Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 308,739 A→G 9.4% intergenic (+1754/‑115) hslO → / → PP_0254 molecular chaperone HslO/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947308,7390AG 9.4% 145.9 / 4.3 53intergenic (+1754/‑115)hslO/PP_0254molecular chaperone HslO/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (23/25);  new base G (2/3);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.94e-01

CAAAGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCAC  >  NC_002947/308597‑308876
                                                                                                                                              |                                                                                                                                         
cAAAGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGggcg                                                                                                                                     >  6:333269/1‑149 (MQ=255)
  aaGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGggcggc                                                                                                                                   >  4:15029/1‑149 (MQ=255)
        ccTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCTGGGGGCGGCtttctc                                                                                                                             <  2:6158/149‑4 (MQ=255)
         cTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTtg                                                                                                                            >  2:274737/1‑149 (MQ=255)
          tACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCGGTAAGCCGAGCTCAG‑CACAACGAAAAAGCCGCCCCTGGGGGCGGCtttctcgtt                                                                                                                          <  8:272413/149‑7 (MQ=255)
            cgacgcGGCTGCCAAAGGTCTGGCAAATCGGTTCATCGAAAACTTCAAGAATTTCGAAGTTTCCGACGCCATCAATGCCGCTGGCCCGCAGCTTTAAGCCGAGCTCAG‑AACAACTAAAAAGCCGCACCTGGGGGCGGCtttctcgtt                                                                                                                          <  8:276665/147‑7 (MQ=255)
            cgacgaGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTgg                                                                                                                         <  1:274737/149‑1 (MQ=255)
            cgacgaGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTgg                                                                                                                         <  8:238621/149‑1 (MQ=255)
                  ggCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAgg                                                                                                                   <  2:272873/149‑1 (MQ=255)
                   gCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAggg                                                                                                                  >  2:139823/1‑149 (MQ=255)
                    cTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGc                                                                                                                 <  2:87790/149‑1 (MQ=255)
                         aaaGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGcc                                                                                                            >  4:30862/1‑149 (MQ=255)
                                  gCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGaa                                                                                                      <  5:333269/146‑1 (MQ=255)
                                  gCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAg                                                                                                     <  3:30862/147‑1 (MQ=255)
                                       gCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTgg                                                                                                                         >  7:99816/1‑122 (MQ=255)
                                       gCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTgg                                                                                                                         <  8:99816/122‑1 (MQ=255)
                                          gTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCAt                                                                                           >  4:32489/1‑149 (MQ=255)
                                               tCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAgg                                                                                        >  4:37651/1‑147 (MQ=255)
                                                cGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAg                                                                                     >  7:41363/1‑149 (MQ=255)
                                                       ttCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGt                                                                              >  6:204945/1‑149 (MQ=255)
                                                       ttCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGt                                                                              >  5:248165/1‑149 (MQ=255)
                                                          aagaagTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTg                                                                           <  5:320451/149‑1 (MQ=255)
                                                          aagaagTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGG‑‑GGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTgg                                                                          <  3:162434/148‑1 (MQ=255)
                                                                ttCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGttttt                                                                     <  1:139823/149‑1 (MQ=255)
                                                                    aaGTTTCCGACGCCATCAAGGCCGCTGGCTCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAg                                                                                     <  6:216294/129‑1 (MQ=255)
                                                                    aaGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGt                                                                              <  8:60682/136‑1 (MQ=255)
                                                                    aaGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGt                                                                 >  1:125306/1‑149 (MQ=255)
                                                                    aaGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCAGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGt                                                                              >  7:60682/1‑136 (MQ=255)
                                                                    aaGTTTCCGACGCCATCAAGGCCGCTGGCACGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAg                                                                                     >  5:216294/1‑129 (MQ=255)
                                                                      gTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGttt                                                               <  3:15029/149‑1 (MQ=255)
                                                                            gACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGACTCGTTTTTCTGTTTCACTGt                                                         >  2:118364/1‑149 (MQ=255)
                                                                             aCGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCt                                                                   >  4:84955/1‑138 (MQ=255)
                                                                             aCGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCt                                                                   <  3:84955/138‑1 (MQ=255)
                                                                                     aaGGCCGCTGGCCCGCATCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGTTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAATTCTGGTTTTTCTGTTTCACTGTCAGCATcc                                                 <  6:68190/148‑1 (MQ=255)
                                                                                        gCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTg                                             <  6:248165/149‑1 (MQ=255)
                                                                                         ccGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGc                                            <  5:204945/149‑1 (MQ=255)
                                                                                             tGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCg                                           >  2:91721/1‑146 (MQ=255)
                                                                                               gCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCa                                                             >  4:109558/1‑126 (MQ=255)
                                                                                                   gCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTg                                             <  5:283063/138‑1 (MQ=255)
                                                                                                   gCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTg                                             >  6:283063/1‑138 (MQ=255)
                                                                                                   gCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGCTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGGCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAaga                                  <  6:154537/149‑1 (MQ=255)
                                                                                                        tGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGagccag                             >  1:304324/1‑149 (MQ=255)
                                                                                                                gAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGa                     <  8:41363/149‑1 (MQ=255)
                                                                                                                      aGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAg               <  3:37651/149‑1 (MQ=255)
                                                                                                                           ccacaaaaaaGCCGCCCCTGGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCt          >  3:87883/8‑149 (MQ=255)
                                                                                                                                aaaaaGCCGCCACTGGGTGCGGCTTTTTAATGGCATAGCGCATGCCCGCGAAGGGGCACGCATAGGAAGAGAAAGTCTGGATTATCTTTTTAACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGAACACCGAAGCCCCTCcgcg     >  6:158116/3‑149 (MQ=255)
                                                                                                                                  aaaGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGagccagc                            <  2:153586/124‑1 (MQ=255)
                                                                                                                                  aaaGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGagccagc                            >  1:153586/1‑124 (MQ=255)
                                                                                                                                   aaGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCAc  <  2:304324/149‑1 (MQ=255)
                                                                                                                                     gccgccCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAagag                                 <  6:149050/116‑1 (MQ=255)
                                                                                                                                     gccgccCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAagag                                 >  5:149050/1‑116 (MQ=255)
                                                                                                                                      ccgccCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAgc      <  1:77364/142‑1 (MQ=255)
                                                                                                                                      ccgccCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAgc      >  2:77364/1‑142 (MQ=255)
                                                                                                                                              |                                                                                                                                         
CAAAGCGGCCTACGACGAGGCTGCCAAAGGTCTGGCCAAGCTGTTCATCGAAAACTTCAAGAAGTTCGAAGTTTCCGACGCCATCAAGGCCGCTGGCCCGCAGCTGTAAGCCGAGCTCAGCCGCAACGAGAAAGCCGCCCCCAGGGGCGGCTTTTTTGTGGGTTAGGGCTTGCCCGCGAAGGGGCCCGCATAGGCAGCGAAAGTCTGGTTTTTCTGTTTCACTGTCAGCATCCATTGCGAAGACAAGAGCCAGCCAGATGACCGAAGCCCCTCAGCGCAC  >  NC_002947/308597‑308876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑