Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,257,488 T→A 10.6% V155V (GTA→GTT) ‡ PP_2854 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,257,4880TA10.6% 144.5 / 4.4 52V155V (GTA→GTT) ‡PP_2854hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (21/25);  new base A (3/3);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCAGCAGGCCACCTTGGGCCAGGCGTTGCACGCTGCTGCCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCGTTGTTGTCACCGGCTGCACGGACCACCTGGGTTACACCTTCGGTGGTGGTCAGGCCGTTGCCGCCCGTTA  >  NC_002947/3257342‑3257633
                                                                                                                                                  |                                                                                                                                                 
tGCAGCAGGCCACCTTGGGCCAGGCGTTGCACGCTGCTGCCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTAc                                                                                                                                                 >  2:1908/1‑149 (MQ=255)
tGCAGCAGGCCACCTTGGGCCAGGCGTTGCACGCTGCTGCCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTAc                                                                                                                                                 >  4:125319/1‑149 (MQ=255)
   agcagGCCACCTTGGGCCAGGCGTTGCACGCTGCTGCCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGTACTGGCCGCCGGCCAGACCCTg                                                                                                                                              >  7:94045/1‑149 (MQ=255)
     cagGCCACCTTGGGCCAGGCGTTGCACGCTGCTGCCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGc                                                                                                                                            >  6:37239/1‑149 (MQ=255)
       ggCCACCTTGGGCCAGGCGTTGCACGCTGCTGCCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGTACTGGCCGCCGGCCAGACCCTggtcg                                                                                                                                          <  8:94045/149‑4 (MQ=255)
       ggCCACCTTGGGCCAGGCGTTGCACGCTGCTGCCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGTACTGGCCGCCGGCCAGACCCTggtcg                                                                                                                                          <  4:39527/149‑4 (MQ=255)
         ccACCTTGGGCCAGGCGTTGCACGCTGCTGCCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTg                                                                                                                                        <  8:137754/149‑1 (MQ=255)
          cACCTTGGGCCAGGCGTTGCACGCTGCTGCCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGc                                                                                                                                       >  1:156261/1‑149 (MQ=255)
            ccTTGGGCCAGGCGTTGCACGCTGCTGCCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCtg                                                                                                                                     <  2:156261/149‑1 (MQ=255)
              ttGGGCCAGGAGTTGCACTCTGCTGCCCTGGTTGTTGGTGGCATTGATGTTGAGCTGCACACCCAGACCGCTGGATGACTCACTGAGCGCGCCGGCGCCATTTTCCGCGTCCTGTGTCTGGGCGGCTGCCAGTACCTGGGCTTGCtgtt                                                                                                                                   <  8:212803/149‑1 (MQ=255)
                     aGGCGTTGCACGCTGCTGCCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTAcggc                                                                                                                            <  1:1908/149‑1 (MQ=255)
                        cGTTGCACGCTGCTGCCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCggg                                                                                                                         <  4:192710/149‑1 (MQ=255)
                          ttGCACGCTGCTGCCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGTACTGGCCGCCGGCCAGACCCTggtcg                                                                                                                                          >  4:203460/1‑127 (MQ=39)
                          ttGCACGCTGCTGCCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGTACTGGCCGCCGGCCAGACCCTggtcg                                                                                                                                          <  3:203460/130‑4 (MQ=39)
                              aCGCTGCTGCCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTg                                                                                                                   >  4:202818/1‑149 (MQ=255)
                                gctgctGTCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTg                                                                                                                                              <  7:227672/120‑1 (MQ=255)
                                gctgctGTCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTg                                                                                                                                              >  8:227672/1‑120 (MQ=255)
                                       cccTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCttt                                                                                                          <  2:99270/149‑1 (MQ=255)
                                        ccTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTg                                                                                                         <  2:194137/149‑1 (MQ=255)
                                        ccTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTg                                                                                                         <  8:189583/149‑1 (MQ=255)
                                        ccTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTg                                                                                                         <  5:37239/149‑1 (MQ=255)
                                         cTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCtgtt                                                                                                                                   <  4:145355/122‑1 (MQ=255)
                                         cTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCtgtt                                                                                                                                   >  3:145355/1‑122 (MQ=255)
                                                ttgCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGt                                                                                                  <  8:39636/148‑1 (MQ=255)
                                                  gCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGt                                                                                                                        <  2:72555/124‑1 (MQ=255)
                                                  gCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGt                                                                                                                        >  1:72555/1‑124 (MQ=255)
                                                    tGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGata                                                                                             >  7:112206/1‑149 (MQ=255)
                                                        aTTGATGTTGAGCTTCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCGGGTTGGCTTTGGTCACGTTGATATCg                                                                                          >  6:254812/1‑148 (MQ=255)
                                                        aTTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGtt                                                                                                                >  5:185218/1‑126 (MQ=255)
                                                        aTTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGtt                                                                                                                <  6:185218/126‑1 (MQ=255)
                                                               ttGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACgttgt                                                                                   <  1:240664/148‑1 (MQ=255)
                                                                  aGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTtgttgt                                                                                <  8:112206/148‑1 (MQ=255)
                                                                      gCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGTGTGCCTGGTTGGCTTTGGTCACGTTGATATCGCCGTGGTTGTAAgcg                                                                           >  8:121317/1‑149 (MQ=255)
                                                                              acaccGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGTCCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCgttgtt                                                                   >  1:229601/1‑149 (MQ=255)
                                                                                accGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGACAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCGttgttg                                                                  >  5:295545/1‑148 (MQ=255)
                                                                                             acacTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACgttgtt                                                                                  >  6:289931/1‑119 (MQ=255)
                                                                                             acacTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACgttgtt                                                                                  <  5:289931/119‑1 (MQ=255)
                                                                                                       cgcCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCGTTGTTGTCACCGGCTGCACGGACCACCTgg                                          <  5:254812/149‑1 (MQ=255)
                                                                                                        gcCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCGTTGTTGTCACCGGCTGCACGGACCACCTggg                                         <  3:125319/149‑1 (MQ=255)
                                                                                                                       cccGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCGTTGTTGTCACCGGCTGCACGGACCACCTGGGTTACACCTTCggtgg                          >  1:301770/1‑149 (MQ=255)
                                                                                                                        ccGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCGTTGTTGTCACCGGCTGCACGGACCACCTGGGTTACACCTTCggtggt                         >  3:51805/1‑149 (MQ=255)
                                                                                                                         cGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAgcgg                                                                          >  5:333212/1‑99 (MQ=255)
                                                                                                                         cGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAgcgg                                                                          <  6:333212/99‑1 (MQ=255)
                                                                                                                         cGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGACTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCGTTGTTGTCACCGGCTGCACGGACCACCTGGGTTACACCTTCggtggtg                        >  5:89084/1‑149 (MQ=255)
                                                                                                                         aggccAGGTACTGGCCGCCGGCCAGACCCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCGTTGTTGTCGCCGGCTGCACGGACCACCTGGGTTACACCTTCggtggtg                        >  7:253284/4‑149 (MQ=255)
                                                                                                                                     ggCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCGTTGTTGTCACCGGCTGCACGGACCACCTGGGTTACACCTTCGGTGGTGGTCAGGCCGtt             >  5:239248/1‑148 (MQ=255)
                                                                                                                                     ggCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCGTTGTTGTCACCGGCTGCACGGACCACCTGGGTTACACCTTCGGTGGTGGTCAGGCCGtt             <  6:239248/148‑1 (MQ=255)
                                                                                                                                      gCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCGTTGTTGTCACCGGCTGCACGGACCACCTGGGTTACACCTTCGGTGGTGGTCAGGCCGt              <  2:229601/146‑1 (MQ=255)
                                                                                                                                         ggcggcCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCGTTGTTGTCACCGGCTGCACGGACCACCTGGGTTACACCTTCGGTGGTGGTCAGGCCGTTgccgc        <  3:202818/149‑1 (MQ=255)
                                                                                                                                            ggcCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTCGTCACGTTGATATCGACGTTGTTGTAAGCGGCGTTGTTGTCACCGGCTGCACGGACCACCTGGGTTACACCTTCGGTGGTGGTCAGGCCGTTGCCGCCCg     <  7:121317/149‑1 (MQ=255)
                                                                                                                                              ccAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGGCACGTTGATATCGACGTTGTTGTAAGCGGCGTTGTTGTCACCGGCTGCACGGACCACCTGGGTTACACCTTCGGTGGTGGTCAGGCCGTTGCCGCCCGtt   <  2:160601/149‑1 (MQ=255)
                                                                                                                                               cAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCGTTGTTGTCACCGGCTGCACGGACCACCTGGGTTACACCTTCGGTGGTGGTCAGGCCGTTGCCGCCCGTTa  >  6:136239/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TGCAGCAGGCCACCTTGGGCCAGGCGTTGCACGCTGCTGCCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCGTTGTTGTCACCGGCTGCACGGACCACCTGGGTTACACCTTCGGTGGTGGTCAGGCCGTTGCCGCCCGTTA  >  NC_002947/3257342‑3257633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑