| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,557,606 | G→A | 12.1% | intergenic (+83/‑96) | PP_3141 → / → PP_3142 | WecB/TagA/CpsF family glycosyl transferase/sugar transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,557,606 | 0 | G | A | 12.1% | 80.1 / 3.4 | 33 | intergenic (+83/‑96) | PP_3141/PP_3142 | WecB/TagA/CpsF family glycosyl transferase/sugar transferase |
| Reads supporting (aligned to +/- strand): ref base G (15/14); new base A (2/2); total (17/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.15e-01 | |||||||||||
GCTATTCCTGTTGCGTACCCTGTTGTACCGGCGGGCGGCGGTTTCGGCCAGGCGGGCCCTATGAATCGGCCAAAAATCGCAAAAGCGGTGATCCC‑GGGTACGCCAGCGCCGGGTGGCTGCTACAGTGATATCAATCGCTTGAGGGCGCCACGAGCCTGCTGCACGGTTTTTGCTTAAGTCGGTTTTCAAGACTTGCTGTTTGA‑TGGAAGGCACTTGCTACGCTCATCTTGATGAGGTCTGAAATGGTTTTCGAACCCAGAAGCAGTCGTTCCTTACTCCAGAGAAGAAGCAG > NC_002947/3557460‑3557751 | gCTATTCCTGTTGCGTACCCTGTTGTACCGGCGGGCGGCGGTTTCGGCCAGGCGGGCCCTATGAATCGGCCAAAAATCGCAAAAGCGGTGATCCC‑GGGTACGCCAGCGCCGGGTGGCTGCTACAGTGATATCAATCGCTTGAGGGCGcc < 7:265312/149‑1 (MQ=255) tGTTGCGTACCCTGTTGTACCGGCGGGCGGCGGTTTCGGCCAGGCGGGCCCTATGAATCGGCCAAAAATCGCAAAAGCGGTGATCCC‑GGGTACGCCAGCGCCGGGTGGCTGCTACAGTGATATCAATCGCTTGAGGGCGcc < 8:47472/141‑1 (MQ=255) tGTTGCGTACCCTGTTGTACCGACGGGCGGCGGTTTCGGCCAGGCGGGCCCTATGAATCGGCCAAAAATCGCAAAAGCGGTGATCCC‑GGGTACGCCAGCGCCGGGTGGCTGCTACAGTGATATCAATCGCTTGAGGGCGcc > 7:47472/1‑141 (MQ=255) cccTGTTGTACCGGCGGGCGGCGGTTTCGGCCAGGCGGGCCCTATGAATCGGCCAAAAATCGCAAAAGCGGTGATCCC‑GGGTACGCCAGCGCCGGGTGGCTGCTACAGTGATATCAATCGCTTGAGGGCGCCACGAGCCTGCTGCACgg > 7:187467/1‑149 (MQ=255) ccGGCGGGCGGCGGTTTCGGCCAGGCGGGCCCTATGAATCGGCCAAAAATCGCAAAAGCGGTGATCCC‑GGGTACGCCAGCGCCGGGTGGCTGCTACAGTGATATCAATCGCTTGAGGGCGCCACGAGCCTGCTGCACGGTTTTTGCtt < 6:52736/148‑1 (MQ=255) ccGGCGGGCGGCGGTTTCGGCCAGGCGGGCCCTATGAATCGGCCAAAAATCGCAAAAGCGGTGATCCC‑GGGTACGCCAGCGCCGGGTGGCTGCTACAGTGATATCAATCGCTTGAGGGCGCCACGAGCCTGCTGCACGGTTTTTGCtt > 1:45560/1‑148 (MQ=255) ggCCAGGCGGGCCCTATGAATCGGCCAAAAATCGCAAAAGCGGTGATCCC‑GGGTACGCCAGCGCCGGGTGGCTGCTACAGTGATATCAATCGCTTGAGGGCGCCACGAGCCTGCTGCACGGTTTTTGCTTAAGTCGGTTTTCAAGACtt > 4:31860/1‑149 (MQ=255) ccAGGCGGGCCCTATGAATCGGCCAAAAATCGCAAAAGCGGTGATCCC‑GGGTACGCCAGCGCCGGGTGGCTGCTACAGTGATATCAATCGCTTGAGGGCGCCACGAGCCTGCTGCACGGttttt < 4:45978/124‑1 (MQ=255) ccAGGCGGGCCCTATGAATCGGCCAAAAATCGCAAAAGCGGTGATCCC‑GGGTACGCCAGCGCCGGGTGGCTGCTACAGTGATATCAATCGCTTGAGGGCGCCACGAGCCTGCTGCACGGttttt > 3:45978/1‑124 (MQ=255) cTATGAATCGGCCAAAAATCGCAAAAGCGGTGATCCC‑GGGTACGCCAGCGCCGGGTGGCTGCTACAGTGATATCAATCGCTTGAGGGCGCCACGAGCCTGCTGCACGGTTTTTGCTTAAGTCGGTTTTCAAGACTTGCTGTTTGA‑TGGa < 1:302661/149‑1 (MQ=255) tATGAATCGGCCAAAAATCGCAAAAGCGGTGATCCCAGGGTACGCCAGCGCCGGGTGGCTGCTACAGTGATATCAATCGCTTGAGGGCGCCACGAGCCTGCTGCACGGTTTTTGCTTAAGTCGGTTTTCAAGACTTACTGTTTGA‑Tgg > 1:214358/1‑148 (MQ=255) aaGCGGTGATCCC‑GGGTACGCCAGCGCCGGGTGGCTGCTACAGTGATATCAATCGCTTGAGGGCGCCACGAGCCTGCTGCACGGTTTTTGCTTAAGTCGGTTTTCAAGACTTGATGTTTGA‑TGGAAGGCACTTGCTACGCTCATCTtga > 7:171546/1‑149 (MQ=255) aTCCC‑GGGTACGCCAGCGCCGGGTGGCTGCTACAGTGATATCAATCGCTTGAGGGCGCCACGAGCCTGCTGCACGGTTTTTGCTTAAGTCGGTTTTCAAGACTTGCTGTTTGA‑TGGAAGGCACTTGCTa > 8:146412/1‑129 (MQ=255) aTCCC‑GGGTACGCCAGCGCCGGGTGGCTGCTACAGTGATATCAATCGCTTGAGGGCGCCACGAGCCTGCTGCACGGTTTTTGCTTAAGTCGGTTTTCAAGACTTGCTGTTTGA‑TGGAAGGCACTTGCTa < 7:146412/129‑1 (MQ=255) c‑GGGTACGCCAGCGCCGGGTGGCTGCTACAGTGATATCAATCGCTTGAGGGCGCCACGAGCCTGCTGCACGGTTTTTGCTTAAGTCGGTTTTCAAGACTTGCTGTTTGA‑TGGAAGGCACTTGCTACGCTCATCTTGATGAGGTCTGaaa > 2:185284/1‑149 (MQ=255) gTACGCCAGCGCCGGGTGGCTGCTACAGTGATATCAATCGCTTGAGGGCGCCACGAGCCTGCTGCACGGTTTTTGCTTAAGTCGGTTTTCAAGACTTGCTGTTTGA‑TGGAAGGCACTTGCTACGCTCATCTTGATGAGGTCTGAAATgg < 7:255581/149‑1 (MQ=255) gCCAGCGCCGGGTGGCTGCTACAGTGATATCAATCGCTTGAGGGCGCCACGAGCCTGCTGCACGGTTTTTGCTTAAGTCGGTTTTCAAGACTTGCTGTTTGA‑TGGAAGGCACTTGCTACGCTCATCTTGATGAGGTCTGAAATGGtttt > 2:84169/1‑149 (MQ=255) cgcCGGGTGGCTGCTACAGTTATATAAATCTCTTGAGGGCGCCACGAGCCTGCTGCACGGTTTATGCTTAAGTCGGTTTTCAAGACTTGCTGTTTGA‑TGGAAGGCACTTGCTACGCTCATCTTGATGAGGTCTGAAATGGTTTTCGAAc < 8:187467/149‑1 (MQ=255) cgcCGGGTGGCTGCTACAGTGATATCAATCGCTTGAGGGCGCCACGAGCCTGCTGCACGGTTTTTGCTTAAGTCGGTTTTCAAGACTTGCTGTTTGA‑TGGAAGGCACTTGCTACGCTCATCTTGATGAGGTCTGAAATGGTTTTCGAAc < 1:185284/149‑1 (MQ=255) ccGGGTGGCTGCTACAGTGATATCAATCGCTTGAGGGCGCCACGAGCCTGCTGCACGGTTTTTGCTTAAGTCGGTTTTCAAGACTTGCTGTTTGATTGGAAGGCACTTGCTACGCTCATCTTGATGAGGTCTGAAATGGTTTTCGAAcc < 1:84169/149‑1 (MQ=255) aGTGATATCAATCGCTTGAGGGCGCCACGAGCCTGCTGCACGGTTTTTGCTTAAGTCGGTTTTCAAGACTTGCTGTTTGA‑TGGAAGGCACTTGCTACGCTCATCTTGATGAGGTCTGAAATGGTTTTCGAACCCAGAAGCAGTCGTTcc > 3:303968/1‑149 (MQ=255) aGTGATATCAATCGCTTGAGGGCGCCACGAGCCTGCTGCACGGTTTTTGCTTAAGTCGGTTTTCAAGACTTGCTGTTTGA‑TGGAAGGCACTTGCTACGCTCATCTTGATGAGGTCTGAAATGGTTTTCGAACCCAGAAGCAGTCGTTcc < 2:45560/149‑1 (MQ=255) tGATATCAATCGCTTGAGGGCACCACGAGCCTGCTGCACGGTTTTTGCTTAAGTCGGTTTTCAAGACTTGCTGTTTGA‑TGGAAGGCACTTGCTACGCTCATCTTGATGAGGTCTGAAATGGTTTTCGAACCCAGAAGc > 7:35645/1‑138 (MQ=255) tGATATCAATCGCTTGAGGGCACCACGAGCCTGCTGCACGGTTTTTGCTTAAGTCGGTTTTCAAGACTTGCTGTTTGA‑TGGAAGGCACTTGCTACGCTCATCTTGATGAGGTCTGAAATGGTTTTCGAACCCAGAAGc > 7:40698/1‑138 (MQ=255) tGATATCAATCGCTTGAGGGCACCACGAGCCTGCTGCACGGTTTTTGCTTAAGTCGGTTTTCAAGACTTGCTGTTTGA‑TGGAAGGCACTTGCTACGCTCATCTTGATGAGGTCTGAAATGGTTTTCGAACCCAGAAGc < 8:35645/138‑1 (MQ=255) tGATATCAATCGCTTGAGGGCACCACGAGCCTGCTGCACGGTTTTTGCTTAAGTCGGTTTTCAAGACTTGCTGTTTGA‑TGGAAGGCACTTGCTACGCTCATCTTGATGAGGTCTGAAATGGTTTTCGAACCCAGAAGc < 8:40698/138‑1 (MQ=255) tatCAATCGCTTGAGGGCGCCACGAGCCTGCTGCACGGTTTTTGCTTAAGTCGGTTTTCAAGACTTGCTGTTTGA‑TGGAAGGCACTTGCTACGCTCATCTTGATGAGGTCTGAAATGGTTTTCGAACCCAGAAGCAGTCGTTCCTTACt > 5:333211/1‑149 (MQ=255) atCAATCGCTTGAGGGCGCCACGAGCCTGCTGCACGGTTTTTGCTTAAGTCGGTTTTCAAGACTTGCTGTTTGA‑TGGAAGGCACTTGCTACGCTCATCTTGATGAGGTCTGAAATGGTTTTCGAACCCAGAAGCAGTCGTTCCTTACTc > 2:167780/1‑149 (MQ=255) tCAATCGCTTGAGGGCGACACGAGCCTGCTGCACGTTTTTTGCTTAAGTCGGTTTTCAAGACTTGCTGTTTGA‑TGGAAGGCACTTGCTACGCTCATCTTGATGAGGTCTGAAATGGTTTTCGAACCCAGAAGCAGTCGTTCCTTACTc < 8:171546/148‑1 (MQ=255) aTCGCTTGAGGGCGCCACGAGCCTGCTGCACGGTTTTTGCTTAAGTCGGTTTTCAAGACTTGCTGTTTGA‑TGGAAGGCACTTGCTACGCTCATCTTGATGAGGTCTGAAATGGTTTTCGAACCCAGAAGCAGTCGTTCCTTACTCCa > 3:16625/1‑147 (MQ=255) gCTTGAGGGCGCCACGAGCCTGCTGCACGGTTTTTGCTTAAGTCGGTTTTCAAGACTTGCTGTTTGA‑TGGAAGGCACTTGCTACGCTCATCTTGATGAGGTCTGAAATGGTTTTCGAACCCAGAAGCAGTCGTTCCTTACTCCAGagaa < 6:333211/149‑1 (MQ=255) aGGGCGCCACGAGCCTGCTGCACGGTTTTTGCTTAAGTCGGTTTTCAAGACTTACTGTTTGA‑TGGAAGGCACTTGCTACGCTCATCTTGATGAGGTCTGAAATGGTTTTCGAACCCAGAAGCAGTCGTTCCTTACTCCAGAGAAGAAGc < 2:214358/149‑1 (MQ=255) ggCGCCACGAGCCTGCTGCACGGTTTTTGCTTAAGTCGGTTTTCAAGACTTGCTGTTTGA‑TGGAAGGCACTTGCTACGCTCATCTTGATGAGGTCTGAAATGGTTTTCGAACCCAGAAGCAGTCGTTCCTTACTCCAGAGAAGAAGCAg > 1:178546/1‑149 (MQ=255) | GCTATTCCTGTTGCGTACCCTGTTGTACCGGCGGGCGGCGGTTTCGGCCAGGCGGGCCCTATGAATCGGCCAAAAATCGCAAAAGCGGTGATCCC‑GGGTACGCCAGCGCCGGGTGGCTGCTACAGTGATATCAATCGCTTGAGGGCGCCACGAGCCTGCTGCACGGTTTTTGCTTAAGTCGGTTTTCAAGACTTGCTGTTTGA‑TGGAAGGCACTTGCTACGCTCATCTTGATGAGGTCTGAAATGGTTTTCGAACCCAGAAGCAGTCGTTCCTTACTCCAGAGAAGAAGCAG > NC_002947/3557460‑3557751 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |