Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,372,047 T→C 7.7% Q9R (CAG→CGG)  csoR ← copper utilization transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,372,0470TC 7.7% 146.7 / 2.9 52Q9R (CAG→CGG) csoRcopper utilization transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (21/27);  new base C (2/2);  total (23/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01

GGCCTGGCAGACGGCTTTTTCCACGGCATGCAGTTGCTGAGCGATGTCGACGCAGGCCCGGCTGTCTTCGATCATGCTGACGATGCTGCGCAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAA  >  NC_002947/3371902‑3372183
                                                                                                                                                 |                                                                                                                                        
ggCCTGGCAGACGGCTTTTTCCACGGCATGCAGTTGCTGAGCGATGTCGACGCAGGCCCGGCTGTCTTCGATCATGCTGACGATGCTGCGCAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGa                                                                                                                                        <  7:43795/148‑1 (MQ=255)
  ccTGGCAGACGGCTTTTTCCACGGCATGCAGTTGCTGAGCGATGTCGACGCAGGCCCGGCTGTCTTCGATCATGCTGACGATGCTGCGCAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATgg                                                                                                                                     <  1:119596/149‑1 (MQ=255)
     ggCAGACGGCTTTTTCCACGGCATGCAGTTGCTGAGCGATGTCGACGCAGGCCCGGCTGTCTTCGATCATGCTGACGATGCTGCGCAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGCCg                                                                                                                                  >  1:42628/1‑149 (MQ=255)
      gCAGACGGCTTTTTCCACGGCATGCAGTTGCTGAGCGATGTCGACGCAGGCCCGGCTGTGTTCGATCATGCTGACGATGCTGCGCAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGcc                                                                                                                                   <  5:97347/147‑1 (MQ=255)
      gCAGACGGCTTTTTCCACGGCATGCAGTTGCTGAGCGATGTCGACGCAGGCCCGGCTGTCTTCGATCATGCTGACGATGCTGCGCAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGcc                                                                                                                                   <  5:194804/147‑1 (MQ=255)
      gCAGACGGCTTTTTCCACGGCATGCAGTTGCTGAGCGATGTCGACGCAGGCCCGGCTGTCTTCGATCATGCTGACGATGCTGCGCAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGCCgt                                                                                                                                 <  7:151286/149‑1 (MQ=255)
              gtttttCCACGGCATGCAGTTGCTGAGCGATGTCGACGCAGGCCCGGCTGTCTTCGATCATGCTGACGATGCTGCGCAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGa                                                                                                                         >  1:131293/2‑149 (MQ=255)
                  ttCCACGGCATGCAGTTGCTGAGCGATGTCGACGCAGGCCCGGCTGTCTTCGATCATGCTGACGATGCTGCGCAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCg                                                                                                                      <  6:222786/148‑1 (MQ=255)
                     cACGGCATGCAGTTGCTGAGCGATGTCGACGCAGGCCCGGCTGTCTTCGATCATGCTGACGATGCTGCGCAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTc                                                                                                                   <  1:288818/148‑1 (MQ=255)
                               aGTTGCTGAGCGATGTCGACGAAGGCCAGGCTGTCTTCGATCATGCTGACGATGCTGCGCAGGAGGCCCACCGCACGCTTCAACCTTTTCATGATATCGCTATGACGTTTCTGCTGATGGCCGTCTTCGTGATCCATAATACCTTGAGt                                                                                                        >  3:63685/1‑149 (MQ=255)
                                                gACGCAGGCCCGGCTGTCTTCGATCATGCTGACGATGCTGCGCAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCAt                                                                                               >  4:310745/1‑141 (MQ=255)
                                                gACGCAGGCCCGGCTGTCTTCGATCATGCTGACGATGCTGCGCAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCAt                                                                                               <  3:310745/141‑1 (MQ=255)
                                                gACGCAGGCCCGGCTGTCTTCGATCATGCTGACGATGCTGCGCAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGaa                                                                                        <  2:42628/148‑1 (MQ=255)
                                                gACGCAGGCCCGGCTGTCTTCGATCATGCTGACGATGCTGCGCAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAAt                                                                                       >  7:38189/1‑149 (MQ=255)
                                                 aCGCAGGCCCGGCTGTCTTCGATCATGCTGACGATGCTGCGCAGGTGGCCCTCCGCACGCTTCAACCTTATGATGATATCGCTATGACTTTGGTGCTGATGGCCGTGTGCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAAtt                                                                                      <  2:265817/149‑1 (MQ=255)
                                                  cGCAGGCCCGGCTGTCTTCGATCATGCTGACGATGCTGCGCAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTa                                                                                     <  8:38189/149‑1 (MQ=255)
                                                   gCAGGCCCGGCTGTCTTCGATCATGCTGACGATGCTGCGCAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTAc                                                                                    >  6:274304/1‑149 (MQ=255)
                                                    cAGGCCCGGCTGTCTTCGATCATTCTGACGATGCTGCGCAGGTTGCCATCCGCACTCTTCAACCTTTTGATGATATAGCTATGACTTTGGTGCTTATGGCAGTGTTCGTTATCGCTCATGACTTGAGTCTCATGCATCTATGAATTACg                                                                                   <  7:200962/149‑1 (MQ=255)
                                                     aGGCCCGGCTGTCTTCGATCATGCTGACGATGCTGCGCAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGc                                                                                  >  3:114843/1‑149 (MQ=255)
                                                         ccGGCTGTCTTCGATCATGCTGACGATGCTGCGCAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGcc                                                                                                                                   >  6:136396/1‑96 (MQ=255)
                                                         ccGGCTGTCTTCGATCATGCTGACGATGCTGCGCAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGcc                                                                                                                                   <  5:136396/96‑1 (MQ=255)
                                                         ccGGCTGTCTTCGATCATGCTGACGATGCTGCGCAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCGTGCCTTGAGTCTCAt                                                                                                   >  5:196146/1‑128 (MQ=255)
                                                         ccGGCTGTCTTCGATCATGCTGACGATGCTGCGCAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCGTGCCTTGAGTCTCAt                                                                                                   <  6:196146/128‑1 (MQ=255)
                                                         ccGGCTGTCTTCGATCATGCTGACGATGCTGCGCAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCg                                                                                             <  4:101313/134‑1 (MQ=255)
                                                         ccGGCTGTCTTCGATCATGCTGACGATGCTGCGCAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCg                                                                                             >  3:101313/1‑134 (MQ=255)
                                                         ccGGCTGTCTTCGATCATGCTGACGATGCTGCGCAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCg                                                                               >  7:48646/1‑148 (MQ=255)
                                                                    cGATCATGCTGACGATGCTGCGCAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCCGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATcccc                                                                   >  1:179798/1‑149 (MQ=255)
                                                                    cGATCATGCTGACGATGCTGCGCAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCCGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCATATcccc                                                                   >  1:184622/1‑149 (MQ=255)
                                                                              gACGATGCTGCGCAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCAt                                                                                                   <  6:279674/107‑1 (MQ=255)
                                                                              gACGATGCTGCGCAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCAt                                                                                                   >  5:279674/1‑107 (MQ=255)
                                                                                     ctgcGCAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTGATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTTGGGGGGATATGGt                                                   >  5:64609/1‑148 (MQ=255)
                                                                                      tgcGCAGGTGGCCCTCCGCAAGCTTCAACATTTTGATGATATCGCTATGACTTTGGTGCTGATGGAAGTGTTCTTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCACCCTGGAGGGGATATGGTc                                                  >  3:177009/1‑148 (MQ=255)
                                                                                        cgcAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCaaa                                               <  4:114843/149‑1 (MQ=255)
                                                                                          cAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCCGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAAcc                                             <  2:179798/149‑1 (MQ=255)
                                                                                          cAGGTGGCACTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCCGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGTGATATGGTCAAAcc                                             <  2:184622/149‑1 (MQ=255)
                                                                                             gTGGCCCTCCGCAGTCTTCAATCTTTTGATGATATAGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAAc                                          <  4:63685/149‑1 (MQ=255)
                                                                                              tGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAAc                                          >  2:264043/1‑148 (MQ=255)
                                                                                                gCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTAc                                       >  4:1979/1‑149 (MQ=255)
                                                                                                  ccTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTAccc                                     >  2:16599/1‑149 (MQ=255)
                                                                                                       gCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCa                                <  1:264043/149‑1 (MQ=255)
                                                                                                             gtCACCCTTTTGATGATAACGCTATGACTTTGGAGCTGATGGCCGTGTTCGTGATCGCTCAAGCCTGGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGaacaa                          <  4:38664/148‑1 (MQ=255)
                                                                                                              tccaCCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGaacaac                         >  8:101809/4‑149 (MQ=255)
                                                                                                                aaCCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTc                                                  <  5:66669/122‑1 (MQ=255)
                                                                                                                aaCCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTc                                                  >  6:66669/1‑122 (MQ=255)
                                                                                                                         atgatATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATTCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTa               <  7:101809/148‑1 (MQ=255)
                                                                                                                          tgttatCTCTATGACTTTGGTGCTGATTGCCGTGTTCGTTATCGCTCATGCCGTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAg              <  6:64609/145‑1 (MQ=255)
                                                                                                                          tgatATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGACTCATGCATCGATGAATTACGCTCGAATCCTATCACCCTGGGGGGGATATGGTCAAACCAAATACCCATTCACACAAACCATCCGCtagat             >  3:28212/1‑145 (MQ=255)
                                                                                                                               tCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATc         <  3:1979/148‑1 (MQ=255)
                                                                                                                                cGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGaacaac                         >  4:86514/1‑131 (MQ=255)
                                                                                                                                cGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGaacaac                         <  3:86514/131‑1 (MQ=255)
                                                                                                                                 gCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCGCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCaaa      <  6:148828/149‑1 (MQ=255)
                                                                                                                                     tGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGaa  <  5:274304/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                        
GGCCTGGCAGACGGCTTTTTCCACGGCATGCAGTTGCTGAGCGATGTCGACGCAGGCCCGGCTGTCTTCGATCATGCTGACGATGCTGCGCAGGTGGCCCTCCGCACGCTTCAACCTTTTGATGATATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAA  >  NC_002947/3371902‑3372183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑