Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,255,843	C→T	9.7%	intergenic (‑106/+30)	PP_2852 ← / ← PP_2853	sulfatase domain‑containing protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,255,843	0	C	T	9.7%	168.8 / 7.2	62	intergenic (‑106/+30)	PP_2852/PP_2853	sulfatase domain‑containing protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (22/34);  new base T (3/3);  total (25/37)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-01

GGCAAGCCGGGTGCTTGGGTGGTCGGACAGGTTCTTCACAAAAACTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGTTGTCGGTGGCGGCGAAGGTCAGGCCAAT  >  NC_002947/3255699‑3255989                                                                                                                                                  |                                                                                                                                                   ggCAAGCCGGGTGCTTGGGTGGTCGGACAGGTTCTTCACAAAAACTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTc                                                                                                                                                >  5:139093/1‑149 (MQ=255)  gCAAGCCGGGTGCTTGGGTGGTCGGACAGGTTCTTCACAAAAACTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCg                                                                                                                                               >  1:255626/1‑149 (MQ=255)       ccGGGTGCTTGGGTGGTCGGACAGGTTCTTCACAAAAGCTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTGACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAg                                                                                                                                          >  8:180064/1‑149 (MQ=255)          ggTGCTTGGGTGGTCGGACAGGTTCTTCACAAAAACTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGcac                                                                                                                                            >  7:239945/1‑144 (MQ=255)          ggTGCTTGGGTGGTCGGACAGGTTCTTCACAAAAACTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGcac                                                                                                                                            <  8:239945/144‑1 (MQ=255)            tGCTTGGGTGGTCGGACAGGTTCTTCACAAAAACTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCg                                                                                                                                     <  4:108655/149‑1 (MQ=255)               ttGGGTGGTCGGACAGGTTCTTCACAAAAACTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGcc                                                                                                                                  <  6:139093/149‑1 (MQ=255)                 gggTGGTCGGACAGGTTCTTCACAAAAACTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCTGTGCGAACGGGCCCGGct                                                                                                                                  <  3:46433/147‑2 (MQ=255)                         ggACAGGTTCTTCACAAAAACTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCACGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAAc                                                                                                                         <  2:41891/148‑1 (MQ=255)                          gACAGGTTCTTAACAAAAAATTACCTTTTGCCATTAAATCGTCATGCATCTGTGGTTGA‑TGATATAAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTAGCCCAGGCCCCGCCGATGGCAACTa                                                                                                                       >  2:115292/1‑149 (MQ=255)                                ttcttcACAAAAACTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAg                                                                                                                   >  6:143305/1‑147 (MQ=255)                                  cttcACAAAAACTTACCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCTGCCAGTTACAAGGCTTGTAACAAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAg                                                                                                                <  7:126690/148‑1 (MQ=255)                                   ttcACAAAAACTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGt                                                                                                               >  4:64220/1‑148 (MQ=255)                                        aaaaaCTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGAGTGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGaa                                                                                                                 >  1:229115/1‑142 (MQ=255)                                        aaaaaCTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTAt                                                                                                                      >  4:309128/1‑136 (MQ=255)                                        aaaaaCTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTAt                                                                                                                      <  3:309128/136‑1 (MQ=255)                                        aaaaaCTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGaa                                                                                                                 <  2:229115/141‑1 (MQ=255)                                        aaaaaCTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAg                                                                                                         <  3:248440/149‑1 (MQ=255)                                                 ccTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAAttt                                                                                                  >  1:48823/1‑147 (MQ=255)                                                       gCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCt                                                                                            >  8:214365/1‑147 (MQ=255)                                                       gCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCt                                                                                            <  7:214365/147‑1 (MQ=255)                                                          aTTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGcc                                                                                                                                       <  1:197277/101‑1 (MQ=255)                                                          aTTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGcc                                                                                                                                       >  2:197277/1‑101 (MQ=255)                                                             aaaTCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGa                                                                                                      >  6:283027/1‑131 (MQ=255)                                                             aaaTCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGa                                                                                                      <  5:283027/131‑1 (MQ=255)                                                              aaTCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGaaaa                                                                                   <  2:48823/149‑1 (MQ=255)                                                                tCGTCATGCATCTGTGCTTGA‑TGAAATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACAAAAGCCGGGGCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGt                                                                                 <  6:67145/149‑1 (MQ=255)                                                                  gTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAATGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGt                                                                                 >  5:205972/1‑147 (MQ=255)                                                                   tCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAg                                                                              <  2:92662/149‑1 (MQ=255)                                                                   tCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAg                                                                              <  7:44853/149‑1 (MQ=255)                                                                              ttcTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATc                                                                    <  8:234401/146‑1 (MQ=255)                                                                              tgCTTGA‑TGATCTCCGCCGAGGTCAGGGATGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAACGTAATTTCAGTCTGAAAGAAAAGTCAGGTGCGTCATc                                                                    <  7:214432/148‑1 (MQ=255)                                                                              tgCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTACGTCATc                                                                    <  2:255626/148‑1 (MQ=255)                                                                              tgCTTGA‑TGATATAAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATc                                                                    <  4:291669/148‑1 (MQ=255)                                                                                cTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAAttt                                                                                                  >  3:201027/1‑116 (MQ=255)                                                                                cTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAAttt                                                                                                  <  4:201027/116‑1 (MQ=255)                                                                                           tCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAAt                                                        <  5:143305/148‑1 (MQ=255)                                                                                            cAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATg                                                       >  5:318260/1‑148 (MQ=255)                                                                                               gCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACa                                                   <  1:115292/149‑1 (MQ=255)                                                                                                    ggcagggAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTg                                              <  6:318260/149‑1 (MQ=255)                                                                                                     gcagggAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTgg                                             <  3:64220/149‑1 (MQ=255)                                                                                                     gcagggAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTgg                                             >  8:144234/1‑149 (MQ=255)                                                                                                      cagggAAGGCTTCCAGTTTCTAGGCTTGTAACCAAAGCCGGGCCCGTTCGAAAAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCTTGCAGACCAATGTACAGGTTggg                                            <  6:205972/149‑1 (MQ=255)                                                                                                                  ccAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAg                                <  4:62652/149‑1 (MQ=255)                                                                                                                   cAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAgg                               >  4:271039/1‑149 (MQ=255)                                                                                                                    aGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTGAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGt                              <  3:276919/149‑1 (MQ=255)                                                                                                                    aGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGt                              <  6:239962/149‑1 (MQ=255)                                                                                                                         cAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGTTGTCg                         <  6:95361/149‑1 (MQ=255)                                                                                                                           aGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAAGTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGAGAGGTTGGGCACGATGGTCAGGTTGTCGGt                       >  2:202229/1‑149 (MQ=255)                                                                                                                            ggCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGAt                                      >  7:281647/1‑133 (MQ=255)                                                                                                                            ggCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGAt                                      <  8:281647/133‑1 (MQ=255)                                                                                                                            ggCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGTTGTCGGTg                      <  7:180064/149‑1 (MQ=255)                                                                                                                             gCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGTTGTCGGTgg                     <  7:144234/149‑1 (MQ=255)                                                                                                                                     ccAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGTTGTCgg                        <  7:190078/138‑1 (MQ=255)                                                                                                                                     ccAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGTTGTCgg                        >  8:190078/1‑138 (MQ=255)                                                                                                                                     ccAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGTTGTCGGTGGCGGCGAAg             >  6:83281/1‑149 (MQ=255)                                                                                                                                     ccAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGTTGTCGGTGGCGGCGAAg             <  3:271039/149‑1 (MQ=255)                                                                                                                                     catcggCCGGGCCTGTGCGAACGGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAAt                                                        <  4:81821/101‑1 (MQ=38)                                                                                                                                     catcggCCGGGCCTGTGCGAACGGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAAt                                                        >  3:81821/6‑106 (MQ=38)                                                                                                                                            cGGGCCTGTGCGAACGGGCCCGGCTGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGTTGTCGGTggcgg                  >  4:195130/1‑137 (MQ=255)                                                                                                                                            cGGGCCTGTGCGAACGGGCCCGGCTGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGTTGTCGGTggcgg                  <  3:195130/137‑1 (MQ=255)                                                                                                                                            cGGGCCTGTGCGAACGGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGTTGTCGGTGGCGGCGAAGGTCAGGc      >  5:67253/1‑149 (MQ=255)                                                                                                                                                cccGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGTTGTCGGTGGCGGCGAAGGTCAGGCCAat  <  1:202229/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                   GGCAAGCCGGGTGCTTGGGTGGTCGGACAGGTTCTTCACAAAAACTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGTTGTCGGTGGCGGCGAAGGTCAGGCCAAT  >  NC_002947/3255699‑3255989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑