Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,641,243 A→G 8.3% W179R (TGG→CGG) ‡ PP_3208 ← Pdr/VanB family oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,641,2430AG 8.3% 135.3 / 2.8 48W179R (TGG→CGG) ‡PP_3208Pdr/VanB family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (22/22);  new base G (2/2);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.79e-01

ACGCTCGACGTGCAAGGTGGGCTCTGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAAGCGCACCGACCGACCTTGCG  >  NC_002947/3641097‑3641389
                                                                                                                                                  |                                                                                                                                                  
aCGCTCGACGTGCAAGGTGGGCTCTGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAgg                                                                                                                                                  >  6:95827/1‑149 (MQ=255)
aCGCTCGACGTGCAAGGTGGGCTCTGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAg                                                                                                                                                   <  5:300754/148‑1 (MQ=255)
 cGCTCGACGTGCAAGGTGGGCTCTGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGc                                                                                                                                                 <  6:44699/149‑1 (MQ=255)
      gACGTGCAAGGTGGGCTCTGATGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGc                                                                                                                                             <  6:255924/148‑1 (MQ=255)
      gACGTGCAAGGTGGGCTCTGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGc                                                                                                                                             <  2:16085/148‑1 (MQ=255)
               ggTGGGCTCTGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTtcct                                                                                                                                   <  3:136932/149‑1 (MQ=255)
                   ggCTCTGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAg                                                                                                                               >  8:240013/1‑149 (MQ=255)
                          aGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTAcc                                                                                                                          <  3:248214/147‑1 (MQ=255)
                              cTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGa                                                                                                                    >  8:207733/1‑149 (MQ=255)
                                     tGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCgg                                                                                                             <  4:101963/149‑1 (MQ=255)
                                      gTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCggg                                                                                                            >  5:141941/1‑149 (MQ=255)
                                          gcgGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGgcgc                                                                                                        >  2:303929/1‑149 (MQ=255)
                                              tGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCaa                                                                                                    <  7:240013/149‑1 (MQ=255)
                                                    gcCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTg                                                                                               >  3:116833/1‑148 (MQ=255)
                                                      cGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCAt                                                                                            <  1:303929/149‑1 (MQ=255)
                                                          gTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAAc                                                                                        <  5:95827/149‑1 (MQ=255)
                                                               ccAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCgg                                                                                   >  4:288263/1‑149 (MQ=255)
                                                                cAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGAGATCATCGGGGCGCGCACGCGTGCATGACCCTCgg                                                                                   >  8:275994/1‑148 (MQ=255)
                                                                         gCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATc                                                                         >  4:59725/1‑149 (MQ=255)
                                                                          cAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCg                                                                        <  4:116833/149‑1 (MQ=255)
                                                                           aaGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGaa                                                                                         <  5:221944/131‑1 (MQ=255)
                                                                           aaGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGaa                                                                                         >  6:221944/1‑131 (MQ=255)
                                                                                          gACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCGGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATaaaa                                                         <  7:95449/148‑1 (MQ=255)
                                                                                               acGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCAcc                                                   >  8:215700/1‑149 (MQ=255)
                                                                                               acGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCAcc                                                   >  8:135390/1‑149 (MQ=255)
                                                                                               acGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCAcc                                                   >  6:233207/1‑149 (MQ=255)
                                                                                                     tGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCg                                             <  6:188461/149‑1 (MQ=255)
                                                                                                          ccGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATc                                        >  3:167711/1‑149 (MQ=255)
                                                                                                              cAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCgg                                    <  7:215700/149‑1 (MQ=255)
                                                                                                                     acaGCAATAGAGGCTTGCGCTGCCGTCGCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGaaa                              >  2:12570/1‑148 (MQ=255)
                                                                                                                     acaGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGaaa                              >  7:164008/1‑148 (MQ=255)
                                                                                                                         cAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAg                         <  3:59725/149‑1 (MQ=255)
                                                                                                                          aaTAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGTGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACacc                        <  7:207733/149‑3 (MQ=255)
                                                                                                                               agGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAAgcg                   <  7:135390/149‑1 (MQ=255)
                                                                                                                                      acttGCCGGCCTGGGACGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATaa                                                           >  1:107148/4‑102 (MQ=255)
                                                                                                                                      acttGCCGGCCTGGGACGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATaa                                                           >  1:103384/4‑102 (MQ=255)
                                                                                                                                      acttGCCGGCCTGGGACGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATaa                                                           <  2:103384/99‑1 (MQ=255)
                                                                                                                                      acttGCCGGCCTGGGACGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATaa                                                           <  2:107148/99‑1 (MQ=255)
                                                                                                                                       gcTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGc                        <  6:40418/136‑1 (MQ=255)
                                                                                                                                       gcTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGc                        >  5:40418/1‑136 (MQ=255)
                                                                                                                                       gcTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAAGCGCaccgacc           >  4:309407/1‑149 (MQ=255)
                                                                                                                                       gcTGCCGTCCCAGGCCGGCAAGTGTTAATCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAGGCGCaccgacc           >  1:15446/1‑149 (MQ=255)
                                                                                                                                           ccGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAAGCGCACggaccgac        <  5:80318/148‑1 (MQ=255)
                                                                                                                                           ccGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAAGCGCACcgaccgac        >  6:80318/1‑148 (MQ=255)
                                                                                                                                              tCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGGGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAAGCGCACCGACCGACCTTg    <  7:275994/149‑1 (MQ=255)
                                                                                                                                               cccAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCg                                                                        <  1:249368/80‑1 (MQ=255)
                                                                                                                                               cccAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCg                                                                        >  2:249368/1‑80 (MQ=255)
                                                                                                                                                ccAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAAGCGCACCGACCGACCTTgcg  >  2:13828/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ACGCTCGACGTGCAAGGTGGGCTCTGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAAGCGCACCGACCGACCTTGCG  >  NC_002947/3641097‑3641389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑