Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,240,644 T→C 6.8% F114L (TTC→CTC)  PP_2838 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,240,6440TC 6.8% 172.7 / 2.5 59F114L (TTC→CTC) PP_2838hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (28/27);  new base C (2/2);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

GCAGCGATCGGCTCGACTACGCCGAAATCGGCGATACTGCGCTGGCGCTGCAGCCGTTGCTGGCCCTCGGCTGGGTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAACCCCTGGCGCTGGCAGCCCGGTCACTGGTCGAATGGTTTCCTGACAGCGATGTGCGCATCCTCCAGTGGTGCCTGCAACCTT  >  NC_002947/3240498‑3240785
                                                                                                                                                  |                                                                                                                                             
gCAGCGATCGGCTCGACTACGCCGAAATCGGCGATACTGCGCTGGCGCTGCAGCCGTTGCTGGCCCTCGGCTGGGTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTc                                                                                                                                             >  3:275409/1‑149 (MQ=255)
 cAGCGATCGGCTCGACTACGCCGAAATCGGCGATACTGCGCTGGCGCTGCAGCCGTTGCTGGCCCTCGGCTGGGTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCtt                                                                                                                                              >  8:57772/1‑147 (MQ=255)
 cAGCGATCGGCTCGACTACGCCGAAATCGGCGATACTGCGCTGGCGCTGCAGCCGTTGCTGGCCCTCGGCTGGGTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCtt                                                                                                                                              <  7:57772/147‑1 (MQ=255)
 cAGCGATCGGCTCGACTACGCCGAAATCGGCGATACTGCGCTGGCGCTGCAGCCGTTGCTGGCCCTCGGCTGGGTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTc                                                                                                                                             >  5:18702/1‑148 (MQ=255)
 cAGCGATCGGCTCGACTACGCCGAAATCGGCGATACTGCGCTGGCGCTGCAGCCGTTGCTGGCCCTCGGCTGGGTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTc                                                                                                                                             <  6:18702/148‑1 (MQ=255)
  aGCGATCGGCTCGACTACGCCGAAATCGGCGATACTGCGCTGGCGCTGCAGGCGTTGCTGGCCCTCGGCTGGGTACGGGAGCCCGAGCAACTTGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCtt                                                                                                                                              <  4:117380/146‑1 (MQ=255)
  aGCGATCGGCTCGACTACGCCGAAATCGGCGATACTGCGCTGGCGCTGCAGCCGTTGCTGGCCCTCGGCTGGGTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCtt                                                                                                                                              >  3:117380/1‑146 (MQ=255)
   gcaaTCGGCTCGACTACGCCGAAATCGGCGATACTGCGCTGGCGCTGCAGCCGTTGCTGGCCCTCGGCTGGGTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGAAAGGAAGAACTGGCCCGCTGCTTCGcc                                                                                                                                          >  5:158277/4‑149 (MQ=255)
      aTCGGCTCGACTACGCCGAAATCGGCGATACTGCGCTGGCGCTGCAGCCGTTGCTGGCCCTCGGCTGGGTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCgg                                                                                                                                       >  3:121922/1‑149 (MQ=255)
           cTCGACTACGCCGAAATCGGCGATACTGCGCTGGCGCTGCAGCCGTTGCTGGCCCTCGGCTGGGTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACt                                                                                                                                  <  7:22341/149‑1 (MQ=255)
              gACTACGCCGAAATCGGCGATACTGCGCTGGCGCTGCAGCCGTTGCTGGCCCTCGGCTGGGTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGaa                                                                                                                               <  6:30374/149‑1 (MQ=255)
                 tACGCCGAAATCGGCGATACTGCGCTGGCGCTGCAGCCGTTGCTGGCCCTCGGCTGGGTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAAccg                                                                                                                            >  8:129300/1‑149 (MQ=255)
                   cGCCGAAATCGGCGATACTGCGCTGGCGCTGCAGCCGTTGCTGGCCCTCGGCTGGGTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAAccgcc                                                                                                                          >  2:225109/1‑149 (MQ=255)
                                       cgctggcgctgCAGCCGTTGCTGGCCCTCGGCTGGGTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAg                                                                                                      >  4:266118/1‑149 (MQ=255)
                                           ggcgctgCAGCCGTTGCTGGCCCTCGGTTGGGTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCgg                                                                                                                                       >  1:112169/1‑112 (MQ=255)
                                           ggcgctgCAGCCGTTGCTGGCCCTCGGTTGGGTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCgg                                                                                                                                       <  2:112169/112‑1 (MQ=255)
                                            gcgctgCAGCCGTTGCTGGCCCTCGGCTGGGTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAgttgtt                                                                                                 <  4:275409/149‑1 (MQ=255)
                                             cgctgCAGCCGTTGCTGGCCCTCGGCTGGGTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCATCTGAACCGCCCGCGCGCGGCCAAGCATGAGttgttg                                                                                                <  1:54200/149‑1 (MQ=255)
                                                      ggTTGCTGGCCCTCGGCTGGGGACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTg                                                                                       <  6:205036/148‑1 (MQ=255)
                                                        ttGCTGGCCCTCGGCTGGGTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGc                                                                                      >  7:192071/1‑148 (MQ=255)
                                                          gCTGGCCCTCGGCTGGGTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGTGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCaa                                                                                    <  1:270927/148‑1 (MQ=255)
                                                          gCTGGCCCTCGGCTGGGTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAAc                                                                                   >  8:283056/1‑149 (MQ=255)
                                                              gCCCTCGGCTGGGTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAACCCCt                                                                               <  6:217329/149‑1 (MQ=255)
                                                              gCCCTCGGCTGGGTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAACCCCt                                                                               <  6:33337/149‑1 (MQ=255)
                                                               cccTCGGCTGGGTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGc                                                                                         <  3:277534/138‑1 (MQ=255)
                                                               cccTCGGCTGGGTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGc                                                                                         >  4:277534/1‑138 (MQ=255)
                                                               cccTCGGCTGGGTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAACCCCTg                                                                              <  1:138945/149‑1 (MQ=255)
                                                                    ggCTTGGTACGGTATCCCGAGCAACTGGACCTTGAGCAGCTGATCGCGTTGCTGCGCAAGGAATAACTTGCCCGCTGCTTCCCCCGGCAACTGAACCGCCCGCGCGAGGCCAAGCATGAGTTTTTGGCACAGCTGCAACCCCTGGCGCt                                                                         <  7:218178/149‑1 (MQ=255)
                                                                    ggCTGGGTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAACCCCTGGCGCt                                                                         <  7:129300/149‑1 (MQ=255)
                                                                     gCTGGGGACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAACCCCTGGCGCTg                                                                        <  8:244933/149‑1 (MQ=255)
                                                                          gTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCaa                                                                                    <  5:74213/132‑1 (MQ=255)
                                                                          gTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCaa                                                                                    >  6:74213/1‑132 (MQ=255)
                                                                          gTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAACCCCTGGCGCTGGCAGc                                                                   >  5:23752/1‑149 (MQ=255)
                                                                              gggAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAACCCCTGGCGCTGGCAGCCCgg                                                               >  4:75727/1‑149 (MQ=255)
                                                                               ggAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAACCCCTGGCGCTGGCAGCCCGGt                                                              >  6:135011/1‑149 (MQ=255)
                                                                               ggAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAACCCCTCGCGCTGGCAGCCCGGt                                                              >  6:279741/1‑149 (MQ=255)
                                                                                gAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAgttgt                                                                                                  >  4:237078/1‑112 (MQ=255)
                                                                                gAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAgttgt                                                                                                  <  3:237078/112‑1 (MQ=255)
                                                                                   cccGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAACCCCTGGCGCTGGCAGCCCGGTCACt                                                          <  4:121922/149‑1 (MQ=255)
                                                                                        gCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAACCCCTGGCGCTGGCAGCCCGGTCACTGGTc                                                      >  2:187209/1‑148 (MQ=255)
                                                                                           aatGGACATTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGACAGCTCCTTCGCCCGGCAACTGAACCGCCCGCGCGCGTCCAAGCAt                                                                                                         >  5:305224/3‑94 (MQ=255)
                                                                                           aCTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCTCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCAt                                                                                                         <  6:305224/94‑1 (MQ=255)
                                                                                            cTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGACAAGCATGAGTTGTTGGCACAGCTGCAACCCCTGGCGCTGGCAGCCCGCTCACTGGTCGAATg                                                 >  1:39808/1‑149 (MQ=255)
                                                                                             tGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAACCCCTGGCGCTGGCAGCCCGGTCACTGGTCGAATgg                                                >  7:95620/1‑149 (MQ=255)
                                                                                              ggACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAACCCCTGGCGCTGGCAGCCCGGTCACTGGTCGAATGGt                                               >  1:126002/1‑149 (MQ=255)
                                                                                                aCCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAACCCCTGGCGCTGGCAGCCCGGTCACTGGTCGAATGGttt                                             <  1:187209/149‑1 (MQ=255)
                                                                                                              ttCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAACCCCTGGCGCTGGCAGCCCGGTCACTGGTCGAATGGTTTCCTGACAGCGAtgt                               >  4:215044/1‑149 (MQ=255)
                                                                                                                cgcgTTGCTGCGCAAGGAAGAACTGGCCCGCTGCCTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAACCCCTGGCGCTGGCAGCCCGGTCACTGGTCGAATGGTTTCCTGACAGCGAtgtg                              >  6:158249/1‑148 (MQ=255)
                                                                                                                cgcgTTGCTGCGCAAGGAAGAACTGGCCCGCTGCCTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAACCCCTGGCGCTGGCAGCCCGGTCACTGGTCGAATGGTTTCCTGACAGCGAtgtg                              >  6:153658/1‑148 (MQ=255)
                                                                                                                cgcgTTGCTGCGCAAGGAAGAACTGGCCCGCTGCCTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAACCCCTGGCGCTGGCAGCCCGGTCACTGGTCGAATGGTTTCCTGACAGCGAtgtg                              <  5:158249/148‑1 (MQ=255)
                                                                                                                cgcgTTGCTGCGCAAGGAAGAACTGGCCCGCTGCCTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAACCCCTGGCGCTGGCAGCCCGGTCACTGGTCGAATGGTTTCCTGACAGCGAtgtg                              <  5:153658/148‑1 (MQ=255)
                                                                                                                 gcgTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAACCCCTGGCGCTGGCAGCCCGGTCACTGGTCGAATGGTTTCCTGACAGCGATGTgcg                            >  1:316168/1‑149 (MQ=255)
                                                                                                                      gctgcGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAACCCCTGGCGCTGGCAGCCCGGTCACTGGTCGAATGGTTTCCTGACAGCGATGTGCGCAtc                        <  6:23752/148‑1 (MQ=255)
                                                                                                                        tgcGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGc                                                                                      <  1:240696/84‑1 (MQ=255)
                                                                                                                        tgcGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGc                                                                                      >  2:240696/1‑84 (MQ=255)
                                                                                                                          cgcAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGATCCGCCCTCGCGCGGCCAAGCATGAGTTGTTGTCACAGCTGCAACCCCTGGCGCTGGCAGCCCGGTCACTGGTAGAATGGTTTCCTGACAGCGATGTGCGCATCCTCCa                   <  6:158277/149‑1 (MQ=255)
                                                                                                                             aaGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAACCCCTGGCGCTGGCAGCCCGGTCACTGGTCGAATGGTTTCCTGACAGCGATGTGCGCATCCTCCAgtg                <  3:266118/149‑1 (MQ=255)
                                                                                                                                   gaaCTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAACCCCTGGCGCTGGCAGCCCGGTCACTGGTCGAAt                                                  >  3:140810/1‑109 (MQ=255)
                                                                                                                                   gaaCTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAACCCCTGGCGCTGGCAGCCCGGTCACTGGTCGAAt                                                  <  4:140810/109‑1 (MQ=255)
                                                                                                                                     aCTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAACCCCTGGCGCTGGCAGCCCGGTCACTGGTCGAATGGTTTCCTGACAGCGATGTGCGCATCCTCCAGTGGTGCCTGc        <  7:283056/149‑1 (MQ=255)
                                                                                                                                          cccGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAACCCCTGGCGCTGGCAGCCCGGTCACTGGTCGAATGGTTTCCTGACAGCGAtgt                               >  7:45875/1‑121 (MQ=255)
                                                                                                                                          cccGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAACCCCTGGCGCTGGCAGCCCGGTCACTGGTCGAATGGGTTCCTGACAGCGAtgt                               <  8:45875/121‑1 (MQ=255)
                                                                                                                                           ccGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAACCCCTGGCGCTGGCAGCCCGGTCACTGGTCGAATGGTTTCCTGACAGCGATGTGCGCATCCTCCAGTGGTGCCTGCAACCtt  >  3:154062/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                             
GCAGCGATCGGCTCGACTACGCCGAAATCGGCGATACTGCGCTGGCGCTGCAGCCGTTGCTGGCCCTCGGCTGGGTACGGGAGCCCGAGCAACTGGACCTTGAGCAGCTGTTCGCGTTGCTGCGCAAGGAAGAACTGGCCCGCTGCTTCGCCCGGCAACTGAACCGCCCGCGCGCGGCCAAGCATGAGTTGTTGGCACAGCTGCAACCCCTGGCGCTGGCAGCCCGGTCACTGGTCGAATGGTTTCCTGACAGCGATGTGCGCATCCTCCAGTGGTGCCTGCAACCTT  >  NC_002947/3240498‑3240785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑