Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,641,246 C→A 8.4% A178S (GCC→TCC)  PP_3208 ← Pdr/VanB family oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,641,2460CA 8.4% 143.7 / 2.1 46A178S (GCC→TCC) PP_3208Pdr/VanB family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (21/21);  new base A (2/2);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

CGCTCGACGTGCAAGGTGGGCTCTGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAAGCGCACCGACCGACCTTGCGCTT  >  NC_002947/3641098‑3641392
                                                                                                                                                    |                                                                                                                                                  
cGCTCGACGTGCAAGGTGGGCTCTGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGc                                                                                                                                                    <  6:44699/149‑1 (MQ=255)
     gACGTGCAAGGTGGGCTCTGATGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGc                                                                                                                                                <  6:255924/148‑1 (MQ=255)
     gACGTGCAAGGTGGGCTCTGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGc                                                                                                                                                <  2:16085/148‑1 (MQ=255)
              ggTGGGCTCTGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTtcct                                                                                                                                      <  3:136932/149‑1 (MQ=255)
                  ggCTCTGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAg                                                                                                                                  >  8:240013/1‑149 (MQ=255)
                         aGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTAcc                                                                                                                             <  3:248214/147‑1 (MQ=255)
                             cTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGa                                                                                                                       >  8:207733/1‑149 (MQ=255)
                                    tGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCgg                                                                                                                <  4:101963/149‑1 (MQ=255)
                                     gTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCggg                                                                                                               >  5:141941/1‑149 (MQ=255)
                                         gcgGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGgcgc                                                                                                           >  2:303929/1‑149 (MQ=255)
                                             tGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCaa                                                                                                       <  7:240013/149‑1 (MQ=255)
                                                   gcCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTg                                                                                                  >  3:116833/1‑148 (MQ=255)
                                                     cGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCAt                                                                                               <  1:303929/149‑1 (MQ=255)
                                                         gTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAAc                                                                                           <  5:95827/149‑1 (MQ=255)
                                                              ccAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCgg                                                                                      >  4:288263/1‑149 (MQ=255)
                                                               cAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGAGATCATCGGGGCGCGCACGCGTGCATGACCCTCgg                                                                                      >  8:275994/1‑148 (MQ=255)
                                                                        gCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATc                                                                            >  4:59725/1‑149 (MQ=255)
                                                                         cAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCg                                                                           <  4:116833/149‑1 (MQ=255)
                                                                          aaGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGaa                                                                                            <  5:221944/131‑1 (MQ=255)
                                                                          aaGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGaa                                                                                            >  6:221944/1‑131 (MQ=255)
                                                                                         gACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCGGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATaaaa                                                            <  7:95449/148‑1 (MQ=255)
                                                                                              acGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCAcc                                                      >  6:233207/1‑149 (MQ=255)
                                                                                              acGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCAcc                                                      >  8:215700/1‑149 (MQ=255)
                                                                                              acGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCAcc                                                      >  8:135390/1‑149 (MQ=255)
                                                                                                    tGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCg                                                <  6:188461/149‑1 (MQ=255)
                                                                                                         ccGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATc                                           >  3:167711/1‑149 (MQ=255)
                                                                                                             cAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCgg                                       <  7:215700/149‑1 (MQ=255)
                                                                                                                    acaGCAATAGAGGCTTGCGCTGCCGTCGCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGaaa                                 >  2:12570/1‑148 (MQ=255)
                                                                                                                    acaGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGaaa                                 >  7:164008/1‑148 (MQ=255)
                                                                                                                        cAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAg                            <  3:59725/149‑1 (MQ=255)
                                                                                                                         aaTAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGTGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACacc                           <  7:207733/149‑3 (MQ=255)
                                                                                                                              agGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAAgcg                      <  7:135390/149‑1 (MQ=255)
                                                                                                                                     acttGCCGGCCTGGGACGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATaa                                                              >  1:107148/4‑102 (MQ=255)
                                                                                                                                     acttGCCGGCCTGGGACGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATaa                                                              >  1:103384/4‑102 (MQ=255)
                                                                                                                                     acttGCCGGCCTGGGACGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATaa                                                              <  2:103384/99‑1 (MQ=255)
                                                                                                                                     acttGCCGGCCTGGGACGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATaa                                                              <  2:107148/99‑1 (MQ=255)
                                                                                                                                      gcTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGc                           <  6:40418/136‑1 (MQ=255)
                                                                                                                                      gcTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGc                           >  5:40418/1‑136 (MQ=255)
                                                                                                                                      gcTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAAGCGCaccgacc              >  4:309407/1‑149 (MQ=255)
                                                                                                                                      gcTGCCGTCCCAGGCCGGCAAGTGTTAATCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAGGCGCaccgacc              >  1:15446/1‑149 (MQ=255)
                                                                                                                                          ccGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAAGCGCACggaccgac           <  5:80318/148‑1 (MQ=255)
                                                                                                                                          ccGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAAGCGCACcgaccgac           >  6:80318/1‑148 (MQ=255)
                                                                                                                                             tCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGGGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAAGCGCACCGACCGACCTTg       <  7:275994/149‑1 (MQ=255)
                                                                                                                                              cccAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCg                                                                           <  1:249368/80‑1 (MQ=255)
                                                                                                                                              cccAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCg                                                                           >  2:249368/1‑80 (MQ=255)
                                                                                                                                               ccAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAAGCGCACCGACCGACCTTgcg     >  2:13828/1‑149 (MQ=255)
                                                                                                                                                  ggCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAAGCGCACCGACCGACCTTGCGCtt  <  3:309407/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGCTCGACGTGCAAGGTGGGCTCTGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTACCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAAGCGCACCGACCGACCTTGCGCTT  >  NC_002947/3641098‑3641392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑