Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,212,977 T→A 9.4% M15K (ATG→AAG)  mexC → multidrug RND transporter membrane fusion protein MexC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,212,9770TA 9.4% 150.6 / 5.7 53M15K (ATG→AAG) mexCmultidrug RND transporter membrane fusion protein MexC
Reads supporting (aligned to +/- strand):  ref base T (27/21);  new base A (2/3);  total (29/24)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

CTGAAGGGGTCATTAAGAGTCTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCTGAACTGCCGGGCCGGGTCGAGCCGATGC  >  NC_002947/3212830‑3213117
                                                                                                                                                   |                                                                                                                                            
cTGAAGGGGTCATTAAGAGTCTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATg                                                                                                                                             >  3:1857/1‑149 (MQ=255)
cTGAAGGGGTCATTAAGAGTCTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATg                                                                                                                                             >  3:263992/1‑149 (MQ=255)
     ggggTCATTAAGAGTCTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGAt                                                                                                                                        >  1:66255/1‑149 (MQ=255)
         tCATTAAGAGTCTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCTGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCgac                                                                                                                                    >  4:52989/1‑147 (MQ=255)
               atattCTTTTATTATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCAAGGCGATCGCCATGGc                                                                                                                               <  5:276657/144‑1 (MQ=255)
               agagTCTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCAAGGCGATCGCCATGGcc                                                                                                                              <  4:57761/149‑1 (MQ=255)
               agagTCTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCAAGGCGATCGCCATGGc                                                                                                                               >  3:57761/1‑148 (MQ=255)
               agagTCTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGAAAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGcc                                                                                                                              >  1:119354/1‑149 (MQ=255)
                gagTCTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCg                                                                                                                             >  6:84785/1‑149 (MQ=255)
                 agTCTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCgg                                                                                                                            <  5:84785/149‑1 (MQ=255)
                 agTCTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCgg                                                                                                                            >  7:219833/1‑149 (MQ=255)
                    cTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCAAGGCGATCGCCATGGccaat                                                                                                                           >  4:155385/1‑144 (MQ=255)
                    cTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCAAGGCGATCGCCATGGccaat                                                                                                                           <  3:155385/147‑4 (MQ=255)
                     ttttATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCAGCCCCGTTTTCAATCAAGGTTAGTGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGAGCATTGGACATGGCGATCGCCTTGGCCGGgtgt                                                                                                                        >  3:231508/1‑149 (MQ=255)
                       ttATGATTTTAATGAGACAATATTGACGCATGTGGGGTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGCTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGtgtg                                                                                                                       <  7:146913/148‑1 (MQ=255)
                        tATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGa                                                                                                                      <  2:119354/148‑1 (MQ=255)
                         aTGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAg                                                                                                                     <  2:66255/148‑1 (MQ=255)
                                   tGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCgcagca                                                                                                            >  4:82170/1‑147 (MQ=255)
                                      gaCAATATTGACTCATGTGGTTTTGAAAAGTACCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCagcagagca                                                                                                       <  4:231508/149‑1 (MQ=255)
                                      gaCAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCagcagagca                                                                                                       <  4:1857/149‑1 (MQ=255)
                                      gaCAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCagcagagca                                                                                                       <  8:219833/149‑1 (MQ=255)
                                      gaCAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCagcagag                                                                                                         >  5:246748/1‑147 (MQ=255)
                                                aCTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCg                                                                                                                             >  3:306935/1‑117 (MQ=255)
                                                aCTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCg                                                                                                                             <  4:306935/117‑1 (MQ=255)
                                                aCTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCa                                                                                             <  6:246748/149‑1 (MQ=255)
                                                       tgGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATg                                                                                      >  6:33335/1‑149 (MQ=255)
                                                          gTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGa                                                                                   <  6:244006/149‑1 (MQ=255)
                                                           tttGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCagcaga                                                                                                          >  2:71252/1‑125 (MQ=255)
                                                           tttGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAg                                                                                  >  7:224688/1‑149 (MQ=255)
                                                           tttGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGAGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCagcaga                                                                                                          <  1:71252/125‑1 (MQ=255)
                                                               aaaaGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATAGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTAc                                                                              >  1:233986/1‑149 (MQ=255)
                                                                      gCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTg                                                                        <  4:263992/148‑1 (MQ=255)
                                                                      gCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTg                                                                        <  8:111748/148‑1 (MQ=255)
                                                                      gCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTg                                                                        >  7:149449/1‑148 (MQ=255)
                                                                            gCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCg                                                                 >  2:259557/1‑149 (MQ=255)
                                                                             ccccGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGt                                                                <  2:233986/149‑1 (MQ=255)
                                                                                       aatcaaGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGgc                                                       <  5:33335/148‑1 (MQ=255)
                                                                                        atcaaGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGgcgc                                                     >  5:150591/1‑149 (MQ=255)
                                                                                           aaGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCg                                                  <  6:150591/149‑1 (MQ=255)
                                                                                                    gtgtgtGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCg                                         >  7:56424/1‑149 (MQ=255)
                                                                                                         tgATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTg                                    >  5:196048/1‑149 (MQ=255)
                                                                                                         tgATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTg                                    >  4:43435/1‑149 (MQ=255)
                                                                                                           aTGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTgg                                   <  3:43435/148‑1 (MQ=255)
                                                                                                                  aGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCTGaa                           >  7:280132/1‑149 (MQ=255)
                                                                                                                      aGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATc                                                                  >  5:159677/1‑106 (MQ=255)
                                                                                                                      aGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATc                                                                  <  6:159677/106‑1 (MQ=255)
                                                                                                                      aGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCTGAACTgc                       >  8:273647/1‑149 (MQ=255)
                                                                                                                            gATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCTGAACTgccgggcc                 <  3:52989/149‑1 (MQ=255)
                                                                                                                             ataaccgTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGCCGCCCGAGCAGGTCGCCTTGGCGGCTGAACTgccgggccg                >  6:50280/7‑149 (MQ=255)
                                                                                                                                 gggTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCTGAACTGCCGGGCCGGGTc            >  3:217351/1‑149 (MQ=255)
                                                                                                                                           aTTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCTGAACTGCCGGGCCGGGTCGAGCCGATgc  <  3:82170/149‑1 (MQ=255)
                                                                                                                                            ttGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCt                              >  6:218198/1‑120 (MQ=255)
                                                                                                                                            ttGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCt                              <  5:218198/120‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
CTGAAGGGGTCATTAAGAGTCTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCTGAACTGCCGGGCCGGGTCGAGCCGATGC  >  NC_002947/3212830‑3213117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑