Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,633,135 C→T 11.4% L203L (CTG→TTG)  PP_3201 → BNR domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,633,1350CT11.4% 91.5 / 3.6 35L203L (CTG→TTG) PP_3201BNR domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (17/14);  new base T (2/2);  total (19/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

GCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCAGCTACTTCGCCGCCACCGTGTTGCCCGATGGCCGTCTGC  >  NC_002947/3632997‑3633282
                                                                                                                                          |                                                                                                                                                   
gCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGcc                                                                                                                                            <  8:242437/148‑1 (MQ=255)
   cTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGGTTGCCTTGGCGCGCCAggg                                                                                                                                        >  5:301979/1‑149 (MQ=255)
        tttCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAAc                                                                                                                                    >  1:209393/1‑148 (MQ=255)
         ttCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAAc                                                                                                                                    >  3:40942/1‑147 (MQ=255)
             ccGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGGTTGCCTTGGCGCGCCAGGGCATACAGgtg                                                                                                                              <  6:301979/149‑1 (MQ=39)
                   cccGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTACACCTGGTTGCCTTGGCGCGCCAGGGCATACAGgtgcagtc                                                                                                                         <  4:236292/148‑6 (MQ=37)
                    ccGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCg                                                                                                                       >  6:156661/1‑149 (MQ=255)
                        aaCGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCgg                                                                                                                   >  2:260089/1‑149 (MQ=255)
                                 gtcgtcGGTGCCTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAgg                                                                                                           <  2:209393/148‑1 (MQ=255)
                                         tGCCTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCtgctgc                                                                                                  <  6:279709/149‑1 (MQ=255)
                                                ggggTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTtcgctc                                                                                           <  5:156661/149‑1 (MQ=255)
                                                  ggTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTtcgctcgc                                                                                         <  4:40942/149‑1 (MQ=255)
                                                  ggTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTtcgctcgc                                                                                         <  1:260089/149‑1 (MQ=255)
                                                                 cgacgaCGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGT‑GACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCa                                                                         >  6:169816/1‑149 (MQ=255)
                                                                     gacgGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCAGTa                                                                      >  6:93360/1‑149 (MQ=255)
                                                                                        aGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGgcgc                                                              <  8:170321/138‑1 (MQ=255)
                                                                                        aGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGgcgc                                                              >  7:170321/1‑138 (MQ=255)
                                                                                          tCGGAGATCGGCCGACTGCCGAACGGGCGT‑GACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCt                                                  <  5:169816/147‑1 (MQ=255)
                                                                                             gagaTCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGCGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGa                                                                            <  1:117960/119‑1 (MQ=255)
                                                                                             gagaTCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGCGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGa                                                                            >  2:117960/1‑119 (MQ=255)
                                                                                                aTCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGgc                                                                <  6:31824/128‑1 (MQ=255)
                                                                                                    gCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCtt                                                                                                >  2:55256/1‑92 (MQ=255)
                                                                                                    gCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCtt                                                                                                <  1:55256/92‑1 (MQ=255)
                                                                                                    gCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATg                                            >  1:295057/1‑144 (MQ=255)
                                                                                                    gCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATg                                            <  2:295057/144‑1 (MQ=255)
                                                                                                    gCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCAGc                                       >  1:247999/1‑149 (MQ=255)
                                                                                                                  gggCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAg                                                                  <  3:250109/108‑1 (MQ=255)
                                                                                                                  gggCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAg                                                                  >  4:250109/1‑108 (MQ=255)
                                                                                                                  gggCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCAGCTACTTCGCCGCCAc                         >  7:67667/1‑149 (MQ=255)
                                                                                                                    gCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCAGCTACTTCGCCGCCACCg                       >  7:179403/1‑149 (MQ=255)
                                                                                                                                 ctggttGCCTTGGCGCGCCAGGGCATACAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCAGCTACTTCGCCGCCACCGTGTTGCCCGATgg          >  5:216622/6‑149 (MQ=255)
                                                                                                                                 cTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGAt                                             <  7:136005/114‑1 (MQ=255)
                                                                                                                                 cTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGAt                                             >  8:136005/1‑114 (MQ=255)
                                                                                                                                  tGTATTCACTGGCGCGCCAAGGCAACCAGGAGTACATCGCCGGCTAGCAGGGCCTGCTGCTTCGCTCGCCTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCAGCTACTTAGCCGCCACCGTCTTCCCCGATGGc         >  5:224571/1‑149 (MQ=255)
                                                                                                                                     aTGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCAGCTACTTCGCCGCCACCGTGTTGCCCGATGGCCGt      >  2:310646/1‑149 (MQ=255)
                                                                                                                                        cccTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCAGCTACTTCGCCGCCACCGTGTTGCCCGATGGCCGTctg   >  8:27339/1‑149 (MQ=255)
                                                                                                                                        cccTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCAGCTACTTCGCCGCCACCGTGTTGCCCGATGGCCGTctg   <  2:247999/149‑1 (MQ=255)
                                                                                                                                         ccTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCAGCTACTTCGCCGCCACCGTGTTGCCCGATGGCCGTctgc  >  5:216912/1‑149 (MQ=255)
                                                                                                                                         ccTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCAGCTACTTCGCCGCCACCGTGTTGCCCGATGGCCGTctgc  >  1:121472/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                                   
GCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCAGCTACTTCGCCGCCACCGTGTTGCCCGATGGCCGTCTGC  >  NC_002947/3632997‑3633282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑