Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,209,677	T→A	5.9%	F301I (TTC→ATC) ‡	PP_2813 →	BNR domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,209,677	0	T	A	5.9%	209.3 / 2.6	68	F301I (TTC→ATC) ‡	PP_2813	BNR domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (32/32);  new base A (2/2);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01

GGCTCTGTCACTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGGGTGGTCGT  >  NC_002947/3209536‑3209820                                                                                                                                              |                                                                                                                                                 ggCTCTGTCACTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGc                                                                                                                                           <  7:134198/149‑1 (MQ=255)  gCTCTGTCACTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTc                                                                                                                                                <  4:167957/143‑1 (MQ=255)  gCTCTGTCACTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTc                                                                                                                                                >  3:167957/1‑143 (MQ=255)   ctctGTCACTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGcc                                                                                                                                          >  6:124509/1‑148 (MQ=255)   ctctGTCACTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGAACAACGCCg                                                                                                                                         <  5:271920/149‑1 (MQ=255)      tGTCACTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAg                                                                                                                                      >  6:177563/1‑149 (MQ=255)      tGTCACTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAg                                                                                                                                      >  4:200138/1‑149 (MQ=255)                         gCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGtttt                                                                                                                    <  5:124509/148‑1 (MQ=255)                              ctctCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGcc                                                                                                              <  5:3241/149‑1 (MQ=255)                                   gtgtGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGAGGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAggg                                                                                                         <  4:203190/149‑1 (MQ=255)                                    tgtgCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAgggg                                                                                                        <  5:153404/149‑1 (MQ=255)                                            cGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGccc                                                                                               <  1:116836/149‑1 (MQ=255)                                            cGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGccc                                                                                               >  2:550/1‑149 (MQ=255)                                                 gggCTGGATGGGCTTAGCGTGCAGAGCAGCGAGGGCGGGCACAGATTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCa                                                                                          <  4:8245/149‑1 (MQ=255)                                                    cTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGa                                                                                       <  4:202667/149‑1 (MQ=255)                                                      ggATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGt                                                                                     >  6:158812/1‑149 (MQ=255)                                                       gATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTg                                                                                    <  1:550/149‑1 (MQ=255)                                                                aGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTgg                                                                           >  8:101861/1‑149 (MQ=255)                                                                aGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTgg                                                                           >  1:126038/1‑149 (MQ=255)                                                                aGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCATTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTgg                                                                           >  7:156314/1‑149 (MQ=255)                                                                 gCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGc                                                                          <  5:158812/149‑1 (MQ=255)                                                                  cGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGc                                                                          >  3:58999/1‑148 (MQ=255)                                                                      cAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTc                                                                     <  7:101861/149‑1 (MQ=255)                                                                         agcagcGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAg                                                                                 >  7:195653/1‑134 (MQ=255)                                                                         agcagcGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAg                                                                                 <  8:195653/134‑1 (MQ=255)                                                                          gcagcGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTc                                                                 <  4:58999/149‑1 (MQ=255)                                                                              cGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTTCCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGaa                                                             <  5:177563/149‑1 (MQ=255)                                                                              cGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAg                                                               >  5:74626/1‑147 (MQ=255)                                                                              cGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAg                                                               >  7:92849/1‑147 (MQ=255)                                                                              cGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGCCCTTTCAATGACGGCGGCGTTGTTCAACGCCGAACGTGTACCTGTATTGTTTTCACGCCGAGGAATAAAATACCAAAAACGAGTGAAGGGTTTGACACTCGCTCAg                                                               >  1:108082/1‑148 (MQ=255)                                                                                   gCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCa                                                         <  6:51925/148‑1 (MQ=255)                                                                                   gCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCa                                                         <  3:200138/148‑1 (MQ=255)                                                                                   gCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAg                                                        <  6:74626/149‑1 (MQ=255)                                                                                       gCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCAGAAGGTGTACCTGTATTGTATTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCAt                                                    >  7:207977/1‑149 (MQ=255)                                                                                         acaGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAg                                                        <  4:50278/143‑1 (MQ=255)                                                                                         acaGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAg                                                        >  3:50278/1‑143 (MQ=255)                                                                                             tttCCAGGAAACGCAGACCGCGGATGACCTTTCACTGACGGCGGCGTTGTTAAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCaaa                                              >  5:327846/1‑149 (MQ=255)                                                                                               tCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAAcc                                            >  6:157821/1‑149 (MQ=255)                                                                                                ccAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAAcc                                            >  3:229365/1‑148 (MQ=255)                                                                                                  aGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGAt                                         >  7:110048/1‑149 (MQ=255)                                                                                                  aGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGAt                                         >  3:8645/1‑149 (MQ=255)                                                                                                    gAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTc                                                                                                                   <  8:229460/74‑1 (MQ=255)                                                                                                      aaCGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATgcct                                     <  4:229365/149‑1 (MQ=255)                                                                                                         gCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATgcctggc                                  <  7:188576/149‑1 (MQ=255)                                                                                                            gACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATgcctggcctg                               >  1:306582/1‑149 (MQ=255)                                                                                                             aCCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGt                              >  7:43793/1‑149 (MQ=255)                                                                                                             aCCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGt                              >  4:240267/1‑149 (MQ=255)                                                                                                              ccGCGGATGGCCTTTCACTGAGTGCGGCGTTGTTCATGGCCGAATGTTTACCTGTATTGTTTTCAAGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGGCACTCTGTCAGAAGCCAGGCATCGCAAAGCGATGCCTGGCCTGtt                             <  2:108082/149‑1 (MQ=255)                                                                                                              ccGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGtt                             <  8:92849/149‑1 (MQ=255)                                                                                                                         cTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGtttt                           <  8:176846/140‑1 (MQ=255)                                                                                                                         cTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGtttt                           >  7:176846/1‑140 (MQ=255)                                                                                                                         cTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTgg                  >  5:78381/1‑149 (MQ=255)                                                                                                                              aCTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGAttc                                       >  4:189883/1‑121 (MQ=255)                                                                                                                               cTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAAt            <  6:327846/149‑1 (MQ=255)                                                                                                                                tGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGtttt                           <  5:238039/133‑1 (MQ=255)                                                                                                                                tGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGtttt                           >  6:238039/1‑133 (MQ=255)                                                                                                                                tGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATg           >  3:88084/1‑149 (MQ=255)                                                                                                                                 accttcggcgTTGAACAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCt                    >  2:234335/6‑139 (MQ=255)                                                                                                                                 accttcggcgTTGAACAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCt                    <  1:234335/134‑1 (MQ=255)                                                                                                                                  aCGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATgg          <  5:157821/148‑1 (MQ=255)                                                                                                                                  aCGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATgg          <  8:110048/148‑1 (MQ=255)                                                                                                                                  aCGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATgg          <  2:145592/148‑1 (MQ=255)                                                                                                                                   cggcggCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGggt        >  8:165395/1‑149 (MQ=255)                                                                                                                                    ttcggcgTTGAACAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGggtg       >  2:252002/3‑149 (MQ=255)                                                                                                                                      cggcgTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGggtggt     >  4:311796/1‑149 (MQ=255)                                                                                                                                         cgTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAg                                                        >  5:107824/1‑95 (MQ=255)                                                                                                                                         cgTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAg                                                        <  6:107824/95‑1 (MQ=255)                                                                                                                                         cgTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGGGTGGTCGt  <  2:306582/149‑1 (MQ=255)                                                                                                                                             gttCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAAc                                             >  2:270107/1‑102 (MQ=255)                                                                                                                                             gttCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAAc                                             <  1:270107/102‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                 GGCTCTGTCACTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGT‑AATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGGGTGGTCGT  >  NC_002947/3209536‑3209820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑