Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,667,243 A→G 8.7% intergenic (+186/+76) PP_3230 → / ← PP_3231 phosphoribosyl transferase domain‑containing protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,667,2430AG 8.7% 155.7 / 4.4 57intergenic (+186/+76)PP_3230/PP_3231phosphoribosyl transferase domain‑containing protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (25/27);  new base G (2/3);  total (27/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

CGTCGAACTCGTGGCGGGCGTCTGAGCGCCCGCAATCAATACGTAGCAGACGCTCTCGCGGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTCGGCAAGGGCAGG  >  NC_002947/3667100‑3667382
                                                                                                                                               |                                                                                                                                           
cGTCGAACTCGTGGCGGGCGTCTGAGCGCCCGCAATCAATACGTAGCAGACGCTCTCGCGGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCcg                                                                                                                                        >  5:68502/1‑149 (MQ=255)
  tCGAACTCGTGGCGGGCGTCTGAGCGCCCGCAATCAATACGTAGCAGACGCTCTCGCGGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCcgcg                                                                                                                                      <  1:297775/149‑1 (MQ=255)
       cTCGTGGCGGGCGTCTGAGCGCCCGCAATCAATACGTAGCAGACGCTCTCGCGGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCt                                                                                                                                 >  1:201783/1‑149 (MQ=255)
          gTGGCGGGCGTCTGAGCGCCCGCAATCAATACGTAGCAGACGCTCTCGCGGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAGCGCCGCGGCCTTCGc                                                                                                                              <  1:311772/149‑1 (MQ=255)
          gTGGCGGGCGTCTGAGCGCCCGCAATCAATACGTAGCAGACGCTCTCGCGGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGc                                                                                                                              >  2:220274/1‑149 (MQ=255)
             gcgggcgTCTGAGCGCCCGCAATCAATACGTAGCAGACGCTCTCGCGGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCg                                                                                                                            <  2:102926/148‑1 (MQ=255)
               gggcgTCTGAGCGCCCGCAATCAATACGTAGCAGACGCTCTCGCGGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAGCGCCGCGGCCTTCGccaa                                                                                                                           >  7:207869/1‑145 (MQ=255)
               gggcgTCTGAGCGCCCGCAATCAATACGTAGCAGACGCTCTCGCGGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAGCGCCGCGGCCTTCGccaa                                                                                                                           <  8:207869/147‑3 (MQ=255)
               gggcgTCTGAGCGCCCGCAATCAATACGTAGCAGACGCTCTCGCGGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGt                                                                                                                          >  5:86463/1‑148 (MQ=255)
                ggcgTCTGAGCGCCCGCAATCAATACGTAGCAGACGCTCTCGCGGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAGCGCCGCGGCCTTCGccaaggt                                                                                                                        <  2:100775/149‑6 (MQ=255)
                  cgTCTGAGCGCCCGCAATCAATACGTAGCAGACGCTCTCGCGGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACt                                                                                                                      <  6:222339/149‑1 (MQ=255)
                   gTCTGAGCGCCCGCAATCAATACGTAGCAGACGCTCTCGCGGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGTTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTg                                                                                                                     <  1:75652/149‑1 (MQ=255)
                   gTCTGAGCGCCCGCAATCAATACGTAGCAGACGCTCTCGCGGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTg                                                                                                                     <  6:68502/149‑1 (MQ=255)
                     cTGAGCGCCCGCAATCAATACGTAGCAGACGCTCTCGCGGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGt                                                                                                                   >  4:5566/1‑149 (MQ=255)
                       gAGCGCCCGCAATCAATACGTAGCAGACGCTCTCGCGGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTc                                                                                                                 >  2:178548/1‑149 (MQ=255)
                        aGCGCCCGCAATCAATACGTAGCAGACGCTCTCGCGGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTc                                                                                                                 <  2:201783/148‑1 (MQ=255)
                         gcgcCCGCAATCAATACGTAGCAGACGCTCTCGCGGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGt                                                                                                               >  6:137054/1‑149 (MQ=255)
                              cGCAATCAATACGTAGCAGACGCTCTCGCGGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGt                                                                                                          <  3:45168/149‑1 (MQ=255)
                                caatcaatACGTAGCAGACGCTCTCGCGGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTAc                                                                                                        <  3:5566/149‑1 (MQ=255)
                                 aatcaatACGTAGCAGACGCTCTCGCGGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTAcc                                                                                                       >  3:229195/1‑149 (MQ=255)
                                              cAGACGCTCTCGCGGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATc                                                                                          >  6:243346/1‑149 (MQ=255)
                                               aGACGCTCTCGCGGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATcc                                                                                         <  5:137054/149‑1 (MQ=255)
                                                 aCGCTCTCGCGGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGt                                                                                       >  5:60908/1‑149 (MQ=255)
                                                         gcgGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATggg                                                                               >  3:276661/1‑149 (MQ=255)
                                                          cgGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATgggg                                                                              <  6:86463/149‑1 (MQ=255)
                                                              aCGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTAc                                                                                                        >  3:78343/1‑119 (MQ=255)
                                                              aCGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTAc                                                                                                        <  4:78343/119‑1 (MQ=255)
                                                              aCGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAgc                                                                                                 <  3:73789/126‑1 (MQ=255)
                                                              aCGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAgc                                                                                                 >  4:73789/1‑126 (MQ=255)
                                                                           tCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATa                                                             <  3:131180/149‑1 (MQ=255)
                                                                             actactCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCcg                                                                                                                                        <  1:23476/72‑1 (MQ=255)
                                                                             actactCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCcg                                                                                                                                        >  2:23476/1‑72 (MQ=255)
                                                                               tactCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATacac                                                          >  3:114322/1‑148 (MQ=255)
                                                                                   ctTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAAt                                                     >  8:289750/1‑149 (MQ=255)
                                                                                    ttCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCaaa                                                      <  2:81095/147‑1 (MQ=255)
                                                                                            gctgAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGtt                                            >  3:27201/1‑149 (MQ=255)
                                                                                             ctgAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTTGCACCTTGGCGAAGGCCGCGGCACTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGttt                                           >  1:219434/1‑149 (MQ=255)
                                                                                             ctgAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGttt                                           <  4:114322/149‑1 (MQ=255)
                                                                                              tgAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTc                                          >  1:174803/1‑149 (MQ=255)
                                                                                                  cTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTcc                                      <  4:229195/149‑1 (MQ=255)
                                                                                                     aaTCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTAcc                                                                                                       <  5:317089/81‑1 (MQ=255)
                                                                                                     aaTCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTAcc                                                                                                       >  6:317089/1‑81 (MQ=255)
                                                                                                     aaTCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACAcc                                                         <  8:270733/127‑1 (MQ=255)
                                                                                                     aaTCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACAcc                                                         >  7:270733/1‑127 (MQ=255)
                                                                                                     aaTCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCctct                                   >  7:314/1‑149 (MQ=255)
                                                                                                     aaTCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCctc                                    <  6:60908/148‑1 (MQ=255)
                                                                                                       tCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTgg                                 <  5:243346/149‑1 (MQ=255)
                                                                                                         gTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCt                               >  8:92901/1‑149 (MQ=255)
                                                                                                              gTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGt                                             >  8:291421/1‑130 (MQ=255)
                                                                                                              gTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGt                                             <  7:291421/130‑1 (MQ=255)
                                                                                                                   ccGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCtg                     <  1:178548/149‑1 (MQ=255)
                                                                                                                    cGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCtgt                    >  4:156000/1‑149 (MQ=255)
                                                                                                                          aaGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGTATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTc              <  1:220274/149‑1 (MQ=255)
                                                                                                                           agagCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTCg             <  7:92901/149‑1 (MQ=255)
                                                                                                                             agCCTGGCACCTTGGCGAAGGCCGCGGCACTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTCGGc           <  2:219434/149‑1 (MQ=255)
                                                                                                                               ccTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTCGGCaa         <  3:145793/149‑1 (MQ=255)
                                                                                                                                      caccggCGAGCGCCGCGGCCTTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTCATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCGCTGGCTCAAGATGCTGTTGGTTCGGAAagggcagg  >  6:304352/5‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                           
CGTCGAACTCGTGGCGGGCGTCTGAGCGCCCGCAATCAATACGTAGCAGACGCTCTCGCGGCACGTGGCCTAAGGTCACTACTCTTCGACCTGCTGAGCTCAATCGTCAGGTGGACCGCTCGAAGAGCCTGGCACCTTGGCGAAGGCCGCGGCGCTCGCCGGTGACTGGTTCGTGAAGTACCTGCAGCGCATATCCGTCTGATGGGGCTTGGCCGAGGCTCATACACCAAATGGATAGGTTTCGTCCTCTGGCTCAAGATGCTGTTGTTTCGGCAAGGGCAGG  >  NC_002947/3667100‑3667382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑