Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,641,239 T→G 9.1% D180A (GAC→GCC)  PP_3208 ← Pdr/VanB family oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,641,2390TG 9.1% 126.5 / 4.5 44D180A (GAC→GCC) PP_3208Pdr/VanB family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (20/20);  new base G (2/2);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

CAGTGACGCTCGACGTGCAAGGTGGGCTCTGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAAGCGCACCGACCGACCTTG  >  NC_002947/3641092‑3641387
                                                                                                                                                   |                                                                                                                                                    
cAGTGACGCTCGACGTGCAAGGTGGGCTCTGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGt                                                                                                                                                      <  2:106381/148‑1 (MQ=255)
     aCGCTCGACGTGCAAGGTGGGCTCTGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAgg                                                                                                                                                >  6:95827/1‑149 (MQ=255)
     aCGCTCGACGTGCAAGGTGGGCTCTGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAg                                                                                                                                                 <  5:300754/148‑1 (MQ=255)
      cGCTCGACGTGCAAGGTGGGCTCTGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGc                                                                                                                                               <  6:44699/149‑1 (MQ=255)
           gACGTGCAAGGTGGGCTCTGATGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGc                                                                                                                                           <  6:255924/148‑1 (MQ=255)
           gACGTGCAAGGTGGGCTCTGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGc                                                                                                                                           <  2:16085/148‑1 (MQ=255)
                    ggTGGGCTCTGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTtcct                                                                                                                                 <  3:136932/149‑1 (MQ=255)
                        ggCTCTGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAg                                                                                                                             >  8:240013/1‑149 (MQ=255)
                               aGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTAcc                                                                                                                        <  3:248214/147‑1 (MQ=255)
                                   cTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGa                                                                                                                  >  8:207733/1‑149 (MQ=255)
                                          tGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCgg                                                                                                           <  4:101963/149‑1 (MQ=255)
                                           gTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCggg                                                                                                          >  5:141941/1‑149 (MQ=255)
                                               gcgGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGgcgc                                                                                                      >  2:303929/1‑149 (MQ=255)
                                                   tGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCaa                                                                                                  <  7:240013/149‑1 (MQ=255)
                                                         gcCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTg                                                                                             >  3:116833/1‑148 (MQ=255)
                                                           cGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCAt                                                                                          <  1:303929/149‑1 (MQ=255)
                                                               gTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAAc                                                                                      <  5:95827/149‑1 (MQ=255)
                                                                    ccAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCgg                                                                                 >  4:288263/1‑149 (MQ=255)
                                                                     cAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGAGATCATCGGGGCGCGCACGCGTGCATGACCCTCgg                                                                                 >  8:275994/1‑148 (MQ=255)
                                                                              gCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATc                                                                       >  4:59725/1‑149 (MQ=255)
                                                                               cAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCg                                                                      <  4:116833/149‑1 (MQ=255)
                                                                                aaGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGaa                                                                                       >  6:221944/1‑131 (MQ=255)
                                                                                aaGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGaa                                                                                       <  5:221944/131‑1 (MQ=255)
                                                                                               gACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCGGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATaaaa                                                       <  7:95449/148‑1 (MQ=255)
                                                                                                    acGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCAcc                                                 >  6:233207/1‑149 (MQ=255)
                                                                                                    acGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCAcc                                                 >  8:135390/1‑149 (MQ=255)
                                                                                                    acGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCAcc                                                 >  8:215700/1‑149 (MQ=255)
                                                                                                          tGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCg                                           <  6:188461/149‑1 (MQ=255)
                                                                                                               ccGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATc                                      >  3:167711/1‑149 (MQ=255)
                                                                                                                   cAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCgg                                  <  7:215700/149‑1 (MQ=255)
                                                                                                                          acaGCAATAGAGGCTTGCGCTGCCGTCGCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGaaa                            >  2:12570/1‑148 (MQ=255)
                                                                                                                          acaGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGaaa                            >  7:164008/1‑148 (MQ=255)
                                                                                                                              cAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAg                       <  3:59725/149‑1 (MQ=255)
                                                                                                                               aaTAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGTGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACacc                      <  7:207733/149‑3 (MQ=255)
                                                                                                                                    agGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAAgcg                 <  7:135390/149‑1 (MQ=255)
                                                                                                                                           acttGCCGGCCTGGGACGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATaa                                                         >  1:107148/4‑102 (MQ=255)
                                                                                                                                           acttGCCGGCCTGGGACGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATaa                                                         <  2:107148/99‑1 (MQ=255)
                                                                                                                                           acttGCCGGCCTGGGACGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATaa                                                         <  2:103384/99‑1 (MQ=255)
                                                                                                                                           acttGCCGGCCTGGGACGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATaa                                                         >  1:103384/4‑102 (MQ=255)
                                                                                                                                            gcTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGc                      <  6:40418/136‑1 (MQ=255)
                                                                                                                                            gcTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGc                      >  5:40418/1‑136 (MQ=255)
                                                                                                                                            gcTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAAGCGCaccgacc         >  4:309407/1‑149 (MQ=255)
                                                                                                                                            gcTGCCGTCCCAGGCCGGCAAGTGTTAATCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAGGCGCaccgacc         >  1:15446/1‑149 (MQ=255)
                                                                                                                                                ccGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAAGCGCACggaccgac      <  5:80318/148‑1 (MQ=255)
                                                                                                                                                ccGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAAGCGCACcgaccgac      >  6:80318/1‑148 (MQ=255)
                                                                                                                                                   tCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGGGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAAGCGCACCGACCGACCTTg  <  7:275994/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CAGTGACGCTCGACGTGCAAGGTGGGCTCTGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAAGCGCACCGACCGACCTTG  >  NC_002947/3641092‑3641387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑