        ggCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAgg                                                                                                                   <  2:272873/149‑1 (MQ=255)
                         gCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAggg                                                                                                                  >  2:139823/1‑149 (MQ=255)
                          cTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGc                                                                                                                 <  2:87790/149‑1 (MQ=255)
                               aaaGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGcc                                                                                                            >  4:30862/1‑149 (MQ=255)
                                        gCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGaa                                                                                                      <  5:333269/146‑1 (MQ=255)
                                        gCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAg                                                                                                     <  3:30862/147‑1 (MQ=255)
                                             gCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTgg                                                                                                                         >  7:99816/1‑122 (MQ=255)
                                             gCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTgg                                                                                                                         <  8:99816/122‑1 (MQ=255)
                                                gTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCAt                                                                                           >  4:32489/1‑149 (MQ=255)
                                                     tCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAgg                                                                                        >  4:37651/1‑147 (MQ=255)
                                                      cGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAg                                                                                     >  7:41363/1‑149 (MQ=255)
                                                             ttCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGt                                                                              >  6:204945/1‑149 (MQ=255)
                                                             ttCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGt                                                                              >  5:248165/1‑149 (MQ=255)
                                                                aagaagTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTg                                                                           <  5:320451/149‑1 (MQ=255)
                                                                aagaagTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGG‑‑GGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTgg                                                                          <  3:162434/148‑1 (MQ=255)
                                                                      ttCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGttttt                                                                     <  1:139823/149‑1 (MQ=255)
                                                                          aaGTTTCCGACGCCATCAAGGCCGCTGGCTCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAg                                                                                     <  6:216294/129‑1 (MQ=255)
                                                                          aaGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGt                                                                              <  8:60682/136‑1 (MQ=255)
                                                                          aaGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGt                                                                 >  1:125306/1‑149 (MQ=255)
                                                                          aaGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCAGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGt                                                                              >  7:60682/1‑136 (MQ=255)
                                                                          aaGTTTCCGACGCCATCAAGGCCGCTGGCACGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAg                                                                                     >  5:216294/1‑129 (MQ=255)
                                                                            gTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGttt                                                               <  3:15029/149‑1 (MQ=255)
                                                                                  gACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGACTCGTTTTTCTGTTTCACTGt                                                         >  2:118364/1‑149 (MQ=255)
                                                                                   aCGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCt                                                                   <  3:84955/138‑1 (MQ=255)
                                                                                   aCGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCt                                                                   >  4:84955/1‑138 (MQ=255)
                                                                                           aaGGCCGCTGGCCCGCATCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGTTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAATTCTGGTTTTTCTGTTTCACTGTCAGCATcc                                                 <  6:68190/148‑1 (MQ=255)
                                                                                              gCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTg                                             <  6:248165/149‑1 (MQ=255)
                                                                                               ccGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGc                                            <  5:204945/149‑1 (MQ=255)
                                                                                                   tGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCg                                           >  2:91721/1‑146 (MQ=255)
                                                                                                     gCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCa                                                             >  4:109558/1‑126 (MQ=255)
                                                                                                         gCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTg                                             >  6:283063/1‑138 (MQ=255)
                                                                                                         gCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTg                                             <  5:283063/138‑1 (MQ=255)
                                                                                                         gCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGCTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGGCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAaga                                  <  6:154537/149‑1 (MQ=255)
                                                                                                              tGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGagccag                             >  1:304324/1‑149 (MQ=255)
                                                                                                                      gAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGa                     <  8:41363/149‑1 (MQ=255)
                                                                                                                            aGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAg               <  3:37651/149‑1 (MQ=255)
                                                                                                                                 ccacaaaaaaGCCGCCCCTGGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCt          >  3:87883/8‑149 (MQ=255)
                                                                                                                                      aaaaaGCCGCCACTGGGTGCGGCTTTTTAATGGCATAGCGCATGCCCGCGAAGGGGCACGCATAGGAAGAGAAAGTCTGGATTATCTTTTTAACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGAACACCGAAGCCCCTCcgcg     >  6:158116/3‑149 (MQ=255)
                                                                                                                                        aaaGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGagccagc                            <  2:153586/124‑1 (MQ=255)
                                                                                                                                        aaaGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGagccagc                            >  1:153586/1‑124 (MQ=255)
                                                                                                                                         aaGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCAc  <  2:304324/149‑1 (MQ=255)
                                                                                                                                           gccgccCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAagag                                 <  6:149050/116‑1 (MQ=255)
                                                                                                                                           gccgccCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAagag                                 >  5:149050/1‑116 (MQ=255)
                                                                                                                                            ccgccCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAgc      >  2:77364/1‑142 (MQ=255)
                                                                                                                                            ccgccCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAgc      <  1:77364/142‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                          
GGCTGACAAAGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCAC  >  NC_002947/308591‑308876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑