Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,498,252 A→G 7.1% intergenic (‑656/+50) PP_5562 ← / ← PP_5563 hypothetical protein/membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,498,2520AG 7.1% 158.4 / 2.9 56intergenic (‑656/+50)PP_5562/PP_5563hypothetical protein/membrane protein
Reads supporting (aligned to +/- strand):  ref base A (26/26);  new base G (2/2);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

GCAGCCGCCACCGCATTCGCGGGCAAGCCCGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACACCCG  >  NC_002947/3498111‑3498397
                                                                                                                                             |                                                                                                                                                 
gCAGCCGCCACCGCATTCGCGGGCAAGCCCGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCgg                                                                                                                                            >  1:188199/1‑149 (MQ=255)
  aGCCGCCACCGCATTCGCGGGCAAGCCCGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGc                                                                                                                                          >  1:131657/1‑149 (MQ=255)
     ctccACCGCATTCGCGGGCAATCCCGCTACTACATCGTGCGGTGGTGTCGCCAGGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGc                                                                                                                                       <  6:326665/147‑1 (MQ=25)
        cACCGCATTCGCGGGCAAGCCCGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTg                                                                                                                                    <  4:96208/149‑1 (MQ=255)
         acggCATTCGCGGGCAAGCCCGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGc                                                                                                                                   <  5:170500/146‑1 (MQ=255)
         aCCGCATTCGCGGGGAAGCCCGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGc                                                                                                                                   >  7:273600/1‑149 (MQ=255)
                tCGCGGGCAAGCCCGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGa                                                                                                                             >  1:178106/1‑148 (MQ=255)
                tCGCGGGCAAGCCCGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGa                                                                                                                             <  2:178106/148‑1 (MQ=255)
                     ggCAAGCCCGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCg                                                                                                                       <  2:188199/149‑1 (MQ=255)
                           cccGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAg                                                                                                                 >  5:311980/1‑149 (MQ=255)
                            ccGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGgc                                                                                                                     >  1:211290/1‑144 (MQ=255)
                            ccGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGgc                                                                                                                     <  2:211290/144‑1 (MQ=255)
                            ccGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCa                                                                                                                  <  8:13771/147‑1 (MQ=255)
                            ccGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCa                                                                                                                  >  7:13771/1‑147 (MQ=255)
                              gCTCCAACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCg                                                                                                              >  5:67369/1‑149 (MQ=255)
                                    aCATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCa                                                                                                        >  3:251806/1‑149 (MQ=255)
                                      aTCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGtt                                                                                                           <  8:130231/144‑1 (MQ=255)
                                      aTCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGtt                                                                                                           >  7:130231/1‑144 (MQ=255)
                                      aTCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAAAACCGGCTGCGCCGGtgttcgc                                                                                                      <  2:131657/149‑7 (MQ=21)
                                          aCCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCGTTCGCGGGCAGGCTGCCCGCGaatgcgg                                                                                                                       >  8:144462/1‑123 (MQ=255)
                                          aCCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCGTTCGCGGGCAGGCTGCCCGCGaatgcgg                                                                                                                       <  7:144462/128‑6 (MQ=255)
                                          aCCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCGTTCGCGGGCAGGCTGCCCGCGaatgcgg                                                                                                                       >  8:148720/1‑123 (MQ=255)
                                          aCCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCGTTCGCGGGCAGGCTGCCCGCGaatgcgg                                                                                                                       <  7:148720/128‑6 (MQ=255)
                                             ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGACACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCa                                                                                               <  6:311980/149‑1 (MQ=255)
                                              gtggtgTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATcc                                                                                                     >  3:97033/1‑142 (MQ=255)
                                              gtggtgTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATcc                                                                                                     <  4:97033/142‑1 (MQ=255)
                                              ctggtggAGCCACGTTGGAGTCGCTGGTTTACACGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGGCAATCACCGTCACAGCCGCCACCCCATTCGCGGTCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCaa                                                                                              <  7:111850/148‑1 (MQ=25)
                                                           gTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCt                                                                                 >  3:125298/1‑149 (MQ=255)
                                                              gTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCCGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTa                                                                              >  8:114147/1‑149 (MQ=255)
                                                                            aCCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTctct                                                                 <  5:174247/148‑1 (MQ=255)
                                                                              ccGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGc                                                               >  7:276515/1‑148 (MQ=255)
                                                                              ccGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGc                                                               >  8:11122/1‑148 (MQ=255)
                                                                              ccGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGc                                                               >  5:79271/1‑148 (MQ=255)
                                                                               cgcgAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCa                                                             >  7:55728/1‑149 (MQ=255)
                                                                                  gAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCg                                                          <  4:251806/149‑1 (MQ=255)
                                                                                     tAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCTACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGAtt                                                       <  6:67369/149‑1 (MQ=255)
                                                                                     tAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGAtt                                                       <  8:276515/149‑1 (MQ=255)
                                                                                     tAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGAtt                                                       <  5:217929/149‑1 (MQ=255)
                                                                                          cGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCtt                                                  <  4:125298/149‑1 (MQ=255)
                                                                                            gcaggtccGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGc                                                <  1:167927/143‑1 (MQ=255)
                                                                                            gACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGc                                                <  7:114147/149‑1 (MQ=255)
                                                                                                  ccGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCAt                                                            >  6:25354/1‑131 (MQ=255)
                                                                                                  ccGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCAt                                                            <  5:25354/131‑1 (MQ=255)
                                                                                                        aCTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACg                                                                                            >  2:259500/1‑93 (MQ=255)
                                                                                                        aCTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACg                                                                                            <  1:259500/93‑1 (MQ=255)
                                                                                                            tCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGtt                                <  8:273600/149‑1 (MQ=255)
                                                                                                                  gTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATg                            <  6:79271/147‑1 (MQ=255)
                                                                                                                  gTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTAGATga                           >  7:88511/1‑147 (MQ=255)
                                                                                                                     aTTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCCGCCGGTTTCATCCAACTCAACGACCATCGCCCTGTACGCAAACGTCTCTGGCATCGATTGCCGTGCATGGCCAAGTGTCGGGGATGTAAt                        >  4:7272/1‑148 (MQ=255)
                                                                                                                     aTTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAAt                        >  6:134335/1‑148 (MQ=255)
                                                                                                                         aCCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATcc                   >  4:234383/1‑149 (MQ=255)
                                                                                                                                 aGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATc                                                                                      <  5:245664/74‑1 (MQ=255)
                                                                                                                                 aGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATc                                                                                      >  6:245664/1‑74 (MQ=255)
                                                                                                                                        aCCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGc                <  4:206064/137‑1 (MQ=255)
                                                                                                                                        aCCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGc                >  3:206064/1‑137 (MQ=255)
                                                                                                                                          cGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACACCCg  >  3:283154/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                 
GCAGCCGCCACCGCATTCGCGGGCAAGCCCGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACACCCG  >  NC_002947/3498111‑3498397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑