Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,212,988 T→A 9.8% L19M (TTG→ATG)  mexC → multidrug RND transporter membrane fusion protein MexC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,212,9880TA 9.8% 137.3 / 5.8 51L19M (TTG→ATG) mexCmultidrug RND transporter membrane fusion protein MexC
Reads supporting (aligned to +/- strand):  ref base T (25/21);  new base A (2/3);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01

AGAGTCTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCTGAACTGCCGGGCCGGGTCGAGCCGATGCGCGTCGCCGAGGTG  >  NC_002947/3212845‑3213131
                                                                                                                                               |                                                                                                                                               
atattCTTTTATTATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCAAGGCGATCGCCATGGc                                                                                                                                             <  5:276657/144‑1 (MQ=255)
agagTCTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCAAGGCGATCGCCATGGcc                                                                                                                                            <  4:57761/149‑1 (MQ=255)
agagTCTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCAAGGCGATCGCCATGGc                                                                                                                                             >  3:57761/1‑148 (MQ=255)
agagTCTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGAAAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGcc                                                                                                                                            >  1:119354/1‑149 (MQ=255)
 gagTCTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCg                                                                                                                                           >  6:84785/1‑149 (MQ=255)
  agTCTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCgg                                                                                                                                          <  5:84785/149‑1 (MQ=255)
  agTCTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCgg                                                                                                                                          >  7:219833/1‑149 (MQ=255)
     cTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCAAGGCGATCGCCATGGccaat                                                                                                                                         >  4:155385/1‑144 (MQ=255)
     cTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCAAGGCGATCGCCATGGccaat                                                                                                                                         <  3:155385/147‑4 (MQ=255)
      ttttATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCAGCCCCGTTTTCAATCAAGGTTAGTGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGAGCATTGGACATGGCGATCGCCTTGGCCGGgtgt                                                                                                                                      >  3:231508/1‑149 (MQ=255)
        ttATGATTTTAATGAGACAATATTGACGCATGTGGGGTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGCTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGtgtg                                                                                                                                     <  7:146913/148‑1 (MQ=255)
         tATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGa                                                                                                                                    <  2:119354/148‑1 (MQ=255)
          aTGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAg                                                                                                                                   <  2:66255/148‑1 (MQ=255)
                    tGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCgcagca                                                                                                                          >  4:82170/1‑147 (MQ=255)
                       gaCAATATTGACTCATGTGGTTTTGAAAAGTACCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCagcagagca                                                                                                                     <  4:231508/149‑1 (MQ=255)
                       gaCAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCagcagagca                                                                                                                     <  4:1857/149‑1 (MQ=255)
                       gaCAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCagcagagca                                                                                                                     <  8:219833/149‑1 (MQ=255)
                       gaCAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCagcagag                                                                                                                       >  5:246748/1‑147 (MQ=255)
                                 aCTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCg                                                                                                                                           <  4:306935/117‑1 (MQ=255)
                                 aCTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCg                                                                                                                                           >  3:306935/1‑117 (MQ=255)
                                 aCTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCa                                                                                                           <  6:246748/149‑1 (MQ=255)
                                        tgGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATg                                                                                                    >  6:33335/1‑149 (MQ=255)
                                           gTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGa                                                                                                 <  6:244006/149‑1 (MQ=255)
                                            tttGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCagcaga                                                                                                                        >  2:71252/1‑125 (MQ=255)
                                            tttGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAg                                                                                                >  7:224688/1‑149 (MQ=255)
                                            tttGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGAGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCagcaga                                                                                                                        <  1:71252/125‑1 (MQ=255)
                                                aaaaGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATAGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTAc                                                                                            >  1:233986/1‑149 (MQ=255)
                                                       gCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTg                                                                                      <  4:263992/148‑1 (MQ=255)
                                                       gCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTg                                                                                      >  7:149449/1‑148 (MQ=255)
                                                       gCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTg                                                                                      <  8:111748/148‑1 (MQ=255)
                                                             gCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCg                                                                               >  2:259557/1‑149 (MQ=255)
                                                              ccccGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGt                                                                              <  2:233986/149‑1 (MQ=255)
                                                                        aatcaaGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGgc                                                                     <  5:33335/148‑1 (MQ=255)
                                                                         atcaaGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGgcgc                                                                   >  5:150591/1‑149 (MQ=255)
                                                                            aaGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCg                                                                <  6:150591/149‑1 (MQ=255)
                                                                                     gtgtgtGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCg                                                       >  7:56424/1‑149 (MQ=255)
                                                                                          tgATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTg                                                  >  5:196048/1‑149 (MQ=255)
                                                                                          tgATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTg                                                  >  4:43435/1‑149 (MQ=255)
                                                                                            aTGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTgg                                                 <  3:43435/148‑1 (MQ=255)
                                                                                                   aGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCTGaa                                         >  7:280132/1‑149 (MQ=255)
                                                                                                       aGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATc                                                                                <  6:159677/106‑1 (MQ=255)
                                                                                                       aGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATc                                                                                >  5:159677/1‑106 (MQ=255)
                                                                                                       aGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCTGAACTgc                                     >  8:273647/1‑149 (MQ=255)
                                                                                                             gATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCTGAACTgccgggcc                               <  3:52989/149‑1 (MQ=255)
                                                                                                              ataaccgTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGCCGCCCGAGCAGGTCGCCTTGGCGGCTGAACTgccgggccg                              >  6:50280/7‑149 (MQ=255)
                                                                                                                  gggTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCTGAACTGCCGGGCCGGGTc                          >  3:217351/1‑149 (MQ=255)
                                                                                                                            aTTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCTGAACTGCCGGGCCGGGTCGAGCCGATgc                <  3:82170/149‑1 (MQ=255)
                                                                                                                             ttGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCt                                            <  5:218198/120‑1 (MQ=255)
                                                                                                                             ttGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCt                                            >  6:218198/1‑120 (MQ=255)
                                                                                                                                        gATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCTGAACTGCCGGGCCGGGTCGAGCCGATGCGCGTCGCCGAgg    >  5:160879/1‑149 (MQ=255)
                                                                                                                                          tCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCTGAACTGCCGGGCCGGGTCGAGCCGATGCGCGTCGCCGAGGTg  >  2:318971/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                               
AGAGTCTTTTATGATTTTAATGAGACAATATTGACTCATGTGGGTTTGAAAAGTAGCATCCGCCCCGTTTTCAATCAAGGTTCGGGTGTGTGATGGGCAAGACAGGTTCGATAAGGGTGGGCGCATTGGCCATGGCGATCGCCTTGGCCGGGTGTGAGCCCGCAGCAGAGCAGGACGGTGCAGCCAATGCGAGCTACCCGGTGGAAATCGTGACATTGGCGCCCGAGCAGGTCGCCTTGGCGGCTGAACTGCCGGGCCGGGTCGAGCCGATGCGCGTCGCCGAGGTG  >  NC_002947/3212845‑3213131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑