Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,274,310 T→A 11.1% I23N (ATC→AAC)  eamA → O‑acetylserine/cysteine export transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,274,3100TA11.1% 93.7 / 3.4 36I23N (ATC→AAC) eamAO‑acetylserine/cysteine export transporter
Reads supporting (aligned to +/- strand):  ref base T (16/16);  new base A (2/2);  total (18/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

AACTGTGCTGGTCCCTTCTCAAGGAATACCGTTATGCTGTAACGAAATATGTCTAAAAAGCCTGCAGAAGGAAGTCGCAATGCCACTCAAGGACCTGCTTATCGCCCTGGTGGTGATTGTCGCCTGGGGCGTCAACTTTGTGGTCATCAAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCATTGCGTTTCTTGCTGGTGGCGTTCCCGGCGATCTTGCTGGTCAGGCGGCCCAAACTGCCGTGGCGCTGGCTGATCGCTTACGGTGCGACCATTTCACTGGGCCA  >  NC_002947/3274164‑3274457
                                                                                                                                                  |                                                                                                                                                   
aaCTGTGCTGGTCCCTTCTCAAGGAATACCGTTATGCTGTAACGAAATATGTCTAAAAAGCCTGCAGAAGGAAGTCGCAATGCCACTCAAGGACCTGCTTATCGCCCTGGTGGTGATTGTCGCCTGGGGCGTCAACTTTGTGGtcatca                                                                                                                                                   <  7:190796/149‑1 (MQ=255)
           tCCCTTCTCAAGGAATACCGTTATGCTGTAACGAAATATGTCTAAAAAGCCTGCAGAAGGAAGTCGCAATGCCACTCAAGGACCTGCTTATCGCCCTGGTGGTGATTGTCGCCTGGGGCGTCAACTTTGTGGTCATCAAGGTTGTCCTc                                                                                                                                        <  4:67714/149‑1 (MQ=255)
             ccTTCTCAAGGAATACCGTTATGCTGTAACGAAATATGTCTAAAAAGCCTGCAGAAGGAAGTCGCAATGCCACTCAAGGACCTGCTTATCGCCCTGGTGGTGATTGTCGCCTGGGGCGTCAACTTTGTGGTCATCAAGGTTGGCCTCGa                                                                                                                                      <  8:232420/149‑1 (MQ=255)
               ttCTCAAGGAATACCGTTATGCTGTAACGAAATATGTCTAAAAAGCCTGCAGAAGGAAGTCGCAATGCCACTCAAGGACCTGCTTATCGCCCTGGTGGTGATTGTCGCCTGGGGCGTCAACTTTGTGGTCATCAAGGTTGGCCTCGACg                                                                                                                                    >  1:210182/1‑149 (MQ=255)
                    aaGGAATACCGTTATGCTGTAACGAAATATGTCTAAAAAGCCTGCAGAAGGAAGTCGCAATGCCACTCAAGGACCTGCTTATCGCCCTGGTGGTGATTGTCGCCTGGGGCGTCAACTTTGTGGTCATCAAGGTTGGCCTCGACGGGCt                                                                                                                                <  7:58495/148‑1 (MQ=255)
                                        aaCGAAATATGTCTAAAAAGCCTGCAGAAGGAAGTCGCAATGCCACTCAAGGACCTGCTTATCGCCCTGGTGGTGATTGTCGCCTGGGGCGTCAACTTTGTGGTCATCAAGGTTGGCCTCGACGGGCt                                                                                                                                <  4:307801/128‑1 (MQ=255)
                                        aaCGAAATATGTCTAAAAAGCCTGCAGAAGGAAGTCGCAATGCCACTCAAGGACCTGCTTATCGCCCTGGTGGTGATTGTCGCCTGGGGCGTCAACTTTGTGGTCATCAAGGTTGGCCTCGACGGGCt                                                                                                                                >  3:307801/1‑128 (MQ=255)
                                         aCGAAATATGTCTAAAAAGCCTGCAGAAGGAAGTCGCAATGCCACTCAAGGACCTGCTTATCGCCCTGGTGGTGATTGTCGCCTGGGGCGTCAACTTTGTGGTCATCAAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGc                                                                                                           <  4:45102/148‑1 (MQ=255)
                                         aCGAAATATGTCTAAAAAGCCTGCAGAAGGAAGTCGCAATGCCACTCAAGGACCTGCTTATCGCCCTGGTGGTGATTGTCGCCTGGGGCGTCAACTTTGTGGTCATCAAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCa                                                                                                          >  2:60569/1‑149 (MQ=255)
                                         aCGAAATATGTCTAAAAAGCCTGCAGAAGGAAGTCGCAATGCCACTCAAGGACCTGCTTATCGCCCTGGTGGTGATTGTCGCCTGGGGCGTCAACTTTGTGGTCAACAAGGTTGGCCTCGACGGGCTGCCa                                                                                                                            >  4:1853/1‑131 (MQ=255)
                                         aCGAAATATGTCTAAAAAGCCTGCAGAAGGAAGTCGCAATGCCACTCAAGGACCTGCTTATCGCCCTGGTGGTGATTGTCGCCTGGGGCGTCAACTTTGTGGTCAACAAGGTTGGCCTCGACGGGCTGCCa                                                                                                                            >  4:6362/1‑131 (MQ=255)
                                         aCGAAATATGTCTAAAAAGCCTGCAGAAGGAAGTCGCAATGCCACTCAAGGACCTGCTTATCGCCCTGGTGGTGATTGTCGCCTGGGGCGTCAACTTTGTGGTCAACAAGGTTGGCCTCGACGGGCTGCCa                                                                                                                            <  3:6362/131‑1 (MQ=255)
                                         aCGAAATATGTCTAAAAAGCCTGCAGAAGGAAGTCGCAATGCCACTCAAGGACCTGCTTATCGCCCTGGTGGTGATTGTCGCCTGGGGCGTCAACTTTGTGGTCAACAAGGTTGGCCTCGACGGGCTGCCa                                                                                                                            <  3:1853/131‑1 (MQ=255)
                                               tatGTCTAAAAAGCCTGCAGAAGGAAGTCGCAATGGCACTCAAGGACCTGCTTATCGCCGTGGTGGTGATTGTCGCCTGGGGCGTCAACTTTGTGGTCATCAAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCATTGCGt                                                                                                    <  3:119704/149‑1 (MQ=255)
                                                 tGTCTAAAAAGCCTGCAGAAGGAAGTCGCAATGCCACTCAAGGACCTGCTTATCGCCCTGGTGGTGATTGTCGCCTGGGGCGTCAACTTTGTGGTCATCAAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCATTGCGtt                                                                                                   <  1:60569/148‑1 (MQ=255)
                                                                cAGAAGGAAGTCGCAATGCCACTCAAGGACCTGCTTATCGCCCTGGTGGTGATTGTCGCCTGGGGCGTCAACTTTGTGGTCATCAAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCATTGCGTTTCTTGCTGGTGGCg                                                                                     <  4:59436/147‑1 (MQ=255)
                                                                 aGAAGGAAGTCGCAATGCCACTCAAGGACCTGCTTATCGCCCTGGTGGTGATTGTCGCCTGGGGCGTCAACTTTGTGGTCATCAAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCATTGCGTTTCTTGCTGGTGGCGTTc                                                                                  >  2:85939/1‑149 (MQ=255)
                                                                   aaggaagTCGCAATGCCACTCAAGGACCTGCTTATCGCCCTGGTGGTGATTGTCGCCTGGGGCGTCAACTTTGTGGTCATCAAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCATTGCGTTTCTTGCTGGTGGCGTTccc                                                                                >  5:280622/1‑149 (MQ=255)
                                                                        agTCGCAATGCCACTCAAGGACCTGCTTATCGCCCTGGTGGTGATTGTCGCCTGGGGCGTCAACTTTGTGGTCATCAAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCATTGCGTTTCTTGCTGGTGGCGTTCCCGGCGa                                                                           >  1:319255/1‑149 (MQ=255)
                                                                          tCGCAATGCCACTCAAGGACCTGCTTATCGCCCTGGTGGTGATTGTCGCCTGGGGCGTCAACTTTGTGGTCATCAAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCATTGCGTTTCTTGCTGGTGGCGTTCCCGGCGATc                                                                         >  5:268161/1‑149 (MQ=255)
                                                                            gCAATGCCACTCAAGGACCTGCTTATCGCCCTGGTGGTGATTGTCGCCTGGGGCGTCAACTTTGTGGTCATCAAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCATTGCGTTTCTTGCTGGTGGCGTTCCCGGCGATCtt                                                                       >  3:274448/1‑149 (MQ=255)
                                                                                            aCCTGCTTATCGCCCTGGTGGTGATTGTCGCCTGGGGCGTCAACTTTGTGGTCATCAAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCATTGCGTTTCTTGCTGGTGGCGtt                                                                                   <  3:198596/121‑1 (MQ=255)
                                                                                            aCCTGCTTATCGCCCTGGTGGTGATTGTCGCCTGGGGCGTCAACTTTGTGGTCATCAAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCATTGCGTTTCTTGCTGGTGGCGtt                                                                                   >  4:198596/1‑121 (MQ=255)
                                                                                                    aTCGCCCTGGTGGTGATTGTCGCCTGGGGCGTCAACTTTGTGGTCATCAAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCATTGCGTTTCTTGCTGGTGGCGTTCCCGGCGATCTTGCTGGTCAGGCGGCCCAAACTGc                                                <  4:274448/148‑1 (MQ=255)
                                                                                                        cccTGGTGGTGATTGTCGCCTGGGGCGTCAACTTTGTGGTCATCAAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCATTGCGTTTCTTGCTGGTGGCGTTCCCGGCGATCTTGCTGGTCAGGCGGCCCAAACTGCCGTgg                                           <  6:268161/149‑1 (MQ=255)
                                                                                                             gtggtgATTGTCGCCTGGGGCGTCAACTTTGTGGTCATCAAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCATTGCGTTTCTTGCTGGTGGCGTTCCCGGCGATCTTGCTGGTCAGGCGGCCCAAACTGCCGTGGCgctg                                      >  6:248056/1‑149 (MQ=255)
                                                                                                                   aTTGTCGCCTGGGGCGTCAACTTTGTGGTCATCAAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCATTGCGTTTCTTGCTGGTGGCGTTCCCGGCGATCTTGCTGGTCAGGCGGCCCAAACTGCCGTGGCGCTGGCTGAt                                >  8:145704/1‑149 (MQ=255)
                                                                                                                    ttGTCGCCTGGGGCGTCAACTTTGTGGTCATCAAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCATTGCGTTTCTTGCTGGTGGCGTTCCCGGCGATCTTGCTg                                                                   >  7:286335/1‑113 (MQ=255)
                                                                                                                    ttGTCGCCTGGGGCGTCAACTTTGTGGTCATCAAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCATTGCGTTTCTTGCTGGTGGCGTTCCCGGCGATCTTGCTg                                                                   <  8:286335/113‑1 (MQ=255)
                                                                                                                      gTCGCCTGGGGCGTCAACTTTGTGGTCATCAAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCATTGCGTTTCTTGCTGGTGGCGTTCCCGGCGATCTTGCTGGTCAGGCGGCCCAAACTGCCGTGGCGCTGGCTGATCGc                             >  5:240310/1‑149 (MQ=255)
                                                                                                                              gggCGTCAACTTTGTGGTCATCAAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCATTGCGTTTCTTGCTGGTGGCGTTCCCGGCGATCTTGCTGGTCAGGCGGCCCAAACTGCCGTGGCGCTGGCTGATCGCTTACGGTg                     >  1:232078/1‑149 (MQ=255)
                                                                                                                                     aaCTTTGTGGTCATCAAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCATTGCGTTTCTTGCTGGTGGCGTTCCCGGCGATCTTGCTGGTCAGGCGGCCCAAACTGCCGTGGCGCTGGCTGATCGCTTACGGTGCGACCa               <  1:85939/148‑1 (MQ=255)
                                                                                                                                        tttGTGGTCATCAAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCATTGCGTTTCTTGCTGGTGGCGTTCCCGGCGATCTTGCTGGTCAGGCGGCCCAAACTGCCGTGGCGCTGGCTGATCGCTTACGGTGCGACCAttt            >  4:22808/1‑148 (MQ=255)
                                                                                                                                             ggTCATCAAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCATTGCGTTTCTTGCTGGTGGCGTTCCCGGCGATCTTGCTGGTCAGGCGGCCCAAACTGCCGTGGCGCTGGCTGATCGCTTa                          >  1:194922/1‑129 (MQ=255)
                                                                                                                                             ggTCATCAAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCATTGCGTTTCTTGCTGGTGGCGTTCCCGGCGATCTTGCTGGTCAGGCGGCCCAAACTGCCGTGGCGCTGGCTGATCGCTTa                          <  2:194922/129‑1 (MQ=255)
                                                                                                                                              gTCATCAAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCATTGCGTTTCTTGCTGGTGGCGTTCCCGGCGATCTTGCTGGTCAGGCGGCCCAAACTGCCGTGGCGCTGGCTGATCGCTTACGGTGCGACCATTTCACTggg     <  2:319255/149‑1 (MQ=255)
                                                                                                                                              gTCATCAAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCATTGCGTTTCTTGCTGGTGGCGTTCCCGGCGATCTTGCTGGTCAGGCGGCCCAAACTGCCGTGGCGCTGGCTGATCGCTTACGGTGCGACCATTTCACTgg      <  6:240310/148‑1 (MQ=255)
                                                                                                                                                 atcaAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCATTGCGTTTCTTGCTGGTGGCGTTCCCGGCGATCTTGCTGGTCAGGCGGCCCAAACTGCCGTGGCGCTGGCTGATCGCTTACGGTGCGACCATTTCACTgg      <  7:190744/145‑1 (MQ=255)
                                                                                                                                                 atcaAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCATTGCGTTTCTTGCTGGTGGCGTTCCCGGCGATCTTGCTGGTCAGGCGGCCCAAACTGCCGTGGCGCTGGCTGATCGCTTACGGTGCGACCATTTCACTgg      >  8:190744/1‑145 (MQ=255)
                                                                                                                                                 atcaAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCATTGCGTTTCTTGCTGGTGGCGTTCCCGGCGATCTTGCTGGTCAGGCGGCCCAAACTGCCGTGGCGCTGGCTGATCGCTTACGGTGCGACCATTTCACTGGGc    >  1:12972/1‑147 (MQ=255)
                                                                                                                                                 atcaAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCATTGCGTTTCTTGCTGGTGGCGTTCCCGGCGATCTTGCTGGTCAGGCGGCCCAAACTGCCGTGGCGCTGGCTGATCGCTTACGGTGCGACCATTTCACTGGGCCa  >  8:84133/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AACTGTGCTGGTCCCTTCTCAAGGAATACCGTTATGCTGTAACGAAATATGTCTAAAAAGCCTGCAGAAGGAAGTCGCAATGCCACTCAAGGACCTGCTTATCGCCCTGGTGGTGATTGTCGCCTGGGGCGTCAACTTTGTGGTCATCAAGGTTGGCCTCGACGGGCTGCCACCGATGTTGCTGGGGGCATTGCGTTTCTTGCTGGTGGCGTTCCCGGCGATCTTGCTGGTCAGGCGGCCCAAACTGCCGTGGCGCTGGCTGATCGCTTACGGTGCGACCATTTCACTGGGCCA  >  NC_002947/3274164‑3274457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑