| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,466,843 | C→A | 11.8% | intergenic (‑18/+106) | PP_3081 ← / ← PP_5560 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,466,843 | 0 | C | A | 11.8% | 88.0 / 4.9 | 42 | intergenic (‑18/+106) | PP_3081/PP_5560 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (13/24); new base A (2/3); total (15/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.85e-01 | |||||||||||
GTACCTGCGGGCAGGTTGTCCCAGCTCAGTTGCGGGGT‑CCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGC‑GTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCTCGGCAGTGCCTCCGCTG > NC_002947/3466695‑3466985 | gTACCTGCGGGCAGGTTGTCCCAGCTCAGTTGCGGGGT‑CCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCTgc < 3:247872/149‑1 (MQ=255) ccTGCGGGCAGGTTGTCCCAGCTCAGTTGCGGGGTACCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCTgcgg > 1:48727/1‑149 (MQ=255) ccTGCGGGCAGGTTGTCCCAGCTCAGTTGCGGGGT‑CCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCTgcggc < 4:90005/149‑1 (MQ=255) gCGGGCAGGTTGTCCCAGCTCAGTTGCGGGGT‑CCGCTCTTCACCACCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCTGCggcggc > 1:229460/1‑149 (MQ=255) gggCAGGTTGTCCCAGCTCAGTTGCGGGGT‑CCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCGCAGGCGGcc > 5:138265/1‑148 (MQ=255) gCAGGTTGTCCCAGCTCAGTTGCGGGGT‑CCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCGCAGGCGGCCaag < 7:20775/149‑2 (MQ=255) gCAGGTTGTCCCAGCTCAGTTGCGGGGT‑CCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCGCAGGCGGCCaag < 6:138265/149‑2 (MQ=255) gTTGTCCCAGCTCAGTTGCGGGGT‑CCGCTCTTCACCGGCACCGAATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCtg < 2:229460/149‑1 (MQ=255) tCCCAGCTCAGTTGCGGGGG‑CCGCTCTTCACCGGCACCGCAGGCGGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTTAAAGACTAGTAACT‑CACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGc < 2:123915/148‑1 (MQ=255) cccAGCTCAGTTGGGGGGT‑CCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGCCCTGTTGCAGGGCAATTACCCCGCCATCAGTTAAACACGAGGAAATACACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCa < 1:228974/149‑1 (MQ=255) ccAGCTCAGTTGCGGGGT‑CCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCGTTGGCCGCCGCAGGCGGCCAAGGcgcgatg > 3:52800/1‑141 (MQ=255) ccAGCTCAGTGGCGGGGG‑CCGCTCTACCCCGGCCCCGCATGCGGGGTCCTGGCCGACCTGTTGCTGGGCAATGACGCCGCCATCAGTCAACGACGCGCAACT‑CACCGACATCGCGGGTTGGCCGCCGCAGGCGGCCAAGGcgcgatg < 4:52800/147‑7 (MQ=255) cAGCTCAGTTGCGGGGT‑CCGCTCTTCACCGGCACCGCATGTCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAggg < 1:218091/149‑1 (MQ=255) cAGCTCAGTTGCGGGGT‑CCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCAGCGGCGGCCAACCCTGTTCAggg > 4:63612/1‑149 (MQ=255) cAGCTCAGTTGCGGGGT‑CCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCCCCGGCGGCGGCCCACCCAGTGCAggg > 2:147839/1‑149 (MQ=255) aGCTCAGTTGCGGGGT‑CCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAggg > 8:67881/1‑148 (MQ=255) aGCTCAGTTGCGGGGT‑CCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCGGCGGCGGCCAACGCTGTGCAGGgc > 3:187500/1‑149 (MQ=255) cTCAGTTGCGGGGT‑CCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCGGCGGCGGCCCACCCGGTGGAGGggag > 2:190365/1‑149 (MQ=255) cGGGGT‑CCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAagcag < 1:190365/149‑1 (MQ=255) cGGGGT‑CCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAagca < 2:198034/148‑1 (MQ=255) ggT‑CCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGa < 2:48727/149‑1 (MQ=255) ccGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACa < 7:67881/149‑1 (MQ=255) cGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAg > 1:34000/1‑149 (MQ=255) ttCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCTGcggcgg > 3:210785/1‑110 (MQ=255) ttCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCTGcggcgg < 4:210785/110‑1 (MQ=255) aCCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCCACGCAGTGCAGGGCAGCAAAGCAGCGAACAGCCAgtgtg > 6:92987/1‑148 (MQ=255) ggaccccGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTg < 8:83722/144‑1 (MQ=255) ccGGGTCCTGGCCGACCTGTTGCAGGGAAATGACGCCGCAATCTGTCAAATCCGAGCAACT‑GACCGACATCGCGCCTTGGCCGCCGGCGGGGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGt < 4:161224/148‑1 (MQ=255) cGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCa < 5:92987/149‑1 (MQ=255) gggTCCTGGCCGACCTGTTGCTGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAagcagc < 2:249320/119‑1 (MQ=255) ggTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCtgtg > 4:214733/1‑101 (MQ=255) ggTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCtgtg < 3:214733/101‑1 (MQ=255) gtgcccGACGTGTTCCAGGGCTAGTACGCCCCCAACAGTGACCCACGCGTAACT‑GCCCGACTTCGCGCCTTGGCCGCCTGCGGGGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCtt < 8:262579/145‑1 (MQ=255) ctaCCTGTTGCAGGGCAATGAGGCCGCCATCAGTGAAATACGCGGAACT‑GACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAg < 4:228262/147‑1 (MQ=255) ccTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGc < 3:116610/149‑1 (MQ=255) ttGCAGGGCAATGACGCCGCCATCAGTCAAAGACGAGCAACT‑GACCGACAGCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGC‑GTc < 1:147839/148‑1 (MQ=255) tGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGC‑GTCAc > 7:249388/1‑149 (MQ=255) gCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGC‑GTCAc < 2:77040/148‑1 (MQ=255) gCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGC‑GTCAc > 1:77040/1‑148 (MQ=255) gggCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGC‑GTCACGGCt < 4:187500/149‑1 (MQ=255) gCATTGACTCCGCCATCAGTTAAAGACGAGGAACT‑GACCTACATCTCGCCTTGTCCGCCTGCGGCGGCCAACGCTGTGCAGTGCATCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGC‑GTCACGGCTCa < 6:302480/149‑1 (MQ=255) tGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGGCGCCGGCGGCCGCCAACCCGGTGGACGGCAGCAAAGCAGCGAACAGCCAGGGTGTGAGGGGCATGGGCAAAACAAGGGAGGGCGGGCAC‑GCGCAGTGTAGACCACGTCTGcgt > 4:17217/1‑147 (MQ=255) aaGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGC‑GTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAg < 8:249388/149‑1 (MQ=255) aCGAGCAAGT‑GACCGACATCGCGCGTTGGCCGCGGGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGC‑GTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCgg < 3:140242/149‑1 (MQ=255) aCATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGC‑GTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCTCg < 2:34000/149‑1 (MQ=255) ggCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCATGGCAGGGC‑GTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCTCGGCAGTGCCTCCg > 3:313855/1‑149 (MQ=255) cgccTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGC‑GTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCTCGGCAGTGCCTCCGCTg < 3:63612/149‑1 (MQ=255) | GTACCTGCGGGCAGGTTGTCCCAGCTCAGTTGCGGGGT‑CCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACT‑GACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGC‑GTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCTCGGCAGTGCCTCCGCTG > NC_002947/3466695‑3466985 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |