Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,255,846	T→G	9.4%	intergenic (‑109/+27)	PP_2852 ← / ← PP_2853	sulfatase domain‑containing protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,255,846	0	T	G	9.4%	179.7 / 10.5	64	intergenic (‑109/+27)	PP_2852/PP_2853	sulfatase domain‑containing protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (23/35);  new base G (3/3);  total (26/38)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.24e-01

GGCAAGCCGGGTGCTTGGGTGGTCGGACAGGTTCTTCACAAAAACTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGTTGTCGGTGGCGGCGAAGGTCAGGCCAATATT  >  NC_002947/3255699‑3255992                                                                                                                                                     |                                                                                                                                                   ggCAAGCCGGGTGCTTGGGTGGTCGGACAGGTTCTTCACAAAAACTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTc                                                                                                                                                   >  5:139093/1‑149 (MQ=255)  gCAAGCCGGGTGCTTGGGTGGTCGGACAGGTTCTTCACAAAAACTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCg                                                                                                                                                  >  1:255626/1‑149 (MQ=255)       ccGGGTGCTTGGGTGGTCGGACAGGTTCTTCACAAAAGCTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTGACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAg                                                                                                                                             >  8:180064/1‑149 (MQ=255)          ggTGCTTGGGTGGTCGGACAGGTTCTTCACAAAAACTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGcac                                                                                                                                               <  8:239945/144‑1 (MQ=255)          ggTGCTTGGGTGGTCGGACAGGTTCTTCACAAAAACTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGcac                                                                                                                                               >  7:239945/1‑144 (MQ=255)            tGCTTGGGTGGTCGGACAGGTTCTTCACAAAAACTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCg                                                                                                                                        <  4:108655/149‑1 (MQ=255)               ttGGGTGGTCGGACAGGTTCTTCACAAAAACTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGcc                                                                                                                                     <  6:139093/149‑1 (MQ=255)                 gggTGGTCGGACAGGTTCTTCACAAAAACTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCTGTGCGAACGGGCCCGGct                                                                                                                                     <  3:46433/147‑2 (MQ=255)                         ggACAGGTTCTTCACAAAAACTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCACGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAAc                                                                                                                            <  2:41891/148‑1 (MQ=255)                          gACAGGTTCTTAACAAAAAATTACCTTTTGCCATTAAATCGTCATGCATCTGTGGTTGA‑TGATATAAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTAGCCCAGGCCCCGCCGATGGCAACTa                                                                                                                          >  2:115292/1‑149 (MQ=255)                                ttcttcACAAAAACTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAg                                                                                                                      >  6:143305/1‑147 (MQ=255)                                  cttcACAAAAACTTACCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCTGCCAGTTACAAGGCTTGTAACAAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAg                                                                                                                   <  7:126690/148‑1 (MQ=255)                                   ttcACAAAAACTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGt                                                                                                                  >  4:64220/1‑148 (MQ=255)                                        aaaaaCTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGAGTGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGaa                                                                                                                    >  1:229115/1‑142 (MQ=255)                                        aaaaaCTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTAt                                                                                                                         >  4:309128/1‑136 (MQ=255)                                        aaaaaCTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTAt                                                                                                                         <  3:309128/136‑1 (MQ=255)                                        aaaaaCTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGaa                                                                                                                    <  2:229115/141‑1 (MQ=255)                                        aaaaaCTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAg                                                                                                            <  3:248440/149‑1 (MQ=255)                                                 ccTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAAttt                                                                                                     >  1:48823/1‑147 (MQ=255)                                                       gCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCt                                                                                               <  7:214365/147‑1 (MQ=255)                                                       gCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCt                                                                                               >  8:214365/1‑147 (MQ=255)                                                          aTTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGcc                                                                                                                                          <  1:197277/101‑1 (MQ=255)                                                          aTTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGcc                                                                                                                                          >  2:197277/1‑101 (MQ=255)                                                             aaaTCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGa                                                                                                         >  6:283027/1‑131 (MQ=255)                                                             aaaTCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGa                                                                                                         <  5:283027/131‑1 (MQ=255)                                                              aaTCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGaaaa                                                                                      <  2:48823/149‑1 (MQ=255)                                                                tCGTCATGCATCTGTGCTTGA‑TGAAATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACAAAAGCCGGGGCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGt                                                                                    <  6:67145/149‑1 (MQ=255)                                                                  gTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAATGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGt                                                                                    >  5:205972/1‑147 (MQ=255)                                                                   tCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAg                                                                                 <  2:92662/149‑1 (MQ=255)                                                                   tCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAg                                                                                 <  7:44853/149‑1 (MQ=255)                                                                              ttcTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATc                                                                       <  8:234401/146‑1 (MQ=255)                                                                              tgCTTGA‑TGATCTCCGCCGAGGTCAGGGATGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAACGTAATTTCAGTCTGAAAGAAAAGTCAGGTGCGTCATc                                                                       <  7:214432/148‑1 (MQ=255)                                                                              tgCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTACGTCATc                                                                       <  2:255626/148‑1 (MQ=255)                                                                              tgCTTGA‑TGATATAAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATc                                                                       <  4:291669/148‑1 (MQ=255)                                                                                cTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAAttt                                                                                                     >  3:201027/1‑116 (MQ=255)                                                                                cTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAAttt                                                                                                     <  4:201027/116‑1 (MQ=255)                                                                                           tCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAAt                                                           <  5:143305/148‑1 (MQ=255)                                                                                            cAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATg                                                          >  5:318260/1‑148 (MQ=255)                                                                                               gCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACa                                                      <  1:115292/149‑1 (MQ=255)                                                                                                    ggcagggAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTg                                                 <  6:318260/149‑1 (MQ=255)                                                                                                     gcagggAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTgg                                                <  3:64220/149‑1 (MQ=255)                                                                                                     gcagggAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTgg                                                >  8:144234/1‑149 (MQ=255)                                                                                                      cagggAAGGCTTCCAGTTTCTAGGCTTGTAACCAAAGCCGGGCCCGTTCGAAAAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCTTGCAGACCAATGTACAGGTTggg                                               <  6:205972/149‑1 (MQ=255)                                                                                                                  ccAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAg                                   <  4:62652/149‑1 (MQ=255)                                                                                                                   cAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAgg                                  >  4:271039/1‑149 (MQ=255)                                                                                                                    aGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTGAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGt                                 <  3:276919/149‑1 (MQ=255)                                                                                                                    aGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGt                                 <  6:239962/149‑1 (MQ=255)                                                                                                                         cAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGTTGTCg                            <  6:95361/149‑1 (MQ=255)                                                                                                                           aGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAAGTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGAGAGGTTGGGCACGATGGTCAGGTTGTCGGt                          >  2:202229/1‑149 (MQ=255)                                                                                                                            ggCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGAt                                         <  8:281647/133‑1 (MQ=255)                                                                                                                            ggCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGAt                                         >  7:281647/1‑133 (MQ=255)                                                                                                                            ggCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGTTGTCGGTg                         <  7:180064/149‑1 (MQ=255)                                                                                                                             gCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGTTGTCGGTgg                        <  7:144234/149‑1 (MQ=255)                                                                                                                                     ccAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGTTGTCgg                           <  7:190078/138‑1 (MQ=255)                                                                                                                                     ccAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGTTGTCgg                           >  8:190078/1‑138 (MQ=255)                                                                                                                                     ccAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGTTGTCGGTGGCGGCGAAg                <  3:271039/149‑1 (MQ=255)                                                                                                                                     ccAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGTTGTCGGTGGCGGCGAAg                >  6:83281/1‑149 (MQ=255)                                                                                                                                     catcggCCGGGCCTGTGCGAACGGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAAt                                                           <  4:81821/101‑1 (MQ=38)                                                                                                                                     catcggCCGGGCCTGTGCGAACGGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAAt                                                           >  3:81821/6‑106 (MQ=38)                                                                                                                                            cGGGCCTGTGCGAACGGGCCCGGCTGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGTTGTCGGTggcgg                     <  3:195130/137‑1 (MQ=255)                                                                                                                                            cGGGCCTGTGCGAACGGGCCCGGCTGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGTTGTCGGTggcgg                     >  4:195130/1‑137 (MQ=255)                                                                                                                                            cGGGCCTGTGCGAACGGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGTTGTCGGTGGCGGCGAAGGTCAGGc         >  5:67253/1‑149 (MQ=255)                                                                                                                                                cccGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGTTGTCGGTGGCGGCGAAGGTCAGGCCAat     <  1:202229/149‑1 (MQ=255)                                                                                                                                                   gTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGTTGTCGGTGGCGGCGAAGGTCAGGCCAATAtt  >  6:17617/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GGCAAGCCGGGTGCTTGGGTGGTCGGACAGGTTCTTCACAAAAACTTCCCTTTTGCCATTAAATCGTCATGCATCTGTGCTTGA‑TGATATCAGGCCAGGGCAGGGAAGGCGGCCAGTTACAAGGCTTGTAACCAAAGCCGGGCCCGTTCGCACAGGCCCGGCCGATGGCAACTATCAGAAGTAATAAGGGAATTTCAGGCTGAAAGAAAAGTCAGGTGCGTCATCGGTCAGACCAATGGACAGGTTGGGCACGATGGTCAGGTTGTCGGTGGCGGCGAAGGTCAGGCCAATATT  >  NC_002947/3255699‑3255992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑